Plant Circular RNA Database

Detail information of Os06g0354500_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os06g0354500_circ_g.2
					
						ID in PlantcircBase
						osa_circ_030792
					
						Alias
						Os_ciR1443
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr6: 14508057-14508994  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os06g0354500						
					
						Parent gene annotation
						
							Similar to Acyl-coenzyme A oxidase 3, peroxisomal precursor (EC 
1.3.3.6) (AOX 3) (Medium-chain acyl-CoA oxidase) (AtCX3). (Os06t
0354500-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os06g0354500_circ_g.3 						
					
						Support reads
						12/1
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os06t0354500-01:5

General Information
CircRNA Name	Os06g0354500_circ_g.2
ID in PlantcircBase	osa_circ_030792
Alias	Os_ciR1443
Organism	Oryza sativa
Position	chr6: 14508057-14508994 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os06g0354500
Parent gene annotation	Similar to Acyl-coenzyme A oxidase 3, peroxisomal precursor (EC 1.3.3.6) (AOX 3) (Medium-chain acyl-CoA oxidase) (AtCX3). (Os06t 0354500-01)
Parent gene strand	-
Alternative splicing	Os06g0354500_circ_g.3
Support reads	12/1
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os06t0354500-01:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TTCTGTCATGGAAAAACAACCCTTGATAACATAATTTTCTGTGTCCGACAACCATTTGTCATGG
TGGCGCTTTGTTCCAAGAAACTTGATTGCGCTGCCCctttgaaatataaaccgcattaactgca
atattaactcgaccaagtgtaagtggagataaaaatgctgcaaacctctgcagaacagaaaatt
atcagttt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 574
Transcript exons: 14508057-14508131,14508243-14508342,14508416-14508588,14
508677-14508784,14508877-14508994
CTTTGAAATATAAACCGCATTAACTGCAATATTAACTCGACCAAGTGTAAGTGGAGATAAAAAT
GCTGCAAACCTCTGATCAGGGTCATCTATCGTGCTAACATATTGCCCATCCGGCAAAACATCAG
CAACAAGATTCAGCAAATTCTCTCGAGGAACACGTATATTGTTAAACCAAATTCGTCCATTATC
AACACCATTTAATCCAATTTTATGGCCACAGTCAGCTATCTGGATATTAGGCATAACATTTTCA
TGTTCATCCCTAATCTGAGCTACAAAAGCATGGACTCCTTCATTTCTCCCATTTATATGGAGCT
GCGCAAAGACTATAGTATGTGTAGCATGATTAGCAGCTCCACCAATCCAGTACTTCTGGGCTGA
TTCACAAGGAGTATTTATCACAAACTCTCTTGTTTTAATATCATAAGTAGCCACTGTCTCAATT
CCTCGGACATTGCTCCCATGACCCAGTTCTGTCATGGAAAAACAACCCTTGATAACATAATTTT
CTGTGTCCGACAACCATTTGTCATGGTGGCGCTTTGTTCCAAGAAACTTGATTGCGCTGCCC
						
					

						Genomic sequence
						
							CTTTGAAATATAAACCGCATTAACTGCAATATTAACTCGACCAAGTGTAAGTGGAGATAAAAAT
GCTGCAAACCTCTGCAGAACAGAAAATTATCAGTTTGAAAATGAGACAAAAATGAATGCAGCAA
CTACTTATCTATGTGTACGTCTAGGGAGTGAGGGAGAGAGAGGATAAGGTCGCATTACCTGATC
AGGGTCATCTATCGTGCTAACATATTGCCCATCCGGCAAAACATCAGCAACAAGATTCAGCAAA
TTCTCTCGAGGAACACGTATATTGTTAAACCTGACAGACAGTTAAATTAATTTACAATGTGCGT
GATAGGAAGACAAAACTTTACAGAAGTACAAGGGCTCACCAAATTCGTCCATTATCAACACCAT
TTAATCCAATTTTATGGCCACAGTCAGCTATCTGGATATTAGGCATAACATTTTCATGTTCATC
CCTAATCTGAGCTACAAAAGCATGGACTCCTTCATTTCTCCCATTTATATGGAGCTGCGCAAAG
ACTATAGTATGTGTAGCATGCTGTAAAAGGCCACAAACACCAACAGTAAGTAATAACCAAATAG
ACAGCATAGATGTACTCAATTATTTAAGTGAAATTTCATCATACATTAGCAGCTCCACCAATCC
AGTACTTCTGGGCTGATTCACAAGGAGTATTTATCACAAACTCTCTTGTTTTAATATCATAAGT
AGCCACTGTCTCAATTCCTCGGACCTTCAAATTAGTCAAATAAGGCAAAGAAAAATCTCATCAA
CACAGCTAACAAAACTACAATTCAGTAATTTGACCAAAATATCATCACATACATTGCTCCCATG
ACCCAGTTCTGTCATGGAAAAACAACCCTTGATAACATAATTTTCTGTGTCCGACAACCATTTG
TCATGGTGGCGCTTTGTTCCAAGAAACTTGATTGCGCTGCCC
						
					

			
		

Sequences
Splice junction sequence	TTCTGTCATGGAAAAACAACCCTTGATAACATAATTTTCTGTGTCCGACAACCATTTGTCATGG TGGCGCTTTGTTCCAAGAAACTTGATTGCGCTGCCCctttgaaatataaaccgcattaactgca atattaactcgaccaagtgtaagtggagataaaaatgctgcaaacctctgcagaacagaaaatt atcagttt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 574 Transcript exons: 14508057-14508131,14508243-14508342,14508416-14508588,14 508677-14508784,14508877-14508994 CTTTGAAATATAAACCGCATTAACTGCAATATTAACTCGACCAAGTGTAAGTGGAGATAAAAAT GCTGCAAACCTCTGATCAGGGTCATCTATCGTGCTAACATATTGCCCATCCGGCAAAACATCAG CAACAAGATTCAGCAAATTCTCTCGAGGAACACGTATATTGTTAAACCAAATTCGTCCATTATC AACACCATTTAATCCAATTTTATGGCCACAGTCAGCTATCTGGATATTAGGCATAACATTTTCA TGTTCATCCCTAATCTGAGCTACAAAAGCATGGACTCCTTCATTTCTCCCATTTATATGGAGCT GCGCAAAGACTATAGTATGTGTAGCATGATTAGCAGCTCCACCAATCCAGTACTTCTGGGCTGA TTCACAAGGAGTATTTATCACAAACTCTCTTGTTTTAATATCATAAGTAGCCACTGTCTCAATT CCTCGGACATTGCTCCCATGACCCAGTTCTGTCATGGAAAAACAACCCTTGATAACATAATTTT CTGTGTCCGACAACCATTTGTCATGGTGGCGCTTTGTTCCAAGAAACTTGATTGCGCTGCCC
Genomic sequence	CTTTGAAATATAAACCGCATTAACTGCAATATTAACTCGACCAAGTGTAAGTGGAGATAAAAAT GCTGCAAACCTCTGCAGAACAGAAAATTATCAGTTTGAAAATGAGACAAAAATGAATGCAGCAA CTACTTATCTATGTGTACGTCTAGGGAGTGAGGGAGAGAGAGGATAAGGTCGCATTACCTGATC AGGGTCATCTATCGTGCTAACATATTGCCCATCCGGCAAAACATCAGCAACAAGATTCAGCAAA TTCTCTCGAGGAACACGTATATTGTTAAACCTGACAGACAGTTAAATTAATTTACAATGTGCGT GATAGGAAGACAAAACTTTACAGAAGTACAAGGGCTCACCAAATTCGTCCATTATCAACACCAT TTAATCCAATTTTATGGCCACAGTCAGCTATCTGGATATTAGGCATAACATTTTCATGTTCATC CCTAATCTGAGCTACAAAAGCATGGACTCCTTCATTTCTCCCATTTATATGGAGCTGCGCAAAG ACTATAGTATGTGTAGCATGCTGTAAAAGGCCACAAACACCAACAGTAAGTAATAACCAAATAG ACAGCATAGATGTACTCAATTATTTAAGTGAAATTTCATCATACATTAGCAGCTCCACCAATCC AGTACTTCTGGGCTGATTCACAAGGAGTATTTATCACAAACTCTCTTGTTTTAATATCATAAGT AGCCACTGTCTCAATTCCTCGGACCTTCAAATTAGTCAAATAAGGCAAAGAAAAATCTCATCAA CACAGCTAACAAAACTACAATTCAGTAATTTGACCAAAATATCATCACATACATTGCTCCCATG ACCCAGTTCTGTCATGGAAAAACAACCCTTGATAACATAATTTTCTGTGTCCGACAACCATTTG TCATGGTGGCGCTTTGTTCCAAGAAACTTGATTGCGCTGCCC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_006711* osi_circ_016164 						
					
						PMCS
						0.394854469

Conservation Information
Conserved circRNAs	osi_circ_006711* osi_circ_016164
PMCS	0.394854469

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							14508887-14508076(+)
14508905-14508955(-)
14508258-14508955(-)
						
						Potential amino acid sequence
						
							MTQFCHGKTTLDNIIFCVRQPFVMVALCSKKLDCAAPLKYKPH*(+)
MTELGHGSNVRGIETVATYDIKTREFVINTPCESAQKYWIGGAANHATHTIVFAQLHINGRNEG
VHAFVAQIRDEHENVMPNIQIADCGHKIGLNGVDNGRIWFNNIRVPRENLLNLVADVLPDGQYV
STIDDPDQRFAAFLSPLTLGRVNIAVNAVYISKGQRNQVSWNKAPP*(-)
MTLIRGLQHFYLHLHLVELILQLMRFIFQRGSAIKFLGTKRHHDKWLSDTENYVIKGCFSMTEL
GHGSNVRGIETVATYDIKTREFVINTPCESAQKYWIGGAANHATHTIVFAQLHINGRNEGVHAF
VAQIRDEHENVMPNIQIADCGHKIGLNGVDNGRIWFNNIRVPRENLLNLVADVLPDGQYVSTID
DPDQRFAAFLSPLTLGRVNIAVNAVYISKGQRNQVSWNKAPP*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	14508887-14508076(+) 14508905-14508955(-) 14508258-14508955(-)
Potential amino acid sequence	MTQFCHGKTTLDNIIFCVRQPFVMVALCSKKLDCAAPLKYKPH(+) MTELGHGSNVRGIETVATYDIKTREFVINTPCESAQKYWIGGAANHATHTIVFAQLHINGRNEG VHAFVAQIRDEHENVMPNIQIADCGHKIGLNGVDNGRIWFNNIRVPRENLLNLVADVLPDGQYV STIDDPDQRFAAFLSPLTLGRVNIAVNAVYISKGQRNQVSWNKAPP(-) MTLIRGLQHFYLHLHLVELILQLMRFIFQRGSAIKFLGTKRHHDKWLSDTENYVIKGCFSMTEL GHGSNVRGIETVATYDIKTREFVINTPCESAQKYWIGGAANHATHTIVFAQLHINGRNEGVHAF VAQIRDEHENVMPNIQIADCGHKIGLNGVDNGRIWFNNIRVPRENLLNLVADVLPDGQYVSTID DPDQRFAAFLSPLTLGRVNIAVNAVYISKGQRNQVSWNKAPP*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os06g0354500_circ_g.2

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