Plant Circular RNA Database

Detail information of Os03g0138100_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os03g0138100_circ_g.4
					
						ID in PlantcircBase
						osa_circ_017844
					
						Alias
						Os_ciR8646
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 2104811-2105800  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os03g0138100						
					
						Parent gene annotation
						
							Similar to RNA recognition motif family protein, expressed. (Os0
3t0138100-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os03g0138100_circ_g.1 Os03g0138100_circ_g.2 Os03g0138100_circ_g.3 Os03g0138100_circ_g.5 Os03g0138100_circ_g.6 Os03g0138100_circ_g.7 						
					
						Support reads
						2/1
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0138100-01:5

General Information
CircRNA Name	Os03g0138100_circ_g.4
ID in PlantcircBase	osa_circ_017844
Alias	Os_ciR8646
Organism	Oryza sativa
Position	chr3: 2104811-2105800 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os03g0138100
Parent gene annotation	Similar to RNA recognition motif family protein, expressed. (Os0 3t0138100-01)
Parent gene strand	-
Alternative splicing	Os03g0138100_circ_g.1 Os03g0138100_circ_g.2 Os03g0138100_circ_g.3 Os03g0138100_circ_g.5 Os03g0138100_circ_g.6 Os03g0138100_circ_g.7
Support reads	2/1
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0138100-01:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TGAGAATGGACTGGCTCTGGCAAGTTACCTTGTGTGGTGAATCTGCAACAACATCGGATATCAA
TTTAATCTCTTCAGAAGGCTTCTTAGGAGCAACATGcacattttttagctgcaggacttttgtt
ggttcttcaagtagtgatttggcactgtcaggaattccataaaatggggaagcttcattaaaag
cctgaaat

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 405
Transcript exons: 2104811-2104903,2104987-2105094,2105225-2105314,2105447-
2105488,2105729-2105800
CACATTTTTTAGCTGCAGGACTTTTGTTGGTTCTTCAAGTAGTGATTTGGCACTGTCAGGAATT
CCATAAAATGGGGAAGCTTCATTAAAAGCCTGCTCGGTAGAGTTAGGGAACACATTAACAGCAG
TTAGGATGCCTCCACCAAGCTTCATCCCATTGAGGCCAGCACATGCCTTCGATGTAATGGAGTG
ATCAATGTACTCAAGAAATGCACATGCCCCACCAAGGTATTCATTAAAAAGAAAGCGGTAAGCA
GCCAACGGACCAAATGAACTAACAATTTCCATGAGCATCTCAGATGAAATCACTCCAGAAATCC
CAGCGATGAAAATCTTGTGTGGTGAATCTGCAACAACATCGGATATCAATTTAATCTCTTCAGA
AGGCTTCTTAGGAGCAACATG
						
						Genomic sequence
						
							CACATTTTTTAGCTGCAGGACTTTTGTTGGTTCTTCAAGTAGTGATTTGGCACTGTCAGGAATT
CCATAAAATGGGGAAGCTTCATTAAAAGCCTGAAATTTCAAAGGAAACAGAGTGGGACTTAATT
CAATGAAATGGAAATGACAGAAAATGAATGAATCTAAAATGTTTGAACCTGCTCGGTAGAGTTA
GGGAACACATTAACAGCAGTTAGGATGCCTCCACCAAGCTTCATCCCATTGAGGCCAGCACATG
CCTTCGATGTAATGGAGTGATCAATGTACTGCACACATCAAATTGATAAGAAATCAGCTTAAGT
AATATATTCATCTGGTCTAGTTTTTTTTTTATCTTCTCCATCAATGACATGTTTGAAAAAAAAC
GATTTAGTTTAGTACCAGAGCTTTCCATACCTCAAGAAATGCACATGCCCCACCAAGGTATTCA
TTAAAAAGAAAGCGGTAAGCAGCCAACGGACCAAATGAACTAACAATTTCCATGAGCTGAAAGA
GGACAATGTAGAAGCTTCTGAGGGATAACCGGATAACAGCTAATAACCACAATACCACATGCAG
AGCATGATCACTGGATTAAAACAAGTTATGCAAATATACTGGTGGATGCTTTTGTATTACCATC
TCAGATGAAATCACTCCAGAAATCCCAGCGATGAAAATCTGCAAAAGTTAAAAAAGTTGAGACA
TCCGATGAAAGCTACTAAAGTTCCTAGATAATCAAACCTATATGTTTTATATTAACTTCAATTA
ACAGCGGATGTACGATATCCATGTATCAAATATTTGGCCAGAAAAGTAAGAATTTCTATCAATC
AGAAAATGCAAATATTTTTTCACCGCCTCTGGAAATATAGTCATCCATATCATAGATATGAGAA
TGGACTGGCTCTGGCAAGTTACCTTGTGTGGTGAATCTGCAACAACATCGGATATCAATTTAAT
CTCTTCAGAAGGCTTCTTAGGAGCAACATG

Sequences
Splice junction sequence	TGAGAATGGACTGGCTCTGGCAAGTTACCTTGTGTGGTGAATCTGCAACAACATCGGATATCAA TTTAATCTCTTCAGAAGGCTTCTTAGGAGCAACATGcacattttttagctgcaggacttttgtt ggttcttcaagtagtgatttggcactgtcaggaattccataaaatggggaagcttcattaaaag cctgaaat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 405 Transcript exons: 2104811-2104903,2104987-2105094,2105225-2105314,2105447- 2105488,2105729-2105800 CACATTTTTTAGCTGCAGGACTTTTGTTGGTTCTTCAAGTAGTGATTTGGCACTGTCAGGAATT CCATAAAATGGGGAAGCTTCATTAAAAGCCTGCTCGGTAGAGTTAGGGAACACATTAACAGCAG TTAGGATGCCTCCACCAAGCTTCATCCCATTGAGGCCAGCACATGCCTTCGATGTAATGGAGTG ATCAATGTACTCAAGAAATGCACATGCCCCACCAAGGTATTCATTAAAAAGAAAGCGGTAAGCA GCCAACGGACCAAATGAACTAACAATTTCCATGAGCATCTCAGATGAAATCACTCCAGAAATCC CAGCGATGAAAATCTTGTGTGGTGAATCTGCAACAACATCGGATATCAATTTAATCTCTTCAGA AGGCTTCTTAGGAGCAACATG
Genomic sequence	CACATTTTTTAGCTGCAGGACTTTTGTTGGTTCTTCAAGTAGTGATTTGGCACTGTCAGGAATT CCATAAAATGGGGAAGCTTCATTAAAAGCCTGAAATTTCAAAGGAAACAGAGTGGGACTTAATT CAATGAAATGGAAATGACAGAAAATGAATGAATCTAAAATGTTTGAACCTGCTCGGTAGAGTTA GGGAACACATTAACAGCAGTTAGGATGCCTCCACCAAGCTTCATCCCATTGAGGCCAGCACATG CCTTCGATGTAATGGAGTGATCAATGTACTGCACACATCAAATTGATAAGAAATCAGCTTAAGT AATATATTCATCTGGTCTAGTTTTTTTTTTATCTTCTCCATCAATGACATGTTTGAAAAAAAAC GATTTAGTTTAGTACCAGAGCTTTCCATACCTCAAGAAATGCACATGCCCCACCAAGGTATTCA TTAAAAAGAAAGCGGTAAGCAGCCAACGGACCAAATGAACTAACAATTTCCATGAGCTGAAAGA GGACAATGTAGAAGCTTCTGAGGGATAACCGGATAACAGCTAATAACCACAATACCACATGCAG AGCATGATCACTGGATTAAAACAAGTTATGCAAATATACTGGTGGATGCTTTTGTATTACCATC TCAGATGAAATCACTCCAGAAATCCCAGCGATGAAAATCTGCAAAAGTTAAAAAAGTTGAGACA TCCGATGAAAGCTACTAAAGTTCCTAGATAATCAAACCTATATGTTTTATATTAACTTCAATTA ACAGCGGATGTACGATATCCATGTATCAAATATTTGGCCAGAAAAGTAAGAATTTCTATCAATC AGAAAATGCAAATATTTTTTCACCGCCTCTGGAAATATAGTCATCCATATCATAGATATGAGAA TGGACTGGCTCTGGCAAGTTACCTTGTGTGGTGAATCTGCAACAACATCGGATATCAATTTAAT CTCTTCAGAAGGCTTCTTAGGAGCAACATG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_013243 						
					
						PMCS
						0.261918384

Conservation Information
Conserved circRNAs	osi_circ_013243
PMCS	0.261918384

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							2105311-2104877(+)
2105451-2104813(-)
						
						Potential amino acid sequence
						
							MSISDEITPEIPAMKILCGESATTSDINLISSEGFLGATCTFFSCRTFVGSSSSDLALSGIP*(
+)
MLMEIVSSFGPLAAYRFLFNEYLGGACAFLEYIDHSITSKACAGLNGMKLGGGILTAVNVFPNS
TEQAFNEASPFYGIPDSAKSLLEEPTKVLQLKNVHVAPKKPSEEIKLISDVVADSPHKIFIAGI
SGVISSEMLMEIVSSFGPLAAYRFLFNEYLGGACAFLEYIDHSITSKACAGLNGMKLGGGILTA
VNVFPNSTEQAFNEASPFYGIPDSAKSLLEEPTKVLQLKNVHVAPKKPSEEIKLISDVVADSPH
KIFIAGISGVISSEMLMEIVSSFGPLAAYRFLFNEYLGGACAFLEYIDHSITSKACAGLNGMKL
GGGILTAVNVFPNSTEQAFNEASPFYGIPDSAKSLLEEPTKVLQLKNVHVAPKKPSEEIKLISD
VVADSPHKIFIAGISGVISSEMLMEIVSSFGPLAAYRFLFNEYLGGACAFLEYIDHSITSKACA
GLNGMKLGGGILTAVNVFPNSTEQAFNEASPFYGIPDSAKSLLEEPTKVLQLKNV(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	2105311-2104877(+) 2105451-2104813(-)
Potential amino acid sequence	MSISDEITPEIPAMKILCGESATTSDINLISSEGFLGATCTFFSCRTFVGSSSSDLALSGIP*( +) MLMEIVSSFGPLAAYRFLFNEYLGGACAFLEYIDHSITSKACAGLNGMKLGGGILTAVNVFPNS TEQAFNEASPFYGIPDSAKSLLEEPTKVLQLKNVHVAPKKPSEEIKLISDVVADSPHKIFIAGI SGVISSEMLMEIVSSFGPLAAYRFLFNEYLGGACAFLEYIDHSITSKACAGLNGMKLGGGILTA VNVFPNSTEQAFNEASPFYGIPDSAKSLLEEPTKVLQLKNVHVAPKKPSEEIKLISDVVADSPH KIFIAGISGVISSEMLMEIVSSFGPLAAYRFLFNEYLGGACAFLEYIDHSITSKACAGLNGMKL GGGILTAVNVFPNSTEQAFNEASPFYGIPDSAKSLLEEPTKVLQLKNVHVAPKKPSEEIKLISD VVADSPHKIFIAGISGVISSEMLMEIVSSFGPLAAYRFLFNEYLGGACAFLEYIDHSITSKACA GLNGMKLGGGILTAVNVFPNSTEQAFNEASPFYGIPDSAKSLLEEPTKVLQLKNV(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os03g0138100_circ_g.4

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