Plant Circular RNA Database

Detail information of Zm00001d039041_circ_g.2

			
						 General Information
					
						CircRNA Name
						Zm00001d039041_circ_g.2
					
						ID in PlantcircBase
						zma_circ_009178
					
						Alias
						zma_circ_0002428, GRMZM2G101515_C1
					
						Organism
						
							Zea mays						
					
						Position
						
							chr6: 169134174-169134811  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ, CIRI2
					
						Parent gene
						
							Zm00001d039041						
					
						Parent gene annotation
						
							Putative DUF1296 domain containing family protein  
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Zm00001d039041_circ_g.1 Zm00001d039041_circ_g.3 						
					
						Support reads
						NA
					
						Tissues
						leaf, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d039041_T004:2
Zm00001d039041_T008:2
Zm00001d039041_T002:2
Zm00001d039041_T009:2
Zm00001d039041_T007:2
Zm00001d039041_T005:2
Zm00001d039041_T003:2
Zm00001d039041_T006:2

General Information
CircRNA Name	Zm00001d039041_circ_g.2
ID in PlantcircBase	zma_circ_009178
Alias	zma_circ_0002428, GRMZM2G101515_C1
Organism	Zea mays
Position	chr6: 169134174-169134811 JBrowse»
Reference genome	AGPv4.38
Type	e-circRNA
Identification method	find_circ, CIRI2
Parent gene	Zm00001d039041
Parent gene annotation	Putative DUF1296 domain containing family protein
Parent gene strand	+
Alternative splicing	Zm00001d039041_circ_g.1 Zm00001d039041_circ_g.3
Support reads	NA
Tissues	leaf, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d039041_T004:2 Zm00001d039041_T008:2 Zm00001d039041_T002:2 Zm00001d039041_T009:2 Zm00001d039041_T007:2 Zm00001d039041_T005:2 Zm00001d039041_T003:2 Zm00001d039041_T006:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCATGCTTATTCAAACACGGACACTTTACAGCCTAGCACAATTGAGGAGCCACAAGGAAACAAC
CAAGCACATACTCTTTCCCATTTGTCAAACTTAATGttgaggattccagtgctgatgtatcagc
agcagcagcaaacttccagagtttaagcctacataatgatgagttagctgcaaaaaagtctgca
gaagataa

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 554
Transcript exons: 169134174-169134450,169134535-169134811
TTGAGGATTCCAGTGCTGATGTATCAGCAGCAGCAGCAAACTTCCAGAGTTTAAGCCTACATAA
TGATGAGTTAGCTGCAAAAAAGTCTGCAGAAGATAACCCTGCAGTGATAATTCCTGACCACTTG
CAAGTTACAAATACAGAATGTGTCAAGTTGAGCTTTGGCAGTTTTGGATCTGGTGCATTTTCTG
GGTTACTCCCACAAAATACCACTGATAGCAATGTGGAATTCCCTGCTCGAGAGGAATCTGCACC
AGTAGATCAAATAGATGCCAGGAATCAAGATTACTATGAAAGTGGTGCTGTAACTTCACCAGCA
GACGAAAATCTTGAGGCCATGCTTGGAGCTAACATGGAGAATGTAGATGCGCCTTCTGTTTCAC
AGGCTAATGAGCTCAGGCAGGACGTACTTGATCCTTCTGAACTTCAATATGACGTCCCATCAGT
TCCAAGTCATGCTTATTCAAACACGGACACTTTACAGCCTAGCACAATTGAGGAGCCACAAGGA
AACAACCAAGCACATACTCTTTCCCATTTGTCAAACTTAATG
						
						Genomic sequence
						
							TTGAGGATTCCAGTGCTGATGTATCAGCAGCAGCAGCAAACTTCCAGAGTTTAAGCCTACATAA
TGATGAGTTAGCTGCAAAAAAGTCTGCAGAAGATAACCCTGCAGTGATAATTCCTGACCACTTG
CAAGTTACAAATACAGAATGTGTCAAGTTGAGCTTTGGCAGTTTTGGATCTGGTGCATTTTCTG
GGTTACTCCCACAAAATACCACTGATAGCAATGTGGAATTCCCTGCTCGAGAGGAATCTGCACC
AGTAGATCAAATAGATGCCAGGTGAAGCATTTTACTGGTGTTTTTTTGGCACTTCCCTTACCGC
TCTTTGCGGGGAAGTTAACATTGTGGAATTGTCTGTTGCAGGAATCAAGATTACTATGAAAGTG
GTGCTGTAACTTCACCAGCAGACGAAAATCTTGAGGCCATGCTTGGAGCTAACATGGAGAATGT
AGATGCGCCTTCTGTTTCACAGGCTAATGAGCTCAGGCAGGACGTACTTGATCCTTCTGAACTT
CAATATGACGTCCCATCAGTTCCAAGTCATGCTTATTCAAACACGGACACTTTACAGCCTAGCA
CAATTGAGGAGCCACAAGGAAACAACCAAGCACATACTCTTTCCCATTTGTCAAACTTAATG

Sequences
Splice junction sequence	TCATGCTTATTCAAACACGGACACTTTACAGCCTAGCACAATTGAGGAGCCACAAGGAAACAAC CAAGCACATACTCTTTCCCATTTGTCAAACTTAATGttgaggattccagtgctgatgtatcagc agcagcagcaaacttccagagtttaagcctacataatgatgagttagctgcaaaaaagtctgca gaagataa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 554 Transcript exons: 169134174-169134450,169134535-169134811 TTGAGGATTCCAGTGCTGATGTATCAGCAGCAGCAGCAAACTTCCAGAGTTTAAGCCTACATAA TGATGAGTTAGCTGCAAAAAAGTCTGCAGAAGATAACCCTGCAGTGATAATTCCTGACCACTTG CAAGTTACAAATACAGAATGTGTCAAGTTGAGCTTTGGCAGTTTTGGATCTGGTGCATTTTCTG GGTTACTCCCACAAAATACCACTGATAGCAATGTGGAATTCCCTGCTCGAGAGGAATCTGCACC AGTAGATCAAATAGATGCCAGGAATCAAGATTACTATGAAAGTGGTGCTGTAACTTCACCAGCA GACGAAAATCTTGAGGCCATGCTTGGAGCTAACATGGAGAATGTAGATGCGCCTTCTGTTTCAC AGGCTAATGAGCTCAGGCAGGACGTACTTGATCCTTCTGAACTTCAATATGACGTCCCATCAGT TCCAAGTCATGCTTATTCAAACACGGACACTTTACAGCCTAGCACAATTGAGGAGCCACAAGGA AACAACCAAGCACATACTCTTTCCCATTTGTCAAACTTAATG
Genomic sequence	TTGAGGATTCCAGTGCTGATGTATCAGCAGCAGCAGCAAACTTCCAGAGTTTAAGCCTACATAA TGATGAGTTAGCTGCAAAAAAGTCTGCAGAAGATAACCCTGCAGTGATAATTCCTGACCACTTG CAAGTTACAAATACAGAATGTGTCAAGTTGAGCTTTGGCAGTTTTGGATCTGGTGCATTTTCTG GGTTACTCCCACAAAATACCACTGATAGCAATGTGGAATTCCCTGCTCGAGAGGAATCTGCACC AGTAGATCAAATAGATGCCAGGTGAAGCATTTTACTGGTGTTTTTTTGGCACTTCCCTTACCGC TCTTTGCGGGGAAGTTAACATTGTGGAATTGTCTGTTGCAGGAATCAAGATTACTATGAAAGTG GTGCTGTAACTTCACCAGCAGACGAAAATCTTGAGGCCATGCTTGGAGCTAACATGGAGAATGT AGATGCGCCTTCTGTTTCACAGGCTAATGAGCTCAGGCAGGACGTACTTGATCCTTCTGAACTT CAATATGACGTCCCATCAGTTCCAAGTCATGCTTATTCAAACACGGACACTTTACAGCCTAGCA CAATTGAGGAGCCACAAGGAAACAACCAAGCACATACTCTTTCCCATTTGTCAAACTTAATG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.197313354

Conservation Information
Conserved circRNAs	NA
PMCS	0.197313354

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							169134597-169134224(+)
						
						Potential amino acid sequence
						
							MLGANMENVDAPSVSQANELRQDVLDPSELQYDVPSVPSHAYSNTDTLQPSTIEEPQGNNQAHT
LSHLSNLMLRIPVLMYQQQQQTSRV*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	169134597-169134224(+)
Potential amino acid sequence	MLGANMENVDAPSVSQANELRQDVLDPSELQYDVPSVPSHAYSNTDTLQPSTIEEPQGNNQAHT LSHLSNLMLRIPVLMYQQQQQTSRV*(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ma et al., 2021b;Zhang et al., 2019

circRNA

Detail information of Zm00001d039041_circ_g.2

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