Plant Circular RNA Database

Detail information of Os05g0592400_circ_g.6

			
						 General Information
					
						CircRNA Name
						Os05g0592400_circ_g.6
					
						ID in PlantcircBase
						osa_circ_029171
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 29515814-29516313  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os05g0592400						
					
						Parent gene annotation
						
							UV-damaged DNA binding protein. (Os05t0592400-01);Similar to UV-
damaged DNA binding protein. (Os05t0592400-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os05g0592400_circ_g.5 Os05g0592400_circ_g.7 						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0592400-01:2

General Information
CircRNA Name	Os05g0592400_circ_g.6
ID in PlantcircBase	osa_circ_029171
Alias	NA
Organism	Oryza sativa
Position	chr5: 29515814-29516313 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os05g0592400
Parent gene annotation	UV-damaged DNA binding protein. (Os05t0592400-01);Similar to UV- damaged DNA binding protein. (Os05t0592400-02)
Parent gene strand	+
Alternative splicing	Os05g0592400_circ_g.5 Os05g0592400_circ_g.7
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0592400-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GAACATGGAAGATGAGTTAGAAGAAACTGAGATAGAAGGCTTTGATGCGCAGACTCAGACTTTG
TTTTGTCAAAATGCCATCAATGATCTGCTTATACAGcttgtaaaactaaatctccaagctgacc
caaatggttcatatgttgaagttcttgagcgatatgtgaaccttggacccatcgtggacttctg
tgtggttg

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 417
Transcript exons: 29515814-29516014,29516098-29516313
CTTGTAAAACTAAATCTCCAAGCTGACCCAAATGGTTCATATGTTGAAGTTCTTGAGCGATATG
TGAACCTTGGACCCATCGTGGACTTCTGTGTGGTTGACCTTGACAGGCAGGGTCAGGGTCAGGT
TGTCACTTGTTCAGGAGCATTTAAAGATGGTTCCCTTCGGGTGGTTCGGAATGGGATAGGTATC
AATGAGCAGGCTTCAGTAGAACTACAAGGGATCAAGGGTTTATGGTCGTTGAAATCTTCCTTCA
ATGATCCGTATGACATGTACCTAGTTGTGAGCTTTATAAGCGAGACAAGATTCTTGGCTATGAA
CATGGAAGATGAGTTAGAAGAAACTGAGATAGAAGGCTTTGATGCGCAGACTCAGACTTTGTTT
TGTCAAAATGCCATCAATGATCTGCTTATACAG
						
						Genomic sequence
						
							CTTGTAAAACTAAATCTCCAAGCTGACCCAAATGGTTCATATGTTGAAGTTCTTGAGCGATATG
TGAACCTTGGACCCATCGTGGACTTCTGTGTGGTTGACCTTGACAGGCAGGGTCAGGGTCAGGT
TGTCACTTGTTCAGGAGCATTTAAAGATGGTTCCCTTCGGGTGGTTCGGAATGGGATAGGTATC
AATGAGCAGGTATTGAATTAAGATCTATCCAATATTGTCCAATTTTAGTTCTGCATAAAGTTGA
GCTATATTATCTCTTATCATGTAATTAGGCTTCAGTAGAACTACAAGGGATCAAGGGTTTATGG
TCGTTGAAATCTTCCTTCAATGATCCGTATGACATGTACCTAGTTGTGAGCTTTATAAGCGAGA
CAAGATTCTTGGCTATGAACATGGAAGATGAGTTAGAAGAAACTGAGATAGAAGGCTTTGATGC
GCAGACTCAGACTTTGTTTTGTCAAAATGCCATCAATGATCTGCTTATACAG

Sequences
Splice junction sequence	GAACATGGAAGATGAGTTAGAAGAAACTGAGATAGAAGGCTTTGATGCGCAGACTCAGACTTTG TTTTGTCAAAATGCCATCAATGATCTGCTTATACAGcttgtaaaactaaatctccaagctgacc caaatggttcatatgttgaagttcttgagcgatatgtgaaccttggacccatcgtggacttctg tgtggttg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 417 Transcript exons: 29515814-29516014,29516098-29516313 CTTGTAAAACTAAATCTCCAAGCTGACCCAAATGGTTCATATGTTGAAGTTCTTGAGCGATATG TGAACCTTGGACCCATCGTGGACTTCTGTGTGGTTGACCTTGACAGGCAGGGTCAGGGTCAGGT TGTCACTTGTTCAGGAGCATTTAAAGATGGTTCCCTTCGGGTGGTTCGGAATGGGATAGGTATC AATGAGCAGGCTTCAGTAGAACTACAAGGGATCAAGGGTTTATGGTCGTTGAAATCTTCCTTCA ATGATCCGTATGACATGTACCTAGTTGTGAGCTTTATAAGCGAGACAAGATTCTTGGCTATGAA CATGGAAGATGAGTTAGAAGAAACTGAGATAGAAGGCTTTGATGCGCAGACTCAGACTTTGTTT TGTCAAAATGCCATCAATGATCTGCTTATACAG
Genomic sequence	CTTGTAAAACTAAATCTCCAAGCTGACCCAAATGGTTCATATGTTGAAGTTCTTGAGCGATATG TGAACCTTGGACCCATCGTGGACTTCTGTGTGGTTGACCTTGACAGGCAGGGTCAGGGTCAGGT TGTCACTTGTTCAGGAGCATTTAAAGATGGTTCCCTTCGGGTGGTTCGGAATGGGATAGGTATC AATGAGCAGGTATTGAATTAAGATCTATCCAATATTGTCCAATTTTAGTTCTGCATAAAGTTGA GCTATATTATCTCTTATCATGTAATTAGGCTTCAGTAGAACTACAAGGGATCAAGGGTTTATGG TCGTTGAAATCTTCCTTCAATGATCCGTATGACATGTACCTAGTTGTGAGCTTTATAAGCGAGA CAAGATTCTTGGCTATGAACATGGAAGATGAGTTAGAAGAAACTGAGATAGAAGGCTTTGATGC GCAGACTCAGACTTTGTTTTGTCAAAATGCCATCAATGATCTGCTTATACAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.412265917

Conservation Information
Conserved circRNAs	NA
PMCS	0.412265917

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							29516168-29516310(+)
29516259-29515817(+)
29515895-29516285(-)
						
						Potential amino acid sequence
						
							MYLVVSFISETRFLAMNMEDELEETEIEGFDAQTQTLFCQNAINDLLIQLVKLNLQADPNGSYV
EVLERYVNLGPIVDFCVVDLDRQGQGQVVTCSGAFKDGSLRVVRNGIGINEQASVELQGIKGLW
SLKSSFNDPYDMYLVVSFISETRFLAMNMEDELEETEIEGFDAQTQTLFCQNAINDLLIQLVKL
NLQADPNGSYVEVLERYVNLGPIVDFCVVDLDRQGQGQVVTCSGAFKDGSLRVVRNGIGINEQA
SVELQGIKGLWSLKSSFNDPYDMYLVVSFISETRFLAMNMEDELEETEIEGFDAQTQTLFCQNA
INDLLIQLVKLNLQADPNGSYVEVLERYVNLGPIVDFCVVDLDRQGQGQVVTCSGAFKDGSLRV
VRNGIGINEQASVELQGIKGLWSLKSSFNDPYDMYLVVSFISETRFLAMNMEDELEETEIEGFD
AQTQTLFCQNAINDLLIQ(+)
MRRLRLCFVKMPSMICLYSL*(+)
MGPRFTYRSRTSTYEPFGSAWRFSFTSCISRSLMAF*(-)
						
						Sponge-miRNAs
						
							osa-miR169n 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	29516168-29516310(+) 29516259-29515817(+) 29515895-29516285(-)
Potential amino acid sequence	MYLVVSFISETRFLAMNMEDELEETEIEGFDAQTQTLFCQNAINDLLIQLVKLNLQADPNGSYV EVLERYVNLGPIVDFCVVDLDRQGQGQVVTCSGAFKDGSLRVVRNGIGINEQASVELQGIKGLW SLKSSFNDPYDMYLVVSFISETRFLAMNMEDELEETEIEGFDAQTQTLFCQNAINDLLIQLVKL NLQADPNGSYVEVLERYVNLGPIVDFCVVDLDRQGQGQVVTCSGAFKDGSLRVVRNGIGINEQA SVELQGIKGLWSLKSSFNDPYDMYLVVSFISETRFLAMNMEDELEETEIEGFDAQTQTLFCQNA INDLLIQLVKLNLQADPNGSYVEVLERYVNLGPIVDFCVVDLDRQGQGQVVTCSGAFKDGSLRV VRNGIGINEQASVELQGIKGLWSLKSSFNDPYDMYLVVSFISETRFLAMNMEDELEETEIEGFD AQTQTLFCQNAINDLLIQ(+) MRRLRLCFVKMPSMICLYSL(+) MGPRFTYRSRTSTYEPFGSAWRFSFTSCISRSLMAF(-)
Sponge-miRNAs	osa-miR169n
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os05g0592400_circ_g.6

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