Plant Circular RNA Database

Detail information of Csa1G652810_circ_g.2

			
						 General Information
					
						CircRNA Name
						Csa1G652810_circ_g.2
					
						ID in PlantcircBase
						csa_circ_000671
					
						Alias
						Chr1:26028974_26030468
					
						Organism
						
							Cucumis sativus						
					
						Position
						
							chrChr1: 26028974-26030468  JBrowse»						
					
						Reference genome
						
							Cucumis sativus v1.5						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI2;Find_circ
					
						Parent gene
						
							Csa1G652810						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						-
					
						Alternative splicing
						
							Csa1G652810_circ_g.1 						
					
						Support reads
						NA
					
						Tissues
						leaf,root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Csa1G652810.1:4

General Information
CircRNA Name	Csa1G652810_circ_g.2
ID in PlantcircBase	csa_circ_000671
Alias	Chr1:26028974_26030468
Organism	Cucumis sativus
Position	chrChr1: 26028974-26030468 JBrowse»
Reference genome	Cucumis sativus v1.5
Type	e-circRNA
Identification method	CIRI2;Find_circ
Parent gene	Csa1G652810
Parent gene annotation	NA
Parent gene strand	-
Alternative splicing	Csa1G652810_circ_g.1
Support reads	NA
Tissues	leaf,root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Csa1G652810.1:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AGCATACCTTTACTGATAGGTTAACCTCATGTTTGGGTTTAATGTCAACTTGGATACTGCAGGA
AGGAGCATCGGTCTCAACACCTTTCTCACAAAGAGGcaagctattttgttgtgaaggtgtacgc
caagggacgtggactgtaatgccattgacagtaatgtcccacatcactttcttatatcctgaca
ttatgcat

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							CAAGCTATTTTGTTGTGAAGGTGTACGCCAAGGGACGTGGACTGTAATGCCATTGACAGTAATG
TCCCACATCACTTTCTTATATCCTGACATTATGCATCTGGAGGATCAGAAACAACAAACAATGA
CAGTGCGCACATGGAGTAAATAAGATGGAATAGATATAGACTACATTAATGCCTGAATTACCAA
GTCATGCAACAATTATACAAGTTAAACTTACTGAGCCTTGGATAGCAATCGAGCTTTGGAATTT
TCAATTCCATTTAGAGTTGAAAGAACCTACAAATAAAAATGCTTTTTAATCAATTTACAAGCCC
ATTGAGTGGATATATGAAAACTAACATCACAGCTACATGGATCGGTGCTTTTAACATCTCTCAC
AGAAATTGTTCATACATTCAATACATCTACGTCTTTCTCCTGATTATAACTATAGTCTACAGAC
CTTCATGAGTCTTGACACATTGGAGATGTTTTTGTAATAACTCAAAGTTAGAATTGTTTTGAAA
TCTATCAATGAAGTGGTCTCAGTTTCCACTCTAAAAATAGTAATATCATATTAATGATAATTAA
TTAATTAAAAGAAAACCCTCCAGTGAGAAGAAAAACTAGTCCAAAACAACAGTTCAAAGAAAAA
GAAAGACTACTCCACATCCTATCAAAGCGACTAGAAAAAACCCTAATGCTGTATCTTGAGATTT
ATTCTCTTCTCATTGATTTGAAAGTTCTGTTTACATTTCAAAAAAATAAAAAATAAACTAGTCA
GACAGAGAGGGCTTAAAAATATCTAAAAGAATAATTTAGAGCTACCATGTTGTTAAAAATTTGA
AATCATCAATTAATAGTAGATTTCTTTTGAATATTGAATAACACAACCAACTGCTAACAGCTCT
AAACAATCAATTAATAGTATTAGACATAAAATGGACATATGTAGGCCTAAGTCACATATTCCTA
CTAACAGCTCTAAACAAACTTGTCACAGAAATAAAAAATCATTTTAACTGATGCAATAGGATTA
AGTTAAATTGGGGTACCTTGGTCAAATTCTTGATATAATAAAAATACTAATGCTCAAAAGATAT
AAACTCCATAAAAGGAGGGAGATAAAGGCTAAAAACCCCAAATTAAAAGGAATCAAACTAAAGT
AAACAGCCTATAACAGAATAAACAATAGAAGAAAGCAGCCTTACCCTTCTGTGAAGAGATTCGA
AGGACTTAAGTTCATCAAAAAACTCCGACAGACAAAGAAAAAACTTGGAATCATAAGTAACTAC
TAATGGATTCAGCATCACCTGCAAATAATATACAGGAAAAATTGCTGAATAGTAGCAGACTTCA
AATAATGAAAAAAATGGTTCTCCGATCCAGCTTCAAATAGGCACATCAATTAGCATACCTTTAC
TGATAGGTTAACCTCATGTTTGGGTTTAATGTCAACTTGGATACTGCAGGAAGGAGCATCGGTC
TCAACACCTTTCTCACAAAGAGG
						
					

			
		

Sequences
Splice junction sequence	AGCATACCTTTACTGATAGGTTAACCTCATGTTTGGGTTTAATGTCAACTTGGATACTGCAGGA AGGAGCATCGGTCTCAACACCTTTCTCACAAAGAGGcaagctattttgttgtgaaggtgtacgc caagggacgtggactgtaatgccattgacagtaatgtcccacatcactttcttatatcctgaca ttatgcat
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CAAGCTATTTTGTTGTGAAGGTGTACGCCAAGGGACGTGGACTGTAATGCCATTGACAGTAATG TCCCACATCACTTTCTTATATCCTGACATTATGCATCTGGAGGATCAGAAACAACAAACAATGA CAGTGCGCACATGGAGTAAATAAGATGGAATAGATATAGACTACATTAATGCCTGAATTACCAA GTCATGCAACAATTATACAAGTTAAACTTACTGAGCCTTGGATAGCAATCGAGCTTTGGAATTT TCAATTCCATTTAGAGTTGAAAGAACCTACAAATAAAAATGCTTTTTAATCAATTTACAAGCCC ATTGAGTGGATATATGAAAACTAACATCACAGCTACATGGATCGGTGCTTTTAACATCTCTCAC AGAAATTGTTCATACATTCAATACATCTACGTCTTTCTCCTGATTATAACTATAGTCTACAGAC CTTCATGAGTCTTGACACATTGGAGATGTTTTTGTAATAACTCAAAGTTAGAATTGTTTTGAAA TCTATCAATGAAGTGGTCTCAGTTTCCACTCTAAAAATAGTAATATCATATTAATGATAATTAA TTAATTAAAAGAAAACCCTCCAGTGAGAAGAAAAACTAGTCCAAAACAACAGTTCAAAGAAAAA GAAAGACTACTCCACATCCTATCAAAGCGACTAGAAAAAACCCTAATGCTGTATCTTGAGATTT ATTCTCTTCTCATTGATTTGAAAGTTCTGTTTACATTTCAAAAAAATAAAAAATAAACTAGTCA GACAGAGAGGGCTTAAAAATATCTAAAAGAATAATTTAGAGCTACCATGTTGTTAAAAATTTGA AATCATCAATTAATAGTAGATTTCTTTTGAATATTGAATAACACAACCAACTGCTAACAGCTCT AAACAATCAATTAATAGTATTAGACATAAAATGGACATATGTAGGCCTAAGTCACATATTCCTA CTAACAGCTCTAAACAAACTTGTCACAGAAATAAAAAATCATTTTAACTGATGCAATAGGATTA AGTTAAATTGGGGTACCTTGGTCAAATTCTTGATATAATAAAAATACTAATGCTCAAAAGATAT AAACTCCATAAAAGGAGGGAGATAAAGGCTAAAAACCCCAAATTAAAAGGAATCAAACTAAAGT AAACAGCCTATAACAGAATAAACAATAGAAGAAAGCAGCCTTACCCTTCTGTGAAGAGATTCGA AGGACTTAAGTTCATCAAAAAACTCCGACAGACAAAGAAAAAACTTGGAATCATAAGTAACTAC TAATGGATTCAGCATCACCTGCAAATAATATACAGGAAAAATTGCTGAATAGTAGCAGACTTCA AATAATGAAAAAAATGGTTCTCCGATCCAGCTTCAAATAGGCACATCAATTAGCATACCTTTAC TGATAGGTTAACCTCATGTTTGGGTTTAATGTCAACTTGGATACTGCAGGAAGGAGCATCGGTC TCAACACCTTTCTCACAAAGAGG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.10035403

Conservation Information
Conserved circRNAs	NA
PMCS	0.10035403

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							26030270-26028976(-)
26029028-26030380(-)
						
						Potential amino acid sequence
						
							MLNPLVVTYDSKFFLCLSEFFDELKSFESLHRRVLSTLNGIENSKARLLSKAQCIMSGYKKVMW
DITVNGITVHVPWRTPSQQNSLPLCEKGVETDAPSCSIQVDIKPKHEVNLSVKVMLNPLVVTYD
SKFFLCLSEFFDELKSFESLHRRVLSTLNGIENSKARLLSKAQCIMSGYKKVMWDITVNGITVH
VPWRTPSQQNSLPLCEKGVETDAPSCSIQVDIKPKHEVNLSVKVMLNPLVVTYDSKFFLCLSEF
FDELKSFESLHRRVLSTLNGIENSKARLLSKAQCIMSGYKKVMWDITVNGITVHVPWRTPSQQN
SLPLCEKGVETDAPSCSIQVDIKPKHEVNLSVKVMLNPLVVTYDSKFFLCLSEFFDELKSFESL
HRRVLSTLNGIENSKARLLSKAQCIMSGYKKVMWDITVNGITVHVPWRTPSQQNSL
MALQSTSLGVHLHNKIACLFVRKVLRPMLLPAVSKLTLNPNMRLTYQ*
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to salt stress in root

Functional Information
Coding potential	Y
Potential coding position	26030270-26028976(-) 26029028-26030380(-)
Potential amino acid sequence	MLNPLVVTYDSKFFLCLSEFFDELKSFESLHRRVLSTLNGIENSKARLLSKAQCIMSGYKKVMW DITVNGITVHVPWRTPSQQNSLPLCEKGVETDAPSCSIQVDIKPKHEVNLSVKVMLNPLVVTYD SKFFLCLSEFFDELKSFESLHRRVLSTLNGIENSKARLLSKAQCIMSGYKKVMWDITVNGITVH VPWRTPSQQNSLPLCEKGVETDAPSCSIQVDIKPKHEVNLSVKVMLNPLVVTYDSKFFLCLSEF FDELKSFESLHRRVLSTLNGIENSKARLLSKAQCIMSGYKKVMWDITVNGITVHVPWRTPSQQN SLPLCEKGVETDAPSCSIQVDIKPKHEVNLSVKVMLNPLVVTYDSKFFLCLSEFFDELKSFESL HRRVLSTLNGIENSKARLLSKAQCIMSGYKKVMWDITVNGITVHVPWRTPSQQNSL MALQSTSLGVHLHNKIACLFVRKVLRPMLLPAVSKLTLNPNMRLTYQ*
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to salt stress in root

			
						 Other Information
					
						References
						Zhu et al., 2019;He et al., 2020

circRNA

Detail information of Csa1G652810_circ_g.2

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