Plant Circular RNA Database

Detail information of Os06g0149450_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os06g0149450_circ_g.3
					
						ID in PlantcircBase
						osa_circ_029684
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr6: 2560267-2561643  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os06g0149400						
					
						Parent gene annotation
						
							Similar to Chitinase A. (Os06t0149400-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os06g0149450_circ_g.1 Os06g0149450_circ_g.2 Os06g0149450_circ_g.4 Os06g0149450_circ_g.5 						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os06t0149450-00:6
Os06t0149450-00:6
Os06t0149400-01:7

General Information
CircRNA Name	Os06g0149450_circ_g.3
ID in PlantcircBase	osa_circ_029684
Alias	NA
Organism	Oryza sativa
Position	chr6: 2560267-2561643 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os06g0149400
Parent gene annotation	Similar to Chitinase A. (Os06t0149400-01)
Parent gene strand	+
Alternative splicing	Os06g0149450_circ_g.1 Os06g0149450_circ_g.2 Os06g0149450_circ_g.4 Os06g0149450_circ_g.5
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os06t0149450-00:6 Os06t0149450-00:6 Os06t0149400-01:7

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AGAAGATGATGAAGACGATGATGAAGATGAGGAGGAGGAAGAAGAGGATGAGGATGAAGACGAA
GACGATGAGGAAGAAAAAAGCAAGCCAAAGAAGAAGggccagcatgatgaaatcgagttaaaat
tctttgaagagagagctgcgcttgaagcgaagtaccagaagctttatgaacccttgtacacaaa
ggtatact

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GGCCAGCATGATGAAATCGAGTTAAAATTCTTTGAAGAGAGAGCTGCGCTTGAAGCGAAGTACC
AGAAGCTTTATGAACCCTTGTACACAAAGGTATACTTGTTCTTTATGTTAAATATGTGAACAGT
ATTCCTTTTGTTATTTTCTTTTGTGACAGTTACTGAACCCCTGCCCCTTTTCCTGTTTTCCAGA
GGTATAACATAGTAAATGGAGTTGTTGAGGTTGATGGTGGAAATGACGAACCAGCCAGTGAAAA
TGCTGCTGAGGGTAAAGATGCAGATGGTACCAAATCAAGTTTAATTTTATCTGTTGGTGAATGT
TTCACATTCTGATTTATGATTAAACCATTTTTTTTCAGCTAAAGGAGTCCCAGATTTTTGGCTT
ACTGCTATGAAAACAAATGAAGTGCTTTCTGAGGAGGTATAGTATGGTTTACTTTTGTGTTGTT
TTTTAGGTGCATTGCAGTAATTGTTGATTGTGTTATTGCTTTATTAGATCCAAGAGCGTGACGA
GCCTGCTTTGAAATATCTGAAAGATATCAAGTGGGCTAGAATTGACGATCCTAAGGGTTTCAAG
CTGGATTTTTTCTTTGATACGAACCCTTTCTTCAAGAACTCTGTCCTAACAAAAACGTACCACA
TGGTTGATGAAGATGAACCAATTTTGGAGAAAGCAATTGGGTAAGTTGTTTCATCCTCTGTTGA
AACCTGTAACTGCACGTGTTGTTAAAACTAACCATGTTGGCATGTTATGTGCACTGCAGGACTG
AAATTGAGTGGTATCCTGGTAAAAACCTGACACAGAAGATTCTAAAAAAGAAACCCAAGAAAGG
TTCAAAGAATGCAAAGCCTATTACCAAAACTGAAGTCTGTGAGAGCTTCTTCAACTTTTTCAGT
CCCCCGCAAGTACCCGATGATGATGAGGACATTGATGAAGACACTGTATGTTCTATTTTGATAT
TTGACATGCATTTGTTCAATGATATCATATTTGGTGTGCTTTGGTAGTAAACTTGTTCCTTCAA
TTTATGATAGGCTGATGAGCTTCAGGGTCAAATGGAACATGATTATGACATTGGGTAAGTACTA
GTCATTGCCTGCCACATACTAATGTTCTATCCGTCATGTTGATAAGCTCTTATGGCTATTCTGA
TGATATTTTTGGTCGGAAATTACTTATAGGACCACCATAAGGGATAAGATCATCCCACATGCTG
TTTCCTGGTTTACTGGTGAGGCTGTGCAAGCCGAGGATTTTGACGATATGGAGGATGATGAAGA
AGATGATGAAGACGATGATGAAGATGAGGAGGAGGAAGAAGAGGATGAGGATGAAGACGAAGAC
GATGAGGAAGAAAAAAGCAAGCCAAAGAAGAAG

Sequences
Splice junction sequence	AGAAGATGATGAAGACGATGATGAAGATGAGGAGGAGGAAGAAGAGGATGAGGATGAAGACGAA GACGATGAGGAAGAAAAAAGCAAGCCAAAGAAGAAGggccagcatgatgaaatcgagttaaaat tctttgaagagagagctgcgcttgaagcgaagtaccagaagctttatgaacccttgtacacaaa ggtatact
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GGCCAGCATGATGAAATCGAGTTAAAATTCTTTGAAGAGAGAGCTGCGCTTGAAGCGAAGTACC AGAAGCTTTATGAACCCTTGTACACAAAGGTATACTTGTTCTTTATGTTAAATATGTGAACAGT ATTCCTTTTGTTATTTTCTTTTGTGACAGTTACTGAACCCCTGCCCCTTTTCCTGTTTTCCAGA GGTATAACATAGTAAATGGAGTTGTTGAGGTTGATGGTGGAAATGACGAACCAGCCAGTGAAAA TGCTGCTGAGGGTAAAGATGCAGATGGTACCAAATCAAGTTTAATTTTATCTGTTGGTGAATGT TTCACATTCTGATTTATGATTAAACCATTTTTTTTCAGCTAAAGGAGTCCCAGATTTTTGGCTT ACTGCTATGAAAACAAATGAAGTGCTTTCTGAGGAGGTATAGTATGGTTTACTTTTGTGTTGTT TTTTAGGTGCATTGCAGTAATTGTTGATTGTGTTATTGCTTTATTAGATCCAAGAGCGTGACGA GCCTGCTTTGAAATATCTGAAAGATATCAAGTGGGCTAGAATTGACGATCCTAAGGGTTTCAAG CTGGATTTTTTCTTTGATACGAACCCTTTCTTCAAGAACTCTGTCCTAACAAAAACGTACCACA TGGTTGATGAAGATGAACCAATTTTGGAGAAAGCAATTGGGTAAGTTGTTTCATCCTCTGTTGA AACCTGTAACTGCACGTGTTGTTAAAACTAACCATGTTGGCATGTTATGTGCACTGCAGGACTG AAATTGAGTGGTATCCTGGTAAAAACCTGACACAGAAGATTCTAAAAAAGAAACCCAAGAAAGG TTCAAAGAATGCAAAGCCTATTACCAAAACTGAAGTCTGTGAGAGCTTCTTCAACTTTTTCAGT CCCCCGCAAGTACCCGATGATGATGAGGACATTGATGAAGACACTGTATGTTCTATTTTGATAT TTGACATGCATTTGTTCAATGATATCATATTTGGTGTGCTTTGGTAGTAAACTTGTTCCTTCAA TTTATGATAGGCTGATGAGCTTCAGGGTCAAATGGAACATGATTATGACATTGGGTAAGTACTA GTCATTGCCTGCCACATACTAATGTTCTATCCGTCATGTTGATAAGCTCTTATGGCTATTCTGA TGATATTTTTGGTCGGAAATTACTTATAGGACCACCATAAGGGATAAGATCATCCCACATGCTG TTTCCTGGTTTACTGGTGAGGCTGTGCAAGCCGAGGATTTTGACGATATGGAGGATGATGAAGA AGATGATGAAGACGATGATGAAGATGAGGAGGAGGAAGAAGAGGATGAGGATGAAGACGAAGAC GATGAGGAAGAAAAAAGCAAGCCAAAGAAGAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.287661529

Conservation Information
Conserved circRNAs	NA
PMCS	0.287661529

			
				
						 Functional Information
					

				
						Coding potential
						Y
					

						Potential coding position
						
							2560658-2561640(+)
2561478-2560288(+)
2560277-2561102(-)
						
					

						Potential amino acid sequence
						
							MKTNEVLSEEIQERDEPALKYLKDIKWARIDDPKGFKLDFFFDTNPFFKNSVLTKTYHMVDEDE
PILEKAIGTEIEWYPGKNLTQKILKKKPKKGSKNAKPITKTEVCESFFNFFSPPQVPDDDEDID
EDTADELQGQMEHDYDIGTTIRDKIIPHAVSWFTGEAVQAEDFDDMEDDEEDDEDDDEDEEEEE
EDEDEDEDDEEEKSKPKKKGQHDEIELKFFEERAALEAKYQKLYEPLYTKRYNIVNGVVEVDGG
NDEPASENAAEGKDADAKGVPDFWLTAMKTNEVLSEEIQERDEPALKYLKDIKWARIDDPKGFK
LDFFFDTNPFFKNSVLTKTYHMVDEDEPILEKAIGTEIEWYPGKNLTQKILKKKPKKGSKNAKP
ITKTEVCESFFNFFSPPQVPDDDEDIDEDTADELQGQMEHDYDIGTTIRDKIIPHAVSWFTGEA
VQAEDFDDMEDDEEDDEDDDEDEEEEEEDEDEDEDDEEEKSKPKKKGQHDEIELKFFEERAALE
AKYQKLYEPLYTKRYNIVNGVVEVDGGNDEPASENAAEGKDADAKGVPDFWLTAMKTNEVLSEE
IQERDEPALKYLKDIKWARIDDPKGFKLDFFFDTNPFFKNSVLTKTYHMVDEDEPILEKAIGTE
IEWYPGKNLTQKILKKKPKKGSKNAKPITKTEVCESFFNFFSPPQVPDDDEDIDEDTADELQGQ
MEHDYDIGTTIRDKIIPHAVSWFTGEAVQAEDFDDMEDDEEDDEDDDEDEEEEEEDEDEDEDDE
EEKSKPKKKGQHDEIELKFFEERAALEAKYQKLYEPLYTKRYNIVNGVVEVDGGNDEPASENAA
EGKDADAKGVPDFWLTAMKTNEVLSEEIQERDEPALKYLKDIKWARIDDPKGFKLDFFFDTNPF
FKNSVLTKTYHMVDEDEPILEKAIGTEIEWYPGKNLTQKILKKKPKKGSKNAKPITKTEVCESF
FNFFSPPQVPDDDEDIDEDTADELQGQMEHDYDIGTTIRDKIIPHAVSWFTGEAVQAEDFDDME
DDEEDDEDDDEDEEEEEEDEDEDEDDEEEKSKPKKK(+)
MLFPGLLVRLCKPRILTIWRMMKKMMKTMMKMRRRKKRMRMKTKTMRKKKASQRRRASMMKSS*
(+)
MLALLLWLAFFFLIVFVFILILFFLLLIFIIVFIIFFIILHIVKILGLHSLTSKPGNSMWDDLI
PYGGPNVIIMFHLTLKLISSVFINVLIIIGYLRGTEKVEEALTDFSFGNRLCIL*(-)
						
					

						Sponge-miRNAs
						
							osa-miR414 						
					

						circRNA-miRNA-mRNA network
						 VISUALIZATION
					

						Potential function description
						NA
					

			
		

Functional Information
Coding potential	Y
Potential coding position	2560658-2561640(+) 2561478-2560288(+) 2560277-2561102(-)
Potential amino acid sequence	MKTNEVLSEEIQERDEPALKYLKDIKWARIDDPKGFKLDFFFDTNPFFKNSVLTKTYHMVDEDE PILEKAIGTEIEWYPGKNLTQKILKKKPKKGSKNAKPITKTEVCESFFNFFSPPQVPDDDEDID EDTADELQGQMEHDYDIGTTIRDKIIPHAVSWFTGEAVQAEDFDDMEDDEEDDEDDDEDEEEEE EDEDEDEDDEEEKSKPKKKGQHDEIELKFFEERAALEAKYQKLYEPLYTKRYNIVNGVVEVDGG NDEPASENAAEGKDADAKGVPDFWLTAMKTNEVLSEEIQERDEPALKYLKDIKWARIDDPKGFK LDFFFDTNPFFKNSVLTKTYHMVDEDEPILEKAIGTEIEWYPGKNLTQKILKKKPKKGSKNAKP ITKTEVCESFFNFFSPPQVPDDDEDIDEDTADELQGQMEHDYDIGTTIRDKIIPHAVSWFTGEA VQAEDFDDMEDDEEDDEDDDEDEEEEEEDEDEDEDDEEEKSKPKKKGQHDEIELKFFEERAALE AKYQKLYEPLYTKRYNIVNGVVEVDGGNDEPASENAAEGKDADAKGVPDFWLTAMKTNEVLSEE IQERDEPALKYLKDIKWARIDDPKGFKLDFFFDTNPFFKNSVLTKTYHMVDEDEPILEKAIGTE IEWYPGKNLTQKILKKKPKKGSKNAKPITKTEVCESFFNFFSPPQVPDDDEDIDEDTADELQGQ MEHDYDIGTTIRDKIIPHAVSWFTGEAVQAEDFDDMEDDEEDDEDDDEDEEEEEEDEDEDEDDE EEKSKPKKKGQHDEIELKFFEERAALEAKYQKLYEPLYTKRYNIVNGVVEVDGGNDEPASENAA EGKDADAKGVPDFWLTAMKTNEVLSEEIQERDEPALKYLKDIKWARIDDPKGFKLDFFFDTNPF FKNSVLTKTYHMVDEDEPILEKAIGTEIEWYPGKNLTQKILKKKPKKGSKNAKPITKTEVCESF FNFFSPPQVPDDDEDIDEDTADELQGQMEHDYDIGTTIRDKIIPHAVSWFTGEAVQAEDFDDME DDEEDDEDDDEDEEEEEEDEDEDEDDEEEKSKPKKK(+) MLFPGLLVRLCKPRILTIWRMMKKMMKTMMKMRRRKKRMRMKTKTMRKKKASQRRRASMMKSS* (+) MLALLLWLAFFFLIVFVFILILFFLLLIFIIVFIIFFIILHIVKILGLHSLTSKPGNSMWDDLI PYGGPNVIIMFHLTLKLISSVFINVLIIIGYLRGTEKVEEALTDFSFGNRLCIL*(-)
Sponge-miRNAs	osa-miR414
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os06g0149450_circ_g.3

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