Plant Circular RNA Database

Detail information of Os01g0925000_circ_g.5

			
						 General Information
					
						CircRNA Name
						Os01g0925000_circ_g.5
					
						ID in PlantcircBase
						osa_circ_005598
					
						Alias
						Os_ciR1599
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 40552701-40553612  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder, circRNA_finder, find_circ
					
						Parent gene
						
							Os01g0925000						
					
						Parent gene annotation
						
							ENTH/VHS domain containing protein. (Os01t0925000-01);ENTH/VHS d
omain containing protein. (Os01t0925000-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os01g0925000_circ_g.2 Os01g0925000_circ_g.3 Os01g0925000_circ_g.4 						
					
						Support reads
						8/4
					
						Tissues
						root/shoot, root, seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0925000-01:2
Os01t0925000-02:2

General Information
CircRNA Name	Os01g0925000_circ_g.5
ID in PlantcircBase	osa_circ_005598
Alias	Os_ciR1599
Organism	Oryza sativa
Position	chr1: 40552701-40553612 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer, PcircRNA_finder, circRNA_finder, find_circ
Parent gene	Os01g0925000
Parent gene annotation	ENTH/VHS domain containing protein. (Os01t0925000-01);ENTH/VHS d omain containing protein. (Os01t0925000-02)
Parent gene strand	-
Alternative splicing	Os01g0925000_circ_g.2 Os01g0925000_circ_g.3 Os01g0925000_circ_g.4
Support reads	8/4
Tissues	root/shoot, root, seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0925000-01:2 Os01t0925000-02:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CAAAAGCTTCTGCGCCAAGATCTGTGGGCTGAATCCTGCATTCATGTTATGTAATCTAGCTTGG
GCGTTGAGAATCCCCCAAATTCCAGAGGTAAGAAACcagtctctgtacgacattccttccaaaa
tcatcgccgttgacaaacacatcgtttaaggcagcagggataaccctccagaactcatttacat
atctacca

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 311
Transcript exons: 40552701-40552822,40553418-40553518,40553525-40553612
CAGTCTCTGTACGACATTCCTTCCAAAATCATCGCCGTTGACAAACACATCGTTTAAGGCAGCA
GGGATAACCCTCCAGAACTCATTTACATATCTACCAGAGTCCTTTTTGCTGTTCTGCAATTAGA
AAGGACGTCACTAAGTTAATGATGGAGAAAGTTGTAGGATCACACTCTGGAAGGATACTCTCTA
TACTTTGTCGTGAATTATTGAGCTTCAAAAGCGCCAAGATCTGTGGGCTGAATCCTGCATTCAT
GTTATGTAATCTAGCTTGGGCGTTGAGAATCCCCCAAATTCCAGAGGTAAGAAAC
Transcript length: 379
Transcript exons: 40552701-40552948,40553482-40553612
CAGTCTCTGTACGACATTCCTTCCAAAATCATCGCCGTTGACAAACACATCGTTTAAGGCAGCA
GGGATAACCCTCCAGAACTCATTTACATATCTACCAGAGTCCTTTTTGCTGTTCTGCACGATAT
CATTTGCAAGGTACAGCAAAGATACCCTCCTCTCACGAGGAGCTGAGTGAAAATCAGATTCCCA
TGTTTCAACAACTTGTCGGCAGTGTCGATAATGAAAGACACACCAATGTGACAAAGTCTCTATA
CTTTGTCGTGAATTATTGAGCTTCAAAAGCTTCTGCGCCAAGATCTGTGGGCTGAATCCTGCAT
TCATGTTATGTAATCTAGCTTGGGCGTTGAGAATCCCCCAAATTCCAGAGGTAAGAAAC
						
					

						Genomic sequence
						
							CAGTCTCTGTACGACATTCCTTCCAAAATCATCGCCGTTGACAAACACATCGTTTAAGGCAGCA
GGGATAACCCTCCAGAACTCATTTACATATCTACCAGAGTCCTTTTTGCTGTTCTGCACGATAT
CATTTGCAAGGTACAGCAAAGATACCCTCCTCTCACGAGGAGCTGAGTGAAAATCAGATTCCCA
TGTTTCAACAACTTGTCGGCAGTGTCGATAATGAAAGACACACCAATGTGACAAAGCTTTTCTT
TTGTTAAGCATCACCATTATGTGAATGAGCAGTATAATGAGTATAATGTACTAGCCATGAGGTA
AGATCAAATAAACAATCAGAAATAAGCTGTCAAATTTTGTTTGGACACACTTCTTAGGAAGAAC
ATATTTGAGCAAGGGTCAACGGATGACTGCTAGACTACCAGAATGATATCAACTGATCATAATA
CTGTGTGATGAGTGATCAACTGGATGCAAAAGGTCTACTGCCATAGTTATAAAAGTGGTAAGAT
TAAAATTTTAAAGCATTAACCTTTTCTATAAAATGTGTGCTATGAATTTTAATCATCCAGGCCA
TGTAAGCCACTGAATTGTAACTGACAAGTCAAAATGAAGATGGATAGTTTGTGTGTTATCCTGA
GAGAAACTATAGGGAAAATAGTTGACACCCTCTTATCCATGAAGAACAATAGCTATGCGACAAT
TACGCTAAACCACATTAGAAAGGACGTCACTAAGTTAATGATGGAGAAAGTTGTAGGATCACAC
TCTGGAAGGATACTCTCTATACTTTGTCGTGAATTATTGAGCTTCAAAAGCTTCTGCGCCAAGA
TCTGTGGGCTGAATCCTGCATTCATGTTATGTAATCTAGCTTGGGCGTTGAGAATCCCCCAAAT
TCCAGAGGTAAGAAAC
						
					

			
		

Sequences
Splice junction sequence	CAAAAGCTTCTGCGCCAAGATCTGTGGGCTGAATCCTGCATTCATGTTATGTAATCTAGCTTGG GCGTTGAGAATCCCCCAAATTCCAGAGGTAAGAAACcagtctctgtacgacattccttccaaaa tcatcgccgttgacaaacacatcgtttaaggcagcagggataaccctccagaactcatttacat atctacca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 311 Transcript exons: 40552701-40552822,40553418-40553518,40553525-40553612 CAGTCTCTGTACGACATTCCTTCCAAAATCATCGCCGTTGACAAACACATCGTTTAAGGCAGCA GGGATAACCCTCCAGAACTCATTTACATATCTACCAGAGTCCTTTTTGCTGTTCTGCAATTAGA AAGGACGTCACTAAGTTAATGATGGAGAAAGTTGTAGGATCACACTCTGGAAGGATACTCTCTA TACTTTGTCGTGAATTATTGAGCTTCAAAAGCGCCAAGATCTGTGGGCTGAATCCTGCATTCAT GTTATGTAATCTAGCTTGGGCGTTGAGAATCCCCCAAATTCCAGAGGTAAGAAAC Transcript length: 379 Transcript exons: 40552701-40552948,40553482-40553612 CAGTCTCTGTACGACATTCCTTCCAAAATCATCGCCGTTGACAAACACATCGTTTAAGGCAGCA GGGATAACCCTCCAGAACTCATTTACATATCTACCAGAGTCCTTTTTGCTGTTCTGCACGATAT CATTTGCAAGGTACAGCAAAGATACCCTCCTCTCACGAGGAGCTGAGTGAAAATCAGATTCCCA TGTTTCAACAACTTGTCGGCAGTGTCGATAATGAAAGACACACCAATGTGACAAAGTCTCTATA CTTTGTCGTGAATTATTGAGCTTCAAAAGCTTCTGCGCCAAGATCTGTGGGCTGAATCCTGCAT TCATGTTATGTAATCTAGCTTGGGCGTTGAGAATCCCCCAAATTCCAGAGGTAAGAAAC
Genomic sequence	CAGTCTCTGTACGACATTCCTTCCAAAATCATCGCCGTTGACAAACACATCGTTTAAGGCAGCA GGGATAACCCTCCAGAACTCATTTACATATCTACCAGAGTCCTTTTTGCTGTTCTGCACGATAT CATTTGCAAGGTACAGCAAAGATACCCTCCTCTCACGAGGAGCTGAGTGAAAATCAGATTCCCA TGTTTCAACAACTTGTCGGCAGTGTCGATAATGAAAGACACACCAATGTGACAAAGCTTTTCTT TTGTTAAGCATCACCATTATGTGAATGAGCAGTATAATGAGTATAATGTACTAGCCATGAGGTA AGATCAAATAAACAATCAGAAATAAGCTGTCAAATTTTGTTTGGACACACTTCTTAGGAAGAAC ATATTTGAGCAAGGGTCAACGGATGACTGCTAGACTACCAGAATGATATCAACTGATCATAATA CTGTGTGATGAGTGATCAACTGGATGCAAAAGGTCTACTGCCATAGTTATAAAAGTGGTAAGAT TAAAATTTTAAAGCATTAACCTTTTCTATAAAATGTGTGCTATGAATTTTAATCATCCAGGCCA TGTAAGCCACTGAATTGTAACTGACAAGTCAAAATGAAGATGGATAGTTTGTGTGTTATCCTGA GAGAAACTATAGGGAAAATAGTTGACACCCTCTTATCCATGAAGAACAATAGCTATGCGACAAT TACGCTAAACCACATTAGAAAGGACGTCACTAAGTTAATGATGGAGAAAGTTGTAGGATCACAC TCTGGAAGGATACTCTCTATACTTTGTCGTGAATTATTGAGCTTCAAAAGCTTCTGCGCCAAGA TCTGTGGGCTGAATCCTGCATTCATGTTATGTAATCTAGCTTGGGCGTTGAGAATCCCCCAAAT TCCAGAGGTAAGAAAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_002648* osi_circ_011137 						
					
						PMCS
						0.501550014

Conservation Information
Conserved circRNAs	osi_circ_002648* osi_circ_011137
PMCS	0.501550014

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							40553557-40552754(+)
40553559-40553570(-)
40552752-40553570(-)
						
						Potential amino acid sequence
						
							MLCNLAWALRIPQIPEVRNQSLYDIPSKIIAVDKHIV*(+)
MNAGFSPQILAQKLLKLNNSRQSIETLSHWCVFHYRHCRQVVETWESDFHSAPRERRVSLLYLA
NDIVQNSKKDSGRYVNEFWRVIPAALNDVFVNGDDFGRNVVQRLVSYLWNLGDSQRPS*(-)
MCLSTAMILEGMSYRDWFLTSGIWGILNAQARLHNMNAGFSPQILAQKLLKLNNSRQSIETLSH
WCVFHYRHCRQVVETWESDFHSAPRERRVSLLYLANDIVQNSKKDSGRYVNEFWRVIPAALNDV
FVNGDDFGRNVVQRLVSYLWNLGDSQRPS*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	40553557-40552754(+) 40553559-40553570(-) 40552752-40553570(-)
Potential amino acid sequence	MLCNLAWALRIPQIPEVRNQSLYDIPSKIIAVDKHIV(+) MNAGFSPQILAQKLLKLNNSRQSIETLSHWCVFHYRHCRQVVETWESDFHSAPRERRVSLLYLA NDIVQNSKKDSGRYVNEFWRVIPAALNDVFVNGDDFGRNVVQRLVSYLWNLGDSQRPS(-) MCLSTAMILEGMSYRDWFLTSGIWGILNAQARLHNMNAGFSPQILAQKLLKLNNSRQSIETLSH WCVFHYRHCRQVVETWESDFHSAPRERRVSLLYLANDIVQNSKKDSGRYVNEFWRVIPAALNDV FVNGDDFGRNVVQRLVSYLWNLGDSQRPS*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os01g0925000_circ_g.5

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