Plant Circular RNA Database

Detail information of BGIOSGA000221_circ_g.2

			
						 General Information
					
						CircRNA Name
						BGIOSGA000221_circ_g.2
					
						ID in PlantcircBase
						osi_circ_002648
					
						Alias
						1:44340952|44341863
					
						Organism
						
							Oryza sativa ssp. indica						
					
						Position
						
							chr1: 44340952-44341863  JBrowse»						
					
						Reference genome
						
							Oryza_indica.ASM465v1.42						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							BGIOSGA000221						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						-
					
						Alternative splicing
						
							BGIOSGA000222_circ_g.2 BGIOSGA000222_circ_ag.1 BGIOSGA000222_circ_ag.2 BGIOSGA000221_circ_g.1 						
					
						Support reads
						NA
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						BGIOSGA000221-TA:2

General Information
CircRNA Name	BGIOSGA000221_circ_g.2
ID in PlantcircBase	osi_circ_002648
Alias	1:44340952\|44341863
Organism	Oryza sativa ssp. indica
Position	chr1: 44340952-44341863 JBrowse»
Reference genome	Oryza_indica.ASM465v1.42
Type	e-circRNA
Identification method	find_circ
Parent gene	BGIOSGA000221
Parent gene annotation	NA
Parent gene strand	-
Alternative splicing	BGIOSGA000222_circ_g.2 BGIOSGA000222_circ_ag.1 BGIOSGA000222_circ_ag.2 BGIOSGA000221_circ_g.1
Support reads	NA
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	BGIOSGA000221-TA:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CAAAAGCTTCTGCGCCAAGATCTGTGGGCTGAATCCTGCATTCATGTTATGTAATCTAGCTTGG
GCGTTGAGAATCCCCCAAATTCCAGAGGTAAGAAACcagtctctgtacgacattccttccaaaa
tcatcaccattgacaaacacatcgtttaaggcagcagggatgaccctccagaactcatttacat
atctacca

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							CAGTCTCTGTACGACATTCCTTCCAAAATCATCACCATTGACAAACACATCGTTTAAGGCAGCA
GGGATGACCCTCCAGAACTCATTTACATATCTACCAGAGTCCTTTTTGCTGTTCTGAACGATAT
CATTTGCAAGGTACAGCAAAGATACCCTCCTCTCACGAGGAGCTGAGTGAAAATCAGATTCCCA
TGTTTCAACAACTTGTCGGCAGTGTCGATAATGAAAGACACACCAATGTGACAAAGCTTTTCTT
TTGTTAAGCATCACCATCATGTGAATGAGCAGTATAATGAGTATAATGTACTAGCCATGAGGTA
AGATCAAATAAACAATCAGAAATAAACTGTCAAATTTTGTTTGGACACACTTCTTAGGAAGAAC
ATATTTGAGCAAGGGTCAACGGATGACTGCTAGACTACCAGAATGATATCAACTGATCATAATA
CTGTGTGATGAGTGATCAACTGGATGCAAAAGGTCTACTGCCATAGTTATAAAAGTGGTAAGAT
TAAAATTCGAAAGCATTAACCTTTTCTATAAAATGTGTGCTATGAATTTTAATCATCCAGGCCA
TGTAAGCCACTGAATTGTAACAGACAAGTCAAAATGAAGATGGATAGTTTGTGTGTTATCCTGA
GAGAAACTATAGGGAAAATAGTTGACACCCTCTTATCCATGAAGAACAATAGCTATGCGACAAT
TACGCTAAACCACATTAGAAAGGACGTCACTAAGTTAATGATGGAGAAAGTTGTAGGATCACAC
TCTGGAAGGATACTCTCTATACTTTGTCGTGAATTATTGAGCTTCAAAAGCTTCTGCGCCAAGA
TCTGTGGGCTGAATCCTGCATTCATGTTATGTAATCTAGCTTGGGCGTTGAGAATCCCCCAAAT
TCCAGAGGTAAGAAAC

Sequences
Splice junction sequence	CAAAAGCTTCTGCGCCAAGATCTGTGGGCTGAATCCTGCATTCATGTTATGTAATCTAGCTTGG GCGTTGAGAATCCCCCAAATTCCAGAGGTAAGAAACcagtctctgtacgacattccttccaaaa tcatcaccattgacaaacacatcgtttaaggcagcagggatgaccctccagaactcatttacat atctacca
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CAGTCTCTGTACGACATTCCTTCCAAAATCATCACCATTGACAAACACATCGTTTAAGGCAGCA GGGATGACCCTCCAGAACTCATTTACATATCTACCAGAGTCCTTTTTGCTGTTCTGAACGATAT CATTTGCAAGGTACAGCAAAGATACCCTCCTCTCACGAGGAGCTGAGTGAAAATCAGATTCCCA TGTTTCAACAACTTGTCGGCAGTGTCGATAATGAAAGACACACCAATGTGACAAAGCTTTTCTT TTGTTAAGCATCACCATCATGTGAATGAGCAGTATAATGAGTATAATGTACTAGCCATGAGGTA AGATCAAATAAACAATCAGAAATAAACTGTCAAATTTTGTTTGGACACACTTCTTAGGAAGAAC ATATTTGAGCAAGGGTCAACGGATGACTGCTAGACTACCAGAATGATATCAACTGATCATAATA CTGTGTGATGAGTGATCAACTGGATGCAAAAGGTCTACTGCCATAGTTATAAAAGTGGTAAGAT TAAAATTCGAAAGCATTAACCTTTTCTATAAAATGTGTGCTATGAATTTTAATCATCCAGGCCA TGTAAGCCACTGAATTGTAACAGACAAGTCAAAATGAAGATGGATAGTTTGTGTGTTATCCTGA GAGAAACTATAGGGAAAATAGTTGACACCCTCTTATCCATGAAGAACAATAGCTATGCGACAAT TACGCTAAACCACATTAGAAAGGACGTCACTAAGTTAATGATGGAGAAAGTTGTAGGATCACAC TCTGGAAGGATACTCTCTATACTTTGTCGTGAATTATTGAGCTTCAAAAGCTTCTGCGCCAAGA TCTGTGGGCTGAATCCTGCATTCATGTTATGTAATCTAGCTTGGGCGTTGAGAATCCCCCAAAT TCCAGAGGTAAGAAAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osa_circ_005598* osa_circ_005597 						
					
						PMCS

Conservation Information
Conserved circRNAs	osa_circ_005598* osa_circ_005597
PMCS

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							44341810-44341821(-)
44341003-44341821(-)
44341808-44341005(+)
						
						Potential amino acid sequence
						
							MNAGFSPQILAQKLLKLNNSRQSIETLSHWCVFHYRHCRQVVETWESDFHSAPRERRVSLLYLA
NDIVQNSKKDSGRYVNEFWRVIPAALNDVFVNGDDFGRNVVQRLVSYLWNLGDSQRPS*(-)
MCLSMVMILEGMSYRDWFLTSGIWGILNAQARLHNMNAGFSPQILAQKLLKLNNSRQSIETLSH
WCVFHYRHCRQVVETWESDFHSAPRERRVSLLYLANDIVQNSKKDSGRYVNEFWRVIPAALNDV
FVNGDDFGRNVVQRLVSYLWNLGDSQRPS*(-)
MLCNLAWALRIPQIPEVRNQSLYDIPSKIITIDKHIV*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	44341810-44341821(-) 44341003-44341821(-) 44341808-44341005(+)
Potential amino acid sequence	MNAGFSPQILAQKLLKLNNSRQSIETLSHWCVFHYRHCRQVVETWESDFHSAPRERRVSLLYLA NDIVQNSKKDSGRYVNEFWRVIPAALNDVFVNGDDFGRNVVQRLVSYLWNLGDSQRPS(-) MCLSMVMILEGMSYRDWFLTSGIWGILNAQARLHNMNAGFSPQILAQKLLKLNNSRQSIETLSH WCVFHYRHCRQVVETWESDFHSAPRERRVSLLYLANDIVQNSKKDSGRYVNEFWRVIPAALNDV FVNGDDFGRNVVQRLVSYLWNLGDSQRPS(-) MLCNLAWALRIPQIPEVRNQSLYDIPSKIITIDKHIV*(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Huang et al., 2021

circRNA

Detail information of BGIOSGA000221_circ_g.2

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