Plant Circular RNA Database

Detail information of Os04g0102700_circ_g.7

			
						 General Information
					
						CircRNA Name
						Os04g0102700_circ_g.7
					
						ID in PlantcircBase
						osa_circ_022867
					
						Alias
						Os_ciR1874
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr4: 193516-194383  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os04g0102700						
					
						Parent gene annotation
						
							Similar to N-acylethanolamine amidohydrolase. (Os04t0102700-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os04g0102700_circ_g.6 Os04g0102700_circ_g.8 						
					
						Support reads
						6/1
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os04t0102700-01:4

General Information
CircRNA Name	Os04g0102700_circ_g.7
ID in PlantcircBase	osa_circ_022867
Alias	Os_ciR1874
Organism	Oryza sativa
Position	chr4: 193516-194383 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os04g0102700
Parent gene annotation	Similar to N-acylethanolamine amidohydrolase. (Os04t0102700-01)
Parent gene strand	-
Alternative splicing	Os04g0102700_circ_g.6 Os04g0102700_circ_g.8
Support reads	6/1
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os04t0102700-01:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CGCAGAGACGGATCATAGGGCGGGAGGCAGTGAAGTGCTTCCTCAACTCTGTCCACAGGGTCCC
TGTCATCCCCCAGAAGCAAAACTCCTTGCTCCGGCTccatagtttggattgtttccagttactc
caaggcctagctcatgcatattagctttcccaatgaataacactccacatttccgcaaacgagc
aacacaaa

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 547
Transcript exons: 193516-193663,193755-193832,193971-194100,194193-194383
CCATAGTTTGGATTGTTTCCAGTTACTCCAAGGCCTAGCTCATGCATATTAGCTTTCCCAATGA
ATAACACTCCACATTTCCGCAAACGAGCAACACAAACTGCGTCTTTCTCCACAGAGCGAATTTT
GTCGAAAAATGTAGTAGCACCCTTTGATGGATATGGGAAGCAGTCAATGTCATCCTTAATGGCG
ATAAAGATCCCGTCCAAGATGGAAATTGGGTTTCCTTGCTGAAATCTCTTTGTGGAAGCTTCAG
CTTGTTTCCTTAGATCATCTGCGTTAAAATAAACCAACATAGGCATGGGAGGCTTCTTGTTGCT
CCACTCTTCCACACCGGCAATGATATGCTCCGCAACAACAGACGGGGTTGTGATCCCGGAGCGG
TACGCATGCGCAAAATCACGGATCTTCCAGTAGAGGAAAGGGGGTTTGTCCCCGGCCGGCCAAC
GCAGAGACGGATCATAGGGCGGGAGGCAGTGAAGTGCTTCCTCAACTCTGTCCACAGGGTCCCT
GTCATCCCCCAGAAGCAAAACTCCTTGCTCCGGCT
						
						Genomic sequence
						
							CCATAGTTTGGATTGTTTCCAGTTACTCCAAGGCCTAGCTCATGCATATTAGCTTTCCCAATGA
ATAACACTCCACATTTCCGCAAACGAGCAACACAAACTGCGTCTTTCTCCACAGAGCGAATTTT
GTCGAAAAATGTAGTAGCACCTGTATTTTTTTTAAAAAAAAGGTATAAGTTGGAATACGCTTCT
CAGATACAGCAACCAAATTGCCAGGCCCCTTCAGTCTGTTAACTAACCCTTTGATGGATATGGG
AAGCAGTCAATGTCATCCTTAATGGCGATAAAGATCCCGTCCAAGATGGAAATTGGGTTTCCTG
AGCCAAGGAGCACCATCACATGTCAAGAAGATGAGATAGAATATGGTGGAGTTTCAATTTGAAA
GTATTTAACCCAAATATATATATAAAAAAGTCAACAATACAAAAATATCATGATTTTAGAGAAG
GGAAAACCTTGCTGAAATCTCTTTGTGGAAGCTTCAGCTTGTTTCCTTAGATCATCTGCGTTAA
AATAAACCAACATAGGCATGGGAGGCTTCTTGTTGCTCCACTCTTCCACACCGGCAATGATATG
CTCCGCAACCTATGCAACAAGAAGAAAGTCAAGTGGCACACATGCAGGAGGGGAGGCAGAGAAG
AAAATAATGATTTGTTCAATCCAATGGGGCTACTGACAACAGACGGGGTTGTGATCCCGGAGCG
GTACGCATGCGCAAAATCACGGATCTTCCAGTAGAGGAAAGGGGGTTTGTCCCCGGCCGGCCAA
CGCAGAGACGGATCATAGGGCGGGAGGCAGTGAAGTGCTTCCTCAACTCTGTCCACAGGGTCCC
TGTCATCCCCCAGAAGCAAAACTCCTTGCTCCGGCT

Sequences
Splice junction sequence	CGCAGAGACGGATCATAGGGCGGGAGGCAGTGAAGTGCTTCCTCAACTCTGTCCACAGGGTCCC TGTCATCCCCCAGAAGCAAAACTCCTTGCTCCGGCTccatagtttggattgtttccagttactc caaggcctagctcatgcatattagctttcccaatgaataacactccacatttccgcaaacgagc aacacaaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 547 Transcript exons: 193516-193663,193755-193832,193971-194100,194193-194383 CCATAGTTTGGATTGTTTCCAGTTACTCCAAGGCCTAGCTCATGCATATTAGCTTTCCCAATGA ATAACACTCCACATTTCCGCAAACGAGCAACACAAACTGCGTCTTTCTCCACAGAGCGAATTTT GTCGAAAAATGTAGTAGCACCCTTTGATGGATATGGGAAGCAGTCAATGTCATCCTTAATGGCG ATAAAGATCCCGTCCAAGATGGAAATTGGGTTTCCTTGCTGAAATCTCTTTGTGGAAGCTTCAG CTTGTTTCCTTAGATCATCTGCGTTAAAATAAACCAACATAGGCATGGGAGGCTTCTTGTTGCT CCACTCTTCCACACCGGCAATGATATGCTCCGCAACAACAGACGGGGTTGTGATCCCGGAGCGG TACGCATGCGCAAAATCACGGATCTTCCAGTAGAGGAAAGGGGGTTTGTCCCCGGCCGGCCAAC GCAGAGACGGATCATAGGGCGGGAGGCAGTGAAGTGCTTCCTCAACTCTGTCCACAGGGTCCCT GTCATCCCCCAGAAGCAAAACTCCTTGCTCCGGCT
Genomic sequence	CCATAGTTTGGATTGTTTCCAGTTACTCCAAGGCCTAGCTCATGCATATTAGCTTTCCCAATGA ATAACACTCCACATTTCCGCAAACGAGCAACACAAACTGCGTCTTTCTCCACAGAGCGAATTTT GTCGAAAAATGTAGTAGCACCTGTATTTTTTTTAAAAAAAAGGTATAAGTTGGAATACGCTTCT CAGATACAGCAACCAAATTGCCAGGCCCCTTCAGTCTGTTAACTAACCCTTTGATGGATATGGG AAGCAGTCAATGTCATCCTTAATGGCGATAAAGATCCCGTCCAAGATGGAAATTGGGTTTCCTG AGCCAAGGAGCACCATCACATGTCAAGAAGATGAGATAGAATATGGTGGAGTTTCAATTTGAAA GTATTTAACCCAAATATATATATAAAAAAGTCAACAATACAAAAATATCATGATTTTAGAGAAG GGAAAACCTTGCTGAAATCTCTTTGTGGAAGCTTCAGCTTGTTTCCTTAGATCATCTGCGTTAA AATAAACCAACATAGGCATGGGAGGCTTCTTGTTGCTCCACTCTTCCACACCGGCAATGATATG CTCCGCAACCTATGCAACAAGAAGAAAGTCAAGTGGCACACATGCAGGAGGGGAGGCAGAGAAG AAAATAATGATTTGTTCAATCCAATGGGGCTACTGACAACAGACGGGGTTGTGATCCCGGAGCG GTACGCATGCGCAAAATCACGGATCTTCCAGTAGAGGAAAGGGGGTTTGTCCCCGGCCGGCCAA CGCAGAGACGGATCATAGGGCGGGAGGCAGTGAAGTGCTTCCTCAACTCTGTCCACAGGGTCCC TGTCATCCCCCAGAAGCAAAACTCCTTGCTCCGGCT

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_014353 						
					
						PMCS
						0.277012126

Conservation Information
Conserved circRNAs	osi_circ_014353
PMCS	0.277012126

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							194090-193564(+)
193521-194352(-)
194048-194352(-)
						
						Potential amino acid sequence
						
							MLRNNRRGCDPGAVRMRKITDLPVEERGFVPGRPTQRRIIGREAVKCFLNSVHRVPVIPQKQNS
LLRLHSLDCFQLLQGLAHAY*(+)
MEPEQGVLLLGDDRDPVDRVEEALHCLPPYDPSLRWPAGDKPPFLYWKIRDFAHAYRSGITTPS
VVAEHIIAGVEEWSNKKPPMPMLVYFNADDLRKQAEASTKRFQQGNPISILDGIFIAIKDDIDC
FPYPSKGATTFFDKIRSVEKDAVCVARLRKCGVLFIGKANMHELGLGVTGNNPNYGAGARSFAS
GG*(-)
MPMLVYFNADDLRKQAEASTKRFQQGNPISILDGIFIAIKDDIDCFPYPSKGATTFFDKIRSVE
KDAVCVARLRKCGVLFIGKANMHELGLGVTGNNPNYGAGARSFASGG*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	194090-193564(+) 193521-194352(-) 194048-194352(-)
Potential amino acid sequence	MLRNNRRGCDPGAVRMRKITDLPVEERGFVPGRPTQRRIIGREAVKCFLNSVHRVPVIPQKQNS LLRLHSLDCFQLLQGLAHAY(+) MEPEQGVLLLGDDRDPVDRVEEALHCLPPYDPSLRWPAGDKPPFLYWKIRDFAHAYRSGITTPS VVAEHIIAGVEEWSNKKPPMPMLVYFNADDLRKQAEASTKRFQQGNPISILDGIFIAIKDDIDC FPYPSKGATTFFDKIRSVEKDAVCVARLRKCGVLFIGKANMHELGLGVTGNNPNYGAGARSFAS GG(-) MPMLVYFNADDLRKQAEASTKRFQQGNPISILDGIFIAIKDDIDCFPYPSKGATTFFDKIRSVE KDAVCVARLRKCGVLFIGKANMHELGLGVTGNNPNYGAGARSFASGG*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os04g0102700_circ_g.7

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