Plant Circular RNA Database

Detail information of GLYMA_11G035800_circ_g.2

			
						 General Information
					
						CircRNA Name
						GLYMA_11G035800_circ_g.2
					
						ID in PlantcircBase
						gma_circ_002912
					
						Alias
						Gm11circRNA2096, gma-circRNA1526
					
						Organism
						
							Glycine max						
					
						Position
						
							chr11: 2577854-2579263  JBrowse»						
					
						Reference genome
						
							v2.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						Tophat, CIRI, find_circ
					
						Parent gene
						
							GLYMA_11G035800						
					
						Parent gene annotation
						
							hypothetical protein
							
						Parent gene strand
						-
					
						Alternative splicing
						
							GLYMA_11G035800_circ_g.1 						
					
						Support reads
						NA
					
						Tissues
						root, stem, flower bud
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						KRH28154:3

General Information
CircRNA Name	GLYMA_11G035800_circ_g.2
ID in PlantcircBase	gma_circ_002912
Alias	Gm11circRNA2096, gma-circRNA1526
Organism	Glycine max
Position	chr11: 2577854-2579263 JBrowse»
Reference genome	v2.0.38
Type	e-circRNA
Identification method	Tophat, CIRI, find_circ
Parent gene	GLYMA_11G035800
Parent gene annotation	hypothetical protein
Parent gene strand	-
Alternative splicing	GLYMA_11G035800_circ_g.1
Support reads	NA
Tissues	root, stem, flower bud
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	KRH28154:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GTCCTCTTGCACCAATCCTTCACTACCACTTCATCCAACAAAAATGCTATGACAGGATCACTGG
AAACAGCAGTTTCATCCATACATACATCAGTGATGTaatggcctgttttgcaaccaccatatca
gagcctccatgtaaaaagagcaagtcaacagcagaacgaagatttttgaagggatagtaaccca
gcatctga

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 713
Transcript exons: 2577854-2578255,2578854-2579029,2579129-2579263
AATGGCCTGTTTTGCAACCACCATATCAGAGCCTCCATGTAAAAAGAGCAAGTCAACAGCAGAA
CGAAGATTTTTGAAGGGATAGTAACCCAGCATCTGATCAGTATTTGACCCCAAAAATGATGCAC
TATCCTTCTGCAAGGATGCAATTTCTAATCCCTCTACAATTTCATTCCTAAACCCCTCCTCCAG
ATCAAGATTATTCAATGCATCTTCAATAAATAGAGACCCATCATGTCCTTGTGACTCCACAGAT
TGATTTATTGCATCAGGCCATGCTCGTCTAATTGCTTCTGATTTTCGTATCCGAACAACTGATG
ACCATGATGAACCATCAGTAAAACGTGACCTCACACCTTCCAAAAATTGATGTCTTGTACACCA
AATAATAACATCCATATGCTGTTTTGTCTTCATGATGCTCTCTTGGAGATTCTGCAAATCGTGC
AGTTGTGCTGAAAGAGTACCCTTAAAAGATGACTCCAAAATGTCTAGCACATTCGATATGCCCT
TCAAGTATAATGAAAATTTCAGAAGTAAACTCAATCTCTCTTTCAAGCCTAAAATGTCCATATC
ATAAATTCCCTGCAGTTCAGCAATGATGTTTTTGAATGTCCTCTTGCACCAATCCTTCACTACC
ACTTCATCCAACAAAAATGCTATGACAGGATCACTGGAAACAGCAGTTTCATCCATACATACAT
CAGTGATGT
						
					

						Genomic sequence
						
							AATGGCCTGTTTTGCAACCACCATATCAGAGCCTCCATGTAAAAAGAGCAAGTCAACAGCAGAA
CGAAGATTTTTGAAGGGATAGTAACCCAGCATCTGATCAGTATTTGACCCCAAAAATGATGCAC
TATCCTTCTGCAAGGATGCAATTTCTAATCCCTCTACAATTTCATTCCTAAACCCCTCCTCCAG
ATCAAGATTATTCAATGCATCTTCAATAAATAGAGACCCATCATGTCCTTGTGACTCCACAGAT
TGATTTATTGCATCAGGCCATGCTCGTCTAATTGCTTCTGATTTTCGTATCCGAACAACTGATG
ACCATGATGAACCATCAGTAAAACGTGACCTCACACCTTCCAAAAATTGATGTCTTGTACACCA
AATAATAACATCCATATGCTGGGTAGAACAAAAACAAAAATTAAACACCAGGATGAAGATTGCA
ATGAGCTACTCTAATTTTTGGAGCTATATTAGAGTAAGTATTTAGTTACTAACAGATTGGGTTA
GTTAACAGTTAGTCTCGCTATATATAGAGATGACTGATAGTTAGTCTTTCTTTTTCTTGATCAA
TGAGAAATCAGAGTTTATCAAACTCTATTACAGACAGACAGATAGAGGAAGACTAAATCCAGCC
AATCACTCTTTATAAGCAATTAATTAAAGTGCAAGGCCAACTATCAGTCATTTCTACAAGCATC
CCTCTTACACACTTCATCATAGCGAACAACAACAAAGGTTACCCATACTCATCCACACTTTTGA
AGATTGGGTATAAAGCCCCTTCCAACAAAAATCACACCGAATCATTTGAAGAACACAATGCACA
AAAACAATAGCAATACCATTAAAAGAATAGATAATATGTACAAATTTATCATGATGACTTGACA
GCACATCAGCCAAGCAAGAAATGGGTCAAAAATCAAATATTAATGCAAAAATCATAACAGTTAT
GCAATAACTCCATTGAACATGAATTCTAGCAAAACCAAACCTGTTTTGTCTTCATGATGCTCTC
TTGGAGATTCTGCAAATCGTGCAGTTGTGCTGAAAGAGTACCCTTAAAAGATGACTCCAAAATG
TCTAGCACATTCGATATGCCCTTCAAGTATAATGAAAATTTCAGAAGTAAACTCAATCTCTCTT
TCAAGCCTAAAATGTCCATATCATCTAGTGAAAATTGTCATGGAGTGCAAACATAATTATGGAA
AAAACGTCATACTAATAATTAGCTTTAGATATCAACAATAAAACAAGGTTTAAGGATACAAATT
CCCTGCAGTTCAGCAATGATGTTTTTGAATGTCCTCTTGCACCAATCCTTCACTACCACTTCAT
CCAACAAAAATGCTATGACAGGATCACTGGAAACAGCAGTTTCATCCATACATACATCAGTGAT
GT
						
					

			
		

Sequences
Splice junction sequence	GTCCTCTTGCACCAATCCTTCACTACCACTTCATCCAACAAAAATGCTATGACAGGATCACTGG AAACAGCAGTTTCATCCATACATACATCAGTGATGTaatggcctgttttgcaaccaccatatca gagcctccatgtaaaaagagcaagtcaacagcagaacgaagatttttgaagggatagtaaccca gcatctga
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 713 Transcript exons: 2577854-2578255,2578854-2579029,2579129-2579263 AATGGCCTGTTTTGCAACCACCATATCAGAGCCTCCATGTAAAAAGAGCAAGTCAACAGCAGAA CGAAGATTTTTGAAGGGATAGTAACCCAGCATCTGATCAGTATTTGACCCCAAAAATGATGCAC TATCCTTCTGCAAGGATGCAATTTCTAATCCCTCTACAATTTCATTCCTAAACCCCTCCTCCAG ATCAAGATTATTCAATGCATCTTCAATAAATAGAGACCCATCATGTCCTTGTGACTCCACAGAT TGATTTATTGCATCAGGCCATGCTCGTCTAATTGCTTCTGATTTTCGTATCCGAACAACTGATG ACCATGATGAACCATCAGTAAAACGTGACCTCACACCTTCCAAAAATTGATGTCTTGTACACCA AATAATAACATCCATATGCTGTTTTGTCTTCATGATGCTCTCTTGGAGATTCTGCAAATCGTGC AGTTGTGCTGAAAGAGTACCCTTAAAAGATGACTCCAAAATGTCTAGCACATTCGATATGCCCT TCAAGTATAATGAAAATTTCAGAAGTAAACTCAATCTCTCTTTCAAGCCTAAAATGTCCATATC ATAAATTCCCTGCAGTTCAGCAATGATGTTTTTGAATGTCCTCTTGCACCAATCCTTCACTACC ACTTCATCCAACAAAAATGCTATGACAGGATCACTGGAAACAGCAGTTTCATCCATACATACAT CAGTGATGT
Genomic sequence	AATGGCCTGTTTTGCAACCACCATATCAGAGCCTCCATGTAAAAAGAGCAAGTCAACAGCAGAA CGAAGATTTTTGAAGGGATAGTAACCCAGCATCTGATCAGTATTTGACCCCAAAAATGATGCAC TATCCTTCTGCAAGGATGCAATTTCTAATCCCTCTACAATTTCATTCCTAAACCCCTCCTCCAG ATCAAGATTATTCAATGCATCTTCAATAAATAGAGACCCATCATGTCCTTGTGACTCCACAGAT TGATTTATTGCATCAGGCCATGCTCGTCTAATTGCTTCTGATTTTCGTATCCGAACAACTGATG ACCATGATGAACCATCAGTAAAACGTGACCTCACACCTTCCAAAAATTGATGTCTTGTACACCA AATAATAACATCCATATGCTGGGTAGAACAAAAACAAAAATTAAACACCAGGATGAAGATTGCA ATGAGCTACTCTAATTTTTGGAGCTATATTAGAGTAAGTATTTAGTTACTAACAGATTGGGTTA GTTAACAGTTAGTCTCGCTATATATAGAGATGACTGATAGTTAGTCTTTCTTTTTCTTGATCAA TGAGAAATCAGAGTTTATCAAACTCTATTACAGACAGACAGATAGAGGAAGACTAAATCCAGCC AATCACTCTTTATAAGCAATTAATTAAAGTGCAAGGCCAACTATCAGTCATTTCTACAAGCATC CCTCTTACACACTTCATCATAGCGAACAACAACAAAGGTTACCCATACTCATCCACACTTTTGA AGATTGGGTATAAAGCCCCTTCCAACAAAAATCACACCGAATCATTTGAAGAACACAATGCACA AAAACAATAGCAATACCATTAAAAGAATAGATAATATGTACAAATTTATCATGATGACTTGACA GCACATCAGCCAAGCAAGAAATGGGTCAAAAATCAAATATTAATGCAAAAATCATAACAGTTAT GCAATAACTCCATTGAACATGAATTCTAGCAAAACCAAACCTGTTTTGTCTTCATGATGCTCTC TTGGAGATTCTGCAAATCGTGCAGTTGTGCTGAAAGAGTACCCTTAAAAGATGACTCCAAAATG TCTAGCACATTCGATATGCCCTTCAAGTATAATGAAAATTTCAGAAGTAAACTCAATCTCTCTT TCAAGCCTAAAATGTCCATATCATCTAGTGAAAATTGTCATGGAGTGCAAACATAATTATGGAA AAAACGTCATACTAATAATTAGCTTTAGATATCAACAATAAAACAAGGTTTAAGGATACAAATT CCCTGCAGTTCAGCAATGATGTTTTTGAATGTCCTCTTGCACCAATCCTTCACTACCACTTCAT CCAACAAAAATGCTATGACAGGATCACTGGAAACAGCAGTTTCATCCATACATACATCAGTGAT GT

			
						 Conservation Information
					
						Conserved circRNAs
						
							ghi_circ_003444 gra_circ_000873 gar_circ_000746 ghi_circ_003443 
ghi_circ_001551 ghi_circ_001550 						
					
						PMCS
						0.47687273

Conservation Information
Conserved circRNAs	ghi_circ_003444 gra_circ_000873 gar_circ_000746 ghi_circ_003443 ghi_circ_001551 ghi_circ_001550
PMCS	0.47687273

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							2579248-2579212(-)
						
						Potential amino acid sequence
						
							MDETAVSSDPVIAFLLDEVVVKDWCKRTFKNIIAELQGIYDMDILGLKERLSLLLKFSLYLKGI
SNVLDILESSFKGTLSAQLHDLQNLQESIMKTKQHMDVIIWCTRHQFLEGVRSRFTDGSSWSSV
VRIRKSEAIRRAWPDAINQSVESQGHDGSLFIEDALNNLDLEEGFRNEIVEGLEIASLQKDSAS
FLGSNTDQMLGYYPFKNLRSAVDLLFLHGGSDMVVAKQAITSLMYVWMKLLFPVILS*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						cytoplasmic male sterility

Functional Information
Coding potential	Y
Potential coding position	2579248-2579212(-)
Potential amino acid sequence	MDETAVSSDPVIAFLLDEVVVKDWCKRTFKNIIAELQGIYDMDILGLKERLSLLLKFSLYLKGI SNVLDILESSFKGTLSAQLHDLQNLQESIMKTKQHMDVIIWCTRHQFLEGVRSRFTDGSSWSSV VRIRKSEAIRRAWPDAINQSVESQGHDGSLFIEDALNNLDLEEGFRNEIVEGLEIASLQKDSAS FLGSNTDQMLGYYPFKNLRSAVDLLFLHGGSDMVVAKQAITSLMYVWMKLLFPVILS*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	cytoplasmic male sterility

			
						 Other Information
					
						References
						Zhao et al., 2017a; Chen et al., 2018

circRNA

Detail information of GLYMA_11G035800_circ_g.2

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