Plant Circular RNA Database

Detail information of BGIOSGA013510_circ_g.3

			
						 General Information
					
						CircRNA Name
						BGIOSGA013510_circ_g.3
					
						ID in PlantcircBase
						osi_circ_005081
					
						Alias
						3:34220472|34220857
					
						Organism
						
							Oryza sativa ssp. indica						
					
						Position
						
							chr3: 34220472-34220857  JBrowse»						
					
						Reference genome
						
							Oryza_indica.ASM465v1.42						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							BGIOSGA013510						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							BGIOSGA013510_circ_g.1 BGIOSGA013510_circ_g.2 BGIOSGA013510_circ_g.4 						
					
						Support reads
						NA
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						BGIOSGA013510-TA:1

General Information
CircRNA Name	BGIOSGA013510_circ_g.3
ID in PlantcircBase	osi_circ_005081
Alias	3:34220472\|34220857
Organism	Oryza sativa ssp. indica
Position	chr3: 34220472-34220857 JBrowse»
Reference genome	Oryza_indica.ASM465v1.42
Type	e-circRNA
Identification method	find_circ
Parent gene	BGIOSGA013510
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	BGIOSGA013510_circ_g.1 BGIOSGA013510_circ_g.2 BGIOSGA013510_circ_g.4
Support reads	NA
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	BGIOSGA013510-TA:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: TCCGTCAACATCCTCCGCT
 R: CCATCACCGAGTCGAAGAAGT
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: TCCGTCAACATCCTCCGCT R: CCATCACCGAGTCGAAGAAGT PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TGGGGATCATGGAGCAGGAGGCGTGGCGCCCCCAGCGCGGCCTCCCCGAGCGCTCCGTCAACAT
CCTCCGCTCCTGGCTCTTCGAACACTTCCTCCACCCgtggacaggaggtacaaccactactgcg
accagatgcagatggtggtgaacttcttcgactcggtgatggggttcggggcggcgacgccgta
cacggcgc

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GTGGACAGGAGGTACAACCACTACTGCGACCAGATGCAGATGGTGGTGAACTTCTTCGACTCGG
TGATGGGGTTCGGGGCGGCGACGCCGTACACGGCGCTGGCGCAGAAGGCCATGTCGAGGCACTT
CCGGTGCCTCAAGGACGCGATCGCGGCGCAGCTGAGGGGGACGTGCGAGGCGCTGGGGGAGAAG
GACGCCGGCACGGGGTCGGGGCTGACCAAGGGGGAGACGCCGCGGCTGCGGGCCATCGACCAGA
GCCTCCGGCAGCAGCGCGCCTTCCACCACATGGGGATCATGGAGCAGGAGGCGTGGCGCCCCCA
GCGCGGCCTCCCCGAGCGCTCCGTCAACATCCTCCGCTCCTGGCTCTTCGAACACTTCCTCCAC
CC

Sequences
Splice junction sequence	TGGGGATCATGGAGCAGGAGGCGTGGCGCCCCCAGCGCGGCCTCCCCGAGCGCTCCGTCAACAT CCTCCGCTCCTGGCTCTTCGAACACTTCCTCCACCCgtggacaggaggtacaaccactactgcg accagatgcagatggtggtgaacttcttcgactcggtgatggggttcggggcggcgacgccgta cacggcgc
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GTGGACAGGAGGTACAACCACTACTGCGACCAGATGCAGATGGTGGTGAACTTCTTCGACTCGG TGATGGGGTTCGGGGCGGCGACGCCGTACACGGCGCTGGCGCAGAAGGCCATGTCGAGGCACTT CCGGTGCCTCAAGGACGCGATCGCGGCGCAGCTGAGGGGGACGTGCGAGGCGCTGGGGGAGAAG GACGCCGGCACGGGGTCGGGGCTGACCAAGGGGGAGACGCCGCGGCTGCGGGCCATCGACCAGA GCCTCCGGCAGCAGCGCGCCTTCCACCACATGGGGATCATGGAGCAGGAGGCGTGGCGCCCCCA GCGCGGCCTCCCCGAGCGCTCCGTCAACATCCTCCGCTCCTGGCTCTTCGAACACTTCCTCCAC CC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osa_circ_021656* zma_circ_006918 zma_circ_000876 ecr_circ_000321 
osa_circ_008523 						
					
						PMCS

Conservation Information
Conserved circRNAs	osa_circ_021656* zma_circ_006918 zma_circ_000876 ecr_circ_000321 osa_circ_008523
PMCS

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							34220506-34220517(+)
						
						Potential amino acid sequence
						
							MQMVVNFFDSVMGFGAATPYTALAQKAMSRHFRCLKDAIAAQLRGTCEALGEKDAGTGSGLTKG
ETPRLRAIDQSLRQQRAFHHMGIMEQEAWRPQRGLPERSVNILRSWLFEHFLHPWTGGTTTTAT
RCRWW*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						leaf senescence (regulating to parental gene)(validated)

Functional Information
Coding potential	Y
Potential coding position	34220506-34220517(+)
Potential amino acid sequence	MQMVVNFFDSVMGFGAATPYTALAQKAMSRHFRCLKDAIAAQLRGTCEALGEKDAGTGSGLTKG ETPRLRAIDQSLRQQRAFHHMGIMEQEAWRPQRGLPERSVNILRSWLFEHFLHPWTGGTTTTAT RCRWW*(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	leaf senescence (regulating to parental gene)(validated)

			
						 Other Information
					
						References
						Huang et al., 2021

circRNA

Detail information of BGIOSGA013510_circ_g.3

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