Plant Circular RNA Database

Detail information of GLYMA_08G237500_circ_g.1

			
						 General Information
					
						CircRNA Name
						GLYMA_08G237500_circ_g.1
					
						ID in PlantcircBase
						gma_circ_002126
					
						Alias
						Gm08circRNA235
					
						Organism
						
							Glycine max						
					
						Position
						
							chr8: 20205875-20206460  JBrowse»						
					
						Reference genome
						
							v2.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						Tophat, CIRI
					
						Parent gene
						
							GLYMA_08G237500						
					
						Parent gene annotation
						
							hypothetical protein
							
						Parent gene strand
						-
					
						Alternative splicing
						
							GLYMA_08G237500_circ_g.2 GLYMA_08G237500_circ_g.3 GLYMA_08G237500_circ_g.4 GLYMA_08G237500_circ_g.5 GLYMA_08G237500_circ_g.6 						
					
						Support reads
						NA
					
						Tissues
						leaf, root, stem
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						KRH44887:3
KRH44886:3

General Information
CircRNA Name	GLYMA_08G237500_circ_g.1
ID in PlantcircBase	gma_circ_002126
Alias	Gm08circRNA235
Organism	Glycine max
Position	chr8: 20205875-20206460 JBrowse»
Reference genome	v2.0.38
Type	e-circRNA
Identification method	Tophat, CIRI
Parent gene	GLYMA_08G237500
Parent gene annotation	hypothetical protein
Parent gene strand	-
Alternative splicing	GLYMA_08G237500_circ_g.2 GLYMA_08G237500_circ_g.3 GLYMA_08G237500_circ_g.4 GLYMA_08G237500_circ_g.5 GLYMA_08G237500_circ_g.6
Support reads	NA
Tissues	leaf, root, stem
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	KRH44887:3 KRH44886:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: GGTAGTAGCTCATGGTGGTC
 R: ACCCATACCCTCAAGCAGTT
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: GGTAGTAGCTCATGGTGGTC R: ACCCATACCCTCAAGCAGTT PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GCTCTTCCTCTGTCAGAAGGACACCCATACCCTCAAGCAGTTGTTTATCTAGTACATCAATAAT
ATCTTGCTGGAAAATTTTGGAATGCCCTTTTCTCACcttcgttatcttttcaagatcatcactt
ccaccatcagttatatcatcaccaaatataatacgttcagcacatcgaccaccatgagctacta
ccatttgc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 414
Transcript exons: 20205875-20206051,20206148-20206264,20206341-20206460
CTTCGTTATCTTTTCAAGATCATCACTTCCACCATCAGTTATATCATCACCAAATATAATACGT
TCAGCACATCGACCACCATGAGCTACTACCATTTGCATCATCATGTAACCAAATGTTGTATAGC
CTTGGTCTACCATATCTTCTCGAGGATAAAATACAGATATTGCAGTTTCCTTACCACCAGGCAG
GAGCTGTGAAAATGCATGCCAGTCAAACCGAGGAAATAAGTGAGCTAGCACTACATGACCAGCT
TCATGAACAGCAAGCAGTCTCTTCTTTTCAAAGGATAACCTTTGTTCACATTTTTGTTGCTCTT
CCTCTGTCAGAAGGACACCCATACCCTCAAGCAGTTGTTTATCTAGTACATCAATAATATCTTG
CTGGAAAATTTTGGAATGCCCTTTTCTCAC
						
						Genomic sequence
						
							CTTCGTTATCTTTTCAAGATCATCACTTCCACCATCAGTTATATCATCACCAAATATAATACGT
TCAGCACATCGACCACCATGAGCTACTACCATTTGCATCATCATGTAACCAAATGTTGTATAGC
CTTGGTCTACCATATCTTCTCGAGGATAAAATACAGATATTGCAGTTTCCTGCATAATTAGAAC
ATAAATTCCATGTAAAGACAAAATAATAATAGTAATAATCAAATATCATCATTTTGCATATTGC
AGTAACCTGCGGCATACCTTACCACCAGGCAGGAGCTGTGAAAATGCATGCCAGTCAAACCGAG
GAAATAAGTGAGCTAGCACTACATGACCAGCTTCATGAACAGCAAGCAGTCTCTTCTTTTCAAA
GGATAACTATAGTACAGAAACAAATTCAATACAAAATGCTTTGCCAGACCCACAGTAAGAAAAC
TAACGAAGACAGACATACCCTTTGTTCACATTTTTGTTGCTCTTCCTCTGTCAGAAGGACACCC
ATACCCTCAAGCAGTTGTTTATCTAGTACATCAATAATATCTTGCTGGAAAATTTTGGAATGCC
CTTTTCTCAC

Sequences
Splice junction sequence	GCTCTTCCTCTGTCAGAAGGACACCCATACCCTCAAGCAGTTGTTTATCTAGTACATCAATAAT ATCTTGCTGGAAAATTTTGGAATGCCCTTTTCTCACcttcgttatcttttcaagatcatcactt ccaccatcagttatatcatcaccaaatataatacgttcagcacatcgaccaccatgagctacta ccatttgc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 414 Transcript exons: 20205875-20206051,20206148-20206264,20206341-20206460 CTTCGTTATCTTTTCAAGATCATCACTTCCACCATCAGTTATATCATCACCAAATATAATACGT TCAGCACATCGACCACCATGAGCTACTACCATTTGCATCATCATGTAACCAAATGTTGTATAGC CTTGGTCTACCATATCTTCTCGAGGATAAAATACAGATATTGCAGTTTCCTTACCACCAGGCAG GAGCTGTGAAAATGCATGCCAGTCAAACCGAGGAAATAAGTGAGCTAGCACTACATGACCAGCT TCATGAACAGCAAGCAGTCTCTTCTTTTCAAAGGATAACCTTTGTTCACATTTTTGTTGCTCTT CCTCTGTCAGAAGGACACCCATACCCTCAAGCAGTTGTTTATCTAGTACATCAATAATATCTTG CTGGAAAATTTTGGAATGCCCTTTTCTCAC
Genomic sequence	CTTCGTTATCTTTTCAAGATCATCACTTCCACCATCAGTTATATCATCACCAAATATAATACGT TCAGCACATCGACCACCATGAGCTACTACCATTTGCATCATCATGTAACCAAATGTTGTATAGC CTTGGTCTACCATATCTTCTCGAGGATAAAATACAGATATTGCAGTTTCCTGCATAATTAGAAC ATAAATTCCATGTAAAGACAAAATAATAATAGTAATAATCAAATATCATCATTTTGCATATTGC AGTAACCTGCGGCATACCTTACCACCAGGCAGGAGCTGTGAAAATGCATGCCAGTCAAACCGAG GAAATAAGTGAGCTAGCACTACATGACCAGCTTCATGAACAGCAAGCAGTCTCTTCTTTTCAAA GGATAACTATAGTACAGAAACAAATTCAATACAAAATGCTTTGCCAGACCCACAGTAAGAAAAC TAACGAAGACAGACATACCCTTTGTTCACATTTTTGTTGCTCTTCCTCTGTCAGAAGGACACCC ATACCCTCAAGCAGTTGTTTATCTAGTACATCAATAATATCTTGCTGGAAAATTTTGGAATGCC CTTTTCTCAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.928386348

Conservation Information
Conserved circRNAs	NA
PMCS	0.928386348

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							20206447-20206168(+)
20206390-20205877(-)
						
						Potential amino acid sequence
						
							MPFSHLRYLFKIITSTISYIITKYNTFSTSTTMSYYHLHHHVTKCCIALVYHIFSRIKYRYCSF
LTTRQEL*(+)
MGVLLTEEEQQKCEQRLSFEKKRLLAVHEAGHVVLAHLFPRFDWHAFSQLLPGGKETAISVFYP
REDMVDQGYTTFGYMMMQMVVAHGGRCAERIIFGDDITDGGSDDLEKITKVRKGHSKIFQQDII
DVLDKQLLEGMGVLLTEEEQQKCEQRLSFEKKRLLAVHEAGHVVLAHLFPRFDWHAFSQLLPGG
KETAISVFYPREDMVDQGYTTFGYMMMQMVVAHGGRCAERIIFGDDITDGGSDDLEKITKVRKG
HSKIFQQDIIDVLDKQLLEGMGVLLTEEEQQKCEQRLSFEKKRLLAVHEAGHVVLAHLFPRFDW
HAFSQLLPGGKETAISVFYPREDMVDQGYTTFGYMMMQMVVAHGGRCAERIIFGDDITDGGSDD
LEKITKVRKGHSKIFQQDIIDVLDKQLLEGMGVLLTEEEQQKCEQRLSFEKKRLLAVHEAGHVV
LAHLFPRFDWHAFSQLLPGGKETAISVFYPREDMVDQGYTTFGYMMMQMVVAHGGRCAERIIFG
DDITDGGSDDLEKITK(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	20206447-20206168(+) 20206390-20205877(-)
Potential amino acid sequence	MPFSHLRYLFKIITSTISYIITKYNTFSTSTTMSYYHLHHHVTKCCIALVYHIFSRIKYRYCSF LTTRQEL*(+) MGVLLTEEEQQKCEQRLSFEKKRLLAVHEAGHVVLAHLFPRFDWHAFSQLLPGGKETAISVFYP REDMVDQGYTTFGYMMMQMVVAHGGRCAERIIFGDDITDGGSDDLEKITKVRKGHSKIFQQDII DVLDKQLLEGMGVLLTEEEQQKCEQRLSFEKKRLLAVHEAGHVVLAHLFPRFDWHAFSQLLPGG KETAISVFYPREDMVDQGYTTFGYMMMQMVVAHGGRCAERIIFGDDITDGGSDDLEKITKVRKG HSKIFQQDIIDVLDKQLLEGMGVLLTEEEQQKCEQRLSFEKKRLLAVHEAGHVVLAHLFPRFDW HAFSQLLPGGKETAISVFYPREDMVDQGYTTFGYMMMQMVVAHGGRCAERIIFGDDITDGGSDD LEKITKVRKGHSKIFQQDIIDVLDKQLLEGMGVLLTEEEQQKCEQRLSFEKKRLLAVHEAGHVV LAHLFPRFDWHAFSQLLPGGKETAISVFYPREDMVDQGYTTFGYMMMQMVVAHGGRCAERIIFG DDITDGGSDDLEKITK(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017a

circRNA

Detail information of GLYMA_08G237500_circ_g.1

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