Plant Circular RNA Database

Detail information of Os01g0673500_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os01g0673500_circ_g.2
					
						ID in PlantcircBase
						osa_circ_003181
					
						Alias
						Os01circ19845/Os_ciR2734
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 27661012-27662057  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, SMALT, Segemehl, find_circ
					
						Parent gene
						
							Os01g0673500						
					
						Parent gene annotation
						
							Similar to predicted protein. (Os01t0673500-01);Similar to Katan
in p60 ATPase-containing subunit A1 (EC 3.6.4.3) (Katanin p60 su
bunit A1) (p60 katanin). Splice isoform 2. (Os01t0673500-02);Sim
ilar to predicted protein. (Os01t0673500-03)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os01g0673500_circ_g.1 Os01g0673500_circ_g.3 Os01g0673500_circ_g.4 Os01g0673500_circ_g.5 						
					
						Support reads
						2/4/2
					
						Tissues
						leaf/root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0673500-03:2
Os01t0673500-02:2
Os01t0673500-01:2

General Information
CircRNA Name	Os01g0673500_circ_g.2
ID in PlantcircBase	osa_circ_003181
Alias	Os01circ19845/Os_ciR2734
Organism	Oryza sativa
Position	chr1: 27661012-27662057 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, SMALT, Segemehl, find_circ
Parent gene	Os01g0673500
Parent gene annotation	Similar to predicted protein. (Os01t0673500-01);Similar to Katan in p60 ATPase-containing subunit A1 (EC 3.6.4.3) (Katanin p60 su bunit A1) (p60 katanin). Splice isoform 2. (Os01t0673500-02);Sim ilar to predicted protein. (Os01t0673500-03)
Parent gene strand	-
Alternative splicing	Os01g0673500_circ_g.1 Os01g0673500_circ_g.3 Os01g0673500_circ_g.4 Os01g0673500_circ_g.5
Support reads	2/4/2
Tissues	leaf/root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0673500-03:2 Os01t0673500-02:2 Os01t0673500-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GTTTCTCCAAACGGCGCAACATTGCTGCATCCAGTTCCCATGGCAAATTTGTGGCGGCTAGAAC
AAAAACGAGATCATCTGTCTTAGTTAACCCATCCATcatcctcaggcacttcttcttgccttcc
ttcaagaaccgacattaaccttctaaggggttgcattgccgcctccttgcacacaaggcgtata
tcagagcc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 325
Transcript exons: 27661012-27661234,27661956-27662057
CATCCTCAGGCACTTCTTCTTGCCTTCCTTCAAGAACCGACATTAACCTTCTAAGGGGTTGCAT
TGCCGCCTCCTTGCACACAAGGCGTATATCAGAGCCTGAATATCCTTCAGTTTTCTCCACAAGA
ACATCATATGGGATATTCATTGTGCCAGGAACAGATGGCAAGAGCTCTTCAAACATGGCATGTC
TAGCTTCTTGCTCTGGTAAGGGAACAAGAATACGTTTCTCCAAACGGCGCAACATTGCTGCATC
CAGTTCCCATGGCAAATTTGTGGCGGCTAGAACAAAAACGAGATCATCTGTCTTAGTTAACCCA
TCCAT
						
						Genomic sequence
						
							CATCCTCAGGCACTTCTTCTTGCCTTCCTTCAAGAACCGACATTAACCTTCTAAGGGGTTGCAT
TGCCGCCTCCTTGCACACAAGGCGTATATCAGAGCCTGAATATCCTTCAGTTTTCTCCACAAGA
ACATCATATGGGATATTCATTGTGCCAGGAACAGATGGCAAGAGCTCTTCAAACATGGCATGTC
TAGCTTCTTGCTCTGGTAAGGGAACAAGAATCTAATTGCAAGTTAAGGATGGATTTCCCTTTTT
ATGTGGAAAAATCAAGTGTGGTCCTATATACAAACTCCAGTATCGCAATAAGAAAAGATACAAG
AATCGAGATTGAATTCTTTAACAGTTTTTAGTACAAACAATAACTTCAGTAAGAAGCCTATGAT
GTGTCAGTTACAACTACAAATAGATTTAAGGCAAGCATTGGAAATAGGCAACATTTACAACTAC
AAATAGATTTAAGGCAAGCATTGGAAATAGGCTACATAAATGTTCATCATCAAACATTCTCTTT
ACTACATAATAAGAGAATCACTTATTCTCAATCTGAAATTCAAATCAGTAGTTCCTCACATAAG
AAAATGATCTTTGGTATTGTTACTTCTGGTTAAACATATTTGAGATTTGAATTTTTTTTCATGG
TCTTCAAGGTGCGATTGTTACCATGATTTTATTAGAATAGGTTTATAAAATCTAATAAGTTTAT
GAGATGTTGGAAGTACTTTAAAACAAATCAAAGCATATCATATTTATGTTTTTGAACTAAGTTT
TTAAAGTTCATCGATAGTCAAGGTTTCAAAAGTTTAACCAGATTTTGTCCAAAACATCAAGCAT
TTGCGGACAAAACAAGGGAGTTTACCTAATAGCAAAACTGGCAATTACGGAGTAGTATTTAGGA
GCACTCGTGAACAAAGAAATTACAAGATGGTTTGAGAAAAAAGGATACACGTTTCTCCAAACGG
CGCAACATTGCTGCATCCAGTTCCCATGGCAAATTTGTGGCGGCTAGAACAAAAACGAGATCAT
CTGTCTTAGTTAACCCATCCAT

Sequences
Splice junction sequence	GTTTCTCCAAACGGCGCAACATTGCTGCATCCAGTTCCCATGGCAAATTTGTGGCGGCTAGAAC AAAAACGAGATCATCTGTCTTAGTTAACCCATCCATcatcctcaggcacttcttcttgccttcc ttcaagaaccgacattaaccttctaaggggttgcattgccgcctccttgcacacaaggcgtata tcagagcc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 325 Transcript exons: 27661012-27661234,27661956-27662057 CATCCTCAGGCACTTCTTCTTGCCTTCCTTCAAGAACCGACATTAACCTTCTAAGGGGTTGCAT TGCCGCCTCCTTGCACACAAGGCGTATATCAGAGCCTGAATATCCTTCAGTTTTCTCCACAAGA ACATCATATGGGATATTCATTGTGCCAGGAACAGATGGCAAGAGCTCTTCAAACATGGCATGTC TAGCTTCTTGCTCTGGTAAGGGAACAAGAATACGTTTCTCCAAACGGCGCAACATTGCTGCATC CAGTTCCCATGGCAAATTTGTGGCGGCTAGAACAAAAACGAGATCATCTGTCTTAGTTAACCCA TCCAT
Genomic sequence	CATCCTCAGGCACTTCTTCTTGCCTTCCTTCAAGAACCGACATTAACCTTCTAAGGGGTTGCAT TGCCGCCTCCTTGCACACAAGGCGTATATCAGAGCCTGAATATCCTTCAGTTTTCTCCACAAGA ACATCATATGGGATATTCATTGTGCCAGGAACAGATGGCAAGAGCTCTTCAAACATGGCATGTC TAGCTTCTTGCTCTGGTAAGGGAACAAGAATCTAATTGCAAGTTAAGGATGGATTTCCCTTTTT ATGTGGAAAAATCAAGTGTGGTCCTATATACAAACTCCAGTATCGCAATAAGAAAAGATACAAG AATCGAGATTGAATTCTTTAACAGTTTTTAGTACAAACAATAACTTCAGTAAGAAGCCTATGAT GTGTCAGTTACAACTACAAATAGATTTAAGGCAAGCATTGGAAATAGGCAACATTTACAACTAC AAATAGATTTAAGGCAAGCATTGGAAATAGGCTACATAAATGTTCATCATCAAACATTCTCTTT ACTACATAATAAGAGAATCACTTATTCTCAATCTGAAATTCAAATCAGTAGTTCCTCACATAAG AAAATGATCTTTGGTATTGTTACTTCTGGTTAAACATATTTGAGATTTGAATTTTTTTTCATGG TCTTCAAGGTGCGATTGTTACCATGATTTTATTAGAATAGGTTTATAAAATCTAATAAGTTTAT GAGATGTTGGAAGTACTTTAAAACAAATCAAAGCATATCATATTTATGTTTTTGAACTAAGTTT TTAAAGTTCATCGATAGTCAAGGTTTCAAAAGTTTAACCAGATTTTGTCCAAAACATCAAGCAT TTGCGGACAAAACAAGGGAGTTTACCTAATAGCAAAACTGGCAATTACGGAGTAGTATTTAGGA GCACTCGTGAACAAAGAAATTACAAGATGGTTTGAGAAAAAAGGATACACGTTTCTCCAAACGG CGCAACATTGCTGCATCCAGTTCCCATGGCAAATTTGTGGCGGCTAGAACAAAAACGAGATCAT CTGTCTTAGTTAACCCATCCAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_010546 						
					
						PMCS
						0.468616108

Conservation Information
Conserved circRNAs	osi_circ_010546
PMCS	0.468616108

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							27661195-27661054(+)
27662059-27662043(-)
27661016-27662043(-)
						
						Potential amino acid sequence
						
							MACLASCSGKGTRIRFSKRRNIAASSSHGKFVAARTKTRSSVLVNPSIILRHFFLPSFKNRH*(
+)
MDGLTKTDDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEQEARHAMFEELLPSVPGTMNIPY
DVLVEKTEGYSGSDIRLVCKEAAMQPLRRLMSVLEGRQEEVPEDDGWVN*(-)
MMDGLTKTDDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEQEARHAMFEELLPSVPGTMNIP
YDVLVEKTEGYSGSDIRLVCKEAAMQPLRRLMSVLEGRQEEVPEDDGWVN*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	27661195-27661054(+) 27662059-27662043(-) 27661016-27662043(-)
Potential amino acid sequence	MACLASCSGKGTRIRFSKRRNIAASSSHGKFVAARTKTRSSVLVNPSIILRHFFLPSFKNRH( +) MDGLTKTDDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEQEARHAMFEELLPSVPGTMNIPY DVLVEKTEGYSGSDIRLVCKEAAMQPLRRLMSVLEGRQEEVPEDDGWVN(-) MMDGLTKTDDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEQEARHAMFEELLPSVPGTMNIP YDVLVEKTEGYSGSDIRLVCKEAAMQPLRRLMSVLEGRQEEVPEDDGWVN*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015;Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os01g0673500_circ_g.2

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