Plant Circular RNA Database

Detail information of Os01g0510100_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os01g0510100_circ_g.4
					
						ID in PlantcircBase
						osa_circ_001889
					
						Alias
						Os_ciR3499
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 17905649-17907588  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os01g0510100						
					
						Parent gene annotation
						
							MAP kinase kinase 1. (Os01t0510100-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os01g0510100_circ_g.2 Os01g0510100_circ_g.3 Os01g0510100_circ_g.5 Os01g0510100_circ_g.6 						
					
						Support reads
						2/1
					
						Tissues
						root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0510100-01:5

General Information
CircRNA Name	Os01g0510100_circ_g.4
ID in PlantcircBase	osa_circ_001889
Alias	Os_ciR3499
Organism	Oryza sativa
Position	chr1: 17905649-17907588 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os01g0510100
Parent gene annotation	MAP kinase kinase 1. (Os01t0510100-01)
Parent gene strand	+
Alternative splicing	Os01g0510100_circ_g.2 Os01g0510100_circ_g.3 Os01g0510100_circ_g.5 Os01g0510100_circ_g.6
Support reads	2/1
Tissues	root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0510100-01:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TAAGCTTCTACGAATTATTAGAAGCAATTGTTGACCAGCCACCACCCTCTGCACCAGCGGACCA
GTTCTCTCCAGAATTTTGTGCATTTATCTCCTCCTGgaccgcgagtggtacattcaaggatggt
gaactgcggcttaatcaaagaggtttgcagcttatatccgaggaaactgcagatgaacctgtga
gttcctgg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 855
Transcript exons: 17905649-17905736,17906086-17906223,17906711-17906935,17
907067-17907249,17907368-17907588
GACCGCGAGTGGTACATTCAAGGATGGTGAACTGCGGCTTAATCAAAGAGGTTTGCAGCTTATA
TCCGAGGAAACTGCAGATGAACCTCAGTCAACAAACCTGAAGGTGGAAGATGTTCAGTTGTCAA
TGGATGACCTTGAGATGATTCAAGTCATTGGTAAGGGGAGTGGTGGTATCGTCCAACTAGTTCG
GCACAAGTGGGTGGGGACATTATATGCCTTGAAGGGCATACAAATGAACATTCAAGAGGCAGTT
CGCAAACAAATAGTACAAGAGCTCAAAATAAATCAAGCAACACAGAACGCACATATAGTCCTTT
GCCACCAATCTTTCTACCATAATGGTGTAATATATCTTGTTCTAGAATACATGGACCGTGGATC
TCTTGCAGATATCATTAAACAAGTCAAAACAATTCTGGAGCCATACCTGGCAGTACTTTGCAAA
CAGGTTTTGGAGGGTCTACTGTATCTTCATCATGAAAGGCATGTGATTCACAGGGATATAAAGC
CATCTAACTTGTTAGTTAACCGTAAAGGTGAAGTAAAGATTACCGATTTTGGGGTAAGTGCGGT
GCTAGCAAGTTCAATGGGTCAGCGGGATACGTTTGTTGGAACCTACAACTATATGGCGCCTGAG
CGTATTAGTGGAAGCTCCTATGACTACAAGAGTGACATATGGAGTTTGGGCTTAGTAATACTCG
AGTGTGCCATTGGTCGGTTCCCCTATATACCTTCAGAAGGGGAAGGTTGGTTAAGCTTCTACGA
ATTATTAGAAGCAATTGTTGACCAGCCACCACCCTCTGCACCAGCGGACCAGTTCTCTCCAGAA
TTTTGTGCATTTATCTCCTCCTG
						
					

						Genomic sequence
						
							GACCGCGAGTGGTACATTCAAGGATGGTGAACTGCGGCTTAATCAAAGAGGTTTGCAGCTTATA
TCCGAGGAAACTGCAGATGAACCTGTGAGTTCCTGGTGCCATGGCTACTTTTTTTGTTTGTTCA
TATCTCCCTACTGGGTTCATGTAAACTGCTTTATCGTACTCCTTATGGTGACATGTAGGTTAGT
GTAGAAGCACATGTACAGGTTGACTAGTTCAGTCAAACCTTATCATGCTTCTGTTGATTTTCAT
TTGGCTGATTGAATGCAATACTCACTTGCAATGCATGGGATCCATGCCACTCATGCATTCTCGC
AATGTATTATGTGCTGCAATAGTGCTAAGGAATCATTGTGTAAACATTTCAGAGTTTATCCATG
GATATAACATTGTTCACATTTCTCAATTTCTGTTTCATCTTTATGTCTTCCAGCAGTCAACAAA
CCTGAAGGTGGAAGATGTTCAGTTGTCAATGGATGACCTTGAGATGATTCAAGTCATTGGTAAG
GGGAGTGGTGGTATCGTCCAACTAGTTCGGCACAAGTGGGTGGGGACATTATATGCCTTGAAGG
TAATTTCCATTACACAAAATCCATCTAGTGTTCCACACTAATTCTTAAATGGAAAATGTATATC
TTGTGGCTGCATCAGGCTGCGAGTCCTTCAGTTGAACTGTTTCTCTATCTTTATGTTTGTAATC
ATGGAAAGCTGGAAGTCAATCTCTCAGTTCCATTTTGAACATGTTTATCATAATCAGATTATTC
TGCATCATAGCATTTGATTGATTTTTTAATCAATTTGGTAGTGTTGGCACCTATTAGTATGTTC
CCTGTTGAAATTAGGTGGGGCATTCTACATAAATGGTGTAGCAGAAGCATCTGTCGTCAGCTTA
CGTGTTGCATGCAATATCTACATTGAACTCTGATTTTCAATTAATATTGATGCTAATGAAGAAC
ATCATGTTATGATGTTCAGATCAATCAAGAATTCTGTGCATTGTCCCTGGTATTCATACTGCTG
TGGCATTTAATTAACTAGCATTGCAATTCTGTCCACAGGGCATACAAATGAACATTCAAGAGGC
AGTTCGCAAACAAATAGTACAAGAGCTCAAAATAAATCAAGCAACACAGAACGCACATATAGTC
CTTTGCCACCAATCTTTCTACCATAATGGTGTAATATATCTTGTTCTAGAATACATGGACCGTG
GATCTCTTGCAGATATCATTAAACAAGTCAAAACAATTCTGGAGCCATACCTGGCAGTACTTTG
CAAACAGGTTAGCATAAACTTAGCCTTTTATTTCTAACTTTCCCTGTTTTCGGAATAAGCTTGG
AATTTTGTCGCATCTATCCAGTTGAAAAAGTATTCTAACGTAAAAAAACATTGCATGATATTTT
GACAACTCAGGTTTTGGAGGGTCTACTGTATCTTCATCATGAAAGGCATGTGATTCACAGGGAT
ATAAAGCCATCTAACTTGTTAGTTAACCGTAAAGGTGAAGTAAAGATTACCGATTTTGGGGTAA
GTGCGGTGCTAGCAAGTTCAATGGGTCAGCGGGATACGTTTGTTGGAACCTACAACTATATGGC
GGTATGTTGAAATAATGACCTGCTTTATTTATGTACCCCTTAGTCTATGTTCAAGACAGTGATC
TGATCTGGAATTCATAAATTTGTTCTCATAGTTTCTACCTTTCTTCCTAGTTCAGCCTGAGCGT
ATTAGTGGAAGCTCCTATGACTACAAGAGTGACATATGGAGTTTGGGCTTAGTAATACTCGAGT
GTGCCATTGGTCGGTTCCCCTATATACCTTCAGAAGGGGAAGGTTGGTTAAGCTTCTACGAATT
ATTAGAAGCAATTGTTGACCAGCCACCACCCTCTGCACCAGCGGACCAGTTCTCTCCAGAATTT
TGTGCATTTATCTCCTCCTG
						
					

			
		

Sequences
Splice junction sequence	TAAGCTTCTACGAATTATTAGAAGCAATTGTTGACCAGCCACCACCCTCTGCACCAGCGGACCA GTTCTCTCCAGAATTTTGTGCATTTATCTCCTCCTGgaccgcgagtggtacattcaaggatggt gaactgcggcttaatcaaagaggtttgcagcttatatccgaggaaactgcagatgaacctgtga gttcctgg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 855 Transcript exons: 17905649-17905736,17906086-17906223,17906711-17906935,17 907067-17907249,17907368-17907588 GACCGCGAGTGGTACATTCAAGGATGGTGAACTGCGGCTTAATCAAAGAGGTTTGCAGCTTATA TCCGAGGAAACTGCAGATGAACCTCAGTCAACAAACCTGAAGGTGGAAGATGTTCAGTTGTCAA TGGATGACCTTGAGATGATTCAAGTCATTGGTAAGGGGAGTGGTGGTATCGTCCAACTAGTTCG GCACAAGTGGGTGGGGACATTATATGCCTTGAAGGGCATACAAATGAACATTCAAGAGGCAGTT CGCAAACAAATAGTACAAGAGCTCAAAATAAATCAAGCAACACAGAACGCACATATAGTCCTTT GCCACCAATCTTTCTACCATAATGGTGTAATATATCTTGTTCTAGAATACATGGACCGTGGATC TCTTGCAGATATCATTAAACAAGTCAAAACAATTCTGGAGCCATACCTGGCAGTACTTTGCAAA CAGGTTTTGGAGGGTCTACTGTATCTTCATCATGAAAGGCATGTGATTCACAGGGATATAAAGC CATCTAACTTGTTAGTTAACCGTAAAGGTGAAGTAAAGATTACCGATTTTGGGGTAAGTGCGGT GCTAGCAAGTTCAATGGGTCAGCGGGATACGTTTGTTGGAACCTACAACTATATGGCGCCTGAG CGTATTAGTGGAAGCTCCTATGACTACAAGAGTGACATATGGAGTTTGGGCTTAGTAATACTCG AGTGTGCCATTGGTCGGTTCCCCTATATACCTTCAGAAGGGGAAGGTTGGTTAAGCTTCTACGA ATTATTAGAAGCAATTGTTGACCAGCCACCACCCTCTGCACCAGCGGACCAGTTCTCTCCAGAA TTTTGTGCATTTATCTCCTCCTG
Genomic sequence	GACCGCGAGTGGTACATTCAAGGATGGTGAACTGCGGCTTAATCAAAGAGGTTTGCAGCTTATA TCCGAGGAAACTGCAGATGAACCTGTGAGTTCCTGGTGCCATGGCTACTTTTTTTGTTTGTTCA TATCTCCCTACTGGGTTCATGTAAACTGCTTTATCGTACTCCTTATGGTGACATGTAGGTTAGT GTAGAAGCACATGTACAGGTTGACTAGTTCAGTCAAACCTTATCATGCTTCTGTTGATTTTCAT TTGGCTGATTGAATGCAATACTCACTTGCAATGCATGGGATCCATGCCACTCATGCATTCTCGC AATGTATTATGTGCTGCAATAGTGCTAAGGAATCATTGTGTAAACATTTCAGAGTTTATCCATG GATATAACATTGTTCACATTTCTCAATTTCTGTTTCATCTTTATGTCTTCCAGCAGTCAACAAA CCTGAAGGTGGAAGATGTTCAGTTGTCAATGGATGACCTTGAGATGATTCAAGTCATTGGTAAG GGGAGTGGTGGTATCGTCCAACTAGTTCGGCACAAGTGGGTGGGGACATTATATGCCTTGAAGG TAATTTCCATTACACAAAATCCATCTAGTGTTCCACACTAATTCTTAAATGGAAAATGTATATC TTGTGGCTGCATCAGGCTGCGAGTCCTTCAGTTGAACTGTTTCTCTATCTTTATGTTTGTAATC ATGGAAAGCTGGAAGTCAATCTCTCAGTTCCATTTTGAACATGTTTATCATAATCAGATTATTC TGCATCATAGCATTTGATTGATTTTTTAATCAATTTGGTAGTGTTGGCACCTATTAGTATGTTC CCTGTTGAAATTAGGTGGGGCATTCTACATAAATGGTGTAGCAGAAGCATCTGTCGTCAGCTTA CGTGTTGCATGCAATATCTACATTGAACTCTGATTTTCAATTAATATTGATGCTAATGAAGAAC ATCATGTTATGATGTTCAGATCAATCAAGAATTCTGTGCATTGTCCCTGGTATTCATACTGCTG TGGCATTTAATTAACTAGCATTGCAATTCTGTCCACAGGGCATACAAATGAACATTCAAGAGGC AGTTCGCAAACAAATAGTACAAGAGCTCAAAATAAATCAAGCAACACAGAACGCACATATAGTC CTTTGCCACCAATCTTTCTACCATAATGGTGTAATATATCTTGTTCTAGAATACATGGACCGTG GATCTCTTGCAGATATCATTAAACAAGTCAAAACAATTCTGGAGCCATACCTGGCAGTACTTTG CAAACAGGTTAGCATAAACTTAGCCTTTTATTTCTAACTTTCCCTGTTTTCGGAATAAGCTTGG AATTTTGTCGCATCTATCCAGTTGAAAAAGTATTCTAACGTAAAAAAACATTGCATGATATTTT GACAACTCAGGTTTTGGAGGGTCTACTGTATCTTCATCATGAAAGGCATGTGATTCACAGGGAT ATAAAGCCATCTAACTTGTTAGTTAACCGTAAAGGTGAAGTAAAGATTACCGATTTTGGGGTAA GTGCGGTGCTAGCAAGTTCAATGGGTCAGCGGGATACGTTTGTTGGAACCTACAACTATATGGC GGTATGTTGAAATAATGACCTGCTTTATTTATGTACCCCTTAGTCTATGTTCAAGACAGTGATC TGATCTGGAATTCATAAATTTGTTCTCATAGTTTCTACCTTTCTTCCTAGTTCAGCCTGAGCGT ATTAGTGGAAGCTCCTATGACTACAAGAGTGACATATGGAGTTTGGGCTTAGTAATACTCGAGT GTGCCATTGGTCGGTTCCCCTATATACCTTCAGAAGGGGAAGGTTGGTTAAGCTTCTACGAATT ATTAGAAGCAATTGTTGACCAGCCACCACCCTCTGCACCAGCGGACCAGTTCTCTCCAGAATTT TGTGCATTTATCTCCTCCTG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_002101* osi_circ_009620 						
					
						PMCS
						0.171055799

Conservation Information
Conserved circRNAs	osi_circ_002101* osi_circ_009620
PMCS	0.171055799

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							17906126-17907585(+)
						
						Potential amino acid sequence
						
							MDDLEMIQVIGKGSGGIVQLVRHKWVGTLYALKGIQMNIQEAVRKQIVQELKINQATQNAHIVL
CHQSFYHNGVIYLVLEYMDRGSLADIIKQVKTILEPYLAVLCKQVLEGLLYLHHERHVIHRDIK
PSNLLVNRKGEVKITDFGVSAVLASSMGQRDTFVGTYNYMAPERISGSSYDYKSDIWSLGLVIL
ECAIGRFPYIPSEGEGWLSFYELLEAIVDQPPPSAPADQFSPEFCAFISSWTASGTFKDGELRL
NQRGLQLISEETADEPQSTNLKVEDVQLSMDDLEMIQVIGKGSGGIVQLVRHKWVGTLYALKGI
QMNIQEAVRKQIVQELKINQATQNAHIVLCHQSFYHNGVIYLVLEYMDRGSLADIIKQVKTILE
PYLAVLCKQVLEGLLYLHHERHVIHRDIKPSNLLVNRKGEVKITDFGVSAVLASSMGQRDTFVG
TYNYMAPERISGSSYDYKSDIWSLGLVILECAIGRFPYIPSEGEGWLSFYELLEAIVDQPPPSA
PADQFSPEFCAFISSWTASGTFKDGELRLNQRGLQLISEETADEPQSTNLKVEDVQLSMDDLEM
IQVIGKGSGGIVQLVRHKWVGTLYALKGIQMNIQEAVRKQIVQELKINQATQNAHIVLCHQSFY
HNGVIYLVLEYMDRGSLADIIKQVKTILEPYLAVLCKQVLEGLLYLHHERHVIHRDIKPSNLLV
NRKGEVKITDFGVSAVLASSMGQRDTFVGTYNYMAPERISGSSYDYKSDIWSLGLVILECAIGR
FPYIPSEGEGWLSFYELLEAIVDQPPPSAPADQFSPEFCAFISSWTASGTFKDGELRLNQRGLQ
LISEETADEPQSTNLKVEDVQLSMDDLEMIQVIGKGSGGIVQLVRHKWVGTLYALKGIQMNIQE
AVRKQIVQELKINQATQNAHIVLCHQSFYHNGVIYLVLEYMDRGSLADIIKQVKTILEPYLAVL
CKQVLEGLLYLHHERHVIHRDIKPSNLLVNRKGEVKITDFGVSAVLASSMGQRDTFVGTYNYMA
PERISGSSYDYKSDIWSLGLVILECAIGRFPYIPSEGEGWLSFYELLEAIVDQPPPSAPADQFS
PEFCAFISS(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	17906126-17907585(+)
Potential amino acid sequence	MDDLEMIQVIGKGSGGIVQLVRHKWVGTLYALKGIQMNIQEAVRKQIVQELKINQATQNAHIVL CHQSFYHNGVIYLVLEYMDRGSLADIIKQVKTILEPYLAVLCKQVLEGLLYLHHERHVIHRDIK PSNLLVNRKGEVKITDFGVSAVLASSMGQRDTFVGTYNYMAPERISGSSYDYKSDIWSLGLVIL ECAIGRFPYIPSEGEGWLSFYELLEAIVDQPPPSAPADQFSPEFCAFISSWTASGTFKDGELRL NQRGLQLISEETADEPQSTNLKVEDVQLSMDDLEMIQVIGKGSGGIVQLVRHKWVGTLYALKGI QMNIQEAVRKQIVQELKINQATQNAHIVLCHQSFYHNGVIYLVLEYMDRGSLADIIKQVKTILE PYLAVLCKQVLEGLLYLHHERHVIHRDIKPSNLLVNRKGEVKITDFGVSAVLASSMGQRDTFVG TYNYMAPERISGSSYDYKSDIWSLGLVILECAIGRFPYIPSEGEGWLSFYELLEAIVDQPPPSA PADQFSPEFCAFISSWTASGTFKDGELRLNQRGLQLISEETADEPQSTNLKVEDVQLSMDDLEM IQVIGKGSGGIVQLVRHKWVGTLYALKGIQMNIQEAVRKQIVQELKINQATQNAHIVLCHQSFY HNGVIYLVLEYMDRGSLADIIKQVKTILEPYLAVLCKQVLEGLLYLHHERHVIHRDIKPSNLLV NRKGEVKITDFGVSAVLASSMGQRDTFVGTYNYMAPERISGSSYDYKSDIWSLGLVILECAIGR FPYIPSEGEGWLSFYELLEAIVDQPPPSAPADQFSPEFCAFISSWTASGTFKDGELRLNQRGLQ LISEETADEPQSTNLKVEDVQLSMDDLEMIQVIGKGSGGIVQLVRHKWVGTLYALKGIQMNIQE AVRKQIVQELKINQATQNAHIVLCHQSFYHNGVIYLVLEYMDRGSLADIIKQVKTILEPYLAVL CKQVLEGLLYLHHERHVIHRDIKPSNLLVNRKGEVKITDFGVSAVLASSMGQRDTFVGTYNYMA PERISGSSYDYKSDIWSLGLVILECAIGRFPYIPSEGEGWLSFYELLEAIVDQPPPSAPADQFS PEFCAFISS(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os01g0510100_circ_g.4

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