Plant Circular RNA Database

Detail information of Os06g0218500_circ_g.5

			
						 General Information
					
						CircRNA Name
						Os06g0218500_circ_g.5
					
						ID in PlantcircBase
						osa_circ_030214
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr6: 6091994-6095199  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os06g0218500						
					
						Parent gene annotation
						
							MCM family protein. (Os06t0218500-01);MCM family protein. (Os06t
0218500-02);Non-protein coding transcript. (Os06t0218500-03)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os06g0218500_circ_g.4 Os06g0218500_circ_g.6 						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os06t0218500-02:6
Os06t0218500-01:6

General Information
CircRNA Name	Os06g0218500_circ_g.5
ID in PlantcircBase	osa_circ_030214
Alias	NA
Organism	Oryza sativa
Position	chr6: 6091994-6095199 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os06g0218500
Parent gene annotation	MCM family protein. (Os06t0218500-01);MCM family protein. (Os06t 0218500-02);Non-protein coding transcript. (Os06t0218500-03)
Parent gene strand	-
Alternative splicing	Os06g0218500_circ_g.4 Os06g0218500_circ_g.6
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os06t0218500-02:6 Os06t0218500-01:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CTCACAAAATTGGCAATTAGCATAGGATCAAGGTTTGAACGTACATCCTTGATGTCAGGAGACC
ATTTTGCAGATAATCGACCCGTAACAACAACATCATctatcaaattcatctatgcagcataacc
ctccatcagcaagaacaagagcgccagcctcaagcatccactcccctattatcaaatataaaaa
tggcgttt

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							CTATCAAATTCATCTATGCAGCATAACCCTCCATCAGCAAGAACAAGAGCGCCAGCCTCAAGCA
TCCACTCCCCTATTATCAAATATAAAAATGGCGTTTCAGTTATTATGCAAGAAACTTGCTATGA
AAAATCATTTACTAGTTTCAGATTTTGAAATCGAGGAAATACAAAACACGATATATAATGCTGC
CAGAGAATGGAGATGTGAAACATAGGTACCTCCATCCTTGACTGCTGTGACAGTTAAACCAGCA
CTGGTGCTTCCAAGACCAGTTGTGATCACTGAACGGTTGCTCAATTTAGCAGCAAATTTCAGAA
ACTGGGATTTGCCAGTGCCTAAAATTTAATAGAACACAATTCAGTTAGAGATAATATATATTTC
ATATAGTCTGTTTGTAGTGTCTCTATTAGGAACACCAAATGCACTTTATGAAGCTTGTGGATGT
CAAGCAGTAGTTCTGTACTTTTATGAATCCAAAAGAAGTTATTTGTGCGTCCCTGGGAAGGAAG
ATGCAAGTGGGGCTGGTCAAGCTGACCTACTCTACTTCTTTTTTTTTTCAAATATAGATGCATA
AATCTGACTACAAGTTAGTTCTAGTCTAAATGAATATGCCTAACCTTTTCTTTAAGTCTGTCAC
CAGTCCAGTGGATTGAGCAAACATTCTAGAACACACTGAATAGTAGCTTGGACCTACCTATAGC
GATGTTTCGTTCAGTCTGTTCTTGATAGGATAGGGTAGAGGATGCCTTTCTGTGTGGTGCTGTA
GGATTTCTTGTAACGGGTCAATTACCAAGGTAAAATGCATTGGGACAGAGGACCCCCTTTCCCA
ACCCCTCTTTGATAGTGAAATTGCTCCCTGGAACATCATTGAGCAGCACGTTCGAGGTTGATGA
ACCAAAAAGGATGGATATCTATTCTCTCCACTTTGGCCCTAAGCCTAGTTGTTGTATCTATCCC
CACTGGACTGAATTGAACATTTCTGCCATATACAAGAGGCAGTGTTGGAATTTGTGCAGTGCAC
GAGTAACATTTTGGTATACAGGAAATCATCTTGGATGCACTTTTTCACGCAGGTGCTTCAGCAG
GAGAGGATTGAGTCCATGTACGGACAAGCTAGATGATGGCGTCCAGCTATGGGTCAAGCTAGTT
TTCCAGGAGCTTAGAGAAGGTCTGGCCGAAGATATGGACTAAGCTAGCCTAGCGGTAATCGGTA
GTCCACTGTGTAGCTATTACAGTGAAAGCATATTAACTGAATATGTTGTTTTCTGGAAAAAGGT
TAAACACTAGTTCAACTTATGTACAGAAAGGATGGTTTTGATTTAAGTATATAACAAATGTTAT
GGATATTAGTACCTGGATCACCGACAAGAAGCATATGTGGTTCTCCACGAACCTTTGTTCCTGA
AGCATCAACATGCTGCACCCCGCCAATTAAGGTAAGAGCAACTGAAAATTAAACAGATCATGAA
ACAATACCAACTATAGCAGTTGTTATAGATACTCCAAAAAAGAGTTAAATAGCTTTTCAGAAAC
AATCTCCAACTGGATTTTCAGAGAAAGCTAAACACAAGTGAATTACTCGCAATGATTTAGAATA
CTGGCAAATAGTTCTGTACCTGCAAGCTTCACTGTGAATAACCCATAAATTTGAGGACAAATGC
CTTTCAAGATGGAATTTCTTCCTGTGTACCCAAAAGAACAAAAGAATGCACAGTTTACAGTGCA
ATAATGCATTAAATAAAAGGTAGAAAGCTTCCTAATATTATATTTGGGAACAGGATCAGGGTCC
ATAGTTCAAATGTAAGTCAATGCACAATTTACAGTGCAATAATGCATTAAATAAAAGGTAGAAA
GCTTCCTAATACTTCCTCCATTCCAAATTGATCATATTACATGGGTGCATTAAGACCAAGAAAA
TATAATAACTATCTCATACTACATTTACTCTGACAACAAACTCGATGCATGCACATTCTCCACT
ATTTTTTAGCCAATAGCAAATCAAAACACTTTGTGCATGGATGCATGCATCAATGTTCATTTAC
TTCAATGCACAAATAACAAATAAACTTAATGAATGAACACAAATATGATGATCATTTTAGAATA
TTCTTAAAAATATTATATGATGATCAATTTGGAATGGAGGGAGTATTACATTTGGGAACAGGAT
CAGGGTTTTTGGTCAAATGTAAGTCAATAAGACAATGTAGTACATTGTAATTGCTTCAATATTA
GCTCACAGGATTCAGGCCAAGATCTAGACAGCAACAAAAGGAAAATAAGCTGACATTGGAACAT
AAGCACCTTTCAGTGGAGTAGCTCTGGAAGCTGCCCAGAATTCCTCAAACTTGTTGATAATTTC
AACAGGTATGTCTAGATCAGACTTCAGTTCATTGGTTCTCCTGTTGAGTGCAGTACATAGACAT
TCATAATGCACAGAAGGCAAGACTAGCTGATATCTTTGAACTTGAAACCAAGGAACAGAAAAGG
CGCTAAAATTAAAATACTAAAGTGCTAAAAATATGCCCAACTATGGTTGCATCCAACTACCTTG
GCAGGCAATAAAATGAAAAATAAGAAAAACTACAACGCAATAAACTAGTAGAGTGAAAGTACAG
TGTATGGGAAGCCTTTCCTTCATATGAGTAATGAGACCAGACAATACTACTCTGTTGTCATAAA
CTGCATGTTTCCTAAATGTCTTTTTTAGTTTTTCATCATTTAACTAATAAACACCAAAAGCCAT
TCCATGTACTTTCTGGATCGGCAATTATGTGCCAAAGCCATTGCTGGTATTATTACTGTGATAG
ATTTGGAGAAGCTTAAATGAGCCCCTGCAGGTTCGTTAAAACAACACTATGTAATGAAATACAC
TAAGAATGGCACATTATTGCTGGTATTACTGTGATAGATCTTCAGAAGTATAATGTGTCCCTTC
AGGTTCGTAAGAAGAACACTATGCAATAAAATATGCTAAGAAGAGAAGAAACTATACCTGGTTC
ATTGCATACTCTAAATATGTTCAAAGAATCATAATTTGGAAGATTAAAATTAGAGAACTAATAT
TTGGAGAACACCCTCAGTATAAGTTATCAGTTACCTCACAAAATTGGCAATTAGCATAGGATCA
AGGTTTGAACGTACATCCTTGATGTCAGGAGACCATTTTGCAGATAATCGACCCGTAACAACAA
CATCAT
						
					

			
		

Sequences
Splice junction sequence	CTCACAAAATTGGCAATTAGCATAGGATCAAGGTTTGAACGTACATCCTTGATGTCAGGAGACC ATTTTGCAGATAATCGACCCGTAACAACAACATCATctatcaaattcatctatgcagcataacc ctccatcagcaagaacaagagcgccagcctcaagcatccactcccctattatcaaatataaaaa tggcgttt
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CTATCAAATTCATCTATGCAGCATAACCCTCCATCAGCAAGAACAAGAGCGCCAGCCTCAAGCA TCCACTCCCCTATTATCAAATATAAAAATGGCGTTTCAGTTATTATGCAAGAAACTTGCTATGA AAAATCATTTACTAGTTTCAGATTTTGAAATCGAGGAAATACAAAACACGATATATAATGCTGC CAGAGAATGGAGATGTGAAACATAGGTACCTCCATCCTTGACTGCTGTGACAGTTAAACCAGCA CTGGTGCTTCCAAGACCAGTTGTGATCACTGAACGGTTGCTCAATTTAGCAGCAAATTTCAGAA ACTGGGATTTGCCAGTGCCTAAAATTTAATAGAACACAATTCAGTTAGAGATAATATATATTTC ATATAGTCTGTTTGTAGTGTCTCTATTAGGAACACCAAATGCACTTTATGAAGCTTGTGGATGT CAAGCAGTAGTTCTGTACTTTTATGAATCCAAAAGAAGTTATTTGTGCGTCCCTGGGAAGGAAG ATGCAAGTGGGGCTGGTCAAGCTGACCTACTCTACTTCTTTTTTTTTTCAAATATAGATGCATA AATCTGACTACAAGTTAGTTCTAGTCTAAATGAATATGCCTAACCTTTTCTTTAAGTCTGTCAC CAGTCCAGTGGATTGAGCAAACATTCTAGAACACACTGAATAGTAGCTTGGACCTACCTATAGC GATGTTTCGTTCAGTCTGTTCTTGATAGGATAGGGTAGAGGATGCCTTTCTGTGTGGTGCTGTA GGATTTCTTGTAACGGGTCAATTACCAAGGTAAAATGCATTGGGACAGAGGACCCCCTTTCCCA ACCCCTCTTTGATAGTGAAATTGCTCCCTGGAACATCATTGAGCAGCACGTTCGAGGTTGATGA ACCAAAAAGGATGGATATCTATTCTCTCCACTTTGGCCCTAAGCCTAGTTGTTGTATCTATCCC CACTGGACTGAATTGAACATTTCTGCCATATACAAGAGGCAGTGTTGGAATTTGTGCAGTGCAC GAGTAACATTTTGGTATACAGGAAATCATCTTGGATGCACTTTTTCACGCAGGTGCTTCAGCAG GAGAGGATTGAGTCCATGTACGGACAAGCTAGATGATGGCGTCCAGCTATGGGTCAAGCTAGTT TTCCAGGAGCTTAGAGAAGGTCTGGCCGAAGATATGGACTAAGCTAGCCTAGCGGTAATCGGTA GTCCACTGTGTAGCTATTACAGTGAAAGCATATTAACTGAATATGTTGTTTTCTGGAAAAAGGT TAAACACTAGTTCAACTTATGTACAGAAAGGATGGTTTTGATTTAAGTATATAACAAATGTTAT GGATATTAGTACCTGGATCACCGACAAGAAGCATATGTGGTTCTCCACGAACCTTTGTTCCTGA AGCATCAACATGCTGCACCCCGCCAATTAAGGTAAGAGCAACTGAAAATTAAACAGATCATGAA ACAATACCAACTATAGCAGTTGTTATAGATACTCCAAAAAAGAGTTAAATAGCTTTTCAGAAAC AATCTCCAACTGGATTTTCAGAGAAAGCTAAACACAAGTGAATTACTCGCAATGATTTAGAATA CTGGCAAATAGTTCTGTACCTGCAAGCTTCACTGTGAATAACCCATAAATTTGAGGACAAATGC CTTTCAAGATGGAATTTCTTCCTGTGTACCCAAAAGAACAAAAGAATGCACAGTTTACAGTGCA ATAATGCATTAAATAAAAGGTAGAAAGCTTCCTAATATTATATTTGGGAACAGGATCAGGGTCC ATAGTTCAAATGTAAGTCAATGCACAATTTACAGTGCAATAATGCATTAAATAAAAGGTAGAAA GCTTCCTAATACTTCCTCCATTCCAAATTGATCATATTACATGGGTGCATTAAGACCAAGAAAA TATAATAACTATCTCATACTACATTTACTCTGACAACAAACTCGATGCATGCACATTCTCCACT ATTTTTTAGCCAATAGCAAATCAAAACACTTTGTGCATGGATGCATGCATCAATGTTCATTTAC TTCAATGCACAAATAACAAATAAACTTAATGAATGAACACAAATATGATGATCATTTTAGAATA TTCTTAAAAATATTATATGATGATCAATTTGGAATGGAGGGAGTATTACATTTGGGAACAGGAT CAGGGTTTTTGGTCAAATGTAAGTCAATAAGACAATGTAGTACATTGTAATTGCTTCAATATTA GCTCACAGGATTCAGGCCAAGATCTAGACAGCAACAAAAGGAAAATAAGCTGACATTGGAACAT AAGCACCTTTCAGTGGAGTAGCTCTGGAAGCTGCCCAGAATTCCTCAAACTTGTTGATAATTTC AACAGGTATGTCTAGATCAGACTTCAGTTCATTGGTTCTCCTGTTGAGTGCAGTACATAGACAT TCATAATGCACAGAAGGCAAGACTAGCTGATATCTTTGAACTTGAAACCAAGGAACAGAAAAGG CGCTAAAATTAAAATACTAAAGTGCTAAAAATATGCCCAACTATGGTTGCATCCAACTACCTTG GCAGGCAATAAAATGAAAAATAAGAAAAACTACAACGCAATAAACTAGTAGAGTGAAAGTACAG TGTATGGGAAGCCTTTCCTTCATATGAGTAATGAGACCAGACAATACTACTCTGTTGTCATAAA CTGCATGTTTCCTAAATGTCTTTTTTAGTTTTTCATCATTTAACTAATAAACACCAAAAGCCAT TCCATGTACTTTCTGGATCGGCAATTATGTGCCAAAGCCATTGCTGGTATTATTACTGTGATAG ATTTGGAGAAGCTTAAATGAGCCCCTGCAGGTTCGTTAAAACAACACTATGTAATGAAATACAC TAAGAATGGCACATTATTGCTGGTATTACTGTGATAGATCTTCAGAAGTATAATGTGTCCCTTC AGGTTCGTAAGAAGAACACTATGCAATAAAATATGCTAAGAAGAGAAGAAACTATACCTGGTTC ATTGCATACTCTAAATATGTTCAAAGAATCATAATTTGGAAGATTAAAATTAGAGAACTAATAT TTGGAGAACACCCTCAGTATAAGTTATCAGTTACCTCACAAAATTGGCAATTAGCATAGGATCA AGGTTTGAACGTACATCCTTGATGTCAGGAGACCATTTTGCAGATAATCGACCCGTAACAACAA CATCAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.10161074

Conservation Information
Conserved circRNAs	NA
PMCS	0.10161074

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							6093655-6092016(+)
6092008-6095198(-)
6095124-6095198(-)
						
						Potential amino acid sequence
						
							MPFKMEFLPFSGVALEAAQNSSNLLIISTGMSRSDFSSLVLLTKLAISIGSRFERTSLMSGDHF
ADNRPVTTTSSIKFIYAA*(+)
MNLIDDVVVTGRLSAKWSPDIKDVRSNLDPMLIANFVRRTNELKSDLDIPVEIINKFEEFWAAS
RATPLKGRNSILKGICPQIYGLFTVKLAVALTLIGGVQHVDASGTKVRGEPHMLLVGDPGTGKS
QFLKFAAKLSNRSVITTGLGSTSAGLTVTAVKDGGEWMLEAGALVLADGGLCCIDEFDR*(-)
MLIANFVRRTNELKSDLDIPVEIINKFEEFWAASRATPLKGRNSILKGICPQIYGLFTVKLAVA
LTLIGGVQHVDASGTKVRGEPHMLLVGDPGTGKSQFLKFAAKLSNRSVITTGLGSTSAGLTVTA
VKDGGEWMLEAGALVLADGGLCCIDEFDR*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	6093655-6092016(+) 6092008-6095198(-) 6095124-6095198(-)
Potential amino acid sequence	MPFKMEFLPFSGVALEAAQNSSNLLIISTGMSRSDFSSLVLLTKLAISIGSRFERTSLMSGDHF ADNRPVTTTSSIKFIYAA(+) MNLIDDVVVTGRLSAKWSPDIKDVRSNLDPMLIANFVRRTNELKSDLDIPVEIINKFEEFWAAS RATPLKGRNSILKGICPQIYGLFTVKLAVALTLIGGVQHVDASGTKVRGEPHMLLVGDPGTGKS QFLKFAAKLSNRSVITTGLGSTSAGLTVTAVKDGGEWMLEAGALVLADGGLCCIDEFDR(-) MLIANFVRRTNELKSDLDIPVEIINKFEEFWAASRATPLKGRNSILKGICPQIYGLFTVKLAVA LTLIGGVQHVDASGTKVRGEPHMLLVGDPGTGKSQFLKFAAKLSNRSVITTGLGSTSAGLTVTA VKDGGEWMLEAGALVLADGGLCCIDEFDR*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os06g0218500_circ_g.5

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