Plant Circular RNA Database

Detail information of AT2G35630_circ_g.24

			
						 General Information
					
						CircRNA Name
						AT2G35630_circ_g.24
					
						ID in PlantcircBase
						ath_circ_016634
					
						Alias
						At_ciR5286
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr2: 14971888-14972630  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						PcircRNA_finder, find_circ, CIRI-full
					
						Parent gene
						
							AT2G35630						
					
						Parent gene annotation
						
							Protein MOR1
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT2G35630_circ_g.1 AT2G35630_circ_g.2 AT2G35630_circ_g.3 AT2G35630_circ_g.4 AT2G35630_circ_g.5 AT2G35630_circ_g.6 AT2G35630_circ_g.7 AT2G35630_circ_g.8 AT2G35630_circ_g.9 AT2G35630_circ_g.10 AT2G35630_circ_g.11 AT2G35630_circ_g.12 AT2G35630_circ_g.13 AT2G35630_circ_g.14 AT2G35630_circ_g.15 AT2G35630_circ_g.16 AT2G35630_circ_g.17 AT2G35630_circ_g.18 AT2G35630_circ_g.19 AT2G35630_circ_g.20 AT2G35630_circ_g.21 AT2G35630_circ_g.22 AT2G35630_circ_g.23 AT2G35630_circ_g.25 AT2G35630_circ_g.26 AT2G35630_circ_g.27 AT2G35630_circ_g.28 AT2G35630_circ_g.29 AT2G35630_circ_g.30 AT2G35630_circ_g.31 AT2G35630_circ_g.32 AT2G35630_circ_g.33 AT2G35630_circ_g.34 AT2G35630_circ_g.35 						
					
						Support reads
						2/1
					
						Tissues
						leaf/whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT2G35630.1:3
AT2G35630.2:3

General Information
CircRNA Name	AT2G35630_circ_g.24
ID in PlantcircBase	ath_circ_016634
Alias	At_ciR5286
Organism	Arabidpsis thaliana
Position	chr2: 14971888-14972630 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	PcircRNA_finder, find_circ, CIRI-full
Parent gene	AT2G35630
Parent gene annotation	Protein MOR1
Parent gene strand	+
Alternative splicing	AT2G35630_circ_g.1 AT2G35630_circ_g.2 AT2G35630_circ_g.3 AT2G35630_circ_g.4 AT2G35630_circ_g.5 AT2G35630_circ_g.6 AT2G35630_circ_g.7 AT2G35630_circ_g.8 AT2G35630_circ_g.9 AT2G35630_circ_g.10 AT2G35630_circ_g.11 AT2G35630_circ_g.12 AT2G35630_circ_g.13 AT2G35630_circ_g.14 AT2G35630_circ_g.15 AT2G35630_circ_g.16 AT2G35630_circ_g.17 AT2G35630_circ_g.18 AT2G35630_circ_g.19 AT2G35630_circ_g.20 AT2G35630_circ_g.21 AT2G35630_circ_g.22 AT2G35630_circ_g.23 AT2G35630_circ_g.25 AT2G35630_circ_g.26 AT2G35630_circ_g.27 AT2G35630_circ_g.28 AT2G35630_circ_g.29 AT2G35630_circ_g.30 AT2G35630_circ_g.31 AT2G35630_circ_g.32 AT2G35630_circ_g.33 AT2G35630_circ_g.34 AT2G35630_circ_g.35
Support reads	2/1
Tissues	leaf/whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT2G35630.1:3 AT2G35630.2:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GAAAGAAGAGATAGAAGGTCTACAAGAACTAGACAAGTCGGTGGAAATCTTGGTCCGGTTGCTG
TGTGCTGTTCCTGGTTGGAATGAGAAAAATGTGCAGcaggcgagcaagaaggttgggactggca
aaccgggtggaggcaaaaaagatggatctgtaaggaatgaaggttcaaaatctgttgaaccacc
tgaagatg

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 324
Transcript exons: 14971888-14971992,14972088-14972193,14972518-14972630
CAGGCGAGCAAGAAGGTTGGGACTGGCAAACCGGGTGGAGGCAAAAAAGATGGATCTGTAAGGA
ATGAAGGTTCAAAATCTGTTGAACCACCTGAAGATGTCGAGCCTGCCGAGATGGGTCTTGAAGA
GATTGAAAACAGATTAGGTTCTCTTGTGAAACCAGAAACTGTATCTCAGTTAAAGAGCTCTGTT
TGGAAAGAAAGACTTGAAGCAACTTTGGCTTTGAAAGAAGAGATAGAAGGTCTACAAGAACTAG
ACAAGTCGGTGGAAATCTTGGTCCGGTTGCTGTGTGCTGTTCCTGGTTGGAATGAGAAAAATGT
GCAG
						
						Genomic sequence
						
							CAGGCGAGCAAGAAGGTTGGGACTGGCAAACCGGGTGGAGGCAAAAAAGATGGATCTGTAAGGA
ATGAAGGTTCAAAATCTGTTGAACCACCTGAAGATGTCGAGGTACATAGAAATATAATTATAAA
TGGTTATGTGATTTACTATGTCCTTCTTCAGACTTTGATGGTATTAATATCTTATTCTTCTTTG
CATTTTAGCCTGCCGAGATGGGTCTTGAAGAGATTGAAAACAGATTAGGTTCTCTTGTGAAACC
AGAAACTGTATCTCAGTTAAAGAGCTCTGTTTGGAAAGAAAGACTTGAAGGTTTGTTAAAGGCC
TTGAGGACTCTTTTATTCTCAAATACTGTTATCTTCATCATTAGTTCTTACTTCCTTCTTTGTT
CATTTCTTTTTGCGTTTTGCTTGGGTGGTTAATCTCATTTTAAGTGGTTCATCATCAAATTTTA
CCACACATAGGATAAATCTGTTGCGCAAGGTTCCTGAATTGATATTAAAGCTCTATTTCATGTT
GCCTCTTATTGCTTCAGATCAAAGCTGCATTAGCACATGTCACAGAATCTCTTTGCTAGCTCTT
TAAAAAAAAAGTATTTATCAGTTTGGTGATTTTTTGCATTATATTGCCGTGCAGCAACTTTGGC
TTTGAAAGAAGAGATAGAAGGTCTACAAGAACTAGACAAGTCGGTGGAAATCTTGGTCCGGTTG
CTGTGTGCTGTTCCTGGTTGGAATGAGAAAAATGTGCAG

Sequences
Splice junction sequence	GAAAGAAGAGATAGAAGGTCTACAAGAACTAGACAAGTCGGTGGAAATCTTGGTCCGGTTGCTG TGTGCTGTTCCTGGTTGGAATGAGAAAAATGTGCAGcaggcgagcaagaaggttgggactggca aaccgggtggaggcaaaaaagatggatctgtaaggaatgaaggttcaaaatctgttgaaccacc tgaagatg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 324 Transcript exons: 14971888-14971992,14972088-14972193,14972518-14972630 CAGGCGAGCAAGAAGGTTGGGACTGGCAAACCGGGTGGAGGCAAAAAAGATGGATCTGTAAGGA ATGAAGGTTCAAAATCTGTTGAACCACCTGAAGATGTCGAGCCTGCCGAGATGGGTCTTGAAGA GATTGAAAACAGATTAGGTTCTCTTGTGAAACCAGAAACTGTATCTCAGTTAAAGAGCTCTGTT TGGAAAGAAAGACTTGAAGCAACTTTGGCTTTGAAAGAAGAGATAGAAGGTCTACAAGAACTAG ACAAGTCGGTGGAAATCTTGGTCCGGTTGCTGTGTGCTGTTCCTGGTTGGAATGAGAAAAATGT GCAG
Genomic sequence	CAGGCGAGCAAGAAGGTTGGGACTGGCAAACCGGGTGGAGGCAAAAAAGATGGATCTGTAAGGA ATGAAGGTTCAAAATCTGTTGAACCACCTGAAGATGTCGAGGTACATAGAAATATAATTATAAA TGGTTATGTGATTTACTATGTCCTTCTTCAGACTTTGATGGTATTAATATCTTATTCTTCTTTG CATTTTAGCCTGCCGAGATGGGTCTTGAAGAGATTGAAAACAGATTAGGTTCTCTTGTGAAACC AGAAACTGTATCTCAGTTAAAGAGCTCTGTTTGGAAAGAAAGACTTGAAGGTTTGTTAAAGGCC TTGAGGACTCTTTTATTCTCAAATACTGTTATCTTCATCATTAGTTCTTACTTCCTTCTTTGTT CATTTCTTTTTGCGTTTTGCTTGGGTGGTTAATCTCATTTTAAGTGGTTCATCATCAAATTTTA CCACACATAGGATAAATCTGTTGCGCAAGGTTCCTGAATTGATATTAAAGCTCTATTTCATGTT GCCTCTTATTGCTTCAGATCAAAGCTGCATTAGCACATGTCACAGAATCTCTTTGCTAGCTCTT TAAAAAAAAAGTATTTATCAGTTTGGTGATTTTTTGCATTATATTGCCGTGCAGCAACTTTGGC TTTGAAAGAAGAGATAGAAGGTCTACAAGAACTAGACAAGTCGGTGGAAATCTTGGTCCGGTTG CTGTGTGCTGTTCCTGGTTGGAATGAGAAAAATGTGCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.267318646

Conservation Information
Conserved circRNAs	NA
PMCS	0.267318646

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							14972097-14972627(+)
						
						Potential amino acid sequence
						
							MGLEEIENRLGSLVKPETVSQLKSSVWKERLEATLALKEEIEGLQELDKSVEILVRLLCAVPGW
NEKNVQQASKKVGTGKPGGGKKDGSVRNEGSKSVEPPEDVEPAEMGLEEIENRLGSLVKPETVS
QLKSSVWKERLEATLALKEEIEGLQELDKSVEILVRLLCAVPGWNEKNVQQASKKVGTGKPGGG
KKDGSVRNEGSKSVEPPEDVEPAEMGLEEIENRLGSLVKPETVSQLKSSVWKERLEATLALKEE
IEGLQELDKSVEILVRLLCAVPGWNEKNVQQASKKVGTGKPGGGKKDGSVRNEGSKSVEPPEDV
EPAEMGLEEIENRLGSLVKPETVSQLKSSVWKERLEATLALKEEIEGLQELDKSVEILVRLLCA
VPGWNEKNVQ(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	14972097-14972627(+)
Potential amino acid sequence	MGLEEIENRLGSLVKPETVSQLKSSVWKERLEATLALKEEIEGLQELDKSVEILVRLLCAVPGW NEKNVQQASKKVGTGKPGGGKKDGSVRNEGSKSVEPPEDVEPAEMGLEEIENRLGSLVKPETVS QLKSSVWKERLEATLALKEEIEGLQELDKSVEILVRLLCAVPGWNEKNVQQASKKVGTGKPGGG KKDGSVRNEGSKSVEPPEDVEPAEMGLEEIENRLGSLVKPETVSQLKSSVWKERLEATLALKEE IEGLQELDKSVEILVRLLCAVPGWNEKNVQQASKKVGTGKPGGGKKDGSVRNEGSKSVEPPEDV EPAEMGLEEIENRLGSLVKPETVSQLKSSVWKERLEATLALKEEIEGLQELDKSVEILVRLLCA VPGWNEKNVQ(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of AT2G35630_circ_g.24

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