Plant Circular RNA Database

Detail information of Os03g0749800_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os03g0749800_circ_g.4
					
						ID in PlantcircBase
						osa_circ_021918
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 30890835-30892999  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder
					
						Parent gene
						
							Os03g0749800						
					
						Parent gene annotation
						
							Similar to Tousled-like protein kinase. (Os03t0749800-01);Simila
r to Tousled-like kinase (Fragment). (Os03t0749800-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os03g0749800_circ_g.1 Os03g0749800_circ_g.2 Os03g0749800_circ_g.3 Os03g0749800_circ_g.5 Os03g0749800_circ_g.6 						
					
						Support reads
						5
					
						Tissues
						shoot, root, seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0749800-02:3

General Information
CircRNA Name	Os03g0749800_circ_g.4
ID in PlantcircBase	osa_circ_021918
Alias	NA
Organism	Oryza sativa
Position	chr3: 30890835-30892999 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder
Parent gene	Os03g0749800
Parent gene annotation	Similar to Tousled-like protein kinase. (Os03t0749800-01);Simila r to Tousled-like kinase (Fragment). (Os03t0749800-02)
Parent gene strand	-
Alternative splicing	Os03g0749800_circ_g.1 Os03g0749800_circ_g.2 Os03g0749800_circ_g.3 Os03g0749800_circ_g.5 Os03g0749800_circ_g.6
Support reads	5
Tissues	shoot, root, seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0749800-02:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TTAAGATAAACTAGGCCTTGAAATATTTGGACAATTATGATTCTCGCTTCCTTTTCTGGAAGAA
TCGGTGTTGCTTTAAGGACTGCATCAAGATCTTTGCctttgcctcatttgatacagctggcttt
gaagggaattccactctgcgtgcgttgatgattgtatcttcccgaagtattctctcctgggtct
ggtcatgg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 482
Transcript exons: 30890835-30890996,30892285-30892339,30892735-30892999
CTTTGCCTCATTTGATACAGCTGGCTTTGAAGGGAATTCCACTCTGCGTGCGTTGATGATTGTA
TCTTCCCGAAGTATTCTCTCCTGGGTCTGGTCATGGCCAAAAGGACGCCTTCCGAAGAGCATTT
GGTAAAACATTACACCCGCTGACCAAACATCCACCTTCGATGAGATAAATGGCGTTTTGCTAAG
ATCAAAGCATTCCGGTGGCAGGTACCAATAGGTTCCAGCTCCCTGAGAAGTCAGTTCCATTCCC
TGAGACCCAACATCATCCTCCACTATCTTGCTGAGGCCAAAGTCTGTAACTTTTGCAACACCAA
CCTCATCGAAGAGAACATTGCCTGGTTTGAGATCATAGTGAATGATCTTTTGAGTTCTCTTGTT
AAGATAAACTAGGCCTTGAAATATTTGGACAATTATGATTCTCGCTTCCTTTTCTGGAAGAATC
GGTGTTGCTTTAAGGACTGCATCAAGATCTTTGC
						
					

						Genomic sequence
						
							CTTTGCCTCATTTGATACAGCTGGCTTTGAAGGGAATTCCACTCTGCGTGCGTTGATGATTGTA
TCTTCCCGAAGTATTCTCTCCTGGGTCTGGTCATGGCCAAAAGGACGCCTTCCGAAGAGCATTT
GGTAAAACATTACACCCGCTGACCAAACATCCACCTGCAGGAATGCAAACATCAATTTTACCAC
CAATCAGAACAAAGATGAAGTTGCCCATAAGTGGTTTAAATTGATTACTCAGGGGGTTCACAAA
TGACTCTAACTGAAATAAGGGTTAAGAAGGAATCGCGTAACATTTTGAGTAAAAATTGCATATC
CACATGGCCATTGCCTGCATACCACTAAGCCCAACCAGTATTCCCCATCCCCATCAACAGCAGA
TCAATTCACCACCTGTGACCTGCACCTTCCTGCTCTTGCACCTCCTTCTGAAAACTAACCATCT
GCACGGCCATTTCCCCTGGATCTGTCCAATGGGAACTACTATCAGACTGATCTATTTGGCACAT
AATTGTCTCGATATTGACACAATTTAAGCTGTGCATGTGAGTTTCTTGATAGTTTTCCCTGATT
TTCCTCATTCGCCACCTCTTTCCTGTTCTGTATTAAGTAACACTCTCATGATGTTGATCTTCTT
CCTATTTTCCATAGTGCAAGAATTGGACTGGACGGTGGTAAAACTAGAAATGATCTGAACCAGT
GAATGGTCTGGTTTGGCTTAGAAGAATGTCGGTCCATGTATACGAACTACGATAAAACCATATC
CAGTCCTACCAGCTCACAGCCTAAATGCAAAAAAATAAAAATAAAAAATGTTATGTATGCTGGG
AATTGAACCCTGCAACTAGGACAGGCCTTGCAAAGTACTCCCACAATGCATCTAAGTACCTAAC
CAACTGGATAATGGTGATTTCCTCGCTTTAATGACAAACGAGTATATTCAGCCTGTTATTTTAC
TTACCAAACTGGCAAAACTATTATTTTCTCAAATCAGCATTTGGACCGGTGAAGTGCAGTCCAA
CTGGCAAAAAGCAGAACCAAGGAGCTCACTAACTCAATCAATGGTCCAGGTTTTTGTACTACGA
AGTATATTCTTTCTGAGGTTTTCAAGTATGGATAGAAAGCTCATCTTCTCTACTTATCTCCACG
TAGTGTCATAGCGTTCTCAATGCCCGTCAAAATACATCTTATAATTTGTACCAGAGGGAGTAAT
GCTAATATCATATTAACATACTAGGGATTACTACTTAATGAATGCTGAAAATTTGGCAAGTGAG
CATGATTTGCGCATGACTGCGGTCTCTGTTTATGACAAACAAACGCCACAAGAATCCCTAACAT
CCTTATAGAAGGATCTTATTGATGCATGTTTTTGTCAAATTGCTTTAACACACTAAGATATGAA
AGATTTATTAACATTAAAAGGAAAACATGTTGCCAAATATACCTTCGATGAGATAAATGGCGTT
TTGCTAAGATCAAAGCATTCCGGTGGCAGGTACCTGGAAGAAAAAGAAGAACATTAGGAAACAA
ATGATTGACATGCCAAAAGAACAATGGTTCTAAATGGGAAACAGCAACACTAACATAAATAAAA
ACTAAATTTATGACATCATTTGTTCTATCCCACCTGTTTACATAAGACACTAGAGAAAAAAAAT
ATTTCATTGGTAGATAGTAGCATGAAAATGGTTGTACTTCATGCAATAACTATTAATTCCCTTA
GGTTCTAGAATGATGCATCAAATGTGTGCAACTCTACTACACAGTGCAGGCATTGTTGCACCAT
GTATACCAATTTAATGCCAGCAGATAAAAGATTCCATAAGAGGTAGTCAGATAGAGTATTTTAT
CAATTTAGTACACAGAAATCAACCGGAAAAGTGAGCACGCTCACCAATAGGTTCCAGCTCCCTG
AGAAGTCAGTTCCATTCCCTGAGACCCAACATCATCCTCCACTATCTTGCTGAGGCCAAAGTCT
GTAACTTTTGCAACACCAACCTCATCGAAGAGAACATTGCCTGGTTTGAGATCATAGTGAATGA
TCTTTTGAGTTCTCTTGTTAAGATAAACTAGGCCTTGAAATATTTGGACAATTATGATTCTCGC
TTCCTTTTCTGGAAGAATCGGTGTTGCTTTAAGGACTGCATCAAGATCTTTGC
						
					

			
		

Sequences
Splice junction sequence	TTAAGATAAACTAGGCCTTGAAATATTTGGACAATTATGATTCTCGCTTCCTTTTCTGGAAGAA TCGGTGTTGCTTTAAGGACTGCATCAAGATCTTTGCctttgcctcatttgatacagctggcttt gaagggaattccactctgcgtgcgttgatgattgtatcttcccgaagtattctctcctgggtct ggtcatgg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 482 Transcript exons: 30890835-30890996,30892285-30892339,30892735-30892999 CTTTGCCTCATTTGATACAGCTGGCTTTGAAGGGAATTCCACTCTGCGTGCGTTGATGATTGTA TCTTCCCGAAGTATTCTCTCCTGGGTCTGGTCATGGCCAAAAGGACGCCTTCCGAAGAGCATTT GGTAAAACATTACACCCGCTGACCAAACATCCACCTTCGATGAGATAAATGGCGTTTTGCTAAG ATCAAAGCATTCCGGTGGCAGGTACCAATAGGTTCCAGCTCCCTGAGAAGTCAGTTCCATTCCC TGAGACCCAACATCATCCTCCACTATCTTGCTGAGGCCAAAGTCTGTAACTTTTGCAACACCAA CCTCATCGAAGAGAACATTGCCTGGTTTGAGATCATAGTGAATGATCTTTTGAGTTCTCTTGTT AAGATAAACTAGGCCTTGAAATATTTGGACAATTATGATTCTCGCTTCCTTTTCTGGAAGAATC GGTGTTGCTTTAAGGACTGCATCAAGATCTTTGC
Genomic sequence	CTTTGCCTCATTTGATACAGCTGGCTTTGAAGGGAATTCCACTCTGCGTGCGTTGATGATTGTA TCTTCCCGAAGTATTCTCTCCTGGGTCTGGTCATGGCCAAAAGGACGCCTTCCGAAGAGCATTT GGTAAAACATTACACCCGCTGACCAAACATCCACCTGCAGGAATGCAAACATCAATTTTACCAC CAATCAGAACAAAGATGAAGTTGCCCATAAGTGGTTTAAATTGATTACTCAGGGGGTTCACAAA TGACTCTAACTGAAATAAGGGTTAAGAAGGAATCGCGTAACATTTTGAGTAAAAATTGCATATC CACATGGCCATTGCCTGCATACCACTAAGCCCAACCAGTATTCCCCATCCCCATCAACAGCAGA TCAATTCACCACCTGTGACCTGCACCTTCCTGCTCTTGCACCTCCTTCTGAAAACTAACCATCT GCACGGCCATTTCCCCTGGATCTGTCCAATGGGAACTACTATCAGACTGATCTATTTGGCACAT AATTGTCTCGATATTGACACAATTTAAGCTGTGCATGTGAGTTTCTTGATAGTTTTCCCTGATT TTCCTCATTCGCCACCTCTTTCCTGTTCTGTATTAAGTAACACTCTCATGATGTTGATCTTCTT CCTATTTTCCATAGTGCAAGAATTGGACTGGACGGTGGTAAAACTAGAAATGATCTGAACCAGT GAATGGTCTGGTTTGGCTTAGAAGAATGTCGGTCCATGTATACGAACTACGATAAAACCATATC CAGTCCTACCAGCTCACAGCCTAAATGCAAAAAAATAAAAATAAAAAATGTTATGTATGCTGGG AATTGAACCCTGCAACTAGGACAGGCCTTGCAAAGTACTCCCACAATGCATCTAAGTACCTAAC CAACTGGATAATGGTGATTTCCTCGCTTTAATGACAAACGAGTATATTCAGCCTGTTATTTTAC TTACCAAACTGGCAAAACTATTATTTTCTCAAATCAGCATTTGGACCGGTGAAGTGCAGTCCAA CTGGCAAAAAGCAGAACCAAGGAGCTCACTAACTCAATCAATGGTCCAGGTTTTTGTACTACGA AGTATATTCTTTCTGAGGTTTTCAAGTATGGATAGAAAGCTCATCTTCTCTACTTATCTCCACG TAGTGTCATAGCGTTCTCAATGCCCGTCAAAATACATCTTATAATTTGTACCAGAGGGAGTAAT GCTAATATCATATTAACATACTAGGGATTACTACTTAATGAATGCTGAAAATTTGGCAAGTGAG CATGATTTGCGCATGACTGCGGTCTCTGTTTATGACAAACAAACGCCACAAGAATCCCTAACAT CCTTATAGAAGGATCTTATTGATGCATGTTTTTGTCAAATTGCTTTAACACACTAAGATATGAA AGATTTATTAACATTAAAAGGAAAACATGTTGCCAAATATACCTTCGATGAGATAAATGGCGTT TTGCTAAGATCAAAGCATTCCGGTGGCAGGTACCTGGAAGAAAAAGAAGAACATTAGGAAACAA ATGATTGACATGCCAAAAGAACAATGGTTCTAAATGGGAAACAGCAACACTAACATAAATAAAA ACTAAATTTATGACATCATTTGTTCTATCCCACCTGTTTACATAAGACACTAGAGAAAAAAAAT ATTTCATTGGTAGATAGTAGCATGAAAATGGTTGTACTTCATGCAATAACTATTAATTCCCTTA GGTTCTAGAATGATGCATCAAATGTGTGCAACTCTACTACACAGTGCAGGCATTGTTGCACCAT GTATACCAATTTAATGCCAGCAGATAAAAGATTCCATAAGAGGTAGTCAGATAGAGTATTTTAT CAATTTAGTACACAGAAATCAACCGGAAAAGTGAGCACGCTCACCAATAGGTTCCAGCTCCCTG AGAAGTCAGTTCCATTCCCTGAGACCCAACATCATCCTCCACTATCTTGCTGAGGCCAAAGTCT GTAACTTTTGCAACACCAACCTCATCGAAGAGAACATTGCCTGGTTTGAGATCATAGTGAATGA TCTTTTGAGTTCTCTTGTTAAGATAAACTAGGCCTTGAAATATTTGGACAATTATGATTCTCGC TTCCTTTTCTGGAAGAATCGGTGTTGCTTTAAGGACTGCATCAAGATCTTTGC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_005108* osi_circ_013654 						
					
						PMCS
						0.24445776

Conservation Information
Conserved circRNAs	osi_circ_005108* osi_circ_013654
PMCS	0.24445776

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							30892937-30890887(+)
30890934-30892989(-)
30892771-30892936(-)
						
						Potential amino acid sequence
						
							MILASFSGRIGVALRTASRSLPLPHLIQLALKGIPLCVR*(+)
MTRPRREYFGKIQSSTHAEWNSLQSQLYQMRQRQRS*(-)
MELTSQGAGTYWYLPPECFDLSKTPFISSKVDVWSAGVMFYQMLFGRRPFGHDQTQERILREDT
IINARRVEFPSKPAVSNEAKAKILMQSLKQHRFFQKRKRES*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	30892937-30890887(+) 30890934-30892989(-) 30892771-30892936(-)
Potential amino acid sequence	MILASFSGRIGVALRTASRSLPLPHLIQLALKGIPLCVR(+) MTRPRREYFGKIQSSTHAEWNSLQSQLYQMRQRQRS(-) MELTSQGAGTYWYLPPECFDLSKTPFISSKVDVWSAGVMFYQMLFGRRPFGHDQTQERILREDT IINARRVEFPSKPAVSNEAKAKILMQSLKQHRFFQKRKRES*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os03g0749800_circ_g.4

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