Plant Circular RNA Database

Detail information of AT5G03650_circ_g.15

			
						 General Information
					
						CircRNA Name
						AT5G03650_circ_g.15
					
						ID in PlantcircBase
						ath_circ_036212
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 935687-936321  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, MapSplice, PcircRNA_finder
					
						Parent gene
						
							AT5G03650						
					
						Parent gene annotation
						
							1,4-alpha-glucan-branching enzyme 2-2, chloroplastic/amyloplasti
c
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT5G03650_circ_g.11 AT5G03650_circ_g.12 AT5G03650_circ_g.13 AT5G03650_circ_g.14 AT5G03650_circ_g.16 AT5G03650_circ_g.17 AT5G03650_circ_g.18 AT5G03650_circ_g.19 AT5G03650_circ_g.20 AT5G03650_circ_g.21 AT5G03650_circ_g.22 AT5G03650_circ_g.23 						
					
						Support reads
						8/2/NA
					
						Tissues
						inflorescences/seedlings/leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G03650.1:3

General Information
CircRNA Name	AT5G03650_circ_g.15
ID in PlantcircBase	ath_circ_036212
Alias	NA
Organism	Arabidpsis thaliana
Position	chr5: 935687-936321 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, MapSplice, PcircRNA_finder
Parent gene	AT5G03650
Parent gene annotation	1,4-alpha-glucan-branching enzyme 2-2, chloroplastic/amyloplasti c
Parent gene strand	+
Alternative splicing	AT5G03650_circ_g.11 AT5G03650_circ_g.12 AT5G03650_circ_g.13 AT5G03650_circ_g.14 AT5G03650_circ_g.16 AT5G03650_circ_g.17 AT5G03650_circ_g.18 AT5G03650_circ_g.19 AT5G03650_circ_g.20 AT5G03650_circ_g.21 AT5G03650_circ_g.22 AT5G03650_circ_g.23
Support reads	8/2/NA
Tissues	inflorescences/seedlings/leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G03650.1:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TACCAACAGAAGGTGGTCAGAGAAGTGTATCTCTTATGCTGAAAGTCACGATCAAGCTCTTGTT
GGTGATAAAACAATTGCCTTCTGGTTAATGGACAAGgttggatttactgggaactacaccgaat
actttggattggaaactgatgtggatgctgtgaattatctcatgctggttaatgatatgattca
tgggctct

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 378
Transcript exons: 935687-935815,935982-936085,936177-936321
GTTGGATTTACTGGGAACTACACCGAATACTTTGGATTGGAAACTGATGTGGATGCTGTGAATT
ATCTCATGCTGGTTAATGATATGATTCATGGGCTCTACCCTGAAGCGATTACCGTTGGTGAAGA
TGTTAGTGGTATGCCAACATTCTGTATTCCTGTCCAAGATGGTGGCGTTGGATTTGACTACCGT
TTACACATGGCCATAGCTGATAAGTGGATAGAAATGCTCAAGAAGAGAGATGAAGACTGGCAAA
TGGGCGACATCATTTACACACTTACCAACAGAAGGTGGTCAGAGAAGTGTATCTCTTATGCTGA
AAGTCACGATCAAGCTCTTGTTGGTGATAAAACAATTGCCTTCTGGTTAATGGACAAG
						
						Genomic sequence
						
							GTTGGATTTACTGGGAACTACACCGAATACTTTGGATTGGAAACTGATGTGGATGCTGTGAATT
ATCTCATGCTGGTTAATGATATGATTCATGGGCTCTACCCTGAAGCGATTACCGTTGGTGAAGA
TGTAAGTTTTCTGATTGGCTAAATAAAAAAGTGCTCTACAGAACCATAAGGGATACATATTGTG
TATCATATTAAAGATAGTCCAAAACCAAATTAGTGTGAACTGACAAGAGAAAGTTAGTATATAT
TTTTTGGTGATCTATGCTAATAAAATCGTTTCATTTCAGGTTAGTGGTATGCCAACATTCTGTA
TTCCTGTCCAAGATGGTGGCGTTGGATTTGACTACCGTTTACACATGGCCATAGCTGATAAGTG
GATAGAAATGCTCAAGTGAGTGTTTTCTGCATTTTCTTTTAAGTTCCATCTGCTTTAATATTTG
GTGAAATAAGTGTCTTACTCTCCTCCATGTTATGCTGTGAAGGAAGAGAGATGAAGACTGGCAA
ATGGGCGACATCATTTACACACTTACCAACAGAAGGTGGTCAGAGAAGTGTATCTCTTATGCTG
AAAGTCACGATCAAGCTCTTGTTGGTGATAAAACAATTGCCTTCTGGTTAATGGACAAG

Sequences
Splice junction sequence	TACCAACAGAAGGTGGTCAGAGAAGTGTATCTCTTATGCTGAAAGTCACGATCAAGCTCTTGTT GGTGATAAAACAATTGCCTTCTGGTTAATGGACAAGgttggatttactgggaactacaccgaat actttggattggaaactgatgtggatgctgtgaattatctcatgctggttaatgatatgattca tgggctct
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 378 Transcript exons: 935687-935815,935982-936085,936177-936321 GTTGGATTTACTGGGAACTACACCGAATACTTTGGATTGGAAACTGATGTGGATGCTGTGAATT ATCTCATGCTGGTTAATGATATGATTCATGGGCTCTACCCTGAAGCGATTACCGTTGGTGAAGA TGTTAGTGGTATGCCAACATTCTGTATTCCTGTCCAAGATGGTGGCGTTGGATTTGACTACCGT TTACACATGGCCATAGCTGATAAGTGGATAGAAATGCTCAAGAAGAGAGATGAAGACTGGCAAA TGGGCGACATCATTTACACACTTACCAACAGAAGGTGGTCAGAGAAGTGTATCTCTTATGCTGA AAGTCACGATCAAGCTCTTGTTGGTGATAAAACAATTGCCTTCTGGTTAATGGACAAG
Genomic sequence	GTTGGATTTACTGGGAACTACACCGAATACTTTGGATTGGAAACTGATGTGGATGCTGTGAATT ATCTCATGCTGGTTAATGATATGATTCATGGGCTCTACCCTGAAGCGATTACCGTTGGTGAAGA TGTAAGTTTTCTGATTGGCTAAATAAAAAAGTGCTCTACAGAACCATAAGGGATACATATTGTG TATCATATTAAAGATAGTCCAAAACCAAATTAGTGTGAACTGACAAGAGAAAGTTAGTATATAT TTTTTGGTGATCTATGCTAATAAAATCGTTTCATTTCAGGTTAGTGGTATGCCAACATTCTGTA TTCCTGTCCAAGATGGTGGCGTTGGATTTGACTACCGTTTACACATGGCCATAGCTGATAAGTG GATAGAAATGCTCAAGTGAGTGTTTTCTGCATTTTCTTTTAAGTTCCATCTGCTTTAATATTTG GTGAAATAAGTGTCTTACTCTCCTCCATGTTATGCTGTGAAGGAAGAGAGATGAAGACTGGCAA ATGGGCGACATCATTTACACACTTACCAACAGAAGGTGGTCAGAGAAGTGTATCTCTTATGCTG AAAGTCACGATCAAGCTCTTGTTGGTGATAAAACAATTGCCTTCTGGTTAATGGACAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.406300756

Conservation Information
Conserved circRNAs	NA
PMCS	0.406300756

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							935756-936318(+)
						
						Potential amino acid sequence
						
							MLVNDMIHGLYPEAITVGEDVSGMPTFCIPVQDGGVGFDYRLHMAIADKWIEMLKKRDEDWQMG
DIIYTLTNRRWSEKCISYAESHDQALVGDKTIAFWLMDKVGFTGNYTEYFGLETDVDAVNYLML
VNDMIHGLYPEAITVGEDVSGMPTFCIPVQDGGVGFDYRLHMAIADKWIEMLKKRDEDWQMGDI
IYTLTNRRWSEKCISYAESHDQALVGDKTIAFWLMDKVGFTGNYTEYFGLETDVDAVNYLMLVN
DMIHGLYPEAITVGEDVSGMPTFCIPVQDGGVGFDYRLHMAIADKWIEMLKKRDEDWQMGDIIY
TLTNRRWSEKCISYAESHDQALVGDKTIAFWLMDKVGFTGNYTEYFGLETDVDAVNYLMLVNDM
IHGLYPEAITVGEDVSGMPTFCIPVQDGGVGFDYRLHMAIADKWIEMLKKRDEDWQMGDIIYTL
TNRRWSEKCISYAESHDQALVGDKTIAFWLMDK(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	935756-936318(+)
Potential amino acid sequence	MLVNDMIHGLYPEAITVGEDVSGMPTFCIPVQDGGVGFDYRLHMAIADKWIEMLKKRDEDWQMG DIIYTLTNRRWSEKCISYAESHDQALVGDKTIAFWLMDKVGFTGNYTEYFGLETDVDAVNYLML VNDMIHGLYPEAITVGEDVSGMPTFCIPVQDGGVGFDYRLHMAIADKWIEMLKKRDEDWQMGDI IYTLTNRRWSEKCISYAESHDQALVGDKTIAFWLMDKVGFTGNYTEYFGLETDVDAVNYLMLVN DMIHGLYPEAITVGEDVSGMPTFCIPVQDGGVGFDYRLHMAIADKWIEMLKKRDEDWQMGDIIY TLTNRRWSEKCISYAESHDQALVGDKTIAFWLMDKVGFTGNYTEYFGLETDVDAVNYLMLVNDM IHGLYPEAITVGEDVSGMPTFCIPVQDGGVGFDYRLHMAIADKWIEMLKKRDEDWQMGDIIYTL TNRRWSEKCISYAESHDQALVGDKTIAFWLMDK(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017;Pan et al., 2017;Liu et al., 2017

circRNA

Detail information of AT5G03650_circ_g.15

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