Plant Circular RNA Database

Detail information of Os07g0421300_circ_g.6

			
						 General Information
					
						CircRNA Name
						Os07g0421300_circ_g.6
					
						ID in PlantcircBase
						osa_circ_033548
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr7: 13541380-13542212  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os07g0421300						
					
						Parent gene annotation
						
							Similar to Alpha glucosidase-like protein. (Os07t0421300-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os07g0421300_circ_g.2 Os07g0421300_circ_g.3 Os07g0421300_circ_g.4 Os07g0421300_circ_g.5 						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os07t0421300-01:4

General Information
CircRNA Name	Os07g0421300_circ_g.6
ID in PlantcircBase	osa_circ_033548
Alias	NA
Organism	Oryza sativa
Position	chr7: 13541380-13542212 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os07g0421300
Parent gene annotation	Similar to Alpha glucosidase-like protein. (Os07t0421300-01)
Parent gene strand	+
Alternative splicing	Os07g0421300_circ_g.2 Os07g0421300_circ_g.3 Os07g0421300_circ_g.4 Os07g0421300_circ_g.5
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os07t0421300-01:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TGGTTTTATAGGACAACAACGCTATGCTGCTATGTGGACTGGTGATAATGTATCAAACTGGGAA
CATCTCCACATGAGTATAGCCATGGTTCTTCAGTTGcatcgtttccctgatccgaaatgtatgg
ttgatgatctccattcggttggctgcaaagcaatatggatgcttgacccaggaatcaagaatga
gagcggtt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 323
Transcript exons: 13541380-13541516,13542027-13542212
CATCGTTTCCCTGATCCGAAATGTATGGTTGATGATCTCCATTCGGTTGGCTGCAAAGCAATAT
GGATGCTTGACCCAGGAATCAAGAATGAGAGCGGTTACTTTGTGTTTGATAGCGGTTTAGAAAG
TGATGTTTGGTCTATGGAATGCTCATGGCAAAATCTACTTATGAGGGAATGAAATTGGCCAATC
CAACTAAACGGCCATTTGTTCTTACTCGAGCTGGTTTTATAGGACAACAACGCTATGCTGCTAT
GTGGACTGGTGATAATGTATCAAACTGGGAACATCTCCACATGAGTATAGCCATGGTTCTTCAG
TTG
Transcript length: 585
Transcript exons: 13541380-13541548,13541635-13541777,13541865-13541951,13
542027-13542212
CATCGTTTCCCTGATCCGAAATGTATGGTTGATGATCTCCATTCGGTTGGCTGCAAAGCAATAT
GGATGCTTGACCCAGGAATCAAGAATGAGAGCGGTTACTTTGTGTTTGATAGCGGTTTAGAAAG
TGATGTTTGGGTTCAAAAGGAAGACAAGCAGCCGTTCGTTGGAGAAGTATGGCCTGGTGATTGT
GTTTTCCCTGATTTTACTTGTGAAAGAGCCCGCTCCTGGTGGTCTGGCTTAGTAAGGCAGTTCG
TCTCTAATAGCGTTGATGGATTATGGAATGATATGAATGAACCTGCTGTTTTTAATACTGCCAC
CAAAACAATGCCAGAAAGCAATATCCATAGAGGAGATGCTAATATTGGTGGTCTCCAGAATCAT
CCGTACTACCATAACGTCTATGGAATGCTCATGGCAAAATCTACTTATGAGGGAATGAAATTGG
CCAATCCAACTAAACGGCCATTTGTTCTTACTCGAGCTGGTTTTATAGGACAACAACGCTATGC
TGCTATGTGGACTGGTGATAATGTATCAAACTGGGAACATCTCCACATGAGTATAGCCATGGTT
CTTCAGTTG
						
					

						Genomic sequence
						
							CATCGTTTCCCTGATCCGAAATGTATGGTTGATGATCTCCATTCGGTTGGCTGCAAAGCAATAT
GGATGCTTGACCCAGGAATCAAGAATGAGAGCGGTTACTTTGTGTTTGATAGCGGTTTAGAAAG
TGATGTTTGGGTTCAAAAGGAAGACAAGCAGCCGTTCGTTGGTATATTTCCTCCAATGAATGAA
CTCCTTCATTACCGTGATGAACATATCACTAATTGATGGGTCTCTTTTACTCATATTGTGTAGG
AGAAGTATGGCCTGGTGATTGTGTTTTCCCTGATTTTACTTGTGAAAGAGCCCGCTCCTGGTGG
TCTGGCTTAGTAAGGCAGTTCGTCTCTAATAGCGTTGATGGATTATGGAATGATATGAATGAAC
CTGCTGTTTTTAATGTACATGATCTGCCATAAATCATTTGTTTTATTTCAACTGGTTTCATCTT
GCTCTTCTCTTACATTGGAAGTGTTTGTCACATCCAGACTGCCACCAAAACAATGCCAGAAAGC
AATATCCATAGAGGAGATGCTAATATTGGTGGTCTCCAGAATCATCCGTACTACCATAACGTAA
CATTTTAACCCTTGGTAAGCTCACCTACAAAATCTAGTCTGACTTCTAACCTTTTGTTGTATAA
CTGTCAGGTCTATGGAATGCTCATGGCAAAATCTACTTATGAGGGAATGAAATTGGCCAATCCA
ACTAAACGGCCATTTGTTCTTACTCGAGCTGGTTTTATAGGACAACAACGCTATGCTGCTATGT
GGACTGGTGATAATGTATCAAACTGGGAACATCTCCACATGAGTATAGCCATGGTTCTTCAGTT
G
						
					

			
		

Sequences
Splice junction sequence	TGGTTTTATAGGACAACAACGCTATGCTGCTATGTGGACTGGTGATAATGTATCAAACTGGGAA CATCTCCACATGAGTATAGCCATGGTTCTTCAGTTGcatcgtttccctgatccgaaatgtatgg ttgatgatctccattcggttggctgcaaagcaatatggatgcttgacccaggaatcaagaatga gagcggtt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 323 Transcript exons: 13541380-13541516,13542027-13542212 CATCGTTTCCCTGATCCGAAATGTATGGTTGATGATCTCCATTCGGTTGGCTGCAAAGCAATAT GGATGCTTGACCCAGGAATCAAGAATGAGAGCGGTTACTTTGTGTTTGATAGCGGTTTAGAAAG TGATGTTTGGTCTATGGAATGCTCATGGCAAAATCTACTTATGAGGGAATGAAATTGGCCAATC CAACTAAACGGCCATTTGTTCTTACTCGAGCTGGTTTTATAGGACAACAACGCTATGCTGCTAT GTGGACTGGTGATAATGTATCAAACTGGGAACATCTCCACATGAGTATAGCCATGGTTCTTCAG TTG Transcript length: 585 Transcript exons: 13541380-13541548,13541635-13541777,13541865-13541951,13 542027-13542212 CATCGTTTCCCTGATCCGAAATGTATGGTTGATGATCTCCATTCGGTTGGCTGCAAAGCAATAT GGATGCTTGACCCAGGAATCAAGAATGAGAGCGGTTACTTTGTGTTTGATAGCGGTTTAGAAAG TGATGTTTGGGTTCAAAAGGAAGACAAGCAGCCGTTCGTTGGAGAAGTATGGCCTGGTGATTGT GTTTTCCCTGATTTTACTTGTGAAAGAGCCCGCTCCTGGTGGTCTGGCTTAGTAAGGCAGTTCG TCTCTAATAGCGTTGATGGATTATGGAATGATATGAATGAACCTGCTGTTTTTAATACTGCCAC CAAAACAATGCCAGAAAGCAATATCCATAGAGGAGATGCTAATATTGGTGGTCTCCAGAATCAT CCGTACTACCATAACGTCTATGGAATGCTCATGGCAAAATCTACTTATGAGGGAATGAAATTGG CCAATCCAACTAAACGGCCATTTGTTCTTACTCGAGCTGGTTTTATAGGACAACAACGCTATGC TGCTATGTGGACTGGTGATAATGTATCAAACTGGGAACATCTCCACATGAGTATAGCCATGGTT CTTCAGTTG
Genomic sequence	CATCGTTTCCCTGATCCGAAATGTATGGTTGATGATCTCCATTCGGTTGGCTGCAAAGCAATAT GGATGCTTGACCCAGGAATCAAGAATGAGAGCGGTTACTTTGTGTTTGATAGCGGTTTAGAAAG TGATGTTTGGGTTCAAAAGGAAGACAAGCAGCCGTTCGTTGGTATATTTCCTCCAATGAATGAA CTCCTTCATTACCGTGATGAACATATCACTAATTGATGGGTCTCTTTTACTCATATTGTGTAGG AGAAGTATGGCCTGGTGATTGTGTTTTCCCTGATTTTACTTGTGAAAGAGCCCGCTCCTGGTGG TCTGGCTTAGTAAGGCAGTTCGTCTCTAATAGCGTTGATGGATTATGGAATGATATGAATGAAC CTGCTGTTTTTAATGTACATGATCTGCCATAAATCATTTGTTTTATTTCAACTGGTTTCATCTT GCTCTTCTCTTACATTGGAAGTGTTTGTCACATCCAGACTGCCACCAAAACAATGCCAGAAAGC AATATCCATAGAGGAGATGCTAATATTGGTGGTCTCCAGAATCATCCGTACTACCATAACGTAA CATTTTAACCCTTGGTAAGCTCACCTACAAAATCTAGTCTGACTTCTAACCTTTTGTTGTATAA CTGTCAGGTCTATGGAATGCTCATGGCAAAATCTACTTATGAGGGAATGAAATTGGCCAATCCA ACTAAACGGCCATTTGTTCTTACTCGAGCTGGTTTTATAGGACAACAACGCTATGCTGCTATGT GGACTGGTGATAATGTATCAAACTGGGAACATCTCCACATGAGTATAGCCATGGTTCTTCAGTT G

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_016991 						
					
						PMCS
						0.267967707

Conservation Information
Conserved circRNAs	osi_circ_016991
PMCS	0.267967707

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							13541405-13542209(+)
13541423-13542167(-)
						
						Potential amino acid sequence
						
							MVDDLHSVGCKAIWMLDPGIKNESGYFVFDSGLESDVWVQKEDKQPFVGEVWPGDCVFPDFTCE
RARSWWSGLVRQFVSNSVDGLWNDMNEPAVFNTATKTMPESNIHRGDANIGGLQNHPYYHNVYG
MLMAKSTYEGMKLANPTKRPFVLTRAGFIGQQRYAAMWTGDNVSNWEHLHMSIAMVLQLHRFPD
PKCMVDDLHSVGCKAIWMLDPGIKNESGYFVFDSGLESDVWVQKEDKQPFVGEVWPGDCVFPDF
TCERARSWWSGLVRQFVSNSVDGLWNDMNEPAVFNTATKTMPESNIHRGDANIGGLQNHPYYHN
VYGMLMAKSTYEGMKLANPTKRPFVLTRAGFIGQQRYAAMWTGDNVSNWEHLHMSIAMVLQLHR
FPDPKCMVDDLHSVGCKAIWMLDPGIKNESGYFVFDSGLESDVWVQKEDKQPFVGEVWPGDCVF
PDFTCERARSWWSGLVRQFVSNSVDGLWNDMNEPAVFNTATKTMPESNIHRGDANIGGLQNHPY
YHNVYGMLMAKSTYEGMKLANPTKRPFVLTRAGFIGQQRYAAMWTGDNVSNWEHLHMSIAMVLQ
LHRFPDPKCMVDDLHSVGCKAIWMLDPGIKNESGYFVFDSGLESDVWVQKEDKQPFVGEVWPGD
CVFPDFTCERARSWWSGLVRQFVSNSVDGLWNDMNEPAVFNTATKTMPESNIHRGDANIGGLQN
HPYYHNVYGMLMAKSTYEGMKLANPTKRPFVLTRAGFIGQQRYAAMWTGDNVSNWEHLHMSIAM
VLQL(+)
MEIINHTFRIRETMQLKNHGYTHVEMFPV*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	13541405-13542209(+) 13541423-13542167(-)
Potential amino acid sequence	MVDDLHSVGCKAIWMLDPGIKNESGYFVFDSGLESDVWVQKEDKQPFVGEVWPGDCVFPDFTCE RARSWWSGLVRQFVSNSVDGLWNDMNEPAVFNTATKTMPESNIHRGDANIGGLQNHPYYHNVYG MLMAKSTYEGMKLANPTKRPFVLTRAGFIGQQRYAAMWTGDNVSNWEHLHMSIAMVLQLHRFPD PKCMVDDLHSVGCKAIWMLDPGIKNESGYFVFDSGLESDVWVQKEDKQPFVGEVWPGDCVFPDF TCERARSWWSGLVRQFVSNSVDGLWNDMNEPAVFNTATKTMPESNIHRGDANIGGLQNHPYYHN VYGMLMAKSTYEGMKLANPTKRPFVLTRAGFIGQQRYAAMWTGDNVSNWEHLHMSIAMVLQLHR FPDPKCMVDDLHSVGCKAIWMLDPGIKNESGYFVFDSGLESDVWVQKEDKQPFVGEVWPGDCVF PDFTCERARSWWSGLVRQFVSNSVDGLWNDMNEPAVFNTATKTMPESNIHRGDANIGGLQNHPY YHNVYGMLMAKSTYEGMKLANPTKRPFVLTRAGFIGQQRYAAMWTGDNVSNWEHLHMSIAMVLQ LHRFPDPKCMVDDLHSVGCKAIWMLDPGIKNESGYFVFDSGLESDVWVQKEDKQPFVGEVWPGD CVFPDFTCERARSWWSGLVRQFVSNSVDGLWNDMNEPAVFNTATKTMPESNIHRGDANIGGLQN HPYYHNVYGMLMAKSTYEGMKLANPTKRPFVLTRAGFIGQQRYAAMWTGDNVSNWEHLHMSIAM VLQL(+) MEIINHTFRIRETMQLKNHGYTHVEMFPV*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os07g0421300_circ_g.6

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