Plant Circular RNA Database

Detail information of Solyc01g006740.2_circ_g.2

			
						 General Information
					
						CircRNA Name
						Solyc01g006740.2_circ_g.2
					
						ID in PlantcircBase
						sly_circ_000030
					
						Alias
						1:1337381|1337968, Slcirc004
					
						Organism
						
							Solanum lycopersicum						
					
						Position
						
							chr1: 1337381-1337968  JBrowse»						
					
						Reference genome
						
							SL2.50.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						Segemehl, CIRI
					
						Parent gene
						
							Solyc01g006740.2						
					
						Parent gene annotation
						
							protein_coding
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Solyc01g006740.2_circ_g.1 						
					
						Support reads
						43/30
					
						Tissues
						fruit/leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Solyc01g006740.2.1:2

General Information
CircRNA Name	Solyc01g006740.2_circ_g.2
ID in PlantcircBase	sly_circ_000030
Alias	1:1337381\|1337968, Slcirc004
Organism	Solanum lycopersicum
Position	chr1: 1337381-1337968 JBrowse»
Reference genome	SL2.50.38
Type	e-circRNA
Identification method	Segemehl, CIRI
Parent gene	Solyc01g006740.2
Parent gene annotation	protein_coding
Parent gene strand	-
Alternative splicing	Solyc01g006740.2_circ_g.1
Support reads	43/30
Tissues	fruit/leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Solyc01g006740.2.1:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: TACTTTCGGATTATCTTTCGCA
 R: TACCTACACGGGTTGGTTCAGAT
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: TACTTTCGGATTATCTTTCGCA R: TACCTACACGGGTTGGTTCAGAT PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TTATACCCGCAGCACATCTCTCTGTTGCATGAATTACTTTCGGATTATCTTTCGCATTTGCAGC
ATGCCATTGTAATAATTCCTCCTGTGCATTCGATACcgcaaagctcccatttcttgaaacttac
tagccatgaatctgaaccaacccgtgtaggtaagacttgatcaacccaaattcgatattgttta
cccttttt

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 478
Transcript exons: 1337381-1337567,1337678-1337968
CGCAAAGCTCCCATTTCTTGAAACTTACTAGCCATGAATCTGAACCAACCCGTGTAGGTAAGAC
TTGATCAACCCAAATTCGATATTGTTTACCCTTTTTGTCGCCATGACACTTCTTCAATGTATTT
ACGCATTCCTCAAGAGATTTCTCAACTCCAGACGGGTGCACAAAAGTACCAGATGGTCTACAAA
CTGCTTTCAGGTTCGACAAGTAAAGGTCAGAATTCTCTATCTCTCCACGCCTCCATTTCTCGAA
AAACAGGTAAAATTTGACGAATTCATAAGCAGGAACAAAATTCTCCATCTTACTGTCTGACATA
TCGGTAACGTCTCTAGGAGAGGTACTTGGTCCCAGGTTGAAACGCCCAATGGCTTGTATTATAC
CCGCAGCACATCTCTCTGTTGCATGAATTACTTTCGGATTATCTTTCGCATTTGCAGCATGCCA
TTGTAATAATTCCTCCTGTGCATTCGATAC
						
						Genomic sequence
						
							CGCAAAGCTCCCATTTCTTGAAACTTACTAGCCATGAATCTGAACCAACCCGTGTAGGTAAGAC
TTGATCAACCCAAATTCGATATTGTTTACCCTTTTTGTCGCCATGACACTTCTTCAATGTATTT
ACGCATTCCTCAAGAGATTTCTCAACTCCAGACGGGTGCACAAAAGTACCAGATGGTCTCTGCA
GCAACAAAAAGAGTTGGCAAATTTGGATTTTCAAGTGAATAAAAAGGCATTTCGAAACAACCAA
GACAACAACACAACAGTAGTCGGTAGGTATAGGGTACGTACACAAACTGCTTTCAGGTTCGACA
AGTAAAGGTCAGAATTCTCTATCTCTCCACGCCTCCATTTCTCGAAAAACAGGTAAAATTTGAC
GAATTCATAAGCAGGAACAAAATTCTCCATCTTACTGTCTGACATATCGGTAACGTCTCTAGGA
GAGGTACTTGGTCCCAGGTTGAAACGCCCAATGGCTTGTATTATACCCGCAGCACATCTCTCTG
TTGCATGAATTACTTTCGGATTATCTTTCGCATTTGCAGCATGCCATTGTAATAATTCCTCCTG
TGCATTCGATAC

Sequences
Splice junction sequence	TTATACCCGCAGCACATCTCTCTGTTGCATGAATTACTTTCGGATTATCTTTCGCATTTGCAGC ATGCCATTGTAATAATTCCTCCTGTGCATTCGATACcgcaaagctcccatttcttgaaacttac tagccatgaatctgaaccaacccgtgtaggtaagacttgatcaacccaaattcgatattgttta cccttttt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 478 Transcript exons: 1337381-1337567,1337678-1337968 CGCAAAGCTCCCATTTCTTGAAACTTACTAGCCATGAATCTGAACCAACCCGTGTAGGTAAGAC TTGATCAACCCAAATTCGATATTGTTTACCCTTTTTGTCGCCATGACACTTCTTCAATGTATTT ACGCATTCCTCAAGAGATTTCTCAACTCCAGACGGGTGCACAAAAGTACCAGATGGTCTACAAA CTGCTTTCAGGTTCGACAAGTAAAGGTCAGAATTCTCTATCTCTCCACGCCTCCATTTCTCGAA AAACAGGTAAAATTTGACGAATTCATAAGCAGGAACAAAATTCTCCATCTTACTGTCTGACATA TCGGTAACGTCTCTAGGAGAGGTACTTGGTCCCAGGTTGAAACGCCCAATGGCTTGTATTATAC CCGCAGCACATCTCTCTGTTGCATGAATTACTTTCGGATTATCTTTCGCATTTGCAGCATGCCA TTGTAATAATTCCTCCTGTGCATTCGATAC
Genomic sequence	CGCAAAGCTCCCATTTCTTGAAACTTACTAGCCATGAATCTGAACCAACCCGTGTAGGTAAGAC TTGATCAACCCAAATTCGATATTGTTTACCCTTTTTGTCGCCATGACACTTCTTCAATGTATTT ACGCATTCCTCAAGAGATTTCTCAACTCCAGACGGGTGCACAAAAGTACCAGATGGTCTCTGCA GCAACAAAAAGAGTTGGCAAATTTGGATTTTCAAGTGAATAAAAAGGCATTTCGAAACAACCAA GACAACAACACAACAGTAGTCGGTAGGTATAGGGTACGTACACAAACTGCTTTCAGGTTCGACA AGTAAAGGTCAGAATTCTCTATCTCTCCACGCCTCCATTTCTCGAAAAACAGGTAAAATTTGAC GAATTCATAAGCAGGAACAAAATTCTCCATCTTACTGTCTGACATATCGGTAACGTCTCTAGGA GAGGTACTTGGTCCCAGGTTGAAACGCCCAATGGCTTGTATTATACCCGCAGCACATCTCTCTG TTGCATGAATTACTTTCGGATTATCTTTCGCATTTGCAGCATGCCATTGTAATAATTCCTCCTG TGCATTCGATAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.988401162

Conservation Information
Conserved circRNAs	NA
PMCS	0.988401162

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							1337811-1337915(-)
1337417-1337904(-)
						
						Potential amino acid sequence
						
							MSDSKMENFVPAYEFVKFYLFFEKWRRGEIENSDLYLSNLKAVCRPSGTFVHPSGVEKSLEECV
NTLKKCHGDKKGKQYRIWVDQVLPTRVGSDSWLVSFKKWELCGIECTGGIITMACCKCER*(-)
MASKFQEMGALRYRMHRRNYYNGMLQMRKIIRK*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	1337811-1337915(-) 1337417-1337904(-)
Potential amino acid sequence	MSDSKMENFVPAYEFVKFYLFFEKWRRGEIENSDLYLSNLKAVCRPSGTFVHPSGVEKSLEECV NTLKKCHGDKKGKQYRIWVDQVLPTRVGSDSWLVSFKKWELCGIECTGGIITMACCKCER(-) MASKFQEMGALRYRMHRRNYYNGMLQMRKIIRK(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zuo et al., 2016; Tan et al., 2017; Yin et al., 2018; Wang et al., 2018b

circRNA

Detail information of Solyc01g006740.2_circ_g.2

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