Plant Circular RNA Database

Detail information of Zm00001d039693_circ_g.5

			
						 General Information
					
						CircRNA Name
						Zm00001d039693_circ_g.5
					
						ID in PlantcircBase
						zma_circ_007497
					
						Alias
						Zm03circ00011, zma_circ_0001008, GRMZM2G319169_C2
					
						Organism
						
							Zea mays						
					
						Position
						
							chr3: 12077685-12078170  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRI2, find_circ
					
						Parent gene
						
							Zm00001d039693						
					
						Parent gene annotation
						
							Protein RST1
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Zm00001d039693_circ_g.1 Zm00001d039693_circ_g.2 Zm00001d039693_circ_g.3 Zm00001d039693_circ_g.4 						
					
						Support reads
						NA
					
						Tissues
						leaf, endosperm, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d039693_T007:2
Zm00001d039693_T002:2
Zm00001d039693_T055:2
Zm00001d039693_T004:2
Zm00001d039693_T048:2
Zm00001d039693_T035:2
Zm00001d039693_T014:2
Zm00001d039693_T005:2
Zm00001d039693_T016:2
Zm00001d039693_T008:2
Zm00001d039693_T033:2
Zm00001d039693_T064:2
Zm00001d039693_T069:2
Zm00001d039693_T080:2
Zm00001d039693_T036:2
Zm00001d039693_T013:2
Zm00001d039693_T049:2
Zm00001d039693_T047:2
Zm00001d039693_T030:2
Zm00001d039693_T062:2
Zm00001d039693_T082:2
Zm00001d039693_T006:2
Zm00001d039693_T011:2
Zm00001d039693_T053:2
Zm00001d039693_T068:2
Zm00001d039693_T054:2
Zm00001d039693_T001:2
Zm00001d039693_T012:2
Zm00001d039693_T009:2
Zm00001d039693_T074:2
Zm00001d039693_T077:2
Zm00001d039693_T061:2
Zm00001d039693_T070:2
Zm00001d039693_T010:2
Zm00001d039693_T041:2
Zm00001d039693_T079:2
Zm00001d039693_T043:2
Zm00001d039693_T025:2
Zm00001d039693_T073:2
Zm00001d039693_T017:2
Zm00001d039693_T015:2
Zm00001d039693_T065:2
Zm00001d039693_T059:2
Zm00001d039693_T037:2

General Information
CircRNA Name	Zm00001d039693_circ_g.5
ID in PlantcircBase	zma_circ_007497
Alias	Zm03circ00011, zma_circ_0001008, GRMZM2G319169_C2
Organism	Zea mays
Position	chr3: 12077685-12078170 JBrowse»
Reference genome	AGPv4.38
Type	u-circRNA
Identification method	CIRI2, find_circ
Parent gene	Zm00001d039693
Parent gene annotation	Protein RST1
Parent gene strand	+
Alternative splicing	Zm00001d039693_circ_g.1 Zm00001d039693_circ_g.2 Zm00001d039693_circ_g.3 Zm00001d039693_circ_g.4
Support reads	NA
Tissues	leaf, endosperm, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d039693_T007:2 Zm00001d039693_T002:2 Zm00001d039693_T055:2 Zm00001d039693_T004:2 Zm00001d039693_T048:2 Zm00001d039693_T035:2 Zm00001d039693_T014:2 Zm00001d039693_T005:2 Zm00001d039693_T016:2 Zm00001d039693_T008:2 Zm00001d039693_T033:2 Zm00001d039693_T064:2 Zm00001d039693_T069:2 Zm00001d039693_T080:2 Zm00001d039693_T036:2 Zm00001d039693_T013:2 Zm00001d039693_T049:2 Zm00001d039693_T047:2 Zm00001d039693_T030:2 Zm00001d039693_T062:2 Zm00001d039693_T082:2 Zm00001d039693_T006:2 Zm00001d039693_T011:2 Zm00001d039693_T053:2 Zm00001d039693_T068:2 Zm00001d039693_T054:2 Zm00001d039693_T001:2 Zm00001d039693_T012:2 Zm00001d039693_T009:2 Zm00001d039693_T074:2 Zm00001d039693_T077:2 Zm00001d039693_T061:2 Zm00001d039693_T070:2 Zm00001d039693_T010:2 Zm00001d039693_T041:2 Zm00001d039693_T079:2 Zm00001d039693_T043:2 Zm00001d039693_T025:2 Zm00001d039693_T073:2 Zm00001d039693_T017:2 Zm00001d039693_T015:2 Zm00001d039693_T065:2 Zm00001d039693_T059:2 Zm00001d039693_T037:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CAAGCAATGGTCAGCAGCTCTCTCTCTTGGATTAATATTCAATTGTTTTCATCCTACGGACAAA
AAGAGCAGACTTCAGGTTATTAATGGGTTTCTTGAGattctgtgcgaccaagttccggtgtcta
ctcctcaagttgttgttaacatagctcttgttattggggcactggccttggtaaggagtcttta
ttcacatt

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 261
Transcript exons: 12077685-12077762,12077988-12078170
ATTCTGTGCGACCAAGTTCCGGTGTCTACTCCTCAAGTTGTTGTTAACATAGCTCTTGTTATTG
GGGCACTGGCCTTGATTGTTCCGCCAACTGCTCATTTGGTAGTATCTTCGGTATCAGATTTTCT
TTTAAAATGGTTGCTTCAATACGAGCATGAGCACAAGCAATGGTCAGCAGCTCTCTCTCTTGGA
TTAATATTCAATTGTTTTCATCCTACGGACAAAAAGAGCAGACTTCAGGTTATTAATGGGTTTC
TTGAG
						
						Genomic sequence
						
							ATTCTGTGCGACCAAGTTCCGGTGTCTACTCCTCAAGTTGTTGTTAACATAGCTCTTGTTATTG
GGGCACTGGCCTTGGTAAGGAGTCTTTATTCACATTTTATTAATTTCTGAATATATACAGAAAC
TATGGTATGATATAATTGATTTCTCTGCCTAAATTCTCTCCTCCCACCTATGAAAACATTTCAA
GTATCTTTTCCTTGTCACTAGCCCATAAGCTTTGATGTATTCTTATGATTTTACCAGTGGTTGA
GGGATGTTTTACTAATTTTGATTTGTTTGATGTTATCTTATTTCTAGATTGTTCCGCCAACTGC
TCATTTGGTAGTATCTTCGGTATCAGATTTTCTTTTAAAATGGTTGCTTCAATACGAGCATGAG
CACAAGCAATGGTCAGCAGCTCTCTCTCTTGGATTAATATTCAATTGTTTTCATCCTACGGACA
AAAAGAGCAGACTTCAGGTTATTAATGGGTTTCTTGAG

Sequences
Splice junction sequence	CAAGCAATGGTCAGCAGCTCTCTCTCTTGGATTAATATTCAATTGTTTTCATCCTACGGACAAA AAGAGCAGACTTCAGGTTATTAATGGGTTTCTTGAGattctgtgcgaccaagttccggtgtcta ctcctcaagttgttgttaacatagctcttgttattggggcactggccttggtaaggagtcttta ttcacatt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 261 Transcript exons: 12077685-12077762,12077988-12078170 ATTCTGTGCGACCAAGTTCCGGTGTCTACTCCTCAAGTTGTTGTTAACATAGCTCTTGTTATTG GGGCACTGGCCTTGATTGTTCCGCCAACTGCTCATTTGGTAGTATCTTCGGTATCAGATTTTCT TTTAAAATGGTTGCTTCAATACGAGCATGAGCACAAGCAATGGTCAGCAGCTCTCTCTCTTGGA TTAATATTCAATTGTTTTCATCCTACGGACAAAAAGAGCAGACTTCAGGTTATTAATGGGTTTC TTGAG
Genomic sequence	ATTCTGTGCGACCAAGTTCCGGTGTCTACTCCTCAAGTTGTTGTTAACATAGCTCTTGTTATTG GGGCACTGGCCTTGGTAAGGAGTCTTTATTCACATTTTATTAATTTCTGAATATATACAGAAAC TATGGTATGATATAATTGATTTCTCTGCCTAAATTCTCTCCTCCCACCTATGAAAACATTTCAA GTATCTTTTCCTTGTCACTAGCCCATAAGCTTTGATGTATTCTTATGATTTTACCAGTGGTTGA GGGATGTTTTACTAATTTTGATTTGTTTGATGTTATCTTATTTCTAGATTGTTCCGCCAACTGC TCATTTGGTAGTATCTTCGGTATCAGATTTTCTTTTAAAATGGTTGCTTCAATACGAGCATGAG CACAAGCAATGGTCAGCAGCTCTCTCTCTTGGATTAATATTCAATTGTTTTCATCCTACGGACA AAAAGAGCAGACTTCAGGTTATTAATGGGTTTCTTGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.1933971

Conservation Information
Conserved circRNAs	NA
PMCS	0.1933971

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							12078158-12077733(+)
12078010-12078104(-)
						
						Potential amino acid sequence
						
							MGFLRFCATKFRCLLLKLLLT*(+)
MSSWRNNQGQCPNNKSYVNNNLRSRHRNLVAQNLKKPINNLKSALFVRRMKTIEY*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	12078158-12077733(+) 12078010-12078104(-)
Potential amino acid sequence	MGFLRFCATKFRCLLLKLLLT(+) MSSWRNNQGQCPNNKSYVNNNLRSRHRNLVAQNLKKPINNLKSALFVRRMKTIEY(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Han et al., 2020; Ma et al., 2021b;Zhang et al., 2019

circRNA

Detail information of Zm00001d039693_circ_g.5

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