Plant Circular RNA Database

Detail information of Zm00001d022148_circ_g.3

			
						 General Information
					
						CircRNA Name
						Zm00001d022148_circ_g.3
					
						ID in PlantcircBase
						zma_circ_009471
					
						Alias
						zma_circ_0002648
					
						Organism
						
							Zea mays						
					
						Position
						
							chr7: 171255170-171256294  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Zm00001d022148						
					
						Parent gene annotation
						
							 fowlpox viral protein homolog1
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Zm00001d022148_circ_g.1 Zm00001d022148_circ_g.2 						
					
						Support reads
						NA
					
						Tissues
						leaf, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d022148_T001:2
Zm00001d022148_T002:2

General Information
CircRNA Name	Zm00001d022148_circ_g.3
ID in PlantcircBase	zma_circ_009471
Alias	zma_circ_0002648
Organism	Zea mays
Position	chr7: 171255170-171256294 JBrowse»
Reference genome	AGPv4.38
Type	ue-circRNA
Identification method	find_circ
Parent gene	Zm00001d022148
Parent gene annotation	fowlpox viral protein homolog1
Parent gene strand	+
Alternative splicing	Zm00001d022148_circ_g.1 Zm00001d022148_circ_g.2
Support reads	NA
Tissues	leaf, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d022148_T001:2 Zm00001d022148_T002:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							ATACAAAGTTGGCACTGGTCTTGTGGTTGCTCGCAGATCTGATGGATCATGGTCTGCTCCCTCT
GCAATTTTATCTGTTGGCCTGGGATGGGGAGTGCAGgtagtcccttgttcctagtataacctct
aaatgattccatgggaactccaaacgaaatgatggcatactcttccctgtccaaaatggacacc
agccagaa

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 1046
Transcript exons: 171255170-171256014,171256094-171256294
GTAGTCCCTTGTTCCTAGTATAACCTCTAAATGATTCCATGGGAACTCCAAACGAAATGATGGC
ATACTCTTCCCTGTCCAAAATGGACACCAGCCAGAACCACGGAGCTGCTCTGTCCAATGTAGTT
GCTGGAAACAACTATCCTGTTCAGGATTATCTTTATGAGCAAGCATCCGAACCAGACTTCCCAG
AGTATGATTCAAGGGATGACCTGTTCGCTCCAACACGAGCTAGTCCAAAAGTCAATCTAAATAC
TGTTCTAGGTGGTCTGGTTTCGATTGTTACAGGTGTAAACAAGAGTGAGGATGATGCATCGCAG
CATGAAAGTTTTAGCACAGACATATCATTCCTTAGATCTGACAAGAATGGTAATGTCAATGTGC
ACCCTTCCGTCTGTGTTCCAAGTGCCCCACCACTACTTGAAGCGAATGCTTTACAATATAGTGC
ATACAAGGAGGTGTTGCTAGCAGATCCACCAGAGTGGTTGCCAGATAGCTCTGCTAATGTGTGC
CTACAGTGCAACTTGCCTTTCACGGCTCTGACTCGTGGAAGGCACCATTGTCGGTTCTGTGGAG
GTATATTCTGCAAAAACTGTTCCAAAGGAAGATGTTTGATGCCCATGAAATTCAGGATCCGTGA
TCCTCAGAGAGTTTGTGATGCTTGCTATGAGAGGCTTGATCCACTTCAGGGATTATTGATCAAC
TACAATAGTAACTGCATGCAGCAAGCAAAACACGATGTCATGGATTGGACAAGCACTAGAAGCT
GGTTGAACATGCCTGTTGGTGTATCAATGGAGTATGAGATATACAAGGCTACAAACACAATGAA
GAAATATTGTCAGGTTGCTAGATTAAACCCTGAGAAGTCCATCCCATCATCTATTCTCAAGGGG
GCGAAGGGACTTGCAATTCTAACAGTTGCCAAGGCAGGTGCAGTTCTTACATACAAAGTTGGCA
CTGGTCTTGTGGTTGCTCGCAGATCTGATGGATCATGGTCTGCTCCCTCTGCAATTTTATCTGT
TGGCCTGGGATGGGGAGTGCAG
						
					

						Genomic sequence
						
							GTAGTCCCTTGTTCCTAGTATAACCTCTAAATGATTCCATGGGAACTCCAAACGAAATGATGGC
ATACTCTTCCCTGTCCAAAATGGACACCAGCCAGAACCACGGAGCTGCTCTGTCCAATGTAGTT
GCTGGAAACAACTATCCTGTTCAGGATTATCTTTATGAGCAAGCATCCGAACCAGACTTCCCAG
AGTATGATTCAAGGGATGACCTGTTCGCTCCAACACGAGCTAGTCCAAAAGTCAATCTAAATAC
TGTTCTAGGTGGTCTGGTTTCGATTGTTACAGGTGTAAACAAGAGTGAGGATGATGCATCGCAG
CATGAAAGTTTTAGCACAGACATATCATTCCTTAGATCTGACAAGAATGGTAATGTCAATGTGC
ACCCTTCCGTCTGTGTTCCAAGTGCCCCACCACTACTTGAAGCGAATGCTTTACAATATAGTGC
ATACAAGGAGGTGTTGCTAGCAGATCCACCAGAGTGGTTGCCAGATAGCTCTGCTAATGTGTGC
CTACAGTGCAACTTGCCTTTCACGGCTCTGACTCGTGGAAGGCACCATTGTCGGTTCTGTGGAG
GTATATTCTGCAAAAACTGTTCCAAAGGAAGATGTTTGATGCCCATGAAATTCAGGATCCGTGA
TCCTCAGAGAGTTTGTGATGCTTGCTATGAGAGGCTTGATCCACTTCAGGGATTATTGATCAAC
TACAATAGTAACTGCATGCAGCAAGCAAAACACGATGTCATGGATTGGACAAGCACTAGAAGCT
GGTTGAACATGCCTGTTGGTGTATCAATGGAGTATGAGATATACAAGGCTACAAACACAATGAA
GAAATATTGTCAGGTGTGTATGACATTTTAGCTATCAAAAATATGTAACCTTATCATCTAGAGC
TAAGAATTTTCTATTTTGGAATGTGTAGGTTGCTAGATTAAACCCTGAGAAGTCCATCCCATCA
TCTATTCTCAAGGGGGCGAAGGGACTTGCAATTCTAACAGTTGCCAAGGCAGGTGCAGTTCTTA
CATACAAAGTTGGCACTGGTCTTGTGGTTGCTCGCAGATCTGATGGATCATGGTCTGCTCCCTC
TGCAATTTTATCTGTTGGCCTGGGATGGGGAGTGCAG
						
					

			
		

Sequences
Splice junction sequence	ATACAAAGTTGGCACTGGTCTTGTGGTTGCTCGCAGATCTGATGGATCATGGTCTGCTCCCTCT GCAATTTTATCTGTTGGCCTGGGATGGGGAGTGCAGgtagtcccttgttcctagtataacctct aaatgattccatgggaactccaaacgaaatgatggcatactcttccctgtccaaaatggacacc agccagaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 1046 Transcript exons: 171255170-171256014,171256094-171256294 GTAGTCCCTTGTTCCTAGTATAACCTCTAAATGATTCCATGGGAACTCCAAACGAAATGATGGC ATACTCTTCCCTGTCCAAAATGGACACCAGCCAGAACCACGGAGCTGCTCTGTCCAATGTAGTT GCTGGAAACAACTATCCTGTTCAGGATTATCTTTATGAGCAAGCATCCGAACCAGACTTCCCAG AGTATGATTCAAGGGATGACCTGTTCGCTCCAACACGAGCTAGTCCAAAAGTCAATCTAAATAC TGTTCTAGGTGGTCTGGTTTCGATTGTTACAGGTGTAAACAAGAGTGAGGATGATGCATCGCAG CATGAAAGTTTTAGCACAGACATATCATTCCTTAGATCTGACAAGAATGGTAATGTCAATGTGC ACCCTTCCGTCTGTGTTCCAAGTGCCCCACCACTACTTGAAGCGAATGCTTTACAATATAGTGC ATACAAGGAGGTGTTGCTAGCAGATCCACCAGAGTGGTTGCCAGATAGCTCTGCTAATGTGTGC CTACAGTGCAACTTGCCTTTCACGGCTCTGACTCGTGGAAGGCACCATTGTCGGTTCTGTGGAG GTATATTCTGCAAAAACTGTTCCAAAGGAAGATGTTTGATGCCCATGAAATTCAGGATCCGTGA TCCTCAGAGAGTTTGTGATGCTTGCTATGAGAGGCTTGATCCACTTCAGGGATTATTGATCAAC TACAATAGTAACTGCATGCAGCAAGCAAAACACGATGTCATGGATTGGACAAGCACTAGAAGCT GGTTGAACATGCCTGTTGGTGTATCAATGGAGTATGAGATATACAAGGCTACAAACACAATGAA GAAATATTGTCAGGTTGCTAGATTAAACCCTGAGAAGTCCATCCCATCATCTATTCTCAAGGGG GCGAAGGGACTTGCAATTCTAACAGTTGCCAAGGCAGGTGCAGTTCTTACATACAAAGTTGGCA CTGGTCTTGTGGTTGCTCGCAGATCTGATGGATCATGGTCTGCTCCCTCTGCAATTTTATCTGT TGGCCTGGGATGGGGAGTGCAG
Genomic sequence	GTAGTCCCTTGTTCCTAGTATAACCTCTAAATGATTCCATGGGAACTCCAAACGAAATGATGGC ATACTCTTCCCTGTCCAAAATGGACACCAGCCAGAACCACGGAGCTGCTCTGTCCAATGTAGTT GCTGGAAACAACTATCCTGTTCAGGATTATCTTTATGAGCAAGCATCCGAACCAGACTTCCCAG AGTATGATTCAAGGGATGACCTGTTCGCTCCAACACGAGCTAGTCCAAAAGTCAATCTAAATAC TGTTCTAGGTGGTCTGGTTTCGATTGTTACAGGTGTAAACAAGAGTGAGGATGATGCATCGCAG CATGAAAGTTTTAGCACAGACATATCATTCCTTAGATCTGACAAGAATGGTAATGTCAATGTGC ACCCTTCCGTCTGTGTTCCAAGTGCCCCACCACTACTTGAAGCGAATGCTTTACAATATAGTGC ATACAAGGAGGTGTTGCTAGCAGATCCACCAGAGTGGTTGCCAGATAGCTCTGCTAATGTGTGC CTACAGTGCAACTTGCCTTTCACGGCTCTGACTCGTGGAAGGCACCATTGTCGGTTCTGTGGAG GTATATTCTGCAAAAACTGTTCCAAAGGAAGATGTTTGATGCCCATGAAATTCAGGATCCGTGA TCCTCAGAGAGTTTGTGATGCTTGCTATGAGAGGCTTGATCCACTTCAGGGATTATTGATCAAC TACAATAGTAACTGCATGCAGCAAGCAAAACACGATGTCATGGATTGGACAAGCACTAGAAGCT GGTTGAACATGCCTGTTGGTGTATCAATGGAGTATGAGATATACAAGGCTACAAACACAATGAA GAAATATTGTCAGGTGTGTATGACATTTTAGCTATCAAAAATATGTAACCTTATCATCTAGAGC TAAGAATTTTCTATTTTGGAATGTGTAGGTTGCTAGATTAAACCCTGAGAAGTCCATCCCATCA TCTATTCTCAAGGGGGCGAAGGGACTTGCAATTCTAACAGTTGCCAAGGCAGGTGCAGTTCTTA CATACAAAGTTGGCACTGGTCTTGTGGTTGCTCGCAGATCTGATGGATCATGGTCTGCTCCCTC TGCAATTTTATCTGTTGGCCTGGGATGGGGAGTGCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.346772956

Conservation Information
Conserved circRNAs	NA
PMCS	0.346772956

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							171256237-171255170(+)
171256244-171255189(+)
171255209-171255184(+)
171255211-171256193(-)
171255345-171256267(-)
						
						Potential amino acid sequence
						
							MDHGLLPLQFYLLAWDGECR*(+)
MVCSLCNFICWPGMGSAGSPLFLV*(+)
MGTPNEMMAYSSLSKMDTSQNHGAALSNVVAGNNYPVQDYLYEQASEPDFPEYDSRDDLFAPTR
ASPKVNLNTVLGGLVSIVTGVNKSEDDASQHESFSTDISFLRSDKNGNVNVHPSVCVPSAPPLL
EANALQYSAYKEVLLADPPEWLPDSSANVCLQCNLPFTALTRGRHHCRFCGGIFCKNCSKGRCL
MPMKFRIRDPQRVCDACYERLDPLQGLLINYNSNCMQQAKHDVMDWTSTRSWLNMPVGVSMEYE
IYKATNTMKKYCQVARLNPEKSIPSSILKGAKGLAILTVAKAGAVLTYKVGTGLVVARRSDGSW
SAPSAILSVGLGWGVQVVPCS*(+)
MESFRGYTRNKGLPALPIPGQQIKLQREQTMIHQICEQPQDQCQLCM*(-)
MLAHKDNPEQDSCFQQLHWTEQLRGSGWCPFWTGKSMPSFRLEFPWNHLEVILGTRDYLHSPSQ
ANR*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	171256237-171255170(+) 171256244-171255189(+) 171255209-171255184(+) 171255211-171256193(-) 171255345-171256267(-)
Potential amino acid sequence	MDHGLLPLQFYLLAWDGECR(+) MVCSLCNFICWPGMGSAGSPLFLV(+) MGTPNEMMAYSSLSKMDTSQNHGAALSNVVAGNNYPVQDYLYEQASEPDFPEYDSRDDLFAPTR ASPKVNLNTVLGGLVSIVTGVNKSEDDASQHESFSTDISFLRSDKNGNVNVHPSVCVPSAPPLL EANALQYSAYKEVLLADPPEWLPDSSANVCLQCNLPFTALTRGRHHCRFCGGIFCKNCSKGRCL MPMKFRIRDPQRVCDACYERLDPLQGLLINYNSNCMQQAKHDVMDWTSTRSWLNMPVGVSMEYE IYKATNTMKKYCQVARLNPEKSIPSSILKGAKGLAILTVAKAGAVLTYKVGTGLVVARRSDGSW SAPSAILSVGLGWGVQVVPCS(+) MESFRGYTRNKGLPALPIPGQQIKLQREQTMIHQICEQPQDQCQLCM(-) MLAHKDNPEQDSCFQQLHWTEQLRGSGWCPFWTGKSMPSFRLEFPWNHLEVILGTRDYLHSPSQ ANR*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ma et al., 2021b

circRNA

Detail information of Zm00001d022148_circ_g.3

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