Plant Circular RNA Database

Detail information of Os03g0395900_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os03g0395900_circ_g.4
					
						ID in PlantcircBase
						osa_circ_020181
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 15988432-15991466  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os03g0395900						
					
						Parent gene annotation
						
							Hypothetical conserved gene. (Os03t0395900-01);Splicing factor, 
suppressor of white apricot domain containing protein. (Os03t039
5900-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os03g0395900_circ_g.1 Os03g0395900_circ_g.2 Os03g0395900_circ_g.3 Os03g0395900_circ_g.5 Os03g0395900_circ_g.6 						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0395900-01:5
Os03t0395900-02:6

General Information
CircRNA Name	Os03g0395900_circ_g.4
ID in PlantcircBase	osa_circ_020181
Alias	NA
Organism	Oryza sativa
Position	chr3: 15988432-15991466 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os03g0395900
Parent gene annotation	Hypothetical conserved gene. (Os03t0395900-01);Splicing factor, suppressor of white apricot domain containing protein. (Os03t039 5900-02)
Parent gene strand	+
Alternative splicing	Os03g0395900_circ_g.1 Os03g0395900_circ_g.2 Os03g0395900_circ_g.3 Os03g0395900_circ_g.5 Os03g0395900_circ_g.6
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0395900-01:5 Os03t0395900-02:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CATCAGGTGGCCAAATTCTTGAGGCACTTCACAGTGATCCTGCATCTTCTTTGTCTGTGGAGCA
GGAGAAAAGTACAAAAAATTTGAAAGCGCCCACCAGtttctgatgaggcgggtttgcagcatgt
ttcgcaagagttggaggcaaaggctattcttccttttagctttgagaagtaggtctacttttac
tatgcctc

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							TTTCTGATGAGGCGGGTTTGCAGCATGTTTCGCAAGAGTTGGAGGCAAAGGCTATTCTTCCTTT
TAGCTTTGAGAAGTAGGTCTACTTTTACTATGCCTCACTTCTTACATGCGTTTCTTAAGGTTCA
TAACCTATAATTGGAAAAGTTAAGTTGACTGCTTGTGCTTTCTGCAAAGGATGGCAAATCCTAG
CATACATATTTGATGACAACAGACCACAATTACTTTTTGTACTTTGTCAAGCTTATTATCATTC
CTTACACAAAAATATTGGTTAGTAATTTTTGTTCTGGAAGAGCATGTTTGTGTGGCATGTTGTG
AACTTACAAACAAAATATACTGGCAGTTGATGATTATATATGGTGATATCCTTGCAAATTTCTG
GAAACCACAATGTGAAAGCTGTAATTTTTGTCTATATTTTTCACATCTCACAGGACCAGCCGAA
TAAATGCACAGATACCTGTTTTGGTTTGATGTACAAACATGTGCCTACTGCAGAGTGCCCAACT
ATGAAAGAGTCGCCTTCTTTTAATATTTTTCTTAGTATATATTGAATAATTTATTAAAGTGGTA
TTAATATTTCATATAGTTCTAAATTAATATGTGACTACCTGCCTGTCAAAGTATATTCATGTTT
GCCTGAAAATGGAACATTGGTATGTTTAGTCCAACTTTTGGAATTAAAGTTGACATTCACATGT
ATAGTGTGTATGAGACCCCTTATTTGCATAAATTTCTTAGCGTACTTAAGGAAGGAGATATGTA
GGTGCTTATTTTCTGGTGCCTTGACTACTGACTAGCATAAGGCACCGTTCTTCTTTGGGGCAGC
TACTTCCCTGTTTTTTGTTACTTCCTCCGTTTCACAATGTAAGACTTTCTAGCATTGCCCACAT
TCATTTAGATGTTCATGAATCTAGATATATGTATGTGTTTAGATTCATTAACATCTATATGTAT
ATGGGCAATGCTAGAAAGTCTTATATTACAAAACTGAGGGAGTAGTATGCTTCCTAAACTGTTA
AACAATGTTTTTCTTTCAAATAATTTTTATACAGAAGTTTCTTTAAAAAGAAAATCAAATAAAC
CCATTTTCCAAATTTATAATAGCAAATGCTTAATTAATCTTGCGCTAACGATTTTTCTCGTTTC
GCATGCCATCAATAAGCTCAGCCAAAGAGCTGATTCAAACGCTGCCTAAATCAACTATAGGCAA
ATCAGCAAAACATGCTTAAGCTAGGAGCTAGGCAATCACTTGTTTTGTGGTACAACTTGAGTTT
AGGTGTCACATTGTGTAGAGGGTTCCTTTGAGATGTCCTGACTCCTGCGGGATTTAAAATGGTT
ACGCTTATAAGCTTTGGTTATAGAAAAAAGTTTGGTGGACCAAATAATCTCAATTATTAGTAGG
TGCACATGCCAGCAAAAAGGCCAGAAAGTTTATCTTACAAGTTATGGCTCAAAAGCTGGCAAAC
ATTTCTAATTCATGCTGAAATTTTCAGAAGTTTACACATAAGAATTGTAAACGAGCTTATGATT
ATATGCACTGCTTTAGATAGATTTTATTTTTATGAGGCCCAGAATTTCTGTCAATTGGTTCTGT
TTTTGTAGGCCTTCATACAATTTATGTTTTTATCCCTACAATTTTCATTAATAGATGCAGTTTT
TTAGACTTACATTATTTAATTGATTCTATTTTTTTTCACTTGTTTCTGGCAGACCTCAATCCTC
ACAGACCCCTGCTAGCAAAGGCACATATTCACAGGTGGGTTACTCATATAAAGGGGATGGAAAT
GAAGAATCTGAAGATCTAAAGAGTGATGAGGAGGATGAAGAGGAAGAAGATGAAGAGGATGAAA
AGGGCTTCAGTAGTGATGACAGCAGTGATGAACGAATGGAAAGTATAGCCAAGGAATTTGGTGT
AAAACGATATAACTGGCTTGTTTACATGGACAAGAAGGCCAAGGAAGAGGAAAAGCGCCAGAAA
GAGATTATCAAAGGCGATCCTTCCATTGTTAGTACATTTGTAGTTTTAAATATAGCGTATGTTG
CTTGCCTGTAGTGAGAGCTGACAAAATACACTATGCAGAAAAAGCTGAGCCGCAGAGAGAGAAG
AAAAGTTTCTCAAATTGAGAGGGAAAGAGAGCGGGAAGCAGCACGTTCTGTTGGAAGGGTATCT
TATCGTGATCCTTATAGGTAATGTTGCTTGTTCGCTCTATAATTGGGAAGATGAAGTAACAGAA
TGACAAGTATTGTTATTACAATATTCATATGCTCAACATATTAAATTTATAGGGAACAAAGGAG
AAGTCCGTCATATGATTCGTATTCAAGGGGCAGAAGGTGTGCTCAACTTTCACTTTAATTTTTT
AGATGTAAAATCTTTGCGTTGATTAACCTGCAATTATTTCATGATGGCAGATCAAGGTCAAGAT
CAAGATCTCGTTCACCTTCGTATTCTAGACGCCATGGCCGTGGTACACATGCTGAAAGCAACTA
CCGAAGCAAGCCAAAGACTCCAAGGGTTGAATACATCACTGAATTTGGAGGTTCAGATGACACC
AGTGACCTGAAAGTTGCAGGGATATCTCCACCTTCATCTCCAATACGAGTTGGCATACCTAACC
GGTCGGGGAACTCGACCTTTAATATTTGCAAAGATACATTGGGCCATCTTTCTCTGCTGCAGCA
TATTGTCATGGTCCTGGTATTCTAAAAGCTGAACATGTCAAAGAGTAGGATAGTTGAAGACAGG
GTCAAGATGGCATTTATATGCTATAAACTGTAAAATGATATTGGATTTGTAGGTAGACATGTGA
AGCAATAGGTGGCATATGTTTACTGAGCAGGAAGATGATTTAGCTCGTGGAGCTTCTGGCCTAA
GTTTGTTTACTCTTCCAATTCGTTTTGCCTATAATACCATTTCTTTCCTGCAGGTCATCAGGTG
GCCAAATTCTTGAGGCACTTCACAGTGATCCTGCATCTTCTTTGTCTGTGGAGCAGGAGAAAAG
TACAAAAAATTTGAAAGCGCCCACCAG
						
					

			
		

Sequences
Splice junction sequence	CATCAGGTGGCCAAATTCTTGAGGCACTTCACAGTGATCCTGCATCTTCTTTGTCTGTGGAGCA GGAGAAAAGTACAAAAAATTTGAAAGCGCCCACCAGtttctgatgaggcgggtttgcagcatgt ttcgcaagagttggaggcaaaggctattcttccttttagctttgagaagtaggtctacttttac tatgcctc
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	TTTCTGATGAGGCGGGTTTGCAGCATGTTTCGCAAGAGTTGGAGGCAAAGGCTATTCTTCCTTT TAGCTTTGAGAAGTAGGTCTACTTTTACTATGCCTCACTTCTTACATGCGTTTCTTAAGGTTCA TAACCTATAATTGGAAAAGTTAAGTTGACTGCTTGTGCTTTCTGCAAAGGATGGCAAATCCTAG CATACATATTTGATGACAACAGACCACAATTACTTTTTGTACTTTGTCAAGCTTATTATCATTC CTTACACAAAAATATTGGTTAGTAATTTTTGTTCTGGAAGAGCATGTTTGTGTGGCATGTTGTG AACTTACAAACAAAATATACTGGCAGTTGATGATTATATATGGTGATATCCTTGCAAATTTCTG GAAACCACAATGTGAAAGCTGTAATTTTTGTCTATATTTTTCACATCTCACAGGACCAGCCGAA TAAATGCACAGATACCTGTTTTGGTTTGATGTACAAACATGTGCCTACTGCAGAGTGCCCAACT ATGAAAGAGTCGCCTTCTTTTAATATTTTTCTTAGTATATATTGAATAATTTATTAAAGTGGTA TTAATATTTCATATAGTTCTAAATTAATATGTGACTACCTGCCTGTCAAAGTATATTCATGTTT GCCTGAAAATGGAACATTGGTATGTTTAGTCCAACTTTTGGAATTAAAGTTGACATTCACATGT ATAGTGTGTATGAGACCCCTTATTTGCATAAATTTCTTAGCGTACTTAAGGAAGGAGATATGTA GGTGCTTATTTTCTGGTGCCTTGACTACTGACTAGCATAAGGCACCGTTCTTCTTTGGGGCAGC TACTTCCCTGTTTTTTGTTACTTCCTCCGTTTCACAATGTAAGACTTTCTAGCATTGCCCACAT TCATTTAGATGTTCATGAATCTAGATATATGTATGTGTTTAGATTCATTAACATCTATATGTAT ATGGGCAATGCTAGAAAGTCTTATATTACAAAACTGAGGGAGTAGTATGCTTCCTAAACTGTTA AACAATGTTTTTCTTTCAAATAATTTTTATACAGAAGTTTCTTTAAAAAGAAAATCAAATAAAC CCATTTTCCAAATTTATAATAGCAAATGCTTAATTAATCTTGCGCTAACGATTTTTCTCGTTTC GCATGCCATCAATAAGCTCAGCCAAAGAGCTGATTCAAACGCTGCCTAAATCAACTATAGGCAA ATCAGCAAAACATGCTTAAGCTAGGAGCTAGGCAATCACTTGTTTTGTGGTACAACTTGAGTTT AGGTGTCACATTGTGTAGAGGGTTCCTTTGAGATGTCCTGACTCCTGCGGGATTTAAAATGGTT ACGCTTATAAGCTTTGGTTATAGAAAAAAGTTTGGTGGACCAAATAATCTCAATTATTAGTAGG TGCACATGCCAGCAAAAAGGCCAGAAAGTTTATCTTACAAGTTATGGCTCAAAAGCTGGCAAAC ATTTCTAATTCATGCTGAAATTTTCAGAAGTTTACACATAAGAATTGTAAACGAGCTTATGATT ATATGCACTGCTTTAGATAGATTTTATTTTTATGAGGCCCAGAATTTCTGTCAATTGGTTCTGT TTTTGTAGGCCTTCATACAATTTATGTTTTTATCCCTACAATTTTCATTAATAGATGCAGTTTT TTAGACTTACATTATTTAATTGATTCTATTTTTTTTCACTTGTTTCTGGCAGACCTCAATCCTC ACAGACCCCTGCTAGCAAAGGCACATATTCACAGGTGGGTTACTCATATAAAGGGGATGGAAAT GAAGAATCTGAAGATCTAAAGAGTGATGAGGAGGATGAAGAGGAAGAAGATGAAGAGGATGAAA AGGGCTTCAGTAGTGATGACAGCAGTGATGAACGAATGGAAAGTATAGCCAAGGAATTTGGTGT AAAACGATATAACTGGCTTGTTTACATGGACAAGAAGGCCAAGGAAGAGGAAAAGCGCCAGAAA GAGATTATCAAAGGCGATCCTTCCATTGTTAGTACATTTGTAGTTTTAAATATAGCGTATGTTG CTTGCCTGTAGTGAGAGCTGACAAAATACACTATGCAGAAAAAGCTGAGCCGCAGAGAGAGAAG AAAAGTTTCTCAAATTGAGAGGGAAAGAGAGCGGGAAGCAGCACGTTCTGTTGGAAGGGTATCT TATCGTGATCCTTATAGGTAATGTTGCTTGTTCGCTCTATAATTGGGAAGATGAAGTAACAGAA TGACAAGTATTGTTATTACAATATTCATATGCTCAACATATTAAATTTATAGGGAACAAAGGAG AAGTCCGTCATATGATTCGTATTCAAGGGGCAGAAGGTGTGCTCAACTTTCACTTTAATTTTTT AGATGTAAAATCTTTGCGTTGATTAACCTGCAATTATTTCATGATGGCAGATCAAGGTCAAGAT CAAGATCTCGTTCACCTTCGTATTCTAGACGCCATGGCCGTGGTACACATGCTGAAAGCAACTA CCGAAGCAAGCCAAAGACTCCAAGGGTTGAATACATCACTGAATTTGGAGGTTCAGATGACACC AGTGACCTGAAAGTTGCAGGGATATCTCCACCTTCATCTCCAATACGAGTTGGCATACCTAACC GGTCGGGGAACTCGACCTTTAATATTTGCAAAGATACATTGGGCCATCTTTCTCTGCTGCAGCA TATTGTCATGGTCCTGGTATTCTAAAAGCTGAACATGTCAAAGAGTAGGATAGTTGAAGACAGG GTCAAGATGGCATTTATATGCTATAAACTGTAAAATGATATTGGATTTGTAGGTAGACATGTGA AGCAATAGGTGGCATATGTTTACTGAGCAGGAAGATGATTTAGCTCGTGGAGCTTCTGGCCTAA GTTTGTTTACTCTTCCAATTCGTTTTGCCTATAATACCATTTCTTTCCTGCAGGTCATCAGGTG GCCAAATTCTTGAGGCACTTCACAGTGATCCTGCATCTTCTTTGTCTGTGGAGCAGGAGAAAAG TACAAAAAATTTGAAAGCGCCCACCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.132453265

Conservation Information
Conserved circRNAs	NA
PMCS	0.132453265

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							15990324-15988435(+)
15988459-15991052(-)
						
						Potential amino acid sequence
						
							MESIAKEFGVKRYNWLVYMDKKAKEEEKRQKEIIKGDPSIKKLSRRERRKVSQIEREREREAAR
SVGRVSYRDPYREQRRSPSYDSYSRGRRSRSRSRSRSPSYSRRHGRGTHAESNYRSKPKTPRVE
YITEFGGSDDTSDLKVAGISPPSSPIRVGIPNRSSGGQILEALHSDPASSLSVEQEKSTKNLKA
PTSF*(+)
MLQTRLIRNWWALSNFLYFSPAPQTKKMQDHCEVPQEFGHLMTG*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	15990324-15988435(+) 15988459-15991052(-)
Potential amino acid sequence	MESIAKEFGVKRYNWLVYMDKKAKEEEKRQKEIIKGDPSIKKLSRRERRKVSQIEREREREAAR SVGRVSYRDPYREQRRSPSYDSYSRGRRSRSRSRSRSPSYSRRHGRGTHAESNYRSKPKTPRVE YITEFGGSDDTSDLKVAGISPPSSPIRVGIPNRSSGGQILEALHSDPASSLSVEQEKSTKNLKA PTSF(+) MLQTRLIRNWWALSNFLYFSPAPQTKKMQDHCEVPQEFGHLMTG(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os03g0395900_circ_g.4

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