Plant Circular RNA Database

Detail information of Os05g0535900_circ_g.5

			
						 General Information
					
						CircRNA Name
						Os05g0535900_circ_g.5
					
						ID in PlantcircBase
						osa_circ_028671
					
						Alias
						Os_ciR10067
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 26617794-26618481  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Os05g0535900						
					
						Parent gene annotation
						
							IQ calmodulin-binding region domain containing protein. (Os05t05
35900-01);IQ calmodulin-binding region domain containing protein
. (Os05t0535900-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os05g0535900_circ_g.1 Os05g0535900_circ_g.2 Os05g0535900_circ_g.3 Os05g0535900_circ_g.4 Os05g0535900_circ_g.6 						
					
						Support reads
						2
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0535900-01:2
Os05t0535900-02:2

General Information
CircRNA Name	Os05g0535900_circ_g.5
ID in PlantcircBase	osa_circ_028671
Alias	Os_ciR10067
Organism	Oryza sativa
Position	chr5: 26617794-26618481 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	find_circ
Parent gene	Os05g0535900
Parent gene annotation	IQ calmodulin-binding region domain containing protein. (Os05t05 35900-01);IQ calmodulin-binding region domain containing protein . (Os05t0535900-02)
Parent gene strand	+
Alternative splicing	Os05g0535900_circ_g.1 Os05g0535900_circ_g.2 Os05g0535900_circ_g.3 Os05g0535900_circ_g.4 Os05g0535900_circ_g.6
Support reads	2
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0535900-01:2 Os05t0535900-02:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CTAGCAAGATATGCTAGTTATATGATTCTTTCCCCTTTTGCAGAGTGGTAAGCGGGATGCATGG
AAGGAGAAATTGTCTTCAAATGCATTTGCTCGTAAGgcacgtcgggcattccgtgcgttgaaag
gaatcataagactccaagccctgattcgtgggcatctcgtaaggaggcaagccgttgcaactct
tcgtgcaa

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 261
Transcript exons: 26617794-26617997,26618425-26618481
GCACGTCGGGCATTCCGTGCGTTGAAAGGAATCATAAGACTCCAAGCCCTGATTCGTGGGCATC
TCGTAAGGAGGCAAGCCGTTGCAACTCTTCGTGCAACATGGTTGATTGTGAAGTTTCAAGCTCT
AGTCCGTGGTAGGAATGTCAGACTTTCTACTAATACCATTCAAGTCAACTGGAAGCTTGTTCAA
CAACAGTCGGGGAGTGGTAAGCGGGATGCATGGAAGGAGAAATTGTCTTCAAATGCATTTGCTC
GTAAG
						
						Genomic sequence
						
							GCACGTCGGGCATTCCGTGCGTTGAAAGGAATCATAAGACTCCAAGCCCTGATTCGTGGGCATC
TCGTAAGGAGGCAAGCCGTTGCAACTCTTCGTGCAACATGGTTGATTGTGAAGTTTCAAGCTCT
AGTCCGTGGTAGGAATGTCAGACTTTCTACTAATACCATTCAAGTCAACTGGAAGCTTGTTCAA
CAACAGTCGGGGGTAAATTTCTTGCTGATCCTGTTTCATTCAGTACCTGATGTATTCATCATAA
TGATGCCCCTTTCCTTTTAATCTGTTTCACTGTTATATAAAAAAAGGCCTACAAGGCCGCATAC
AAGGAACATGTGCCCCAACCTTTTAGCACTTAAGCCACATAAGCAGCCAATTATGCTGTATAAG
AGCTACAAGGTGGGGTCACCGCACGAAACCGCTTACCACTTTTTAAAACATTGCAAGTCTATCA
TCAAAATGCTCCTGGTTCAACATAAGCAAGTAATAGCACTACAATCATGCTAAATAGAACATTG
CTACACATATTTTTGCATGCTTGCATTCATCGCTTTTAGTTTGTTTGGGAAGCTTTGTTAAAAC
TGACAGATTCGCCTAGCAAGATATGCTAGTTATATGATTCTTTCCCCTTTTGCAGAGTGGTAAG
CGGGATGCATGGAAGGAGAAATTGTCTTCAAATGCATTTGCTCGTAAG

Sequences
Splice junction sequence	CTAGCAAGATATGCTAGTTATATGATTCTTTCCCCTTTTGCAGAGTGGTAAGCGGGATGCATGG AAGGAGAAATTGTCTTCAAATGCATTTGCTCGTAAGgcacgtcgggcattccgtgcgttgaaag gaatcataagactccaagccctgattcgtgggcatctcgtaaggaggcaagccgttgcaactct tcgtgcaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 261 Transcript exons: 26617794-26617997,26618425-26618481 GCACGTCGGGCATTCCGTGCGTTGAAAGGAATCATAAGACTCCAAGCCCTGATTCGTGGGCATC TCGTAAGGAGGCAAGCCGTTGCAACTCTTCGTGCAACATGGTTGATTGTGAAGTTTCAAGCTCT AGTCCGTGGTAGGAATGTCAGACTTTCTACTAATACCATTCAAGTCAACTGGAAGCTTGTTCAA CAACAGTCGGGGAGTGGTAAGCGGGATGCATGGAAGGAGAAATTGTCTTCAAATGCATTTGCTC GTAAG
Genomic sequence	GCACGTCGGGCATTCCGTGCGTTGAAAGGAATCATAAGACTCCAAGCCCTGATTCGTGGGCATC TCGTAAGGAGGCAAGCCGTTGCAACTCTTCGTGCAACATGGTTGATTGTGAAGTTTCAAGCTCT AGTCCGTGGTAGGAATGTCAGACTTTCTACTAATACCATTCAAGTCAACTGGAAGCTTGTTCAA CAACAGTCGGGGGTAAATTTCTTGCTGATCCTGTTTCATTCAGTACCTGATGTATTCATCATAA TGATGCCCCTTTCCTTTTAATCTGTTTCACTGTTATATAAAAAAAGGCCTACAAGGCCGCATAC AAGGAACATGTGCCCCAACCTTTTAGCACTTAAGCCACATAAGCAGCCAATTATGCTGTATAAG AGCTACAAGGTGGGGTCACCGCACGAAACCGCTTACCACTTTTTAAAACATTGCAAGTCTATCA TCAAAATGCTCCTGGTTCAACATAAGCAAGTAATAGCACTACAATCATGCTAAATAGAACATTG CTACACATATTTTTGCATGCTTGCATTCATCGCTTTTAGTTTGTTTGGGAAGCTTTGTTAAAAC TGACAGATTCGCCTAGCAAGATATGCTAGTTATATGATTCTTTCCCCTTTTGCAGAGTGGTAAG CGGGATGCATGGAAGGAGAAATTGTCTTCAAATGCATTTGCTCGTAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.263960453

Conservation Information
Conserved circRNAs	NA
PMCS	0.263960453

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							26617937-26617815(+)
26617858-26618463(-)
26617830-26617984(-)
26617808-26617968(-)
						
						Potential amino acid sequence
						
							MSDFLLIPFKSTGSLFNNSRGVVSGMHGRRNCLQMHLLVRHVGHSVR*(+)
MPTNQGLESYDSFQRTECPTCLTSKCI*(-)
MIPFNARNARRALRANAFEDNFSFHASRLPLPDCC*(-)
MPDVPYEQMHLKTISPSMHPAYHSPTVVEQASS*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	26617937-26617815(+) 26617858-26618463(-) 26617830-26617984(-) 26617808-26617968(-)
Potential amino acid sequence	MSDFLLIPFKSTGSLFNNSRGVVSGMHGRRNCLQMHLLVRHVGHSVR(+) MPTNQGLESYDSFQRTECPTCLTSKCI(-) MIPFNARNARRALRANAFEDNFSFHASRLPLPDCC(-) MPDVPYEQMHLKTISPSMHPAYHSPTVVEQASS(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of Os05g0535900_circ_g.5

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