Plant Circular RNA Database

Detail information of AT3G63450_circ_g.8

			
						 General Information
					
						CircRNA Name
						AT3G63450_circ_g.8
					
						ID in PlantcircBase
						ath_circ_028964
					
						Alias
						AT3G63450_C1, AT3G63450_C1
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr3: 23427656-23428178  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI2
					
						Parent gene
						
							AT3G63450						
					
						Parent gene annotation
						
							RNA-binding (RRM/RBD/RNP motifs) family protein
							
						Parent gene strand
						-
					
						Alternative splicing
						
							AT3G63450_circ_g.3 AT3G63450_circ_g.4 AT3G63450_circ_g.5 AT3G63450_circ_g.6 AT3G63450_circ_g.7 AT3G63450_circ_g.9 AT3G63450_circ_g.10 						
					
						Support reads
						NA
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT3G63450.2:3
AT3G63450.1:3
AT3G63450.7:3
AT3G63450.3:3
AT3G63450.6:3
AT3G63450.4:3
AT3G63450.5:3

General Information
CircRNA Name	AT3G63450_circ_g.8
ID in PlantcircBase	ath_circ_028964
Alias	AT3G63450_C1, AT3G63450_C1
Organism	Arabidpsis thaliana
Position	chr3: 23427656-23428178 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRI2
Parent gene	AT3G63450
Parent gene annotation	RNA-binding (RRM/RBD/RNP motifs) family protein
Parent gene strand	-
Alternative splicing	AT3G63450_circ_g.3 AT3G63450_circ_g.4 AT3G63450_circ_g.5 AT3G63450_circ_g.6 AT3G63450_circ_g.7 AT3G63450_circ_g.9 AT3G63450_circ_g.10
Support reads	NA
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT3G63450.2:3 AT3G63450.1:3 AT3G63450.7:3 AT3G63450.3:3 AT3G63450.6:3 AT3G63450.4:3 AT3G63450.5:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CAGAATGCTCTTCACAGTCTCTGGGTACATGAAGGTCACGAACCCAAACATCCTCTTCTGCTGG
TAAGGTATCCTCACGTCCTGAACTGGCCCAAACGTActagtcgtggagaaacaagtagtgtttg
gtcgaaattgagttgaaactgtttctcgacgtcgagaagctgcagattcatcagccttgtgctc
tgcagctc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 383
Transcript exons: 23427656-23427814,23427881-23427918,23427993-23428178
CTAGTCGTGGAGAAACAAGTAGTGTTTGGTCGAAATTGAGTTGAAACTGTTTCTCGACGTCGAG
AAGCTGCAGATTCATCAGCCTTGTGCTCTGCAGCTCAAGAGCTCGTTCTTCAAACCTCCTTTTC
CACAGCAGATCTTCATGAAAAGCCCTCCCCCCAAGCTGGAAATCCATAATGTCCCTGGATGGCT
TAGTCCTGTACTTGTCTGGGACTTTGCCTTTCTCCTTGTAAGGCTTAACAAGAACCCTGGAATG
ACACACAAAGTGAGGGTTCCCTTTGGCCAGAATGCTCTTCACAGTCTCTGGGTACATGAAGGTC
ACGAACCCAAACATCCTCTTCTGCTGGTAAGGTATCCTCACGTCCTGAACTGGCCCAAACGTA
						
						Genomic sequence
						
							CTAGTCGTGGAGAAACAAGTAGTGTTTGGTCGAAATTGAGTTGAAACTGTTTCTCGACGTCGAG
AAGCTGCAGATTCATCAGCCTTGTGCTCTGCAGCTCAAGAGCTCGTTCTTCAAACCTCCTTTTC
CACAGCAGATCTTCATGAAAAGCCCTCCCCCCTATACACAGAGAAATCAGTATTCAATACCAAG
GGAAATAAAAGAGAGAGAGAGAGAGCTTTATACCAAGCTGGAAATCCATAATGTCCCTGGATGG
CTTAGTCCTGCAGCAGAGGAATCAAAGCGTCGTAAAGAGATGAGGAATCAGGAGCAGCTCAAGG
AGGAAAAGCCAAAGTACCTGTACTTGTCTGGGACTTTGCCTTTCTCCTTGTAAGGCTTAACAAG
AACCCTGGAATGACACACAAAGTGAGGGTTCCCTTTGGCCAGAATGCTCTTCACAGTCTCTGGG
TACATGAAGGTCACGAACCCAAACATCCTCTTCTGCTGGTAAGGTATCCTCACGTCCTGAACTG
GCCCAAACGTA

Sequences
Splice junction sequence	CAGAATGCTCTTCACAGTCTCTGGGTACATGAAGGTCACGAACCCAAACATCCTCTTCTGCTGG TAAGGTATCCTCACGTCCTGAACTGGCCCAAACGTActagtcgtggagaaacaagtagtgtttg gtcgaaattgagttgaaactgtttctcgacgtcgagaagctgcagattcatcagccttgtgctc tgcagctc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 383 Transcript exons: 23427656-23427814,23427881-23427918,23427993-23428178 CTAGTCGTGGAGAAACAAGTAGTGTTTGGTCGAAATTGAGTTGAAACTGTTTCTCGACGTCGAG AAGCTGCAGATTCATCAGCCTTGTGCTCTGCAGCTCAAGAGCTCGTTCTTCAAACCTCCTTTTC CACAGCAGATCTTCATGAAAAGCCCTCCCCCCAAGCTGGAAATCCATAATGTCCCTGGATGGCT TAGTCCTGTACTTGTCTGGGACTTTGCCTTTCTCCTTGTAAGGCTTAACAAGAACCCTGGAATG ACACACAAAGTGAGGGTTCCCTTTGGCCAGAATGCTCTTCACAGTCTCTGGGTACATGAAGGTC ACGAACCCAAACATCCTCTTCTGCTGGTAAGGTATCCTCACGTCCTGAACTGGCCCAAACGTA
Genomic sequence	CTAGTCGTGGAGAAACAAGTAGTGTTTGGTCGAAATTGAGTTGAAACTGTTTCTCGACGTCGAG AAGCTGCAGATTCATCAGCCTTGTGCTCTGCAGCTCAAGAGCTCGTTCTTCAAACCTCCTTTTC CACAGCAGATCTTCATGAAAAGCCCTCCCCCCTATACACAGAGAAATCAGTATTCAATACCAAG GGAAATAAAAGAGAGAGAGAGAGAGCTTTATACCAAGCTGGAAATCCATAATGTCCCTGGATGG CTTAGTCCTGCAGCAGAGGAATCAAAGCGTCGTAAAGAGATGAGGAATCAGGAGCAGCTCAAGG AGGAAAAGCCAAAGTACCTGTACTTGTCTGGGACTTTGCCTTTCTCCTTGTAAGGCTTAACAAG AACCCTGGAATGACACACAAAGTGAGGGTTCCCTTTGGCCAGAATGCTCTTCACAGTCTCTGGG TACATGAAGGTCACGAACCCAAACATCCTCTTCTGCTGGTAAGGTATCCTCACGTCCTGAACTG GCCCAAACGTA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.349881807

Conservation Information
Conserved circRNAs	NA
PMCS	0.349881807

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							23428131-23428155(-)
						
						Potential amino acid sequence
						
							MFGFVTFMYPETVKSILAKGNPHFVCHSRVLVKPYKEKGKVPDKYRTKPSRDIMDFQLGGRAFH
EDLLWKRRFEERALELQSTRLMNLQLLDVEKQFQLNFDQTLLVSPRLVRLGQFRT*
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						response to drought stress

Functional Information
Coding potential	Y
Potential coding position	23428131-23428155(-)
Potential amino acid sequence	MFGFVTFMYPETVKSILAKGNPHFVCHSRVLVKPYKEKGKVPDKYRTKPSRDIMDFQLGGRAFH EDLLWKRRFEERALELQSTRLMNLQLLDVEKQFQLNFDQTLLVSPRLVRLGQFRT*
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	response to drought stress

			
						 Other Information
					
						References
						Zhang et al., 2019

circRNA

Detail information of AT3G63450_circ_g.8

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