Plant Circular RNA Database

Detail information of AT3G44970_circ_g.2

			
						 General Information
					
						CircRNA Name
						AT3G44970_circ_g.2
					
						ID in PlantcircBase
						ath_circ_026109
					
						Alias
						At_ciR4129
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr3: 16433169-16434006  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder, circRNA_finder, find_circ
					
						Parent gene
						
							AT3G44970						
					
						Parent gene annotation
						
							Cytochrome P450 superfamily protein
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT3G44970_circ_g.1 AT3G44970_circ_g.3 AT3G44970_circ_g.4 						
					
						Support reads
						4/6/2
					
						Tissues
						leaf/whole_plant/seedlings
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT3G44970.2:3
AT3G44970.1:3

General Information
CircRNA Name	AT3G44970_circ_g.2
ID in PlantcircBase	ath_circ_026109
Alias	At_ciR4129
Organism	Arabidpsis thaliana
Position	chr3: 16433169-16434006 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder, circRNA_finder, find_circ
Parent gene	AT3G44970
Parent gene annotation	Cytochrome P450 superfamily protein
Parent gene strand	+
Alternative splicing	AT3G44970_circ_g.1 AT3G44970_circ_g.3 AT3G44970_circ_g.4
Support reads	4/6/2
Tissues	leaf/whole_plant/seedlings
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT3G44970.2:3 AT3G44970.1:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCGGCAGAAAGAACATGAGGTGATCCTTGAAAGCAGAGAAGACAAAGAAGGTGGAGTTACTTGG
GAAGAATACAGACACAAGATGACTTTCACCAACATGttgataatagcccacttgacacctaaga
tgatgagtaatctcaaaccacagactcaagcaaagcttatgggaatttttaaggccttcacttt
tgattggt

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 495
Transcript exons: 16433169-16433321,16433414-16433662,16433914-16434006
TTGATAATAGCCCACTTGACACCTAAGATGATGAGTAATCTCAAACCACAGACTCAAGCAAAGC
TTATGGGAATTTTTAAGGCCTTCACTTTTGATTGGTTTCGGACATCCTATCTTATCTCTGCCGG
GAAGGGTCTCTACAACACCCTTTGGGCATGCCGAGAGGGGATGCGAGAGATAAAGGATATATAT
ACAATGAGGAAAACGTCTGAGGAGAAGTACGATGACTTCCTCAACACGGCAATAGAAGAGTCAG
AGAAAGCAGGAGAGTTATTGAACGAAAATGCAATTATAACCCTCATCTTTACCCTTTCATGTGT
CACTCAAGATACCACCTCAAAGGCGATTTGCTTGGCTGTGAAATTCCTATTGGAAAACCCAAAA
GTTCTCGCAGAGTTAAAGAAAGAACATGAGGTGATCCTTGAAAGCAGAGAAGACAAAGAAGGTG
GAGTTACTTGGGAAGAATACAGACACAAGATGACTTTCACCAACATG
						
						Genomic sequence
						
							TTGATAATAGCCCACTTGACACCTAAGATGATGAGTAATCTCAAACCACAGACTCAAGCAAAGC
TTATGGGAATTTTTAAGGCCTTCACTTTTGATTGGTTTCGGACATCCTATCTTATCTCTGCCGG
GAAGGGTCTCTACAACACCCTTTGGGTGCGATTTTTTTTTCTCTTTATTTCTTTCCTTAGGAGT
CACGGTAAGTATCAAACTAACACGAATCCATGATCTCTTAATGTTAAATGCAGGCATGCCGAGA
GGGGATGCGAGAGATAAAGGATATATATACAATGAGGAAAACGTCTGAGGAGAAGTACGATGAC
TTCCTCAACACGGCAATAGAAGAGTCAGAGAAAGCAGGAGAGTTATTGAACGAAAATGCAATTA
TAACCCTCATCTTTACCCTTTCATGTGTCACTCAAGATACCACCTCAAAGGCGATTTGCTTGGC
TGTGAAATTCCTATTGGAAAACCCAAAAGTTCTCGCAGAGTTAAAGGTAATATTTACATACAGA
TGTATAAGATGGTAAATTTTACATATAACCATGTGTTAGTATCGACCATGACATGTAGTAGTTT
TTGCTAGTGATGTTGGTGTGTCACAATATGGATTCAAATCCGCATATTGTATGTTGTTATTGTT
AATAAAAAAATTATGCTAATCTGGTAGTCATGGTCCTGTTAAGTAGGTTCAAGATCTTTTGCAT
TTTGTATTGAGAAAATCATATTTATATCAATAATTCGGCAGAAAGAACATGAGGTGATCCTTGA
AAGCAGAGAAGACAAAGAAGGTGGAGTTACTTGGGAAGAATACAGACACAAGATGACTTTCACC
AACATG

Sequences
Splice junction sequence	TCGGCAGAAAGAACATGAGGTGATCCTTGAAAGCAGAGAAGACAAAGAAGGTGGAGTTACTTGG GAAGAATACAGACACAAGATGACTTTCACCAACATGttgataatagcccacttgacacctaaga tgatgagtaatctcaaaccacagactcaagcaaagcttatgggaatttttaaggccttcacttt tgattggt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 495 Transcript exons: 16433169-16433321,16433414-16433662,16433914-16434006 TTGATAATAGCCCACTTGACACCTAAGATGATGAGTAATCTCAAACCACAGACTCAAGCAAAGC TTATGGGAATTTTTAAGGCCTTCACTTTTGATTGGTTTCGGACATCCTATCTTATCTCTGCCGG GAAGGGTCTCTACAACACCCTTTGGGCATGCCGAGAGGGGATGCGAGAGATAAAGGATATATAT ACAATGAGGAAAACGTCTGAGGAGAAGTACGATGACTTCCTCAACACGGCAATAGAAGAGTCAG AGAAAGCAGGAGAGTTATTGAACGAAAATGCAATTATAACCCTCATCTTTACCCTTTCATGTGT CACTCAAGATACCACCTCAAAGGCGATTTGCTTGGCTGTGAAATTCCTATTGGAAAACCCAAAA GTTCTCGCAGAGTTAAAGAAAGAACATGAGGTGATCCTTGAAAGCAGAGAAGACAAAGAAGGTG GAGTTACTTGGGAAGAATACAGACACAAGATGACTTTCACCAACATG
Genomic sequence	TTGATAATAGCCCACTTGACACCTAAGATGATGAGTAATCTCAAACCACAGACTCAAGCAAAGC TTATGGGAATTTTTAAGGCCTTCACTTTTGATTGGTTTCGGACATCCTATCTTATCTCTGCCGG GAAGGGTCTCTACAACACCCTTTGGGTGCGATTTTTTTTTCTCTTTATTTCTTTCCTTAGGAGT CACGGTAAGTATCAAACTAACACGAATCCATGATCTCTTAATGTTAAATGCAGGCATGCCGAGA GGGGATGCGAGAGATAAAGGATATATATACAATGAGGAAAACGTCTGAGGAGAAGTACGATGAC TTCCTCAACACGGCAATAGAAGAGTCAGAGAAAGCAGGAGAGTTATTGAACGAAAATGCAATTA TAACCCTCATCTTTACCCTTTCATGTGTCACTCAAGATACCACCTCAAAGGCGATTTGCTTGGC TGTGAAATTCCTATTGGAAAACCCAAAAGTTCTCGCAGAGTTAAAGGTAATATTTACATACAGA TGTATAAGATGGTAAATTTTACATATAACCATGTGTTAGTATCGACCATGACATGTAGTAGTTT TTGCTAGTGATGTTGGTGTGTCACAATATGGATTCAAATCCGCATATTGTATGTTGTTATTGTT AATAAAAAAATTATGCTAATCTGGTAGTCATGGTCCTGTTAAGTAGGTTCAAGATCTTTTGCAT TTTGTATTGAGAAAATCATATTTATATCAATAATTCGGCAGAAAGAACATGAGGTGATCCTTGA AAGCAGAGAAGACAAAGAAGGTGGAGTTACTTGGGAAGAATACAGACACAAGATGACTTTCACC AACATG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.184252178

Conservation Information
Conserved circRNAs	NA
PMCS	0.184252178

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							16433196-16434003(+)
						
						Potential amino acid sequence
						
							MMSNLKPQTQAKLMGIFKAFTFDWFRTSYLISAGKGLYNTLWACREGMREIKDIYTMRKTSEEK
YDDFLNTAIEESEKAGELLNENAIITLIFTLSCVTQDTTSKAICLAVKFLLENPKVLAELKKEH
EVILESREDKEGGVTWEEYRHKMTFTNMLIIAHLTPKMMSNLKPQTQAKLMGIFKAFTFDWFRT
SYLISAGKGLYNTLWACREGMREIKDIYTMRKTSEEKYDDFLNTAIEESEKAGELLNENAIITL
IFTLSCVTQDTTSKAICLAVKFLLENPKVLAELKKEHEVILESREDKEGGVTWEEYRHKMTFTN
MLIIAHLTPKMMSNLKPQTQAKLMGIFKAFTFDWFRTSYLISAGKGLYNTLWACREGMREIKDI
YTMRKTSEEKYDDFLNTAIEESEKAGELLNENAIITLIFTLSCVTQDTTSKAICLAVKFLLENP
KVLAELKKEHEVILESREDKEGGVTWEEYRHKMTFTNMLIIAHLTPKMMSNLKPQTQAKLMGIF
KAFTFDWFRTSYLISAGKGLYNTLWACREGMREIKDIYTMRKTSEEKYDDFLNTAIEESEKAGE
LLNENAIITLIFTLSCVTQDTTSKAICLAVKFLLENPKVLAELKKEHEVILESREDKEGGVTWE
EYRHKMTFTNM(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	16433196-16434003(+)
Potential amino acid sequence	MMSNLKPQTQAKLMGIFKAFTFDWFRTSYLISAGKGLYNTLWACREGMREIKDIYTMRKTSEEK YDDFLNTAIEESEKAGELLNENAIITLIFTLSCVTQDTTSKAICLAVKFLLENPKVLAELKKEH EVILESREDKEGGVTWEEYRHKMTFTNMLIIAHLTPKMMSNLKPQTQAKLMGIFKAFTFDWFRT SYLISAGKGLYNTLWACREGMREIKDIYTMRKTSEEKYDDFLNTAIEESEKAGELLNENAIITL IFTLSCVTQDTTSKAICLAVKFLLENPKVLAELKKEHEVILESREDKEGGVTWEEYRHKMTFTN MLIIAHLTPKMMSNLKPQTQAKLMGIFKAFTFDWFRTSYLISAGKGLYNTLWACREGMREIKDI YTMRKTSEEKYDDFLNTAIEESEKAGELLNENAIITLIFTLSCVTQDTTSKAICLAVKFLLENP KVLAELKKEHEVILESREDKEGGVTWEEYRHKMTFTNMLIIAHLTPKMMSNLKPQTQAKLMGIF KAFTFDWFRTSYLISAGKGLYNTLWACREGMREIKDIYTMRKTSEEKYDDFLNTAIEESEKAGE LLNENAIITLIFTLSCVTQDTTSKAICLAVKFLLENPKVLAELKKEHEVILESREDKEGGVTWE EYRHKMTFTNM(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017;Pan et al., 2017

circRNA

Detail information of AT3G44970_circ_g.2

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