Plant Circular RNA Database

Detail information of Os05g0188500_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os05g0188500_circ_g.2
					
						ID in PlantcircBase
						osa_circ_026902
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 5428828-5429767  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os05g0188500						
					
						Parent gene annotation
						
							Similar to SCC3. (Os05t0188500-01);Armadillo-type fold domain co
ntaining protein. (Os05t0188500-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os05g0188500_circ_g.3 						
					
						Support reads
						1
					
						Tissues
						shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0188500-02:2
Os05t0188500-01:1

General Information
CircRNA Name	Os05g0188500_circ_g.2
ID in PlantcircBase	osa_circ_026902
Alias	NA
Organism	Oryza sativa
Position	chr5: 5428828-5429767 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os05g0188500
Parent gene annotation	Similar to SCC3. (Os05t0188500-01);Armadillo-type fold domain co ntaining protein. (Os05t0188500-02)
Parent gene strand	+
Alternative splicing	Os05g0188500_circ_g.3
Support reads	1
Tissues	shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0188500-02:2 Os05t0188500-01:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TTTAAGAAAATACATATCTGACAAAGCTAAGATATCACCTCTAATTGATATGATGATGCTTATG
AAACTTGAGCTGTACTCACTAAAAAGGCAAGATCAGgattggaagtgtataatctccatgcttc
tggatgaaaatccattaaccgagcttactgacatggatggaaccaatctagttcggatgctgcg
agcctctg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 295
Transcript exons: 5428828-5428960,5429606-5429767
GATTGGAAGTGTATAATCTCCATGCTTCTGGATGAAAATCCATTAACCGAGCTTACTGACATGG
ATGGAACCAATCTAGTTCGGATGCTGCGAGCCTCTGCAAAGAAGGCTGTTGGGGAAAGGATAGT
TCCTGGAAATATTGGAAAACAGCAAGCATGAAATAACTACTGCTTTGCTGAAGAAATATCCACA
GCTTTTAAGAAAATACATATCTGACAAAGCTAAGATATCACCTCTAATTGATATGATGATGCTT
ATGAAACTTGAGCTGTACTCACTAAAAAGGCAAGATCAG
Transcript length: 336
Transcript exons: 5428828-5429001,5429606-5429767
GATTGGAAGTGTATAATCTCCATGCTTCTGGATGAAAATCCATTAACCGAGCTTACTGACATGG
ATGGAACCAATCTAGTTCGGATGCTGCGAGCCTCTGCAAAGAAGGCTGTTGGGGAAAGGATAGT
TCCTGCAACAGACAATAGAAAGATGTACTACAATAAAGGCCAGAAGGAAATATTGGAAAACAGC
AAGCATGAAATAACTACTGCTTTGCTGAAGAAATATCCACAGCTTTTAAGAAAATACATATCTG
ACAAAGCTAAGATATCACCTCTAATTGATATGATGATGCTTATGAAACTTGAGCTGTACTCACT
AAAAAGGCAAGATCAG
						
					

						Genomic sequence
						
							GATTGGAAGTGTATAATCTCCATGCTTCTGGATGAAAATCCATTAACCGAGCTTACTGACATGG
ATGGAACCAATCTAGTTCGGATGCTGCGAGCCTCTGCAAAGAAGGCTGTTGGGGAAAGGATAGT
TCCTGCAACAGACAATAGAAAGATGTACTACAATAAAGGCCAGAAGGTGTCAATGTCATTTCTG
TCGTATCATATGTTGCTGCTGCAAGTTGATTGTTTATTTCTTGATCGTATCATTTGTTGTATTG
CTTGTTTAGTGTTGCTGCCTTAGTGGTTATTTAGGACTTGTAAGTACTATGCGTAGAATGTAGG
TTTATTTTGTTGGATATTGGCAATGTCCTACACCTTTGAAAGTTAGTCTGACAAGTTAATCTCT
TAAAAGACCTGTTTGCTAGATACATCCATTTTTAAATATTACACTGTTAGACTTTATTTGGTCA
AACTTTTGAAACATTGACCATCAATATCTCTCAAAATATTTTGTTTGGGAACATAAAAATCATA
TTGGTAGTTTTTTTCTTTAATTGTACTTTTACAATATTAATTTTTTTAGATATTATTGTTATAT
TCTAATAGAAAATAGTGGTCAAAGTTGCACACCCAAGACCAAAGAAAATCAACAGTGTCATCTA
TTTAAATACGGAGGTAGTAATTGCTAGTCATGTAGATTGTAGTTCTGAATCTGTTGCCTGCTTG
TTGAATCTGGTTTGTTTGTCGTACCATTGTTCATGTATTTGTTGAATTGTTACTGAAGCATTAT
TATTCTGCAGGAAATATTGGAAAACAGCAAGCATGAAATAACTACTGCTTTGCTGAAGAAATAT
CCACAGCTTTTAAGAAAATACATATCTGACAAAGCTAAGATATCACCTCTAATTGATATGATGA
TGCTTATGAAACTTGAGCTGTACTCACTAAAAAGGCAAGATCAG
						
					

			
		

Sequences
Splice junction sequence	TTTAAGAAAATACATATCTGACAAAGCTAAGATATCACCTCTAATTGATATGATGATGCTTATG AAACTTGAGCTGTACTCACTAAAAAGGCAAGATCAGgattggaagtgtataatctccatgcttc tggatgaaaatccattaaccgagcttactgacatggatggaaccaatctagttcggatgctgcg agcctctg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 295 Transcript exons: 5428828-5428960,5429606-5429767 GATTGGAAGTGTATAATCTCCATGCTTCTGGATGAAAATCCATTAACCGAGCTTACTGACATGG ATGGAACCAATCTAGTTCGGATGCTGCGAGCCTCTGCAAAGAAGGCTGTTGGGGAAAGGATAGT TCCTGGAAATATTGGAAAACAGCAAGCATGAAATAACTACTGCTTTGCTGAAGAAATATCCACA GCTTTTAAGAAAATACATATCTGACAAAGCTAAGATATCACCTCTAATTGATATGATGATGCTT ATGAAACTTGAGCTGTACTCACTAAAAAGGCAAGATCAG Transcript length: 336 Transcript exons: 5428828-5429001,5429606-5429767 GATTGGAAGTGTATAATCTCCATGCTTCTGGATGAAAATCCATTAACCGAGCTTACTGACATGG ATGGAACCAATCTAGTTCGGATGCTGCGAGCCTCTGCAAAGAAGGCTGTTGGGGAAAGGATAGT TCCTGCAACAGACAATAGAAAGATGTACTACAATAAAGGCCAGAAGGAAATATTGGAAAACAGC AAGCATGAAATAACTACTGCTTTGCTGAAGAAATATCCACAGCTTTTAAGAAAATACATATCTG ACAAAGCTAAGATATCACCTCTAATTGATATGATGATGCTTATGAAACTTGAGCTGTACTCACT AAAAAGGCAAGATCAG
Genomic sequence	GATTGGAAGTGTATAATCTCCATGCTTCTGGATGAAAATCCATTAACCGAGCTTACTGACATGG ATGGAACCAATCTAGTTCGGATGCTGCGAGCCTCTGCAAAGAAGGCTGTTGGGGAAAGGATAGT TCCTGCAACAGACAATAGAAAGATGTACTACAATAAAGGCCAGAAGGTGTCAATGTCATTTCTG TCGTATCATATGTTGCTGCTGCAAGTTGATTGTTTATTTCTTGATCGTATCATTTGTTGTATTG CTTGTTTAGTGTTGCTGCCTTAGTGGTTATTTAGGACTTGTAAGTACTATGCGTAGAATGTAGG TTTATTTTGTTGGATATTGGCAATGTCCTACACCTTTGAAAGTTAGTCTGACAAGTTAATCTCT TAAAAGACCTGTTTGCTAGATACATCCATTTTTAAATATTACACTGTTAGACTTTATTTGGTCA AACTTTTGAAACATTGACCATCAATATCTCTCAAAATATTTTGTTTGGGAACATAAAAATCATA TTGGTAGTTTTTTTCTTTAATTGTACTTTTACAATATTAATTTTTTTAGATATTATTGTTATAT TCTAATAGAAAATAGTGGTCAAAGTTGCACACCCAAGACCAAAGAAAATCAACAGTGTCATCTA TTTAAATACGGAGGTAGTAATTGCTAGTCATGTAGATTGTAGTTCTGAATCTGTTGCCTGCTTG TTGAATCTGGTTTGTTTGTCGTACCATTGTTCATGTATTTGTTGAATTGTTACTGAAGCATTAT TATTCTGCAGGAAATATTGGAAAACAGCAAGCATGAAATAACTACTGCTTTGCTGAAGAAATAT CCACAGCTTTTAAGAAAATACATATCTGACAAAGCTAAGATATCACCTCTAATTGATATGATGA TGCTTATGAAACTTGAGCTGTACTCACTAAAAAGGCAAGATCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_006020* osi_circ_015982 						
					
						PMCS
						0.369756578

Conservation Information
Conserved circRNAs	osi_circ_006020* osi_circ_015982
PMCS	0.369756578

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							5428850-5429764(+)
5428891-5429766(-)
5428872-5429729(-)
						
						Potential amino acid sequence
						
							MLLDENPLTELTDMDGTNLVRMLRASAKKAVGERIVPATDNRKMYYNKGQKEILENSKHEITTA
LLKKYPQLLRKYISDKAKISPLIDMMMLMKLELYSLKRQDQDWKCIISMLLDENPLTELTDMDG
TNLVRMLRASAKKAVGERIVPATDNRKMYYNKGQKEILENSKHEITTALLKKYPQLLRKYISDK
AKISPLIDMMMLMKLELYSLKRQDQDWKCIISMLLDENPLTELTDMDGTNLVRMLRASAKKAVG
ERIVPATDNRKMYYNKGQKEILENSKHEITTALLKKYPQLLRKYISDKAKISPLIDMMMLMKLE
LYSLKRQDQDWKCIISMLLDENPLTELTDMDGTNLVRMLRASAKKAVGERIVPATDNRKMYYNK
GQKEILENSKHEITTALLKKYPQLLRKYISDKAKISPLIDMMMLMKLELYSLKRQDQ(+)
MSVSSVNGFSSRSMEIIHFQS*(-)
MDFHPEAWRLYTSNPDLAFLVSTAQVS*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	5428850-5429764(+) 5428891-5429766(-) 5428872-5429729(-)
Potential amino acid sequence	MLLDENPLTELTDMDGTNLVRMLRASAKKAVGERIVPATDNRKMYYNKGQKEILENSKHEITTA LLKKYPQLLRKYISDKAKISPLIDMMMLMKLELYSLKRQDQDWKCIISMLLDENPLTELTDMDG TNLVRMLRASAKKAVGERIVPATDNRKMYYNKGQKEILENSKHEITTALLKKYPQLLRKYISDK AKISPLIDMMMLMKLELYSLKRQDQDWKCIISMLLDENPLTELTDMDGTNLVRMLRASAKKAVG ERIVPATDNRKMYYNKGQKEILENSKHEITTALLKKYPQLLRKYISDKAKISPLIDMMMLMKLE LYSLKRQDQDWKCIISMLLDENPLTELTDMDGTNLVRMLRASAKKAVGERIVPATDNRKMYYNK GQKEILENSKHEITTALLKKYPQLLRKYISDKAKISPLIDMMMLMKLELYSLKRQDQ(+) MSVSSVNGFSSRSMEIIHFQS(-) MDFHPEAWRLYTSNPDLAFLVSTAQVS(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os05g0188500_circ_g.2

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