Plant Circular RNA Database

Detail information of Os03g0680800_circ_g.14

			
						 General Information
					
						CircRNA Name
						Os03g0680800_circ_g.14
					
						ID in PlantcircBase
						osa_circ_020963
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 27060351-27062053  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os03g0680800						
					
						Parent gene annotation
						
							Similar to cDNA clone:J023038C04, full insert sequence. (Os03t06
80800-01);Similar to Prep (Fragment). (Os03t0680800-02);Similar 
to Associated with HOX family protein, expressed. (Os03t0680800-
03);Similar to cDNA clone:J023038C04, full insert sequence. (Os0
3t0680800-04);Similar to cDNA clone:J023038C04, full insert sequ
ence. (Os03t0680800-05)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						3
					
						Tissues
						shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0680800-03:2
Os03t0680800-01:2
Os03t0680800-05:2
Os03t0680800-02:2

General Information
CircRNA Name	Os03g0680800_circ_g.14
ID in PlantcircBase	osa_circ_020963
Alias	NA
Organism	Oryza sativa
Position	chr3: 27060351-27062053 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer
Parent gene	Os03g0680800
Parent gene annotation	Similar to cDNA clone:J023038C04, full insert sequence. (Os03t06 80800-01);Similar to Prep (Fragment). (Os03t0680800-02);Similar to Associated with HOX family protein, expressed. (Os03t0680800- 03);Similar to cDNA clone:J023038C04, full insert sequence. (Os0 3t0680800-04);Similar to cDNA clone:J023038C04, full insert sequ ence. (Os03t0680800-05)
Parent gene strand	-
Alternative splicing	NA
Support reads	3
Tissues	shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0680800-03:2 Os03t0680800-01:2 Os03t0680800-05:2 Os03t0680800-02:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TTGGATCAATTGATTGATGGCAGAAGAATGCATCCGAGTCGTCAGAGCTCCTTCATCAGCTCAG
CGACGCGGTAGGAGGGTTCCTTGCTTTGTAATCACActcatccaacatggccataagttttgcc
atcttattctggagctcttgtttctcagcagcagaaatctctggtgcagcattggcagtagact
cctgtggg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 1051
Transcript exons: 27060351-27061297,27061950-27062053
CTCATCCAACATGGCCATAAGTTTTGCCATCTTATTCTGGAGCTCTTGTTTCTCAGCAGCAGAA
ATCTCTGGTGCAGCATTGGCAGTAGACTCCTGTGGGTTGGAAGATACCCCCTCACCTTTCGAAC
CCCCCTCGGCTTCTTTGTTATCTGATTTTCCTGCTTCAGCCTGGTCTTTCTGAGCCTTCTGCTT
TATACTCTTCCAAACACTGACGACCTCATCAAGCAATTCTTGTGCTGCTTTCAGATACTTTGAG
TTTCTGATTGCCTGTGAGGCCTCAGATTGCATATTCTTCATTCTGTTGTCATCACCATGGTAAG
AGTGTGGTGTTAGCAACTCTGTTTTCGCAGACCAGTATGGATACGAAGGCGCCAGGATATGCGT
GTTGAGGCTCAGAGACAATCCTTGGCCTGGTGCTGGTGCAACGCTCATCGAATTGTGGTTGTTC
AATAGGCCAAACTCAATCTGCGCGCTGCTATGAGTGTCATTGTGGATGAGATCAGCACCAGCAT
TATGTGCTCCATCCATCAGATGCATCAACATCTCATTTCTCATATCTTTGGCAGGTCCAAAAGA
GCGCTGATCCATGTACGACGCGACCATGTTAGGTTCCCGTGACGATGAATCTTGAGAGATCGCA
GTTGGATGGCCAGGCAGCTCCATGAAATTCTGCTGATGCTGGATAATGCCACTGAATTCCGTGT
ATGGCCCAGAAGACCCATTGCCAGGATATAACATGTTTCCTATTGCTGATGGCACAGGATATGA
TGAATTGCCTGAATCCGCTGGGTACATAGCTTGCGAGTCCCTTTCATTGCCAGGGCTCGAGTAG
TAAGTAGCCATGTATGCAGCTTTGACAACTCAATTAGCAAGATTGCTGCAAAGGCATGGATACT
TGCAGGGAATCAATTACAGAGTTCACCAAACTCAAATTCCGATCGAGTTGCCTTGTTGGATCAA
TTGATTGATGGCAGAAGAATGCATCCGAGTCGTCAGAGCTCCTTCATCAGCTCAGCGACGCGGT
AGGAGGGTTCCTTGCTTTGTAATCACA
						
					

						Genomic sequence
						
							CTCATCCAACATGGCCATAAGTTTTGCCATCTTATTCTGGAGCTCTTGTTTCTCAGCAGCAGAA
ATCTCTGGTGCAGCATTGGCAGTAGACTCCTGTGGGTTGGAAGATACCCCCTCACCTTTCGAAC
CCCCCTCGGCTTCTTTGTTATCTGATTTTCCTGCTTCAGCCTGGTCTTTCTGAGCCTTCTGCTT
TATACTCTTCCAAACACTGACGACCTCATCAAGCAATTCTTGTGCTGCTTTCAGATACTTTGAG
TTTCTGATTGCCTGTGAGGCCTCAGATTGCATATTCTTCATTCTGTTGTCATCACCATGGTAAG
AGTGTGGTGTTAGCAACTCTGTTTTCGCAGACCAGTATGGATACGAAGGCGCCAGGATATGCGT
GTTGAGGCTCAGAGACAATCCTTGGCCTGGTGCTGGTGCAACGCTCATCGAATTGTGGTTGTTC
AATAGGCCAAACTCAATCTGCGCGCTGCTATGAGTGTCATTGTGGATGAGATCAGCACCAGCAT
TATGTGCTCCATCCATCAGATGCATCAACATCTCATTTCTCATATCTTTGGCAGGTCCAAAAGA
GCGCTGATCCATGTACGACGCGACCATGTTAGGTTCCCGTGACGATGAATCTTGAGAGATCGCA
GTTGGATGGCCAGGCAGCTCCATGAAATTCTGCTGATGCTGGATAATGCCACTGAATTCCGTGT
ATGGCCCAGAAGACCCATTGCCAGGATATAACATGTTTCCTATTGCTGATGGCACAGGATATGA
TGAATTGCCTGAATCCGCTGGGTACATAGCTTGCGAGTCCCTTTCATTGCCAGGGCTCGAGTAG
TAAGTAGCCATGTATGCAGCTTTGACAACTCAATTAGCAAGATTGCTGCAAAGGCATGGATACT
TGCAGGGAATCAATTACAGAGTTCACCAAACTCAAATTCCGATCGAGTTGCCTGTCAAAGGGGG
AAGGAAAAAGGTTCAACAAAACAATGACAACAAAAAGGTACAGTTAGGAATTATATATGAGACA
ATCAAATTCTACACCACATAATGAAATAGTTAAGAAGAAACAAAACACACTATAATCACCAGAT
CTTTCTTTCTTTTTTTTTTTTGGGAAAGCAAGTACGGTTCTGTTACTATTGCAAGGACACAAGA
TACACACAAAGGCCAGTCACGAGTGAGTGAGGCTCAGACCCACAGCTTTGGCAGCAGTTACCAC
CTCTGTTTTGTTCTTTTCTTTCTTACTATATTTGTAGGGAAGAACACAGGTTATATGCTCAAGA
AACAAGAGAATATAAACTATGAAGTGACAAGGTAAGATGCCAGTTTGCTGGCTAAAGGTACAGA
ACTTCAGGTGGTCCTTCATCTGAGTTTATAGCTAGGTTATTGGCCTTTTGCTCCTTCCTTATCA
AGATAATCAAGACGGCGAGAATGTGGCTATAAATTCTTCCTATTTCATCAAGATTAGGCGACCT
TTCAAGAACCCAATGAGATAAGTGGTTCTATACCACATACAAGAACTGCAAGGCCACAGATAAA
ATTGTAGGGTTTTTTTTTCTTGAGAAAAAGAAAGAACTTATAAATGGAATACTATTTCAATACC
TTGTTGGATCAATTGATTGATGGCAGAAGAATGCATCCGAGTCGTCAGAGCTCCTTCATCAGCT
CAGCGACGCGGTAGGAGGGTTCCTTGCTTTGTAATCACA
						
					

			
		

Sequences
Splice junction sequence	TTGGATCAATTGATTGATGGCAGAAGAATGCATCCGAGTCGTCAGAGCTCCTTCATCAGCTCAG CGACGCGGTAGGAGGGTTCCTTGCTTTGTAATCACActcatccaacatggccataagttttgcc atcttattctggagctcttgtttctcagcagcagaaatctctggtgcagcattggcagtagact cctgtggg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 1051 Transcript exons: 27060351-27061297,27061950-27062053 CTCATCCAACATGGCCATAAGTTTTGCCATCTTATTCTGGAGCTCTTGTTTCTCAGCAGCAGAA ATCTCTGGTGCAGCATTGGCAGTAGACTCCTGTGGGTTGGAAGATACCCCCTCACCTTTCGAAC CCCCCTCGGCTTCTTTGTTATCTGATTTTCCTGCTTCAGCCTGGTCTTTCTGAGCCTTCTGCTT TATACTCTTCCAAACACTGACGACCTCATCAAGCAATTCTTGTGCTGCTTTCAGATACTTTGAG TTTCTGATTGCCTGTGAGGCCTCAGATTGCATATTCTTCATTCTGTTGTCATCACCATGGTAAG AGTGTGGTGTTAGCAACTCTGTTTTCGCAGACCAGTATGGATACGAAGGCGCCAGGATATGCGT GTTGAGGCTCAGAGACAATCCTTGGCCTGGTGCTGGTGCAACGCTCATCGAATTGTGGTTGTTC AATAGGCCAAACTCAATCTGCGCGCTGCTATGAGTGTCATTGTGGATGAGATCAGCACCAGCAT TATGTGCTCCATCCATCAGATGCATCAACATCTCATTTCTCATATCTTTGGCAGGTCCAAAAGA GCGCTGATCCATGTACGACGCGACCATGTTAGGTTCCCGTGACGATGAATCTTGAGAGATCGCA GTTGGATGGCCAGGCAGCTCCATGAAATTCTGCTGATGCTGGATAATGCCACTGAATTCCGTGT ATGGCCCAGAAGACCCATTGCCAGGATATAACATGTTTCCTATTGCTGATGGCACAGGATATGA TGAATTGCCTGAATCCGCTGGGTACATAGCTTGCGAGTCCCTTTCATTGCCAGGGCTCGAGTAG TAAGTAGCCATGTATGCAGCTTTGACAACTCAATTAGCAAGATTGCTGCAAAGGCATGGATACT TGCAGGGAATCAATTACAGAGTTCACCAAACTCAAATTCCGATCGAGTTGCCTTGTTGGATCAA TTGATTGATGGCAGAAGAATGCATCCGAGTCGTCAGAGCTCCTTCATCAGCTCAGCGACGCGGT AGGAGGGTTCCTTGCTTTGTAATCACA
Genomic sequence	CTCATCCAACATGGCCATAAGTTTTGCCATCTTATTCTGGAGCTCTTGTTTCTCAGCAGCAGAA ATCTCTGGTGCAGCATTGGCAGTAGACTCCTGTGGGTTGGAAGATACCCCCTCACCTTTCGAAC CCCCCTCGGCTTCTTTGTTATCTGATTTTCCTGCTTCAGCCTGGTCTTTCTGAGCCTTCTGCTT TATACTCTTCCAAACACTGACGACCTCATCAAGCAATTCTTGTGCTGCTTTCAGATACTTTGAG TTTCTGATTGCCTGTGAGGCCTCAGATTGCATATTCTTCATTCTGTTGTCATCACCATGGTAAG AGTGTGGTGTTAGCAACTCTGTTTTCGCAGACCAGTATGGATACGAAGGCGCCAGGATATGCGT GTTGAGGCTCAGAGACAATCCTTGGCCTGGTGCTGGTGCAACGCTCATCGAATTGTGGTTGTTC AATAGGCCAAACTCAATCTGCGCGCTGCTATGAGTGTCATTGTGGATGAGATCAGCACCAGCAT TATGTGCTCCATCCATCAGATGCATCAACATCTCATTTCTCATATCTTTGGCAGGTCCAAAAGA GCGCTGATCCATGTACGACGCGACCATGTTAGGTTCCCGTGACGATGAATCTTGAGAGATCGCA GTTGGATGGCCAGGCAGCTCCATGAAATTCTGCTGATGCTGGATAATGCCACTGAATTCCGTGT ATGGCCCAGAAGACCCATTGCCAGGATATAACATGTTTCCTATTGCTGATGGCACAGGATATGA TGAATTGCCTGAATCCGCTGGGTACATAGCTTGCGAGTCCCTTTCATTGCCAGGGCTCGAGTAG TAAGTAGCCATGTATGCAGCTTTGACAACTCAATTAGCAAGATTGCTGCAAAGGCATGGATACT TGCAGGGAATCAATTACAGAGTTCACCAAACTCAAATTCCGATCGAGTTGCCTGTCAAAGGGGG AAGGAAAAAGGTTCAACAAAACAATGACAACAAAAAGGTACAGTTAGGAATTATATATGAGACA ATCAAATTCTACACCACATAATGAAATAGTTAAGAAGAAACAAAACACACTATAATCACCAGAT CTTTCTTTCTTTTTTTTTTTTGGGAAAGCAAGTACGGTTCTGTTACTATTGCAAGGACACAAGA TACACACAAAGGCCAGTCACGAGTGAGTGAGGCTCAGACCCACAGCTTTGGCAGCAGTTACCAC CTCTGTTTTGTTCTTTTCTTTCTTACTATATTTGTAGGGAAGAACACAGGTTATATGCTCAAGA AACAAGAGAATATAAACTATGAAGTGACAAGGTAAGATGCCAGTTTGCTGGCTAAAGGTACAGA ACTTCAGGTGGTCCTTCATCTGAGTTTATAGCTAGGTTATTGGCCTTTTGCTCCTTCCTTATCA AGATAATCAAGACGGCGAGAATGTGGCTATAAATTCTTCCTATTTCATCAAGATTAGGCGACCT TTCAAGAACCCAATGAGATAAGTGGTTCTATACCACATACAAGAACTGCAAGGCCACAGATAAA ATTGTAGGGTTTTTTTTTCTTGAGAAAAAGAAAGAACTTATAAATGGAATACTATTTCAATACC TTGTTGGATCAATTGATTGATGGCAGAAGAATGCATCCGAGTCGTCAGAGCTCCTTCATCAGCT CAGCGACGCGGTAGGAGGGTTCCTTGCTTTGTAATCACA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.292365668

Conservation Information
Conserved circRNAs	NA
PMCS	0.292365668

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							27061971-27060500(+)
27061195-27062011(-)
27060432-27062016(-)
						
						Potential amino acid sequence
						
							MAEECIRVVRAPSSAQRRGRRVPCFVITLIQHGHKFCHLILELLFLSSRNLWCSIGSRLLWVGR
YPLTFRTPLGFFVI*(+)
MATYYSSPGNERDSQAMYPADSGNSSYPVPSAIGNMLYPGNGSSGPYTEFSGIIQHQQNFMELP
GHPTAISQDSSSREPNMVASYMDQRSFGPAKDMRNEMLMHLMDGAHNAGADLIHNDTHSSAQIE
FGLLNNHNSMSVAPAPGQGLSLSLNTHILAPSYPYWSAKTELLTPHSYHGDDNRMKNMQSEASQ
AIRNSKYLKAAQELLDEVVSVWKSIKQKAQKDQAEAGKSDNKEAEGGSKGEGVSSNPQESTANA
APEISAAEKQELQNKMAKLMAMLDECDYKARNPPTASLS*(-)
MLHQRFLLLRNKSSRIRWQNLWPCWMSVITKQGTLLPRR*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	27061971-27060500(+) 27061195-27062011(-) 27060432-27062016(-)
Potential amino acid sequence	MAEECIRVVRAPSSAQRRGRRVPCFVITLIQHGHKFCHLILELLFLSSRNLWCSIGSRLLWVGR YPLTFRTPLGFFVI(+) MATYYSSPGNERDSQAMYPADSGNSSYPVPSAIGNMLYPGNGSSGPYTEFSGIIQHQQNFMELP GHPTAISQDSSSREPNMVASYMDQRSFGPAKDMRNEMLMHLMDGAHNAGADLIHNDTHSSAQIE FGLLNNHNSMSVAPAPGQGLSLSLNTHILAPSYPYWSAKTELLTPHSYHGDDNRMKNMQSEASQ AIRNSKYLKAAQELLDEVVSVWKSIKQKAQKDQAEAGKSDNKEAEGGSKGEGVSSNPQESTANA APEISAAEKQELQNKMAKLMAMLDECDYKARNPPTASLS(-) MLHQRFLLLRNKSSRIRWQNLWPCWMSVITKQGTLLPRR*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os03g0680800_circ_g.14

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