Plant Circular RNA Database

Detail information of Os02g0131800_circ_g.5

			
						 General Information
					
						CircRNA Name
						Os02g0131800_circ_g.5
					
						ID in PlantcircBase
						osa_circ_012984
					
						Alias
						Os_ciR4386
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 1660458-1661231  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os02g0131800						
					
						Parent gene annotation
						
							Trivalent AI influx transporter, Aluminum (Al) tolerance (Os02t0
131800-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os02g0131800_circ_g.6 						
					
						Support reads
						5/5
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0131800-01:4

General Information
CircRNA Name	Os02g0131800_circ_g.5
ID in PlantcircBase	osa_circ_012984
Alias	Os_ciR4386
Organism	Oryza sativa
Position	chr2: 1660458-1661231 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os02g0131800
Parent gene annotation	Trivalent AI influx transporter, Aluminum (Al) tolerance (Os02t0 131800-01)
Parent gene strand	-
Alternative splicing	Os02g0131800_circ_g.6
Support reads	5/5
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0131800-01:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TAATGGTTAAGTGAAATATACCTGTTTTCACTCCAAGGTTGGCAGCTAATGTTTGGATCAGAAG
TGCAAAGACCATCCCAACTAAGATGACCCACAGAAGggtgtgatgatagcgccaaagagtgcga
tcgcattggcagcagcgcctttcccttggagagaagggacaaacatgcccttgaccacctctcc
tgcagacg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 202
Transcript exons: 1660458-1660523,1660525-1660625,1661197-1661231
GGTGTGATGATAGCGCCAAAGAGTGCGATCGCATTGGCAGCAGCGCCTTTCCCTTGGAGAGAAG
GGCAAACATGCCCTTGACCACCTCTCCTGCAGACGGCCTCAAATAGCTTAGCTCTCCGAAGAAG
CAAGCTGCCATAGTGAACATGAATGCTGCTATGATGAACGCAAAGACCATCCCAACTAAGATGA
CCCACAGAAG
Transcript length: 476
Transcript exons: 1660458-1660639,1660738-1660847,1660948-1661052,1661153-
1661231
GGTGTGATGATAGCGCCAAAGAGTGCGATCGCATTGGCAGCAGCGCCTTTCCCTTGGAGAGAAG
GGACAAACATGCCCTTGACCACCTCTCCTGCAGACGGCCTCAAATAGCTTAGCTCTCCGAAGAA
GCAAGCTGCCATAGTGAACATGAATGCTGCTATGATGAACTCCAGCTTCCGAGCCCCAAATCTC
TGTACTCCAAGAAGCAAGAGGGTGCTGAACACTGTGAGAATAACTCCAGCCCACACTGGAATCT
TAAGCAATATGTTGAACGCAAAAGCTGTGCCCAACACTTCAGGAATGTCATCAGAAATCACCGC
CAACTCCGCAATGATCCATAAGAAAATGTTGACATAGTGCGGGTACTCTTCCCTGCACAGTTCA
GCAAGGTGCCTTCCTGTTTTCACTCCAAGGTTGGCAGCTAATGTTTGGATCAGAAGTGCAAAGA
CCATCCCAACTAAGATGACCCACAGAAG
						
					

						Genomic sequence
						
							GGTGTGATGATAGCGCCAAAGAGTGCGATCGCATTGGCAGCAGCGCCTTTCCCTTGGAGAGAAG
GGACAAACATGCCCTTGACCACCTCTCCTGCAGACGGCCTCAAATAGCTTAGCTCTCCGAAGAA
GCAAGCTGCCATAGTGAACATGAATGCTGCTATGATGAACTCCAGCTTCCGAGCCTGGTGGACA
TGTCATATGGTAAGGTCAATTCGTCAGTAGCCCATTCTCAGTGTAATTCATCTGACAATCATAT
AAGTCTGAGAAATTCTTTGCGCACCCCAAATCTCTGTACTCCAAGAAGCAAGAGGGTGCTGAAC
ACTGTGAGAATAACTCCAGCCCACACTGGAATCTTAAGCAATATGTTGAACGCAAAAGCTGTGC
CCAACACTGCACTAACAAAAGAACGTGTTACTCATGCTAAATCTAGCACAGAAATAAATAAATA
AATAAATAAATAAATAAATAAATAAATAAAACATCGATCAACCTTCAGGAATGTCATCAGAAAT
CACCGCCAACTCCGCAATGATCCATAAGAAAATGTTGACATAGTGCGGGTACTCTTCCCTGCAC
AGTTCAGCAAGGTGCCTTCCTGTACAGAACTCAACAAAAGCATGGACAACATAAGATGATAAGG
TGATGATGCATGATTAGCATGCAAAGAGCAAGGTTAATGGTTAAGTGAAATATACCTGTTTTCA
CTCCAAGGTTGGCAGCTAATGTTTGGATCAGAAGTGCAAAGACCATCCCAACTAAGATGACCCA
CAGAAG
						
					

			
		

Sequences
Splice junction sequence	TAATGGTTAAGTGAAATATACCTGTTTTCACTCCAAGGTTGGCAGCTAATGTTTGGATCAGAAG TGCAAAGACCATCCCAACTAAGATGACCCACAGAAGggtgtgatgatagcgccaaagagtgcga tcgcattggcagcagcgcctttcccttggagagaagggacaaacatgcccttgaccacctctcc tgcagacg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 202 Transcript exons: 1660458-1660523,1660525-1660625,1661197-1661231 GGTGTGATGATAGCGCCAAAGAGTGCGATCGCATTGGCAGCAGCGCCTTTCCCTTGGAGAGAAG GGCAAACATGCCCTTGACCACCTCTCCTGCAGACGGCCTCAAATAGCTTAGCTCTCCGAAGAAG CAAGCTGCCATAGTGAACATGAATGCTGCTATGATGAACGCAAAGACCATCCCAACTAAGATGA CCCACAGAAG Transcript length: 476 Transcript exons: 1660458-1660639,1660738-1660847,1660948-1661052,1661153- 1661231 GGTGTGATGATAGCGCCAAAGAGTGCGATCGCATTGGCAGCAGCGCCTTTCCCTTGGAGAGAAG GGACAAACATGCCCTTGACCACCTCTCCTGCAGACGGCCTCAAATAGCTTAGCTCTCCGAAGAA GCAAGCTGCCATAGTGAACATGAATGCTGCTATGATGAACTCCAGCTTCCGAGCCCCAAATCTC TGTACTCCAAGAAGCAAGAGGGTGCTGAACACTGTGAGAATAACTCCAGCCCACACTGGAATCT TAAGCAATATGTTGAACGCAAAAGCTGTGCCCAACACTTCAGGAATGTCATCAGAAATCACCGC CAACTCCGCAATGATCCATAAGAAAATGTTGACATAGTGCGGGTACTCTTCCCTGCACAGTTCA GCAAGGTGCCTTCCTGTTTTCACTCCAAGGTTGGCAGCTAATGTTTGGATCAGAAGTGCAAAGA CCATCCCAACTAAGATGACCCACAGAAG
Genomic sequence	GGTGTGATGATAGCGCCAAAGAGTGCGATCGCATTGGCAGCAGCGCCTTTCCCTTGGAGAGAAG GGACAAACATGCCCTTGACCACCTCTCCTGCAGACGGCCTCAAATAGCTTAGCTCTCCGAAGAA GCAAGCTGCCATAGTGAACATGAATGCTGCTATGATGAACTCCAGCTTCCGAGCCTGGTGGACA TGTCATATGGTAAGGTCAATTCGTCAGTAGCCCATTCTCAGTGTAATTCATCTGACAATCATAT AAGTCTGAGAAATTCTTTGCGCACCCCAAATCTCTGTACTCCAAGAAGCAAGAGGGTGCTGAAC ACTGTGAGAATAACTCCAGCCCACACTGGAATCTTAAGCAATATGTTGAACGCAAAAGCTGTGC CCAACACTGCACTAACAAAAGAACGTGTTACTCATGCTAAATCTAGCACAGAAATAAATAAATA AATAAATAAATAAATAAATAAATAAATAAAACATCGATCAACCTTCAGGAATGTCATCAGAAAT CACCGCCAACTCCGCAATGATCCATAAGAAAATGTTGACATAGTGCGGGTACTCTTCCCTGCAC AGTTCAGCAAGGTGCCTTCCTGTACAGAACTCAACAAAAGCATGGACAACATAAGATGATAAGG TGATGATGCATGATTAGCATGCAAAGAGCAAGGTTAATGGTTAAGTGAAATATACCTGTTTTCA CTCCAAGGTTGGCAGCTAATGTTTGGATCAGAAGTGCAAAGACCATCCCAACTAAGATGACCCA CAGAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.270511466

Conservation Information
Conserved circRNAs	NA
PMCS	0.270511466

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							1661209-1661215(-)
1660493-1661039(-)
						
						Potential amino acid sequence
						
							MVFALLIQTLAANLGVKTGRHLAELCREEYPHYVNIFLWIIAELAVISDDIPEVLGTAFAFNIL
LKIPVWAGVILTVFSTLLLLGVQRFGARKLEFIIAAFMFTMAACFFGELSYLRPSAGEVVKGMF
VPSLQGKGAAANAIALFGAIITPFCGSS*(-)
MRSHSLALSSHPSVGHLSWDGLCTSDPNISCQPWSENRKAPC*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	1661209-1661215(-) 1660493-1661039(-)
Potential amino acid sequence	MVFALLIQTLAANLGVKTGRHLAELCREEYPHYVNIFLWIIAELAVISDDIPEVLGTAFAFNIL LKIPVWAGVILTVFSTLLLLGVQRFGARKLEFIIAAFMFTMAACFFGELSYLRPSAGEVVKGMF VPSLQGKGAAANAIALFGAIITPFCGSS(-) MRSHSLALSSHPSVGHLSWDGLCTSDPNISCQPWSENRKAPC(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os02g0131800_circ_g.5

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