Plant Circular RNA Database

Detail information of Os03g0197000_circ_g.6

			
						 General Information
					
						CircRNA Name
						Os03g0197000_circ_g.6
					
						ID in PlantcircBase
						osa_circ_018368
					
						Alias
						Os_ciR9080
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 5087401-5088363  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os03g0197000						
					
						Parent gene annotation
						
							Di-trans-poly-cis-decaprenylcistransferase-like domain containin
g protein. (Os03t0197000-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os03g0197000_circ_g.2 Os03g0197000_circ_g.3 Os03g0197000_circ_g.4 Os03g0197000_circ_g.5 						
					
						Support reads
						2/1
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0197000-01:3

General Information
CircRNA Name	Os03g0197000_circ_g.6
ID in PlantcircBase	osa_circ_018368
Alias	Os_ciR9080
Organism	Oryza sativa
Position	chr3: 5087401-5088363 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os03g0197000
Parent gene annotation	Di-trans-poly-cis-decaprenylcistransferase-like domain containin g protein. (Os03t0197000-01)
Parent gene strand	-
Alternative splicing	Os03g0197000_circ_g.2 Os03g0197000_circ_g.3 Os03g0197000_circ_g.4 Os03g0197000_circ_g.5
Support reads	2/1
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0197000-01:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AGATCAGATCAAACCATAAACTGCAAAGGCTGATTGCTAAGTGGACGATGAACCAAAGCAACCC
CAGAATCAGCTTGATAATCACTGATGGTCGAGACATctattgctttcaatgcaccggacatgtc
agcttctgtaaatactatttcaccatttccacaagtattcctattgccgttcacacaagttgag
caaagtaa

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 562
Transcript exons: 5087401-5087605,5087696-5087763,5088075-5088363
CTATTGCTTTCAATGCACCGGACATGTCAGCTTCTGTAAATACTATTTCACCATTTCCACAAGT
ATTCCTATTGCCGTTCACACAAGTTGAGCAAAGTAAGCTGGCTGCTTTAGCAATCCCATCCTTG
CCATCAGAACCAGAAATACACTCTATTGTCATCTCTTTATGACAACAGGTCAAAGCTTTTGTAT
TTGCACCCACATCCAGATATTTTCCTGAATTCCCATATCTTGAACCATTCATAGCAGGTGCAAA
TGTTTTCTTCAGCACTCCATCAATGTCATAAAGACATACATACTTCACACCAACATTTGAGAGC
CAACATAAGAGCTGATTGATCTTTGCAACATTTTTAGCTTCTCTGCTATCTACCACAACAGCCA
AGTGTTTGAGCCTATCCAGTTGGAATTTCCGATACTTTGGAATCAGCCCGAATGAAATGAGATA
GCATTCTATACTGTAGATCAGATCAAACCATAAACTGCAAAGGCTGATTGCTAAGTGGACGATG
AACCAAAGCAACCCCAGAATCAGCTTGATAATCACTGATGGTCGAGACAT
						
					

						Genomic sequence
						
							CTATTGCTTTCAATGCACCGGACATGTCAGCTTCTGTAAATACTATTTCACCATTTCCACAAGT
ATTCCTATTGCCGTTCACACAAGTTGAGCAAAGTAAGCTGGCTGCTTTAGCAATCCCATCCTTG
CCATCAGAACCAGAAATACACTCTATTGTCATCTCTTTATGACAACAGGTCAAAGCTTTTGTAT
TTGCACCCACATCCTACATGTTGTCATGGACATGAGGGAATATCGAGATCAATGTCATGGAATA
AGAAAACAAAGCAACAAAAAGCTCGATTATGTGACTTACCAGATATTTTCCTGAATTCCCATAT
CTTGAACCATTCATAGCAGGTGCAAATGTTTTCTTCAGCACTCCTATGGTGCAACATAAAGTGT
TAATGTGCATCCCTTGTGCACTAGGTCACTAGACAGTGACACAGTGTTGCTTAAATATCAGAAA
CTAAAGTGTAAAAGGATTAAAAGGAAACATGGCTATAATTTGAATTATTCATCCACTGTTCTCC
AAATAACAAAGACCACTGTTTCTCAAAGCCATTTAATTTCTTATACTCATGTTCATTTTGATGA
AAGGGAAAATGTTATATGTATGTACTAGGCCACTGGAGATATTCTGCAAAGGAAACAATAGCAT
TCTACAAATGCAAATAGCAAACTCATTGTCTTACCATCAATGTCATAAAGACATACATACTTCA
CACCAACATTTGAGAGCCAACATAAGAGCTGATTGATCTTTGCAACATTTTTAGCTTCTCTGCT
ATCTACCACAACAGCCAAGTGTTTGAGCCTATCCAGTTGGAATTTCCGATACTTTGGAATCAGC
CCGAATGAAATGAGATAGCATTCTATACTGTAGATCAGATCAAACCATAAACTGCAAAGGCTGA
TTGCTAAGTGGACGATGAACCAAAGCAACCCCAGAATCAGCTTGATAATCACTGATGGTCGAGA
CAT
						
					

			
		

Sequences
Splice junction sequence	AGATCAGATCAAACCATAAACTGCAAAGGCTGATTGCTAAGTGGACGATGAACCAAAGCAACCC CAGAATCAGCTTGATAATCACTGATGGTCGAGACATctattgctttcaatgcaccggacatgtc agcttctgtaaatactatttcaccatttccacaagtattcctattgccgttcacacaagttgag caaagtaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 562 Transcript exons: 5087401-5087605,5087696-5087763,5088075-5088363 CTATTGCTTTCAATGCACCGGACATGTCAGCTTCTGTAAATACTATTTCACCATTTCCACAAGT ATTCCTATTGCCGTTCACACAAGTTGAGCAAAGTAAGCTGGCTGCTTTAGCAATCCCATCCTTG CCATCAGAACCAGAAATACACTCTATTGTCATCTCTTTATGACAACAGGTCAAAGCTTTTGTAT TTGCACCCACATCCAGATATTTTCCTGAATTCCCATATCTTGAACCATTCATAGCAGGTGCAAA TGTTTTCTTCAGCACTCCATCAATGTCATAAAGACATACATACTTCACACCAACATTTGAGAGC CAACATAAGAGCTGATTGATCTTTGCAACATTTTTAGCTTCTCTGCTATCTACCACAACAGCCA AGTGTTTGAGCCTATCCAGTTGGAATTTCCGATACTTTGGAATCAGCCCGAATGAAATGAGATA GCATTCTATACTGTAGATCAGATCAAACCATAAACTGCAAAGGCTGATTGCTAAGTGGACGATG AACCAAAGCAACCCCAGAATCAGCTTGATAATCACTGATGGTCGAGACAT
Genomic sequence	CTATTGCTTTCAATGCACCGGACATGTCAGCTTCTGTAAATACTATTTCACCATTTCCACAAGT ATTCCTATTGCCGTTCACACAAGTTGAGCAAAGTAAGCTGGCTGCTTTAGCAATCCCATCCTTG CCATCAGAACCAGAAATACACTCTATTGTCATCTCTTTATGACAACAGGTCAAAGCTTTTGTAT TTGCACCCACATCCTACATGTTGTCATGGACATGAGGGAATATCGAGATCAATGTCATGGAATA AGAAAACAAAGCAACAAAAAGCTCGATTATGTGACTTACCAGATATTTTCCTGAATTCCCATAT CTTGAACCATTCATAGCAGGTGCAAATGTTTTCTTCAGCACTCCTATGGTGCAACATAAAGTGT TAATGTGCATCCCTTGTGCACTAGGTCACTAGACAGTGACACAGTGTTGCTTAAATATCAGAAA CTAAAGTGTAAAAGGATTAAAAGGAAACATGGCTATAATTTGAATTATTCATCCACTGTTCTCC AAATAACAAAGACCACTGTTTCTCAAAGCCATTTAATTTCTTATACTCATGTTCATTTTGATGA AAGGGAAAATGTTATATGTATGTACTAGGCCACTGGAGATATTCTGCAAAGGAAACAATAGCAT TCTACAAATGCAAATAGCAAACTCATTGTCTTACCATCAATGTCATAAAGACATACATACTTCA CACCAACATTTGAGAGCCAACATAAGAGCTGATTGATCTTTGCAACATTTTTAGCTTCTCTGCT ATCTACCACAACAGCCAAGTGTTTGAGCCTATCCAGTTGGAATTTCCGATACTTTGGAATCAGC CCGAATGAAATGAGATAGCATTCTATACTGTAGATCAGATCAAACCATAAACTGCAAAGGCTGA TTGCTAAGTGGACGATGAACCAAAGCAACCCCAGAATCAGCTTGATAATCACTGATGGTCGAGA CAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.267054431

Conservation Information
Conserved circRNAs	NA
PMCS	0.267054431

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							5088352-5087567(+)
5088312-5087488(+)
5088365-5088274(-)
						
						Potential amino acid sequence
						
							MVETSIAFNAPDMSASVNTISPFPQVFLLPFTQVEQSKLAALAIPSLPSEPEIHSIVISL*(+)
MNQSNPRISLIITDGRDIYCFQCTGHVSFCKYYFTISTSIPIAVHTS*(+)
MSRPSVIIKLILGLLWFIVHLAISLCSLWFDLIYSIECYLISFGLIPKYRKFQLDRLKHLAVVV
DSREAKNVAKINQLLCWLSNVGVKYVCLYDIDGVLKKTFAPAMNGSRYGNSGKYLDVGANTKAL
TCCHKEMTIECISGSDGKDGIAKAASLLCSTCVNGNRNTCGNGEIVFTEADMSGALKAIDVSTI
SDYQADSGVALVHRPLSNQPLQFMV*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	5088352-5087567(+) 5088312-5087488(+) 5088365-5088274(-)
Potential amino acid sequence	MVETSIAFNAPDMSASVNTISPFPQVFLLPFTQVEQSKLAALAIPSLPSEPEIHSIVISL(+) MNQSNPRISLIITDGRDIYCFQCTGHVSFCKYYFTISTSIPIAVHTS(+) MSRPSVIIKLILGLLWFIVHLAISLCSLWFDLIYSIECYLISFGLIPKYRKFQLDRLKHLAVVV DSREAKNVAKINQLLCWLSNVGVKYVCLYDIDGVLKKTFAPAMNGSRYGNSGKYLDVGANTKAL TCCHKEMTIECISGSDGKDGIAKAASLLCSTCVNGNRNTCGNGEIVFTEADMSGALKAIDVSTI SDYQADSGVALVHRPLSNQPLQFMV*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os03g0197000_circ_g.6

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