Plant Circular RNA Database

Detail information of AT3G12980_circ_g.5

			
						 General Information
					
						CircRNA Name
						AT3G12980_circ_g.5
					
						ID in PlantcircBase
						ath_circ_021333
					
						Alias
						Ath_circ_FC1947
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr3: 4151608-4154273  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, PcircRNA_finder
					
						Parent gene
						
							AT3G12980						
					
						Parent gene annotation
						
							Histone acetyltransferase HAC5
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT3G12980_circ_g.4 AT3G12980_circ_g.6 						
					
						Support reads
						3
					
						Tissues
						inflorescences
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT3G12980.1:8
AT3G12980.2:8

General Information
CircRNA Name	AT3G12980_circ_g.5
ID in PlantcircBase	ath_circ_021333
Alias	Ath_circ_FC1947
Organism	Arabidpsis thaliana
Position	chr3: 4151608-4154273 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, PcircRNA_finder
Parent gene	AT3G12980
Parent gene annotation	Histone acetyltransferase HAC5
Parent gene strand	+
Alternative splicing	AT3G12980_circ_g.4 AT3G12980_circ_g.6
Support reads	3
Tissues	inflorescences
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT3G12980.1:8 AT3G12980.2:8

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GCAAGACCAGAGGTTGCATCGCTTGTAAGAAAATGTGGTCCCTCTTCCGATTGCACTCCCGAAA
CTGCAGAGATCCCCAATGCAAAGTCCCCAAATGCAGgtcccgggagccgactcgcttgtaatca
gagttgtagcatctgtcgacaaaatattagaagtaaagccacgtttccttgacattttccgtga
agataatt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 1592
Transcript exons: 4151608-4151736,4151852-4152052,4152141-4152283,4152375-
4152687,4152819-4152958,4153284-4153362,4153449-4153835,
4154074-4154273
GTCCCGGGAGCCGACTCGCTTGTAATCAGAGTTGTAGCATCTGTCGACAAAATATTAGAAGTAA
AGCCACGTTTCCTTGACATTTTCCGTGAAGATAATTATTCATCAGAATTCCCTTATAAGTCTAA
GGCCATTCTGTTGTTTCAGAAGATTGAAGGTGTTGAAGTATGCTTATTTGGCATGTATGTCCAG
GAATTTGGAACAGATTCTGCGTCTCCCAATCAGCGCCGGGTTTACCTTTCCTATCTGGACTCAG
TTAAGTACTTCAGACCTGACGTTAGAACAGTATCTGGAGAGGCCCTCCGTACGTTCGTATACCA
TGAAATTCTGATTGGTTACCTTGACTACTGTAAGAAACGTGGATTTTCAAGCTGCTATATATGG
GCATGCCCCCCTCTTAAGGGTGAAGATTATATTTTATATTGCCATCCTGAAATTCAGAAAACGC
CAAAGACTGATAAACTAAGGGAATGGTATCTAGCAATGCTAAAGAAAGCTTCCAAGGAAAAGGT
GGTCGTTGAGTGTACAAACTTCTATGATCACTTTTTCGTCCAGTCTGGTGAATGTAGAGCTAAG
GTAACAGCAGCAAGGTTACCATATTTTGATGGCGACTACTGGCCAGGTGCTGCTGAGGATTTAA
TAGATCAAATGAGCCAAGAGGAGGATGGCAAAAAGTCAAATAGAAAACTAATGCCCAAAAAGGT
CATATCGAAAAGGGCTCTAAAAGCAGTAGGTCAGTTGGACCTTTCTGTTAATGCCTCGAAGGAT
CTCCTGCTAATGCATAAACTGGGTGAGATCATCCTCCCAATGAAGGAAGATTTCATCATGGTTC
ATTTGCAACATTGTTGTAAGCATTGTTGTACTCTCATGGTATCTGGAAATCGTTGGGTGTGTAA
CCAGTGTAAGAATTTTCAGATTTGTGACAAGTGTCATGAAGTGGAAGAGAACCGTGTGGAAAAG
GAAAAGCATCCCGTCAATCAGAAGGAGAAGCATGTATTATATCCTGTTGCCATTGACAACATTC
CTACTGAAATTAAGGACAATGATGACATCCTCGAGAGCGAGTTCTTTGATACTAGGCAAGCTTT
CCTGAGTCTTTGCCAAGGTAATCACTATCAGTACGACACACTAAGACGGGCAAAACATTCTTCC
ATGATGATTCTCTATCATCTTCACAACCCAACCGTCCCTGCATTTGCAATGGCATGCGCCATCT
GCCAGCAAGAACTTGAAACTGCTCAAGGTTGGCGTTGCGAAGTTTGCCCTGACTATGATGTTTG
CAATGCTTGCTACTCAAAAGGCATCAACCATCCACATAGCATCATTAGCCGTCCATCTGCGACA
GATTCTGTTGTACAGAACACACAAACTAATCAAATTCAGAATGCCCAGTTAAGAGAGGTGCTGC
TACACGTAATGACATGCTGCACTGCACAGTGCCAGTATCCAAGGTGTCGCGTGATTAAGGGTCT
CATTAGGCATGGTCTTGTCTGCAAGACCAGAGGTTGCATCGCTTGTAAGAAAATGTGGTCCCTC
TTCCGATTGCACTCCCGAAACTGCAGAGATCCCCAATGCAAAGTCCCCAAATGCAG
						
					

						Genomic sequence
						
							GTCCCGGGAGCCGACTCGCTTGTAATCAGAGTTGTAGCATCTGTCGACAAAATATTAGAAGTAA
AGCCACGTTTCCTTGACATTTTCCGTGAAGATAATTATTCATCAGAATTCCCTTATAAGTCTAA
GGTACAGATTGTGTATGCTTGGTTCTTATTTCTTTTCCTTCTTTTTGTTTTCTAATTATGACCT
TCTGCTACATGATGCCTGTTGACTATCAACTTCCATACTCATTTTTTTTCAGGCCATTCTGTTG
TTTCAGAAGATTGAAGGTGTTGAAGTATGCTTATTTGGCATGTATGTCCAGGAATTTGGAACAG
ATTCTGCGTCTCCCAATCAGCGCCGGGTTTACCTTTCCTATCTGGACTCAGTTAAGTACTTCAG
ACCTGACGTTAGAACAGTATCTGGAGAGGCCCTCCGTACGTTCGTATACCATGAAATTCTGGTA
AGCCGCATTTCTTCTGATCCTAGTTGCTTTCAGGCACCTGTGTTGCTTCACAAGTCTTATCAAC
AATGGGCTTTTCGGATTGCAGATTGGTTACCTTGACTACTGTAAGAAACGTGGATTTTCAAGCT
GCTATATATGGGCATGCCCCCCTCTTAAGGGTGAAGATTATATTTTATATTGCCATCCTGAAAT
TCAGAAAACGCCAAAGACTGATAAACTAAGGGAATGGTATGGAATATATACTTTCCTGTAATAC
TTGTTTTTCACGTGTAAGAGAGAATATTGCATATAATGATGCATAATACTCCTTCCTTTTCAGG
TATCTAGCAATGCTAAAGAAAGCTTCCAAGGAAAAGGTGGTCGTTGAGTGTACAAACTTCTATG
ATCACTTTTTCGTCCAGTCTGGTGAATGTAGAGCTAAGGTAACAGCAGCAAGGTTACCATATTT
TGATGGCGACTACTGGCCAGGTGCTGCTGAGGATTTAATAGATCAAATGAGCCAAGAGGAGGAT
GGCAAAAAGTCAAATAGAAAACTAATGCCCAAAAAGGTCATATCGAAAAGGGCTCTAAAAGCAG
TAGGTCAGTTGGACCTTTCTGTTAATGCCTCGAAGGATCTCCTGCTAATGCATAAAGTAACTTC
TTTACCTTTAAGTTTTCCCTTTATTCGTTAACTTTGTTTCACTGATACTCTTACCTAATCTTCA
TGCCTTTGTTGAAGAAAGTGATATTATCAACCCCCTAACTTTTGATTGTTACTCTGTAGCTGGG
TGAGATCATCCTCCCAATGAAGGAAGATTTCATCATGGTTCATTTGCAACATTGTTGTAAGCAT
TGTTGTACTCTCATGGTATCTGGAAATCGTTGGGTGTGTAACCAGTGTAAGAATTTTCAGATTT
GTGACAAGTAAGTTTTACTTATCGGCTCGCTTTGTTTATTTGATACTTGTATAGTTTAATTAAA
TCAACTATATTGGGATGCGATATAAATTCAGAATTTCCCATAGACTGTTAAAATATCCAGCAAA
CAGATTTTTCTTCACTTAACTAAATAGCAGTATTCTAGTACGTTTGCGGGAGGGACTGTCTAGT
TTTCAAATGGGAGCTAGACAATGTTAGCTTTTAAGCACTTGGTGTATTCTGATTTTTTTTTTAG
GTTAGATTGACAGGTGTGCCTGTGAAGTATATATACATTTTTTGGCTGTATCTCTCAAGCTTTT
CGTTTACCACAGGTGTCATGAAGTGGAAGAGAACCGTGTGGAAAAGGAAAAGCATCCCGTCAAT
CAGAAGGAGAAGCATGTATTATATCCTGTAAGTTTCTACCGCGATAGGGCTCTAAAGTTTTTTG
TTTTCCCTCCTTGTTGGGTTGGAAAATGACAAGGTTTTGGTCTCTGCAGGTTGCCATTGACAAC
ATTCCTACTGAAATTAAGGACAATGATGACATCCTCGAGAGCGAGTTCTTTGATACTAGGCAAG
CTTTCCTGAGTCTTTGCCAAGGTAATCACTATCAGTACGACACACTAAGACGGGCAAAACATTC
TTCCATGATGATTCTCTATCATCTTCACAACCCAACCGTCCCTGCATTTGCAATGGCATGCGCC
ATCTGCCAGCAAGAACTTGAAACTGCTCAAGGTTGGCGTTGCGAAGTTTGCCCTGACTATGATG
TTTGCAATGCTTGCTACTCAAAAGGCATCAACCATCCACATAGCATCATTAGCCGTCCATCTGC
GACAGATTCTGTTGTACAGAACACACAAACTAATCAAATTCAGAATGCCCAGGTATTCCTTTTC
TTTCAATTTTTTAGCTTTGATCATAGAATTGATGTCCATATGCATACATAATTATTCACTTGTC
AAAGCTGGAAAGATAGATAGGCTCAACTCCTTTTAGGATTTTTACCAAAATTCCTTTCAAATAT
TGTGTAGCTAGATAAGTGTCGGTTAGAAAATTGAGTTTGCATCTCTTGGCTTCTCTTTTCCTTA
TTGCTAACTACGGTTTGGCTTGTTGGTACTGCAGTTAAGAGAGGTGCTGCTACACGTAATGACA
TGCTGCACTGCACAGTGCCAGTATCCAAGGTGTCGCGTGATTAAGGGTCTCATTAGGCATGGTC
TTGTCTGCAAGACCAGAGGTTGCATCGCTTGTAAGAAAATGTGGTCCCTCTTCCGATTGCACTC
CCGAAACTGCAGAGATCCCCAATGCAAAGTCCCCAAATGCAG
						
					

			
		

Sequences
Splice junction sequence	GCAAGACCAGAGGTTGCATCGCTTGTAAGAAAATGTGGTCCCTCTTCCGATTGCACTCCCGAAA CTGCAGAGATCCCCAATGCAAAGTCCCCAAATGCAGgtcccgggagccgactcgcttgtaatca gagttgtagcatctgtcgacaaaatattagaagtaaagccacgtttccttgacattttccgtga agataatt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 1592 Transcript exons: 4151608-4151736,4151852-4152052,4152141-4152283,4152375- 4152687,4152819-4152958,4153284-4153362,4153449-4153835, 4154074-4154273 GTCCCGGGAGCCGACTCGCTTGTAATCAGAGTTGTAGCATCTGTCGACAAAATATTAGAAGTAA AGCCACGTTTCCTTGACATTTTCCGTGAAGATAATTATTCATCAGAATTCCCTTATAAGTCTAA GGCCATTCTGTTGTTTCAGAAGATTGAAGGTGTTGAAGTATGCTTATTTGGCATGTATGTCCAG GAATTTGGAACAGATTCTGCGTCTCCCAATCAGCGCCGGGTTTACCTTTCCTATCTGGACTCAG TTAAGTACTTCAGACCTGACGTTAGAACAGTATCTGGAGAGGCCCTCCGTACGTTCGTATACCA TGAAATTCTGATTGGTTACCTTGACTACTGTAAGAAACGTGGATTTTCAAGCTGCTATATATGG GCATGCCCCCCTCTTAAGGGTGAAGATTATATTTTATATTGCCATCCTGAAATTCAGAAAACGC CAAAGACTGATAAACTAAGGGAATGGTATCTAGCAATGCTAAAGAAAGCTTCCAAGGAAAAGGT GGTCGTTGAGTGTACAAACTTCTATGATCACTTTTTCGTCCAGTCTGGTGAATGTAGAGCTAAG GTAACAGCAGCAAGGTTACCATATTTTGATGGCGACTACTGGCCAGGTGCTGCTGAGGATTTAA TAGATCAAATGAGCCAAGAGGAGGATGGCAAAAAGTCAAATAGAAAACTAATGCCCAAAAAGGT CATATCGAAAAGGGCTCTAAAAGCAGTAGGTCAGTTGGACCTTTCTGTTAATGCCTCGAAGGAT CTCCTGCTAATGCATAAACTGGGTGAGATCATCCTCCCAATGAAGGAAGATTTCATCATGGTTC ATTTGCAACATTGTTGTAAGCATTGTTGTACTCTCATGGTATCTGGAAATCGTTGGGTGTGTAA CCAGTGTAAGAATTTTCAGATTTGTGACAAGTGTCATGAAGTGGAAGAGAACCGTGTGGAAAAG GAAAAGCATCCCGTCAATCAGAAGGAGAAGCATGTATTATATCCTGTTGCCATTGACAACATTC CTACTGAAATTAAGGACAATGATGACATCCTCGAGAGCGAGTTCTTTGATACTAGGCAAGCTTT CCTGAGTCTTTGCCAAGGTAATCACTATCAGTACGACACACTAAGACGGGCAAAACATTCTTCC ATGATGATTCTCTATCATCTTCACAACCCAACCGTCCCTGCATTTGCAATGGCATGCGCCATCT GCCAGCAAGAACTTGAAACTGCTCAAGGTTGGCGTTGCGAAGTTTGCCCTGACTATGATGTTTG CAATGCTTGCTACTCAAAAGGCATCAACCATCCACATAGCATCATTAGCCGTCCATCTGCGACA GATTCTGTTGTACAGAACACACAAACTAATCAAATTCAGAATGCCCAGTTAAGAGAGGTGCTGC TACACGTAATGACATGCTGCACTGCACAGTGCCAGTATCCAAGGTGTCGCGTGATTAAGGGTCT CATTAGGCATGGTCTTGTCTGCAAGACCAGAGGTTGCATCGCTTGTAAGAAAATGTGGTCCCTC TTCCGATTGCACTCCCGAAACTGCAGAGATCCCCAATGCAAAGTCCCCAAATGCAG
Genomic sequence	GTCCCGGGAGCCGACTCGCTTGTAATCAGAGTTGTAGCATCTGTCGACAAAATATTAGAAGTAA AGCCACGTTTCCTTGACATTTTCCGTGAAGATAATTATTCATCAGAATTCCCTTATAAGTCTAA GGTACAGATTGTGTATGCTTGGTTCTTATTTCTTTTCCTTCTTTTTGTTTTCTAATTATGACCT TCTGCTACATGATGCCTGTTGACTATCAACTTCCATACTCATTTTTTTTCAGGCCATTCTGTTG TTTCAGAAGATTGAAGGTGTTGAAGTATGCTTATTTGGCATGTATGTCCAGGAATTTGGAACAG ATTCTGCGTCTCCCAATCAGCGCCGGGTTTACCTTTCCTATCTGGACTCAGTTAAGTACTTCAG ACCTGACGTTAGAACAGTATCTGGAGAGGCCCTCCGTACGTTCGTATACCATGAAATTCTGGTA AGCCGCATTTCTTCTGATCCTAGTTGCTTTCAGGCACCTGTGTTGCTTCACAAGTCTTATCAAC AATGGGCTTTTCGGATTGCAGATTGGTTACCTTGACTACTGTAAGAAACGTGGATTTTCAAGCT GCTATATATGGGCATGCCCCCCTCTTAAGGGTGAAGATTATATTTTATATTGCCATCCTGAAAT TCAGAAAACGCCAAAGACTGATAAACTAAGGGAATGGTATGGAATATATACTTTCCTGTAATAC TTGTTTTTCACGTGTAAGAGAGAATATTGCATATAATGATGCATAATACTCCTTCCTTTTCAGG TATCTAGCAATGCTAAAGAAAGCTTCCAAGGAAAAGGTGGTCGTTGAGTGTACAAACTTCTATG ATCACTTTTTCGTCCAGTCTGGTGAATGTAGAGCTAAGGTAACAGCAGCAAGGTTACCATATTT TGATGGCGACTACTGGCCAGGTGCTGCTGAGGATTTAATAGATCAAATGAGCCAAGAGGAGGAT GGCAAAAAGTCAAATAGAAAACTAATGCCCAAAAAGGTCATATCGAAAAGGGCTCTAAAAGCAG TAGGTCAGTTGGACCTTTCTGTTAATGCCTCGAAGGATCTCCTGCTAATGCATAAAGTAACTTC TTTACCTTTAAGTTTTCCCTTTATTCGTTAACTTTGTTTCACTGATACTCTTACCTAATCTTCA TGCCTTTGTTGAAGAAAGTGATATTATCAACCCCCTAACTTTTGATTGTTACTCTGTAGCTGGG TGAGATCATCCTCCCAATGAAGGAAGATTTCATCATGGTTCATTTGCAACATTGTTGTAAGCAT TGTTGTACTCTCATGGTATCTGGAAATCGTTGGGTGTGTAACCAGTGTAAGAATTTTCAGATTT GTGACAAGTAAGTTTTACTTATCGGCTCGCTTTGTTTATTTGATACTTGTATAGTTTAATTAAA TCAACTATATTGGGATGCGATATAAATTCAGAATTTCCCATAGACTGTTAAAATATCCAGCAAA CAGATTTTTCTTCACTTAACTAAATAGCAGTATTCTAGTACGTTTGCGGGAGGGACTGTCTAGT TTTCAAATGGGAGCTAGACAATGTTAGCTTTTAAGCACTTGGTGTATTCTGATTTTTTTTTTAG GTTAGATTGACAGGTGTGCCTGTGAAGTATATATACATTTTTTGGCTGTATCTCTCAAGCTTTT CGTTTACCACAGGTGTCATGAAGTGGAAGAGAACCGTGTGGAAAAGGAAAAGCATCCCGTCAAT CAGAAGGAGAAGCATGTATTATATCCTGTAAGTTTCTACCGCGATAGGGCTCTAAAGTTTTTTG TTTTCCCTCCTTGTTGGGTTGGAAAATGACAAGGTTTTGGTCTCTGCAGGTTGCCATTGACAAC ATTCCTACTGAAATTAAGGACAATGATGACATCCTCGAGAGCGAGTTCTTTGATACTAGGCAAG CTTTCCTGAGTCTTTGCCAAGGTAATCACTATCAGTACGACACACTAAGACGGGCAAAACATTC TTCCATGATGATTCTCTATCATCTTCACAACCCAACCGTCCCTGCATTTGCAATGGCATGCGCC ATCTGCCAGCAAGAACTTGAAACTGCTCAAGGTTGGCGTTGCGAAGTTTGCCCTGACTATGATG TTTGCAATGCTTGCTACTCAAAAGGCATCAACCATCCACATAGCATCATTAGCCGTCCATCTGC GACAGATTCTGTTGTACAGAACACACAAACTAATCAAATTCAGAATGCCCAGGTATTCCTTTTC TTTCAATTTTTTAGCTTTGATCATAGAATTGATGTCCATATGCATACATAATTATTCACTTGTC AAAGCTGGAAAGATAGATAGGCTCAACTCCTTTTAGGATTTTTACCAAAATTCCTTTCAAATAT TGTGTAGCTAGATAAGTGTCGGTTAGAAAATTGAGTTTGCATCTCTTGGCTTCTCTTTTCCTTA TTGCTAACTACGGTTTGGCTTGTTGGTACTGCAGTTAAGAGAGGTGCTGCTACACGTAATGACA TGCTGCACTGCACAGTGCCAGTATCCAAGGTGTCGCGTGATTAAGGGTCTCATTAGGCATGGTC TTGTCTGCAAGACCAGAGGTTGCATCGCTTGTAAGAAAATGTGGTCCCTCTTCCGATTGCACTC CCGAAACTGCAGAGATCCCCAATGCAAAGTCCCCAAATGCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.219804628

Conservation Information
Conserved circRNAs	NA
PMCS	0.219804628

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017;Chen et al., 2017a

circRNA

Detail information of AT3G12980_circ_g.5

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