Plant Circular RNA Database

Detail information of AT4G23270_circ_g.4

			
						 General Information
					
						CircRNA Name
						AT4G23270_circ_g.4
					
						ID in PlantcircBase
						ath_circ_032301
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr4: 12172341-12172600  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							i-circRNA							
									
						Identification method
						CIRCexplorer, circseq_cup
					
						Parent gene
						
							AT4G23270						
					
						Parent gene annotation
						
							cysteine-rich RLK (RECEPTOR-like protein kinase) 19
							
						Parent gene strand
						+
					
						Alternative splicing
						
							4_circ_ag.2 4_circ_ag.3 4_circ_ag.4 AT4G23180_circ_g.1 AT4G23180_circ_g.2 AT4G23190_circ_g.1 AT4G23190_circ_g.2 AT4G23190_circ_g.3 4_circ_ag.4 AT4G23220_circ_g.1 4_circ_ag.2 4_circ_ag.3 AT4G23250_circ_g.1 4_circ_ag.2 AT4G23250_circ_g.3 4_circ_ag.4 AT4G23270_circ_g.1 AT4G23270_circ_g.2 AT4G23270_circ_g.3 4_circ_ag.5 4_circ_ag.6 4_circ_ag.7 4_circ_ag.8 AT4G23300_circ_g.1 AT4G23300_circ_g.2 AT4G23320_circ_g.1 						
					
						Support reads
						8
					
						Tissues
						seedlings
					
						Exon boundary  
						
							No-No							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							TG-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						0

General Information
CircRNA Name	AT4G23270_circ_g.4
ID in PlantcircBase	ath_circ_032301
Alias	NA
Organism	Arabidpsis thaliana
Position	chr4: 12172341-12172600 JBrowse»
Reference genome	TAIR10.38
Type	i-circRNA
Identification method	CIRCexplorer, circseq_cup
Parent gene	AT4G23270
Parent gene annotation	cysteine-rich RLK (RECEPTOR-like protein kinase) 19
Parent gene strand	+
Alternative splicing	4_circ_ag.2 4_circ_ag.3 4_circ_ag.4 AT4G23180_circ_g.1 AT4G23180_circ_g.2 AT4G23190_circ_g.1 AT4G23190_circ_g.2 AT4G23190_circ_g.3 4_circ_ag.4 AT4G23220_circ_g.1 4_circ_ag.2 4_circ_ag.3 AT4G23250_circ_g.1 4_circ_ag.2 AT4G23250_circ_g.3 4_circ_ag.4 AT4G23270_circ_g.1 AT4G23270_circ_g.2 AT4G23270_circ_g.3 4_circ_ag.5 4_circ_ag.6 4_circ_ag.7 4_circ_ag.8 AT4G23300_circ_g.1 AT4G23300_circ_g.2 AT4G23320_circ_g.1
Support reads	8
Tissues	seedlings
Exon boundary	No-No "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	TG-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	0

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCATCCTATATTTGTAACAAATACTAACTTCTACTTGTATACTTGCATTTTGAGTACTTCATTT
TCATGATTAATCTTACCGACAATTCCTATATACTTAgtattattaccattgaacagagcaatgt
actctgtttttcccctgtttccaaatcaaatctcacacacattacttcgaaagttgagatgtat
gggatgag

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 260
Transcript exons: 12172341-12172600
GTATTATTACCATTGAACAGAGCAATGTACTCTGTTTTTCCCCTGTTTCCAAATCAAATCTCAC
ACACATTACTTCGAAAGTTGAGATGTATGGGATGAGTGCAGCAACCTTGTACTTGCAAAGGTTG
TTGCGGAACAAAGAAACTCTAGTCGTTAATTATCATCCTATATTTGTAACAAATACTAACTTCT
ACTTGTATACTTGCATTTTGAGTACTTCATTTTCATGATTAATCTTACCGACAATTCCTATATA
CTTA
Transcript length: 260
Transcript exons: 12172341-12172600
GTATTATTACCATTGAACAGAGCAATGTACTCTGTTTTTCCCCTGTTTCCAAATCAAATCTCAC
ACACATTACTTCGAAAGTTGAGATGTATGGGATGAGTGCAGCAACCTTGTACTTGCAAAGGTTG
TTGCGGAACAAAGAAACTCTAGTCGTTAATTATCATCCTATATTTGTAACAAATACTAACTTCT
ACTTGTATACTTGCATTTTGAGTACTTCATTTTCATGATTAATCTTACCGACAATTCCTATATA
CTTG
						
						Genomic sequence
						
							GTATTATTACCATTGAACAGAGCAATGTACTCTGTTTTTCCCCTGTTTCCAAATCAAATCTCAC
ACACATTACTTCGAAAGTTGAGATGTATGGGATGAGTGCAGCAACCTTGTACTTGCAAAGGTTG
TTGCGGAACAAAGAAACTCTAGTCGTTAATTATCATCCTATATTTGTAACAAATACTAACTTCT
ACTTGTATACTTGCATTTTGAGTACTTCATTTTCATGATTAATCTTACCGACAATTCCTATATA
CTTA

Sequences
Splice junction sequence	TCATCCTATATTTGTAACAAATACTAACTTCTACTTGTATACTTGCATTTTGAGTACTTCATTT TCATGATTAATCTTACCGACAATTCCTATATACTTAgtattattaccattgaacagagcaatgt actctgtttttcccctgtttccaaatcaaatctcacacacattacttcgaaagttgagatgtat gggatgag
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 260 Transcript exons: 12172341-12172600 GTATTATTACCATTGAACAGAGCAATGTACTCTGTTTTTCCCCTGTTTCCAAATCAAATCTCAC ACACATTACTTCGAAAGTTGAGATGTATGGGATGAGTGCAGCAACCTTGTACTTGCAAAGGTTG TTGCGGAACAAAGAAACTCTAGTCGTTAATTATCATCCTATATTTGTAACAAATACTAACTTCT ACTTGTATACTTGCATTTTGAGTACTTCATTTTCATGATTAATCTTACCGACAATTCCTATATA CTTA Transcript length: 260 Transcript exons: 12172341-12172600 GTATTATTACCATTGAACAGAGCAATGTACTCTGTTTTTCCCCTGTTTCCAAATCAAATCTCAC ACACATTACTTCGAAAGTTGAGATGTATGGGATGAGTGCAGCAACCTTGTACTTGCAAAGGTTG TTGCGGAACAAAGAAACTCTAGTCGTTAATTATCATCCTATATTTGTAACAAATACTAACTTCT ACTTGTATACTTGCATTTTGAGTACTTCATTTTCATGATTAATCTTACCGACAATTCCTATATA CTTG
Genomic sequence	GTATTATTACCATTGAACAGAGCAATGTACTCTGTTTTTCCCCTGTTTCCAAATCAAATCTCAC ACACATTACTTCGAAAGTTGAGATGTATGGGATGAGTGCAGCAACCTTGTACTTGCAAAGGTTG TTGCGGAACAAAGAAACTCTAGTCGTTAATTATCATCCTATATTTGTAACAAATACTAACTTCT ACTTGTATACTTGCATTTTGAGTACTTCATTTTCATGATTAATCTTACCGACAATTCCTATATA CTTA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.544667221

Conservation Information
Conserved circRNAs	NA
PMCS	0.544667221

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to heat stress

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to heat stress

			
						 Other Information
					
						References
						Pan et al., 2017

circRNA

Detail information of AT4G23270_circ_g.4

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