Plant Circular RNA Database

Detail information of Os10g0188300_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os10g0188300_circ_g.3
					
						ID in PlantcircBase
						osa_circ_006364
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr10: 6107274-6107641  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ie-circRNA							
									
						Identification method
						CIRCexplorer, CIRI, KNIFE, PcircRNA_finder, circseq_cup
					
						Parent gene
						
							Os10g0188275						
					
						Parent gene annotation
						
							Hypothetical protein. (Os10t0188275-00)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os10g0188300_circ_g.1 Os10g0188300_circ_g.2 						
					
						Support reads
						2/5
					
						Tissues
						root/shoot, root, seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os10t0188300-01:2
Os10t0188275-00:1
Os10t0188300-01:2

General Information
CircRNA Name	Os10g0188300_circ_g.3
ID in PlantcircBase	osa_circ_006364
Alias	NA
Organism	Oryza sativa
Position	chr10: 6107274-6107641 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ie-circRNA
Identification method	CIRCexplorer, CIRI, KNIFE, PcircRNA_finder, circseq_cup
Parent gene	Os10g0188275
Parent gene annotation	Hypothetical protein. (Os10t0188275-00)
Parent gene strand	-
Alternative splicing	Os10g0188300_circ_g.1 Os10g0188300_circ_g.2
Support reads	2/5
Tissues	root/shoot, root, seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os10t0188300-01:2 Os10t0188275-00:1 Os10t0188300-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AAATGATCATGGTCTTAATTTTGTTATTTTCTTCACACCCAGCTATGTGATAATGTGCATCCAC
TACTTACAGTCACAGAGAATACTCCCATGTCTACAGgcattaactcgggcaagggtcccaatag
tgaagcttatggatccaaatacagggctatcttgtgacatatgtgtcaataaccttttagcagt
tgttaaca

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 282
Transcript exons: 6107274-6107497,6107584-6107641
GCATTAACTCGGGCAAGGGTCCCAATAGTGAAGCTTATGGATCCAAATACAGGGCTATCTTGTG
ACATATGTGTCAATAACCTTTTAGCAGTTGTTAACACAAAACTTCTAAGAGATTATTCACGAAT
TGATAAAAGGTTACGGCCATTAGCATTCATTGTGAAGCATTGGGCTAAATCTCGGTGTGTAAAT
GAAACTTACCAAGGAACACTTTCTAGTTATGCCTATGTGATAATGTGCATCCACTACTTACAGT
CACAGAGAATACTCCCATGTCTACAG
						
						Genomic sequence
						
							GCATTAACTCGGGCAAGGGTCCCAATAGTGAAGCTTATGGATCCAAATACAGGGCTATCTTGTG
ACATATGTGTCAATAACCTTTTAGCAGTTGTTAACACAAAACTTCTAAGAGATTATTCACGAAT
TGATAAAAGGTTACGGCCATTAGCATTCATTGTGAAGCATTGGGCTAAATCTCGGTGTGTAAAT
GAAACTTACCAAGGAACACTTTCTAGTTATGCGTAAGTTTCCTGTACAACTCATTAAATGTTAT
CCCCTTATGTCTAAATGATCATGGTCTTAATTTTGTTATTTTCTTCACACCCAGCTATGTGATA
ATGTGCATCCACTACTTACAGTCACAGAGAATACTCCCATGTCTACAG

Sequences
Splice junction sequence	AAATGATCATGGTCTTAATTTTGTTATTTTCTTCACACCCAGCTATGTGATAATGTGCATCCAC TACTTACAGTCACAGAGAATACTCCCATGTCTACAGgcattaactcgggcaagggtcccaatag tgaagcttatggatccaaatacagggctatcttgtgacatatgtgtcaataaccttttagcagt tgttaaca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 282 Transcript exons: 6107274-6107497,6107584-6107641 GCATTAACTCGGGCAAGGGTCCCAATAGTGAAGCTTATGGATCCAAATACAGGGCTATCTTGTG ACATATGTGTCAATAACCTTTTAGCAGTTGTTAACACAAAACTTCTAAGAGATTATTCACGAAT TGATAAAAGGTTACGGCCATTAGCATTCATTGTGAAGCATTGGGCTAAATCTCGGTGTGTAAAT GAAACTTACCAAGGAACACTTTCTAGTTATGCCTATGTGATAATGTGCATCCACTACTTACAGT CACAGAGAATACTCCCATGTCTACAG
Genomic sequence	GCATTAACTCGGGCAAGGGTCCCAATAGTGAAGCTTATGGATCCAAATACAGGGCTATCTTGTG ACATATGTGTCAATAACCTTTTAGCAGTTGTTAACACAAAACTTCTAAGAGATTATTCACGAAT TGATAAAAGGTTACGGCCATTAGCATTCATTGTGAAGCATTGGGCTAAATCTCGGTGTGTAAAT GAAACTTACCAAGGAACACTTTCTAGTTATGCGTAAGTTTCCTGTACAACTCATTAAATGTTAT CCCCTTATGTCTAAATGATCATGGTCTTAATTTTGTTATTTTCTTCACACCCAGCTATGTGATA ATGTGCATCCACTACTTACAGTCACAGAGAATACTCCCATGTCTACAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_002765* osi_circ_008833 						
					
						PMCS
						0.599858929

Conservation Information
Conserved circRNAs	osi_circ_002765* osi_circ_008833
PMCS	0.599858929

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							6107311-6107638(+)
6107633-6107335(+)
6107343-6107607(-)
6107279-6107490(-)
						
						Potential amino acid sequence
						
							MDPNTGLSCDICVNNLLAVVNTKLLRDYSRIDKRLRPLAFIVKHWAKSRCVNETYQGTLSSYAY
VIMCIHYLQSQRILPCLQALTRARVPIVKLMDPNTGLSCDICVNNLLAVVNTKLLRDYSRIDKR
LRPLAFIVKHWAKSRCVNETYQGTLSSYAYVIMCIHYLQSQRILPCLQALTRARVPIVKLMDPN
TGLSCDICVNNLLAVVNTKLLRDYSRIDKRLRPLAFIVKHWAKSRCVNETYQGTLSSYAYVIMC
IHYLQSQRILPCLQALTRARVPIVKLMDPNTGLSCDICVNNLLAVVNTKLLRDYSRIDKRLRPL
AFIVKHWAKSRCVNETYQGTLSSYAYVIMCIHYLQSQRILPCLQ(+)
MSTGINSGKGPNSEAYGSKYRAIL*(+)
MSQDSPVFGSISFTIGTLARVNACRHGSILCDCK*(-)
MPVDMGVFSVTVSSGCTLSHRHN*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	6107311-6107638(+) 6107633-6107335(+) 6107343-6107607(-) 6107279-6107490(-)
Potential amino acid sequence	MDPNTGLSCDICVNNLLAVVNTKLLRDYSRIDKRLRPLAFIVKHWAKSRCVNETYQGTLSSYAY VIMCIHYLQSQRILPCLQALTRARVPIVKLMDPNTGLSCDICVNNLLAVVNTKLLRDYSRIDKR LRPLAFIVKHWAKSRCVNETYQGTLSSYAYVIMCIHYLQSQRILPCLQALTRARVPIVKLMDPN TGLSCDICVNNLLAVVNTKLLRDYSRIDKRLRPLAFIVKHWAKSRCVNETYQGTLSSYAYVIMC IHYLQSQRILPCLQALTRARVPIVKLMDPNTGLSCDICVNNLLAVVNTKLLRDYSRIDKRLRPL AFIVKHWAKSRCVNETYQGTLSSYAYVIMCIHYLQSQRILPCLQ(+) MSTGINSGKGPNSEAYGSKYRAIL(+) MSQDSPVFGSISFTIGTLARVNACRHGSILCDCK(-) MPVDMGVFSVTVSSGCTLSHRHN*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2016;Chu et al., 2017

circRNA

Detail information of Os10g0188300_circ_g.3

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