Plant Circular RNA Database

Detail information of AT2G42500_circ_g.6

			
						 General Information
					
						CircRNA Name
						AT2G42500_circ_g.6
					
						ID in PlantcircBase
						ath_circ_017953
					
						Alias
						At_ciR4307, AT2G42500_C1
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr2: 17699176-17700174  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, CIRI, PcircRNA_finder, find_circ, CIRI-full, CIRI2
					
						Parent gene
						
							AT2G42500						
					
						Parent gene annotation
						
							Serine/threonine-protein phosphatase PP2A-3 catalytic subunit
							
						Parent gene strand
						-
					
						Alternative splicing
						
							AT2G42500_circ_g.1 AT2G42500_circ_g.2 AT2G42500_circ_g.3 AT2G42500_circ_g.4 AT2G42500_circ_g.5 AT2G42500_circ_g.7 AT2G42500_circ_g.8 AT2G42500_circ_g.9 AT2G42500_circ_g.10 						
					
						Support reads
						3/2
					
						Tissues
						leaf/root, aerial, whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT2G42500.2:4
AT2G42500.5:4
AT2G42500.4:4
AT2G42500.3:4
AT2G42500.1:4

General Information
CircRNA Name	AT2G42500_circ_g.6
ID in PlantcircBase	ath_circ_017953
Alias	At_ciR4307, AT2G42500_C1
Organism	Arabidpsis thaliana
Position	chr2: 17699176-17700174 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, CIRI, PcircRNA_finder, find_circ, CIRI-full, CIRI2
Parent gene	AT2G42500
Parent gene annotation	Serine/threonine-protein phosphatase PP2A-3 catalytic subunit
Parent gene strand	-
Alternative splicing	AT2G42500_circ_g.1 AT2G42500_circ_g.2 AT2G42500_circ_g.3 AT2G42500_circ_g.4 AT2G42500_circ_g.5 AT2G42500_circ_g.7 AT2G42500_circ_g.8 AT2G42500_circ_g.9 AT2G42500_circ_g.10
Support reads	3/2
Tissues	leaf/root, aerial, whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT2G42500.2:4 AT2G42500.5:4 AT2G42500.4:4 AT2G42500.3:4 AT2G42500.1:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AGGAAGTCTAAATATTTCATCTTCGTACCGTAACAGTTTCAACAGAATAATAACCACGGTCCAC
ATAGTCTCCCATAAACAGGTAATTGGTATCAGGGCAcaaggctgtcagaggaaaatagtcgaag
aggtctgtaaagattttccaaacatttgcgttgccgtacctaaaaaggaaaacatggtttgtta
aatttatt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 252
Transcript exons: 17699176-17699242,17699554-17699594,17699835-17699906,17
700103-17700174
CAAGGCTGTCAGAGGAAAATAGTCGAAGAGGTCTGTAAAGATTTTCCAAACATTTGCGTTGCCG
TACTTTCGTAGACATTCATCATAAAATCCATAAACCTGAGTAATCTGACGACTTTCATGGTTTC
CTCTAAGAATAGTGATTCGCTGAGGATATCGCATCTTTAAGGCGACTAACAGCGTAACAGTTTC
AACAGAATAATAACCACGGTCCACATAGTCTCCCATAAACAGGTAATTGGTATCAGGGCA
Transcript length: 211
Transcript exons: 17699176-17700174
CAAGGCTGTCAGAGGAAAATAGTCGAAGAGGTCTGTAAAGATTTTCCAAACATTTGCGTTGCCG
TACCTGACGACTTTCATGGTTTCCTCTAAGAATAGTGATTCGCTGAGGATATCGCATCTTTAAG
GCGACTAACAGCGTAACAGTTTCAACAGAATAATAACCACGGTCCACATAGTCTCCCATAAACA
GGTAATTGGTATCAGGGCA
						
					

						Genomic sequence
						
							CAAGGCTGTCAGAGGAAAATAGTCGAAGAGGTCTGTAAAGATTTTCCAAACATTTGCGTTGCCG
TACCTAAAAAGGAAAACATGGTTTGTTAAATTTATTGCAAAATCTATATCCCAAATAAGCTGCA
AACTAGGATGTGTAAGATTATACAACATAGAACAATACTATAAGCAAGTAATCATAAGCCCAAA
GGATTAACAGAAACAGGAAGTAAAGAAAGAAAAGACAATTTTGGACAGGCTACCATACACACAT
ACAATGCAACAGCAAAAATATAAGGACAATAGTAATGCTAATAATAATTAAAAAAATTGTTTAA
ATGCATTAGAAAATAAGGATTTTTTTTCCAGTATAAGGATTTTTTCCAGGAGACTCACTTTCGT
AGACATTCATCATAAAATCCATAAACCTGAGTAATCTGCGAGATGGAAGAAAAAGAAGAAAAGC
CTGTGTCAGATTAAAATAGTAATATGATAAAAAAAATGTGCACAAAACGAATTTTGTAGACAAA
ACAGAGACTTATAAAAAAGGAAATTGTGTGTCAACCAAGATCTCAACGATGGAAAAGACTGGAT
ACACAATGAAGTACTGTATTTAACATCAGAAGGTGAATTTACAAGTGGAACCAGAGTAATAAAA
TCATAAAGCATTAGTCTACCTGACGACTTTCATGGTTTCCTCTAAGAATAGTGATTCGCTGAGG
ATATCGCATCTTTAAGGCGACTAACAGCTGGAAAGAGAATAAGAAAGAGAAACAGTATCAGCAT
ACTTCATAGTCACACCCAAAATATATACATTGTAAAGAAAAAAATATCCGTCAAGTTAATGGTA
TCTTGTTACCAATATAAAAAGACAATGACTGGCTTGCCAATCAGCCTACTCTTAAATGAATAAA
AAAAGGAAGTCTAAATATTTCATCTTCGTACCGTAACAGTTTCAACAGAATAATAACCACGGTC
CACATAGTCTCCCATAAACAGGTAATTGGTATCAGGGCA
						
					

			
		

Sequences
Splice junction sequence	AGGAAGTCTAAATATTTCATCTTCGTACCGTAACAGTTTCAACAGAATAATAACCACGGTCCAC ATAGTCTCCCATAAACAGGTAATTGGTATCAGGGCAcaaggctgtcagaggaaaatagtcgaag aggtctgtaaagattttccaaacatttgcgttgccgtacctaaaaaggaaaacatggtttgtta aatttatt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 252 Transcript exons: 17699176-17699242,17699554-17699594,17699835-17699906,17 700103-17700174 CAAGGCTGTCAGAGGAAAATAGTCGAAGAGGTCTGTAAAGATTTTCCAAACATTTGCGTTGCCG TACTTTCGTAGACATTCATCATAAAATCCATAAACCTGAGTAATCTGACGACTTTCATGGTTTC CTCTAAGAATAGTGATTCGCTGAGGATATCGCATCTTTAAGGCGACTAACAGCGTAACAGTTTC AACAGAATAATAACCACGGTCCACATAGTCTCCCATAAACAGGTAATTGGTATCAGGGCA Transcript length: 211 Transcript exons: 17699176-17700174 CAAGGCTGTCAGAGGAAAATAGTCGAAGAGGTCTGTAAAGATTTTCCAAACATTTGCGTTGCCG TACCTGACGACTTTCATGGTTTCCTCTAAGAATAGTGATTCGCTGAGGATATCGCATCTTTAAG GCGACTAACAGCGTAACAGTTTCAACAGAATAATAACCACGGTCCACATAGTCTCCCATAAACA GGTAATTGGTATCAGGGCA
Genomic sequence	CAAGGCTGTCAGAGGAAAATAGTCGAAGAGGTCTGTAAAGATTTTCCAAACATTTGCGTTGCCG TACCTAAAAAGGAAAACATGGTTTGTTAAATTTATTGCAAAATCTATATCCCAAATAAGCTGCA AACTAGGATGTGTAAGATTATACAACATAGAACAATACTATAAGCAAGTAATCATAAGCCCAAA GGATTAACAGAAACAGGAAGTAAAGAAAGAAAAGACAATTTTGGACAGGCTACCATACACACAT ACAATGCAACAGCAAAAATATAAGGACAATAGTAATGCTAATAATAATTAAAAAAATTGTTTAA ATGCATTAGAAAATAAGGATTTTTTTTCCAGTATAAGGATTTTTTCCAGGAGACTCACTTTCGT AGACATTCATCATAAAATCCATAAACCTGAGTAATCTGCGAGATGGAAGAAAAAGAAGAAAAGC CTGTGTCAGATTAAAATAGTAATATGATAAAAAAAATGTGCACAAAACGAATTTTGTAGACAAA ACAGAGACTTATAAAAAAGGAAATTGTGTGTCAACCAAGATCTCAACGATGGAAAAGACTGGAT ACACAATGAAGTACTGTATTTAACATCAGAAGGTGAATTTACAAGTGGAACCAGAGTAATAAAA TCATAAAGCATTAGTCTACCTGACGACTTTCATGGTTTCCTCTAAGAATAGTGATTCGCTGAGG ATATCGCATCTTTAAGGCGACTAACAGCTGGAAAGAGAATAAGAAAGAGAAACAGTATCAGCAT ACTTCATAGTCACACCCAAAATATATACATTGTAAAGAAAAAAATATCCGTCAAGTTAATGGTA TCTTGTTACCAATATAAAAAGACAATGACTGGCTTGCCAATCAGCCTACTCTTAAATGAATAAA AAAAGGAAGTCTAAATATTTCATCTTCGTACCGTAACAGTTTCAACAGAATAATAACCACGGTC CACATAGTCTCCCATAAACAGGTAATTGGTATCAGGGCA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.223467836

Conservation Information
Conserved circRNAs	NA
PMCS	0.223467836

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							17700151-17699178(-)
						
						Potential amino acid sequence
						
							MGDYVDRGYYSVETVTLLVALKMRYPQRITILRGNHESRQITQVYGFYDECLRKYGNANVWKIF
TDLFDYFPLTALCPDTNYLFMGDYVDRGYYSVETVTLLVALKMRYPQRITILRGNHESRQITQV
YGFYDECLRKYGNANVWKIFTDLFDYFPLTALCPDTNYLFMGDYVDRGYYSVETVTLLVALKMR
YPQRITILRGNHESRQITQVYGFYDECLRKYGNANVWKIFTDLFDYFPLTALCPDTNYLFMGDY
VDRGYYSVETVTLLVALKMRYPQRITILRGNHESRQITQVYGFYDECLRKYGNANVWKIFTDLF
DYFPLTAL(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						response to drought stress

Functional Information
Coding potential	Y
Potential coding position	17700151-17699178(-)
Potential amino acid sequence	MGDYVDRGYYSVETVTLLVALKMRYPQRITILRGNHESRQITQVYGFYDECLRKYGNANVWKIF TDLFDYFPLTALCPDTNYLFMGDYVDRGYYSVETVTLLVALKMRYPQRITILRGNHESRQITQV YGFYDECLRKYGNANVWKIFTDLFDYFPLTALCPDTNYLFMGDYVDRGYYSVETVTLLVALKMR YPQRITILRGNHESRQITQVYGFYDECLRKYGNANVWKIFTDLFDYFPLTALCPDTNYLFMGDY VDRGYYSVETVTLLVALKMRYPQRITILRGNHESRQITQVYGFYDECLRKYGNANVWKIFTDLF DYFPLTAL(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	response to drought stress

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017;Zhang et al., 2019

circRNA

Detail information of AT2G42500_circ_g.6

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