Plant Circular RNA Database

Detail information of Os12g0193100_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os12g0193100_circ_g.2
					
						ID in PlantcircBase
						osa_circ_010755
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr12: 4757843-4758720  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder
					
						Parent gene
						
							Os12g0193100						
					
						Parent gene annotation
						
							Similar to cDNA clone:J013157P20, full insert sequence. (Os12t01
93100-01);Similar to cDNA clone:J013157P20, full insert sequence
. (Os12t0193100-02);Similar to cDNA clone:J013157P20, full inser
t sequence. (Os12t0193100-03);Similar to cDNA clone:J013157P20, 
full insert sequence. (Os12t0193100-04)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os12g0193100_circ_g.1 						
					
						Support reads
						5
					
						Tissues
						shoot, root, seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os12t0193100-02:2
Os12t0193100-01:2
Os12t0193100-03:2
Os12t0193100-04:2

General Information
CircRNA Name	Os12g0193100_circ_g.2
ID in PlantcircBase	osa_circ_010755
Alias	NA
Organism	Oryza sativa
Position	chr12: 4757843-4758720 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer, PcircRNA_finder
Parent gene	Os12g0193100
Parent gene annotation	Similar to cDNA clone:J013157P20, full insert sequence. (Os12t01 93100-01);Similar to cDNA clone:J013157P20, full insert sequence . (Os12t0193100-02);Similar to cDNA clone:J013157P20, full inser t sequence. (Os12t0193100-03);Similar to cDNA clone:J013157P20, full insert sequence. (Os12t0193100-04)
Parent gene strand	-
Alternative splicing	Os12g0193100_circ_g.1
Support reads	5
Tissues	shoot, root, seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os12t0193100-02:2 Os12t0193100-01:2 Os12t0193100-03:2 Os12t0193100-04:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TAGCCCCTACGTTCATCAACTACACGGATGTCAGGGTAGCCCCTGCCATCCTCTATAAAGCGAC
GTGGCAGACGATCTCTTCCTGACATGATCCCTAGTCctggggcccatggtctcccttcggaatt
agctaattctgctcgcatttgttcaatttcttgtgccatcgaaatcatattcttctccattgct
ttcttctg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 213
Transcript exons: 4757843-4757869,4758535-4758720
CTGGGGCCCATGGTCTCCCTTCGGAATCATGGATGTCAGGATAGCCCCTATGATCCTCAATGGC
ACGAATACCATGGTAGCCCCTACGATCCTCAACTACACGGATGTCAGGATAGCCCCTACGTTCA
TCAACTACACGGATGTCAGGGTAGCCCCTGCCATCCTCTATAAAGCGACGTGGCAGACGATCTC
TTCCTGACATGATCCCTAGTC
Transcript length: 789
Transcript exons: 4757843-4757988,4758078-4758720
CTGGGGCCCATGGTCTCCCTTCGGAATTAGCTAATTCTGCTCGCATTTGTTCAATTTCTTGTGC
CATCGAAATCATATTCTTCTCCATTGCTTTCTTCTGTTCAACCAACTGAAAATTTCCTTTCTGC
TCTAATTCACATGCAGACCTTGCATGAACAAGTTCCTGTCGAAGGGCATCAATCTCTGCTTTCA
ACATAGGAATCTGCTGTTTATTAGATTGTTCCCTGGTTAGTTCATGCATGATAGATGCAGACTT
CGCAGAGAGCTCTTTCCTGACTACAAGGTGCTTATCAATTTCACCCCGAAGCTGCACAATCTCA
TCTCTTAAAGACTCATTTGCTCTAAGTTCAGCTTCAAGTTTCCTCCTTTTGTCAACAAGCTCAC
CGATATAAGTTTCCTTCTTGGCACTGATGTCTGCAATGATCGTGTTCAAGTGACGGACCTCATC
TTTTCCAGCTTGTAATTCCCGGTGTAAGGCCAAACGTTCTTCAGCAAGAGCACGGTGATCTGCC
ATAAGCCTCCTGAATTCGGCCCCCTGAATCTCAATTTCTTCCTCCGGCATAGCCGTGTGAGAGC
GAGGTGCAACCCTCATGACAAGGCCTTCATGGATGTCAGGATAGCCCCTATGATCCTCAATGGC
ACGAATACCATGGTAGCCCCTACGATCCTCAACTACACGGATGTCAGGATAGCCCCTACGTTCA
TCAACTACACGGATGTCAGGGTAGCCCCTGCCATCCTCTATAAAGCGACGTGGCAGACGATCTC
TTCCTGACATGATCCCTAGTC
						
					

						Genomic sequence
						
							CTGGGGCCCATGGTCTCCCTTCGGAATTAGCTAATTCTGCTCGCATTTGTTCAATTTCTTGTGC
CATCGAAATCATATTCTTCTCCATTGCTTTCTTCTGTTCAACCAACTGAAAATTTCCTTTCTGC
TCTAATTCACATGCAGACCTGCAAAAAGTCAAATCAATCTTAAAACTTAAAGCCTCTGAATAAC
TTACAAAGGTATCTGAGAATCAGAGCTAGCAAAAGAGGCACACCTTGCATGAACAAGTTCCTGT
CGAAGGGCATCAATCTCTGCTTTCAACATAGGAATCTGCTGTTTATTAGATTGTTCCCTGGTTA
GTTCATGCATGATAGATGCAGACTTCGCAGAGAGCTCTTTCCTGACTACAAGGTGCTTATCAAT
TTCACCCCGAAGCTGCACAATCTCATCTCTTAAAGACTCATTTGCTCTAAGTTCAGCTTCAAGT
TTCCTCCTTTTGTCAACAAGCTCACCGATATAAGTTTCCTTCTTGGCACTGATGTCTGCAATGA
TCGTGTTCAAGTGACGGACCTCATCTTTTCCAGCTTGTAATTCCCGGTGTAAGGCCAAACGTTC
TTCAGCAAGAGCACGGTGATCTGCCATAAGCCTCCTGAATTCGGCCCCCTGAATCTCAATTTCT
TCCTCCGGCATAGCCGTGTGAGAGCGAGGTGCAACCCTCATGACAAGGCCTTCATGGATGTCAG
GATAGCCCCTATGATCCTCAATGGCACGAATACCATGGTAGCCCCTACGATCCTCAACTACACG
GATGTCAGGATAGCCCCTACGTTCATCAACTACACGGATGTCAGGGTAGCCCCTGCCATCCTCT
ATAAAGCGACGTGGCAGACGATCTCTTCCTGACATGATCCCTAGTC
						
					

			
		

Sequences
Splice junction sequence	TAGCCCCTACGTTCATCAACTACACGGATGTCAGGGTAGCCCCTGCCATCCTCTATAAAGCGAC GTGGCAGACGATCTCTTCCTGACATGATCCCTAGTCctggggcccatggtctcccttcggaatt agctaattctgctcgcatttgttcaatttcttgtgccatcgaaatcatattcttctccattgct ttcttctg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 213 Transcript exons: 4757843-4757869,4758535-4758720 CTGGGGCCCATGGTCTCCCTTCGGAATCATGGATGTCAGGATAGCCCCTATGATCCTCAATGGC ACGAATACCATGGTAGCCCCTACGATCCTCAACTACACGGATGTCAGGATAGCCCCTACGTTCA TCAACTACACGGATGTCAGGGTAGCCCCTGCCATCCTCTATAAAGCGACGTGGCAGACGATCTC TTCCTGACATGATCCCTAGTC Transcript length: 789 Transcript exons: 4757843-4757988,4758078-4758720 CTGGGGCCCATGGTCTCCCTTCGGAATTAGCTAATTCTGCTCGCATTTGTTCAATTTCTTGTGC CATCGAAATCATATTCTTCTCCATTGCTTTCTTCTGTTCAACCAACTGAAAATTTCCTTTCTGC TCTAATTCACATGCAGACCTTGCATGAACAAGTTCCTGTCGAAGGGCATCAATCTCTGCTTTCA ACATAGGAATCTGCTGTTTATTAGATTGTTCCCTGGTTAGTTCATGCATGATAGATGCAGACTT CGCAGAGAGCTCTTTCCTGACTACAAGGTGCTTATCAATTTCACCCCGAAGCTGCACAATCTCA TCTCTTAAAGACTCATTTGCTCTAAGTTCAGCTTCAAGTTTCCTCCTTTTGTCAACAAGCTCAC CGATATAAGTTTCCTTCTTGGCACTGATGTCTGCAATGATCGTGTTCAAGTGACGGACCTCATC TTTTCCAGCTTGTAATTCCCGGTGTAAGGCCAAACGTTCTTCAGCAAGAGCACGGTGATCTGCC ATAAGCCTCCTGAATTCGGCCCCCTGAATCTCAATTTCTTCCTCCGGCATAGCCGTGTGAGAGC GAGGTGCAACCCTCATGACAAGGCCTTCATGGATGTCAGGATAGCCCCTATGATCCTCAATGGC ACGAATACCATGGTAGCCCCTACGATCCTCAACTACACGGATGTCAGGATAGCCCCTACGTTCA TCAACTACACGGATGTCAGGGTAGCCCCTGCCATCCTCTATAAAGCGACGTGGCAGACGATCTC TTCCTGACATGATCCCTAGTC
Genomic sequence	CTGGGGCCCATGGTCTCCCTTCGGAATTAGCTAATTCTGCTCGCATTTGTTCAATTTCTTGTGC CATCGAAATCATATTCTTCTCCATTGCTTTCTTCTGTTCAACCAACTGAAAATTTCCTTTCTGC TCTAATTCACATGCAGACCTGCAAAAAGTCAAATCAATCTTAAAACTTAAAGCCTCTGAATAAC TTACAAAGGTATCTGAGAATCAGAGCTAGCAAAAGAGGCACACCTTGCATGAACAAGTTCCTGT CGAAGGGCATCAATCTCTGCTTTCAACATAGGAATCTGCTGTTTATTAGATTGTTCCCTGGTTA GTTCATGCATGATAGATGCAGACTTCGCAGAGAGCTCTTTCCTGACTACAAGGTGCTTATCAAT TTCACCCCGAAGCTGCACAATCTCATCTCTTAAAGACTCATTTGCTCTAAGTTCAGCTTCAAGT TTCCTCCTTTTGTCAACAAGCTCACCGATATAAGTTTCCTTCTTGGCACTGATGTCTGCAATGA TCGTGTTCAAGTGACGGACCTCATCTTTTCCAGCTTGTAATTCCCGGTGTAAGGCCAAACGTTC TTCAGCAAGAGCACGGTGATCTGCCATAAGCCTCCTGAATTCGGCCCCCTGAATCTCAATTTCT TCCTCCGGCATAGCCGTGTGAGAGCGAGGTGCAACCCTCATGACAAGGCCTTCATGGATGTCAG GATAGCCCCTATGATCCTCAATGGCACGAATACCATGGTAGCCCCTACGATCCTCAACTACACG GATGTCAGGATAGCCCCTACGTTCATCAACTACACGGATGTCAGGGTAGCCCCTGCCATCCTCT ATAAAGCGACGTGGCAGACGATCTCTTCCTGACATGATCCCTAGTC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.350839355

Conservation Information
Conserved circRNAs	NA
PMCS	0.350839355

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							4758709-4757952(+)
4757855-4758653(-)
4758711-4757845(-)
						
						Potential amino acid sequence
						
							MIPSPGAHGLPSELANSARICSISCAIEIIFFSIAFFCSTN*(+)
MGPRTRDHVRKRSSATSLYRGWQGLP*(-)
MSGRDRLPRRFIEDGRGYPDIRVVDERRGYPDIRVVEDRRGYHGIRAIEDHRGYPDIHEGLVMR
VAPRSHTAMPEEEIEIQGAEFRRLMADHRALAEERLALHRELQAGKDEVRHLNTIIADISAKKE
TYIGELVDKRRKLEAELRANESLRDEIVQLRGEIDKHLVVRKELSAKSASIMHELTREQSNKQQ
IPMLKAEIDALRQELVHARSACELEQKGNFQLVEQKKAMEKNMISMAQEIEQMRAELANSEGRP
WAPGLGIMSGRDRLPRRFIEDGRGYPDIRVVDERRGYPDIRVVEDRRGYHGIRAIEDHRGYPDI
HEGLVMRVAPRSHTAMPEEEIEIQGAEFRRLMADHRALAEERLALHRELQAGKDEVRHLNTIIA
DISAKKETYIGELVDKRRKLEAELRANESLRDEIVQLRGEIDKHLVVRKELSAKSASIMHELTR
EQSNKQQIPMLKAEIDALRQELVHARSACELEQKGNFQLVEQKKAMEKNMISMAQEIEQMRAEL
ANSEGRPWAPGLGIMSGRDRLPRRFIEDGRGYPDIRVVDERRGYPDIRVVEDRRGYHGIRAIED
HRGYPDIHEGLVMRVAPRSHTAMPEEEIEIQGAEFRRLMADHRALAEERLALHRELQAGKDEVR
HLNTIIADISAKKETYIGELVDKRRKLEAELRANESLRDEIVQLRGEIDKHLVVRKELSAKSAS
IMHELTREQSNKQQIPMLKAEIDALRQELVHARSACELEQKGNFQLVEQKKAMEKNMISMAQEI
EQMRAELANSEGRPWAPGLGIMSGRDRLPRRFIEDGRGYPDIRVVDERRGYPDIRVVEDRRGYH
GIRAIEDHRGYPDIHEGLVMRVAPRSHTAMPEEEIEIQGAEFRRLMADHRALAEERLALHRELQ
AGKDEVRHLNTIIADISAKKETYIGELVDKRRKLEAELRANESLRDEIVQLRGEIDKHLVVRKE
LSAKSASIMHELTREQSNKQQIPMLKAEIDALRQELVHARSACELEQKGNFQLVEQKKAMEKNM
ISMAQEIEQMRAELANSEGRPWAP(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	4758709-4757952(+) 4757855-4758653(-) 4758711-4757845(-)
Potential amino acid sequence	MIPSPGAHGLPSELANSARICSISCAIEIIFFSIAFFCSTN(+) MGPRTRDHVRKRSSATSLYRGWQGLP(-) MSGRDRLPRRFIEDGRGYPDIRVVDERRGYPDIRVVEDRRGYHGIRAIEDHRGYPDIHEGLVMR VAPRSHTAMPEEEIEIQGAEFRRLMADHRALAEERLALHRELQAGKDEVRHLNTIIADISAKKE TYIGELVDKRRKLEAELRANESLRDEIVQLRGEIDKHLVVRKELSAKSASIMHELTREQSNKQQ IPMLKAEIDALRQELVHARSACELEQKGNFQLVEQKKAMEKNMISMAQEIEQMRAELANSEGRP WAPGLGIMSGRDRLPRRFIEDGRGYPDIRVVDERRGYPDIRVVEDRRGYHGIRAIEDHRGYPDI HEGLVMRVAPRSHTAMPEEEIEIQGAEFRRLMADHRALAEERLALHRELQAGKDEVRHLNTIIA DISAKKETYIGELVDKRRKLEAELRANESLRDEIVQLRGEIDKHLVVRKELSAKSASIMHELTR EQSNKQQIPMLKAEIDALRQELVHARSACELEQKGNFQLVEQKKAMEKNMISMAQEIEQMRAEL ANSEGRPWAPGLGIMSGRDRLPRRFIEDGRGYPDIRVVDERRGYPDIRVVEDRRGYHGIRAIED HRGYPDIHEGLVMRVAPRSHTAMPEEEIEIQGAEFRRLMADHRALAEERLALHRELQAGKDEVR HLNTIIADISAKKETYIGELVDKRRKLEAELRANESLRDEIVQLRGEIDKHLVVRKELSAKSAS IMHELTREQSNKQQIPMLKAEIDALRQELVHARSACELEQKGNFQLVEQKKAMEKNMISMAQEI EQMRAELANSEGRPWAPGLGIMSGRDRLPRRFIEDGRGYPDIRVVDERRGYPDIRVVEDRRGYH GIRAIEDHRGYPDIHEGLVMRVAPRSHTAMPEEEIEIQGAEFRRLMADHRALAEERLALHRELQ AGKDEVRHLNTIIADISAKKETYIGELVDKRRKLEAELRANESLRDEIVQLRGEIDKHLVVRKE LSAKSASIMHELTREQSNKQQIPMLKAEIDALRQELVHARSACELEQKGNFQLVEQKKAMEKNM ISMAQEIEQMRAELANSEGRPWAP(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os12g0193100_circ_g.2

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