Plant Circular RNA Database

Detail information of Os05g0164100_circ_g.5

			
						 General Information
					
						CircRNA Name
						Os05g0164100_circ_g.5
					
						ID in PlantcircBase
						osa_circ_026780
					
						Alias
						Os05circ03983
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 3768053-3768717  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						SMALT, Segemehl
					
						Parent gene
						
							Os05g0164100						
					
						Parent gene annotation
						
							Similar to Fructose-6-phosphate 2-kinase/fructose-2,6-bisphospha
tase (EC 2.7.1.105) (EC 3.1.3.46) (Fragment). (Os05t0164100-01);
Similar to Fructose-6-phosphate-2-kinase/fructose-2, 6-bisphosph
atase. (Os05t0164100-02);Similar to Fructose-6-phosphate-2-kinas
e/fructose-2, 6-bisphosphatase. (Os05t0164100-03)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os05g0164100_circ_g.1 Os05g0164100_circ_g.2 Os05g0164100_circ_g.3 Os05g0164100_circ_g.4 Os05g0164100_circ_g.6 						
					
						Support reads
						8
					
						Tissues
						leaf and panicle
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0164100-03:4
Os05t0164100-02:4
Os05t0164100-01:4

General Information
CircRNA Name	Os05g0164100_circ_g.5
ID in PlantcircBase	osa_circ_026780
Alias	Os05circ03983
Organism	Oryza sativa
Position	chr5: 3768053-3768717 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	SMALT, Segemehl
Parent gene	Os05g0164100
Parent gene annotation	Similar to Fructose-6-phosphate 2-kinase/fructose-2,6-bisphospha tase (EC 2.7.1.105) (EC 3.1.3.46) (Fragment). (Os05t0164100-01); Similar to Fructose-6-phosphate-2-kinase/fructose-2, 6-bisphosph atase. (Os05t0164100-02);Similar to Fructose-6-phosphate-2-kinas e/fructose-2, 6-bisphosphatase. (Os05t0164100-03)
Parent gene strand	+
Alternative splicing	Os05g0164100_circ_g.1 Os05g0164100_circ_g.2 Os05g0164100_circ_g.3 Os05g0164100_circ_g.4 Os05g0164100_circ_g.6
Support reads	8
Tissues	leaf and panicle
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0164100-03:4 Os05t0164100-02:4 Os05t0164100-01:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: GTGTATGAGCCAGTGCAGGA
 R: TACAGTTCCCTTCAGCCATTTT
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: GTGTATGAGCCAGTGCAGGA R: TACAGTTCCCTTCAGCCATTTT PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GATTAAAACGGTTTTCCTTTTGTAGGTGTATGAGCCAGTGCAGGAAGGTTCTTACATTAAAATG
ATTGATATGGTAAAAGGGCAGGGAGGCCAGTTACAGgtcggtatatttgatgcgacaaacagca
caagaaaacgaaggtatatgctaatgaaaatggctgaagggaactgtaaggtaatttgttccat
tccgattt

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GTCGGTATATTTGATGCGACAAACAGCACAAGAAAACGAAGGTATATGCTAATGAAAATGGCTG
AAGGGAACTGTAAGGTAATTTGTTCCATTCCGATTTTGCATCAGACATGGTTCGTTTACCTTTG
CTTCATTAACTATCTCAGGCCAATCTGTGTGTCGTGGATGGAAGTGAACGATAATACTGTCACA
CTGTTGATAGTTTGTCAGTTATTTTAAGTAATGTTCTATTTGTGCCTTTAAACTTTTGTTGCTT
TCATCTTCTACAGATTATATTTCTGGAAACTATCTGTAACGATCCGAATATAATTGAAAGGAAT
GTACGTCTGAAGATACAGCAAAGTCCTGACTATGCTGACCAGTGAGGATCTCCCGAAATTTTCC
CTTTGCTAATATAAGTGAATTTGTGACCTGAATCCTGTGGAAACTTTTCAGGCCAGATTATGAA
ACTGGAGTGCGGGACTTCAAGGAACGCCTGGCAAACTATGAAAAGGTTTCGTCTAGTTATATTG
TATTGAGCCGATGTATACCAAGTAATTCTGACCTCCATGAGTCCTTTAGTATTGATTAAAACGG
TTTTCCTTTTGTAGGTGTATGAGCCAGTGCAGGAAGGTTCTTACATTAAAATGATTGATATGGT
AAAAGGGCAGGGAGGCCAGTTACAG

Sequences
Splice junction sequence	GATTAAAACGGTTTTCCTTTTGTAGGTGTATGAGCCAGTGCAGGAAGGTTCTTACATTAAAATG ATTGATATGGTAAAAGGGCAGGGAGGCCAGTTACAGgtcggtatatttgatgcgacaaacagca caagaaaacgaaggtatatgctaatgaaaatggctgaagggaactgtaaggtaatttgttccat tccgattt
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GTCGGTATATTTGATGCGACAAACAGCACAAGAAAACGAAGGTATATGCTAATGAAAATGGCTG AAGGGAACTGTAAGGTAATTTGTTCCATTCCGATTTTGCATCAGACATGGTTCGTTTACCTTTG CTTCATTAACTATCTCAGGCCAATCTGTGTGTCGTGGATGGAAGTGAACGATAATACTGTCACA CTGTTGATAGTTTGTCAGTTATTTTAAGTAATGTTCTATTTGTGCCTTTAAACTTTTGTTGCTT TCATCTTCTACAGATTATATTTCTGGAAACTATCTGTAACGATCCGAATATAATTGAAAGGAAT GTACGTCTGAAGATACAGCAAAGTCCTGACTATGCTGACCAGTGAGGATCTCCCGAAATTTTCC CTTTGCTAATATAAGTGAATTTGTGACCTGAATCCTGTGGAAACTTTTCAGGCCAGATTATGAA ACTGGAGTGCGGGACTTCAAGGAACGCCTGGCAAACTATGAAAAGGTTTCGTCTAGTTATATTG TATTGAGCCGATGTATACCAAGTAATTCTGACCTCCATGAGTCCTTTAGTATTGATTAAAACGG TTTTCCTTTTGTAGGTGTATGAGCCAGTGCAGGAAGGTTCTTACATTAAAATGATTGATATGGT AAAAGGGCAGGGAGGCCAGTTACAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.153635251

Conservation Information
Conserved circRNAs	NA
PMCS	0.153635251

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							3768099-3768714(+)
						
						Potential amino acid sequence
						
							MLMKMAEGNCKIIFLETICNDPNIIERNVRLKIQQSPDYADQPDYETGVRDFKERLANYEKVYE
PVQEGSYIKMIDMVKGQGGQLQVGIFDATNSTRKRRYMLMKMAEGNCKIIFLETICNDPNIIER
NVRLKIQQSPDYADQPDYETGVRDFKERLANYEKVYEPVQEGSYIKMIDMVKGQGGQLQVGIFD
ATNSTRKRRYMLMKMAEGNCKIIFLETICNDPNIIERNVRLKIQQSPDYADQPDYETGVRDFKE
RLANYEKVYEPVQEGSYIKMIDMVKGQGGQLQVGIFDATNSTRKRRYMLMKMAEGNCKIIFLET
ICNDPNIIERNVRLKIQQSPDYADQPDYETGVRDFKERLANYEKVYEPVQEGSYIKMIDMVKGQ
GGQLQ(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	3768099-3768714(+)
Potential amino acid sequence	MLMKMAEGNCKIIFLETICNDPNIIERNVRLKIQQSPDYADQPDYETGVRDFKERLANYEKVYE PVQEGSYIKMIDMVKGQGGQLQVGIFDATNSTRKRRYMLMKMAEGNCKIIFLETICNDPNIIER NVRLKIQQSPDYADQPDYETGVRDFKERLANYEKVYEPVQEGSYIKMIDMVKGQGGQLQVGIFD ATNSTRKRRYMLMKMAEGNCKIIFLETICNDPNIIERNVRLKIQQSPDYADQPDYETGVRDFKE RLANYEKVYEPVQEGSYIKMIDMVKGQGGQLQVGIFDATNSTRKRRYMLMKMAEGNCKIIFLET ICNDPNIIERNVRLKIQQSPDYADQPDYETGVRDFKERLANYEKVYEPVQEGSYIKMIDMVKGQ GGQLQ(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015

circRNA

Detail information of Os05g0164100_circ_g.5

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