Plant Circular RNA Database

Detail information of Os06g0133800_circ_g.5

			
						 General Information
					
						CircRNA Name
						Os06g0133800_circ_g.5
					
						ID in PlantcircBase
						osa_circ_029546
					
						Alias
						Os_ciR4903
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr6: 1810723-1811005  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os06g0133800						
					
						Parent gene annotation
						
							Similar to Transferase. (Os06t0133800-01);Similar to Transketola
se, chloroplastic. (Os06t0133800-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os06g0133800_circ_g.4 Os06g0133800_circ_g.6 						
					
						Support reads
						4/2
					
						Tissues
						root/shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os06t0133800-01:1

General Information
CircRNA Name	Os06g0133800_circ_g.5
ID in PlantcircBase	osa_circ_029546
Alias	Os_ciR4903
Organism	Oryza sativa
Position	chr6: 1810723-1811005 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os06g0133800
Parent gene annotation	Similar to Transferase. (Os06t0133800-01);Similar to Transketola se, chloroplastic. (Os06t0133800-02)
Parent gene strand	-
Alternative splicing	Os06g0133800_circ_g.4 Os06g0133800_circ_g.6
Support reads	4/2
Tissues	root/shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os06t0133800-01:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ATAGAATGCAATCAGCTTGCCAAGACCCCAATGGCCAGCCAACGAGCAAGCTTCATTGGAGATA
CCCTCCATCTGGCACCCATCTCCCAAAATACAGTAGcttgattagtgttggcttgtcagttacc
gcctttgcttccttaatggcggcacgaatctcatcatagccatcgtttccattcttaacccaga
ttgtatgc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 189
Transcript exons: 1810723-1810799,1810894-1811005
CTTGATTAGTGTTGGCTTGTCAGTTACCGCCTTTGCTTCCTTAATGGCGGCACGAATCTCATCA
TAGCCATCGTTTCGTGGTTGTCATCATAGAATGCAATCAGCTTGCCAAGACCCCAATGGCCAGC
CAACGAGCAAGCTTCATTGGAGATACCCTCCATCTGGCACCCATCTCCCAAAATACAGTAG
Transcript length: 248
Transcript exons: 1810723-1810936,1810972-1811005
CTTGATTAGTGTTGGCTTGTCAGTTACCGCCTTTGCTTCCTTAATGGCGGCACGAATCTCATCA
TAGCCATCGTTTCCATTCTTAACCCAGATTGTATGCCACCCAAGAGCCTCAAAGCGAGCACTCA
CATCCTCAGTGAATGCAATCTCTGTGTCTCCGTCAATGGAAATGTGGTTGTCATCATAGAATGC
AATCAGCTTGCCAAGACCCCAACTCCATCTGGCACCCATCTCCCAAAATACAGTAG
Transcript length: 283
Transcript exons: 1810723-1811005
CTTGATTAGTGTTGGCTTGTCAGTTACCGCCTTTGCTTCCTTAATGGCGGCACGAATCTCATCA
TAGCCATCGTTTCCATTCTTAACCCAGATTGTATGCCACCCAAGAGCCTCAAAGCGAGCACTCA
CATCCTCAGTGAATGCAATCTCTGTGTCTCCGTCAATGGAAATGTGGTTGTCATCATAGAATGC
AATCAGCTTGCCAAGACCCCAATGGCCAGCCAACGAGCAAGCTTCATTGGAGATACCCTCCATC
TGGCACCCATCTCCCAAAATACAGTAG
						
						Genomic sequence
						
							CTTGATTAGTGTTGGCTTGTCAGTTACCGCCTTTGCTTCCTTAATGGCGGCACGAATCTCATCA
TAGCCATCGTTTCCATTCTTAACCCAGATTGTATGCCACCCAAGAGCCTCAAAGCGAGCACTCA
CATCCTCAGTGAATGCAATCTCTGTGTCTCCGTCAATGGAAATGTGGTTGTCATCATAGAATGC
AATCAGCTTGCCAAGACCCCAATGGCCAGCCAACGAGCAAGCTTCATTGGAGATACCCTCCATC
TGGCACCCATCTCCCAAAATACAGTAG

Sequences
Splice junction sequence	ATAGAATGCAATCAGCTTGCCAAGACCCCAATGGCCAGCCAACGAGCAAGCTTCATTGGAGATA CCCTCCATCTGGCACCCATCTCCCAAAATACAGTAGcttgattagtgttggcttgtcagttacc gcctttgcttccttaatggcggcacgaatctcatcatagccatcgtttccattcttaacccaga ttgtatgc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 189 Transcript exons: 1810723-1810799,1810894-1811005 CTTGATTAGTGTTGGCTTGTCAGTTACCGCCTTTGCTTCCTTAATGGCGGCACGAATCTCATCA TAGCCATCGTTTCGTGGTTGTCATCATAGAATGCAATCAGCTTGCCAAGACCCCAATGGCCAGC CAACGAGCAAGCTTCATTGGAGATACCCTCCATCTGGCACCCATCTCCCAAAATACAGTAG Transcript length: 248 Transcript exons: 1810723-1810936,1810972-1811005 CTTGATTAGTGTTGGCTTGTCAGTTACCGCCTTTGCTTCCTTAATGGCGGCACGAATCTCATCA TAGCCATCGTTTCCATTCTTAACCCAGATTGTATGCCACCCAAGAGCCTCAAAGCGAGCACTCA CATCCTCAGTGAATGCAATCTCTGTGTCTCCGTCAATGGAAATGTGGTTGTCATCATAGAATGC AATCAGCTTGCCAAGACCCCAACTCCATCTGGCACCCATCTCCCAAAATACAGTAG Transcript length: 283 Transcript exons: 1810723-1811005 CTTGATTAGTGTTGGCTTGTCAGTTACCGCCTTTGCTTCCTTAATGGCGGCACGAATCTCATCA TAGCCATCGTTTCCATTCTTAACCCAGATTGTATGCCACCCAAGAGCCTCAAAGCGAGCACTCA CATCCTCAGTGAATGCAATCTCTGTGTCTCCGTCAATGGAAATGTGGTTGTCATCATAGAATGC AATCAGCTTGCCAAGACCCCAATGGCCAGCCAACGAGCAAGCTTCATTGGAGATACCCTCCATC TGGCACCCATCTCCCAAAATACAGTAG
Genomic sequence	CTTGATTAGTGTTGGCTTGTCAGTTACCGCCTTTGCTTCCTTAATGGCGGCACGAATCTCATCA TAGCCATCGTTTCCATTCTTAACCCAGATTGTATGCCACCCAAGAGCCTCAAAGCGAGCACTCA CATCCTCAGTGAATGCAATCTCTGTGTCTCCGTCAATGGAAATGTGGTTGTCATCATAGAATGC AATCAGCTTGCCAAGACCCCAATGGCCAGCCAACGAGCAAGCTTCATTGGAGATACCCTCCATC TGGCACCCATCTCCCAAAATACAGTAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_006500* osi_circ_016260 						
					
						PMCS
						1.021761274

Conservation Information
Conserved circRNAs	osi_circ_006500* osi_circ_016260
PMCS	1.021761274

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							1810820-1810724(+)
1810864-1810786(+)
1810979-1810960(-)
						
						Potential amino acid sequence
						
							MPPKSLKASTHILSECNLCVSVNGNVVVIIECNQLAKTPMASQRASFIGDTLHLAPISQNTVA*
(+)
MQSLCLRQWKCGCHHRMQSACQDPNGQPTSKLHWRYPPSGTHLPKYSSLISVGLSVTAFASLMA
ARISS*(+)
MEGISNEACSLAGHWGLGKLIAFYDDNHISIDGDTEIAFTEDVSARFEALGWHTIWVKNGNDGY
DEIRAAIKEAKAVTDKPTLIKLLYFGRWVPDGGYLQ*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	1810820-1810724(+) 1810864-1810786(+) 1810979-1810960(-)
Potential amino acid sequence	MPPKSLKASTHILSECNLCVSVNGNVVVIIECNQLAKTPMASQRASFIGDTLHLAPISQNTVA* (+) MQSLCLRQWKCGCHHRMQSACQDPNGQPTSKLHWRYPPSGTHLPKYSSLISVGLSVTAFASLMA ARISS(+) MEGISNEACSLAGHWGLGKLIAFYDDNHISIDGDTEIAFTEDVSARFEALGWHTIWVKNGNDGY DEIRAAIKEAKAVTDKPTLIKLLYFGRWVPDGGYLQ(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os06g0133800_circ_g.5

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