Plant Circular RNA Database

Detail information of Os03g0370000_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os03g0370000_circ_g.2
					
						ID in PlantcircBase
						osa_circ_019992
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 14527699-14529128  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os03g0370000						
					
						Parent gene annotation
						
							Similar to Pyruvate dehydrogenase kinase isoform 1 (EC 2.7.1.99)
. (Os03t0370000-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0370000-01:3

General Information
CircRNA Name	Os03g0370000_circ_g.2
ID in PlantcircBase	osa_circ_019992
Alias	NA
Organism	Oryza sativa
Position	chr3: 14527699-14529128 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os03g0370000
Parent gene annotation	Similar to Pyruvate dehydrogenase kinase isoform 1 (EC 2.7.1.99) . (Os03t0370000-01)
Parent gene strand	+
Alternative splicing	NA
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0370000-01:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TTCCCTCCGTGCAGTACAGGAACGATATATGAACTCTGATAAGCATGCGCCTCCTGTTAGAATC
ATAGTCGCTGATGGAGCAGAGGACGTAACAATCAAGgtgagagattggtatttggactcattcc
gcgacttacggtgcttcccggaggtgaggaaccgggatgatgagcttgcattcaccgagatgat
caagatga

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GTGAGAGATTGGTATTTGGACTCATTCCGCGACTTACGGTGCTTCCCGGAGGTGAGGAACCGGG
ATGATGAGCTTGCATTCACCGAGATGATCAAGATGATTCGAGTGCGGCACAACAATGTGGTGCC
CACCATGGCGTTGGGTGTGCGGCAGCTGAAGAAAGACCTGGGTGGCACAAAGGCCTTTCCCCCT
GGCATTGATGAGATCCATCAGTTCCTCGACCGCTTCTACATGTCAAGGATTGGGATCCGCATGC
TGATAGGTTAGCAGATTCCGCATGAAGTATTTATTAAATGATGATGGAAATGGAATCTATCTAC
TAATATAATGGCCTTACTTTTAGGCCATTGATATTCCTTGGCCCTTGCCTGCTGGATAAAACTG
GCTAAAAGCCTTGTCAGCACTTAACAATCTGGAAAATATAGTTTTTCTTTGCACATGAATATTT
TATTTATTTTGGTATAACATGTTGGCTATATTAGAGCAGCAATAGCTGTAATGTCCAATCAGGA
TGTCTTTTTCCTCAGAATGTGGTGATTAGAGGATACTCAATCATATCGTTCTAGCTTCTTGTCC
AGAAACTAAAGTTACCACAGAGCAGCTATCAACCATTTACGGTTACATGTCAATAAGAATAATC
CTTTTTTACTTTATTTATGAATGGAAGAAATTGTTTTTCTTTTTTTGATGTGAAAGTGATGCTA
ACATAACAAATAAGTGACAATTGCTACTGCACTTTTTTTTACTGAGTTGTACTAACTTGCTTGA
TTTCAGGGCAGCATGTTGCTTTGCATGAGCCTGATCCAGAACCTGGTGTTATTGGACTCATAAG
CAAAAGATTATCCCCCATGCTGGTGGCTCAACATGCTACTGAAGATGCACGTGCTATTTGCATG
AGGGAATATGGATCAGCCCCTGATGTGAACATATATGGAGACCCAGATTTCACATTTCCGTAAA
TCCCTGATTTAAATACTTGCTACATGTTTTTGTTTAATTTGATTTAATAGCACTTCATTGTTAG
TTCTTCTTTGCTATTAGAAATATTTGTTGGCCACCTAGTTTCTGTTGTCATTTAAATCTTCCAT
GACTTCTACGCAATTGTCAACTAAGCTGCTCTGCTTACTAGTCCATGCACAAATCACATTTTTT
GGCCTTATTTCTGTTTGTCAGAAACCACTCTAGCTGAACAACCTCTATACTGAATTTTAATCAG
AATATGGCTGAAGGACAGGAAGTATTAAAACAGTTGTCCTGAACTGCTTTGCCTTCTTGATTTC
AGATATGTTAAACTACATCTACAACTGATGATGTTTGAATTGGTGAAGAATTCCCTCCGTGCAG
TACAGGAACGATATATGAACTCTGATAAGCATGCGCCTCCTGTTAGAATCATAGTCGCTGATGG
AGCAGAGGACGTAACAATCAAG

Sequences
Splice junction sequence	TTCCCTCCGTGCAGTACAGGAACGATATATGAACTCTGATAAGCATGCGCCTCCTGTTAGAATC ATAGTCGCTGATGGAGCAGAGGACGTAACAATCAAGgtgagagattggtatttggactcattcc gcgacttacggtgcttcccggaggtgaggaaccgggatgatgagcttgcattcaccgagatgat caagatga
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GTGAGAGATTGGTATTTGGACTCATTCCGCGACTTACGGTGCTTCCCGGAGGTGAGGAACCGGG ATGATGAGCTTGCATTCACCGAGATGATCAAGATGATTCGAGTGCGGCACAACAATGTGGTGCC CACCATGGCGTTGGGTGTGCGGCAGCTGAAGAAAGACCTGGGTGGCACAAAGGCCTTTCCCCCT GGCATTGATGAGATCCATCAGTTCCTCGACCGCTTCTACATGTCAAGGATTGGGATCCGCATGC TGATAGGTTAGCAGATTCCGCATGAAGTATTTATTAAATGATGATGGAAATGGAATCTATCTAC TAATATAATGGCCTTACTTTTAGGCCATTGATATTCCTTGGCCCTTGCCTGCTGGATAAAACTG GCTAAAAGCCTTGTCAGCACTTAACAATCTGGAAAATATAGTTTTTCTTTGCACATGAATATTT TATTTATTTTGGTATAACATGTTGGCTATATTAGAGCAGCAATAGCTGTAATGTCCAATCAGGA TGTCTTTTTCCTCAGAATGTGGTGATTAGAGGATACTCAATCATATCGTTCTAGCTTCTTGTCC AGAAACTAAAGTTACCACAGAGCAGCTATCAACCATTTACGGTTACATGTCAATAAGAATAATC CTTTTTTACTTTATTTATGAATGGAAGAAATTGTTTTTCTTTTTTTGATGTGAAAGTGATGCTA ACATAACAAATAAGTGACAATTGCTACTGCACTTTTTTTTACTGAGTTGTACTAACTTGCTTGA TTTCAGGGCAGCATGTTGCTTTGCATGAGCCTGATCCAGAACCTGGTGTTATTGGACTCATAAG CAAAAGATTATCCCCCATGCTGGTGGCTCAACATGCTACTGAAGATGCACGTGCTATTTGCATG AGGGAATATGGATCAGCCCCTGATGTGAACATATATGGAGACCCAGATTTCACATTTCCGTAAA TCCCTGATTTAAATACTTGCTACATGTTTTTGTTTAATTTGATTTAATAGCACTTCATTGTTAG TTCTTCTTTGCTATTAGAAATATTTGTTGGCCACCTAGTTTCTGTTGTCATTTAAATCTTCCAT GACTTCTACGCAATTGTCAACTAAGCTGCTCTGCTTACTAGTCCATGCACAAATCACATTTTTT GGCCTTATTTCTGTTTGTCAGAAACCACTCTAGCTGAACAACCTCTATACTGAATTTTAATCAG AATATGGCTGAAGGACAGGAAGTATTAAAACAGTTGTCCTGAACTGCTTTGCCTTCTTGATTTC AGATATGTTAAACTACATCTACAACTGATGATGTTTGAATTGGTGAAGAATTCCCTCCGTGCAG TACAGGAACGATATATGAACTCTGATAAGCATGCGCCTCCTGTTAGAATCATAGTCGCTGATGG AGCAGAGGACGTAACAATCAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.124081329

Conservation Information
Conserved circRNAs	NA
PMCS	0.124081329

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							14527787-14529125(+)
14527897-14528560(-)
14527779-14529126(-)
						
						Potential amino acid sequence
						
							MIKMIRVRHNNVVPTMALGVRQLKKDLGGTKAFPPGIDEIHQFLDRFYMSRIGIRMLIGQHVAL
HEPDPEPGVIGLISKRLSPMLVAQHATEDARAICMREYGSAPDVNIYGDPDFTFPYVKLHLQLM
MFELVKNSLRAVQERYMNSDKHAPPVRIIVADGAEDVTIKVRDWYLDSFRDLRCFPEVRNRDDE
LAFTEMIKMIRVRHNNVVPTMALGVRQLKKDLGGTKAFPPGIDEIHQFLDRFYMSRIGIRMLIG
QHVALHEPDPEPGVIGLISKRLSPMLVAQHATEDARAICMREYGSAPDVNIYGDPDFTFPYVKL
HLQLMMFELVKNSLRAVQERYMNSDKHAPPVRIIVADGAEDVTIKVRDWYLDSFRDLRCFPEVR
NRDDELAFTEMIKMIRVRHNNVVPTMALGVRQLKKDLGGTKAFPPGIDEIHQFLDRFYMSRIGI
RMLIGQHVALHEPDPEPGVIGLISKRLSPMLVAQHATEDARAICMREYGSAPDVNIYGDPDFTF
PYVKLHLQLMMFELVKNSLRAVQERYMNSDKHAPPVRIIVADGAEDVTIKVRDWYLDSFRDLRC
FPEVRNRDDELAFTEMIKMIRVRHNNVVPTMALGVRQLKKDLGGTKAFPPGIDEIHQFLDRFYM
SRIGIRMLIGQHVALHEPDPEPGVIGLISKRLSPMLVAQHATEDARAICMREYGSAPDVNIYGD
PDFTFPYVKLHLQLMMFELVKNSLRAVQERYMNSDKHAPPVRIIVADGAEDVTIK(+)
MPGGKAFVPPRSFFSCRTPNAMVGTTLLCRTRIILIISVNASSSSRFLTSGKHRKSRNESKYQS
LTLIVTSSAPSATMILTGGACLSEFIYRSCTARREFFTNSNIISCRCSLTYGNVKSGSPYMFTS
GADPYSLMQIARASSVAC*(-)
MQAHHPGSSPPGSTVSRGMSPNTNLSP*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	14527787-14529125(+) 14527897-14528560(-) 14527779-14529126(-)
Potential amino acid sequence	MIKMIRVRHNNVVPTMALGVRQLKKDLGGTKAFPPGIDEIHQFLDRFYMSRIGIRMLIGQHVAL HEPDPEPGVIGLISKRLSPMLVAQHATEDARAICMREYGSAPDVNIYGDPDFTFPYVKLHLQLM MFELVKNSLRAVQERYMNSDKHAPPVRIIVADGAEDVTIKVRDWYLDSFRDLRCFPEVRNRDDE LAFTEMIKMIRVRHNNVVPTMALGVRQLKKDLGGTKAFPPGIDEIHQFLDRFYMSRIGIRMLIG QHVALHEPDPEPGVIGLISKRLSPMLVAQHATEDARAICMREYGSAPDVNIYGDPDFTFPYVKL HLQLMMFELVKNSLRAVQERYMNSDKHAPPVRIIVADGAEDVTIKVRDWYLDSFRDLRCFPEVR NRDDELAFTEMIKMIRVRHNNVVPTMALGVRQLKKDLGGTKAFPPGIDEIHQFLDRFYMSRIGI RMLIGQHVALHEPDPEPGVIGLISKRLSPMLVAQHATEDARAICMREYGSAPDVNIYGDPDFTF PYVKLHLQLMMFELVKNSLRAVQERYMNSDKHAPPVRIIVADGAEDVTIKVRDWYLDSFRDLRC FPEVRNRDDELAFTEMIKMIRVRHNNVVPTMALGVRQLKKDLGGTKAFPPGIDEIHQFLDRFYM SRIGIRMLIGQHVALHEPDPEPGVIGLISKRLSPMLVAQHATEDARAICMREYGSAPDVNIYGD PDFTFPYVKLHLQLMMFELVKNSLRAVQERYMNSDKHAPPVRIIVADGAEDVTIK(+) MPGGKAFVPPRSFFSCRTPNAMVGTTLLCRTRIILIISVNASSSSRFLTSGKHRKSRNESKYQS LTLIVTSSAPSATMILTGGACLSEFIYRSCTARREFFTNSNIISCRCSLTYGNVKSGSPYMFTS GADPYSLMQIARASSVAC(-) MQAHHPGSSPPGSTVSRGMSPNTNLSP(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os03g0370000_circ_g.2

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