Plant Circular RNA Database

Detail information of Os05g0481500_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os05g0481500_circ_g.1
					
						ID in PlantcircBase
						osa_circ_028260
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 23676154-23676693  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os05g0481500						
					
						Parent gene annotation
						
							K Homology domain containing protein. (Os05t0481500-01);K Homolo
gy domain containing protein. (Os05t0481500-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0481500-02:2
Os05t0481500-01:2

General Information
CircRNA Name	Os05g0481500_circ_g.1
ID in PlantcircBase	osa_circ_028260
Alias	NA
Organism	Oryza sativa
Position	chr5: 23676154-23676693 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os05g0481500
Parent gene annotation	K Homology domain containing protein. (Os05t0481500-01);K Homolo gy domain containing protein. (Os05t0481500-02)
Parent gene strand	-
Alternative splicing	NA
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0481500-02:2 Os05t0481500-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GAGCTCCTCACCTTGATTCAACAGCCTGCTGCAGATTGGGAGCACCTGCATGAACGGCCCCAGC
TTCTGATGCTCCTGCAACAACTCCGCCAAGTACTGCctctggacgcatcccattccatggacca
aaagcattaccacagaaattgggcacgatgtgcgacggatgcatctgatttgggcttgcaggtt
gcgacctc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 241
Transcript exons: 23676154-23676305,23676605-23676693
CTCTGGACGCATCCCATTCCATGGACCAAAAGCATTACCACAGAAATTGGGCACGATGTGCGAC
GGATGCATCTGATTTGGGCTTGCAGGTTGCGACCTCTCGAAGTCCCCTACGCCGGGTTGCCGAA
ACATCCCAGACACTCGCATTATCTCTTGATTCAACAGCCTGCTGCAGATTGGGAGCACCTGCAT
GAACGGCCCCAGCTTCTGATGCTCCTGCAACAACTCCGCCAAGTACTGC
						
						Genomic sequence
						
							CTCTGGACGCATCCCATTCCATGGACCAAAAGCATTACCACAGAAATTGGGCACGATGTGCGAC
GGATGCATCTGATTTGGGCTTGCAGGTTGCGACCTCTCGAAGTCCCCTACGCCGGGTTGCCGAA
ACATCCCAGACACTCGCATTATCTCTGTCAGCAGCAAAACATTAGCACATGGCATCAGCATGGA
CACACATGAAAACACTTGATTGCCACTGAAGAAAAGATACACTGATACAGTCTCTTGTCAAGAA
TCAAGCTACAACCTTTCAAGCCCTACGAAACACACTAGGATTGCGCATAGACCCCGATCGAGCT
AAGTTTCGCATCATCGAGCAGGGACGAGCAAGAGCAAATTAGCCAAGGCCGTGGAATCGGAGAA
GACGAACACTCCAATCCGATCCAAGAATGCTATCCACTCAGTAAGCAGGGCGAGCAGAGCTCCT
CACCTTGATTCAACAGCCTGCTGCAGATTGGGAGCACCTGCATGAACGGCCCCAGCTTCTGATG
CTCCTGCAACAACTCCGCCAAGTACTGC

Sequences
Splice junction sequence	GAGCTCCTCACCTTGATTCAACAGCCTGCTGCAGATTGGGAGCACCTGCATGAACGGCCCCAGC TTCTGATGCTCCTGCAACAACTCCGCCAAGTACTGCctctggacgcatcccattccatggacca aaagcattaccacagaaattgggcacgatgtgcgacggatgcatctgatttgggcttgcaggtt gcgacctc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 241 Transcript exons: 23676154-23676305,23676605-23676693 CTCTGGACGCATCCCATTCCATGGACCAAAAGCATTACCACAGAAATTGGGCACGATGTGCGAC GGATGCATCTGATTTGGGCTTGCAGGTTGCGACCTCTCGAAGTCCCCTACGCCGGGTTGCCGAA ACATCCCAGACACTCGCATTATCTCTTGATTCAACAGCCTGCTGCAGATTGGGAGCACCTGCAT GAACGGCCCCAGCTTCTGATGCTCCTGCAACAACTCCGCCAAGTACTGC
Genomic sequence	CTCTGGACGCATCCCATTCCATGGACCAAAAGCATTACCACAGAAATTGGGCACGATGTGCGAC GGATGCATCTGATTTGGGCTTGCAGGTTGCGACCTCTCGAAGTCCCCTACGCCGGGTTGCCGAA ACATCCCAGACACTCGCATTATCTCTGTCAGCAGCAAAACATTAGCACATGGCATCAGCATGGA CACACATGAAAACACTTGATTGCCACTGAAGAAAAGATACACTGATACAGTCTCTTGTCAAGAA TCAAGCTACAACCTTTCAAGCCCTACGAAACACACTAGGATTGCGCATAGACCCCGATCGAGCT AAGTTTCGCATCATCGAGCAGGGACGAGCAAGAGCAAATTAGCCAAGGCCGTGGAATCGGAGAA GACGAACACTCCAATCCGATCCAAGAATGCTATCCACTCAGTAAGCAGGGCGAGCAGAGCTCCT CACCTTGATTCAACAGCCTGCTGCAGATTGGGAGCACCTGCATGAACGGCCCCAGCTTCTGATG CTCCTGCAACAACTCCGCCAAGTACTGC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.206419985

Conservation Information
Conserved circRNAs	NA
PMCS	0.206419985

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							23676221-23676643(+)
23676177-23676613(-)
23676646-23676190(-)
23676190-23676190(-)
						
						Potential amino acid sequence
						
							MHLIWACRLRPLEVPYAGLPKHPRHSHYLLIQQPAADWEHLHERPQLLMLLQQLRQVLPLDASH
SMDQKHYHRNWARCATDASDLGLQVATSRSPLRRVAETSQTLALSLDSTACCRLGAPA*(+)
MEWDASRGSTWRSCCRSIRSWGRSCRCSQSAAGC*(-)
MQVLPICSRLLNQEIMRVSGMFRQPGVGDFERSQPASPNQMHPSHIVPNFCGNAFGPWNGMRPE
AVLGGVVAGASEAGAVHAGAPNLQQAVESRDNASVWDVSATRRRGLREVATCKPKSDASVAHRA
QFLW*(-)
MLLVHGMGCVQRQYLAELLQEHQKLGPFMQVLPICSRLLNQEIMRVSGMFRQPGVGDFERSQPA
SPNQMHPSHIVPNFCGNAFGPWNGMRPEAVLGGVVAGASEAGAVHAGAPNLQQAVESRDNASVW
DVSATRRRGLREVATCKPKSDASVAHRAQFLW*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	23676221-23676643(+) 23676177-23676613(-) 23676646-23676190(-) 23676190-23676190(-)
Potential amino acid sequence	MHLIWACRLRPLEVPYAGLPKHPRHSHYLLIQQPAADWEHLHERPQLLMLLQQLRQVLPLDASH SMDQKHYHRNWARCATDASDLGLQVATSRSPLRRVAETSQTLALSLDSTACCRLGAPA(+) MEWDASRGSTWRSCCRSIRSWGRSCRCSQSAAGC(-) MQVLPICSRLLNQEIMRVSGMFRQPGVGDFERSQPASPNQMHPSHIVPNFCGNAFGPWNGMRPE AVLGGVVAGASEAGAVHAGAPNLQQAVESRDNASVWDVSATRRRGLREVATCKPKSDASVAHRA QFLW(-) MLLVHGMGCVQRQYLAELLQEHQKLGPFMQVLPICSRLLNQEIMRVSGMFRQPGVGDFERSQPA SPNQMHPSHIVPNFCGNAFGPWNGMRPEAVLGGVVAGASEAGAVHAGAPNLQQAVESRDNASVW DVSATRRRGLREVATCKPKSDASVAHRAQFLW(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os05g0481500_circ_g.1

Address

Any questions?

Supported by