Plant Circular RNA Database

Detail information of AT2G45380_circ_g.2

			
						 General Information
					
						CircRNA Name
						AT2G45380_circ_g.2
					
						ID in PlantcircBase
						ath_circ_018512
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr2: 18699814-18700487  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							AT2G45380						
					
						Parent gene annotation
						
							BEST Arabidopsis thaliana protein match is: Calcium-binding EF-h
and family protein (TAIR:AT4G34070.1)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							AT2G45380_circ_g.1 						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT2G45380.4:4
AT2G45380.1:4
AT2G45380.2:4
AT2G45380.3:4

General Information
CircRNA Name	AT2G45380_circ_g.2
ID in PlantcircBase	ath_circ_018512
Alias	NA
Organism	Arabidpsis thaliana
Position	chr2: 18699814-18700487 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	AT2G45380
Parent gene annotation	BEST Arabidopsis thaliana protein match is: Calcium-binding EF-h and family protein (TAIR:AT4G34070.1)
Parent gene strand	-
Alternative splicing	AT2G45380_circ_g.1
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT2G45380.4:4 AT2G45380.1:4 AT2G45380.2:4 AT2G45380.3:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ACCTTAGAGATGTGATGTCTGAATGTGTTAGCTCGAGACTGTGTACCCTCTCTTCGGTTAAAAT
CACTCCTTTGATTCACATTCTGAATCTTCCTCTCAGcagtgaattcatgcttgccaagagatga
attgcctttccatgcttcttcaaagccacttaattcatctaaaacaaaaaaattaaagaaacga
tcttttag

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 390
Transcript exons: 18699814-18699879,18699978-18700053,18700166-18700246,18
700321-18700487
CAGTGAATTCATGCTTGCCAAGAGATGAATTGCCTTTCCATGCTTCTTCAAAGCCACTTAATTC
ATCTTCATCGAGGTTGTGAAGAGTCTGTGTCGACTCTACACCACCACTTGAGTTGAGCTTCCTA
GTAACTGAATGACCCTTATCATTGATTCCTCTTGAGATCCTATGAGTGGCTTCACCGGTAGTCT
TATCAGCTTCTTTGCTTTCCTCAACCACCATTCCATCACCGTCTTTTCTTCTGGTTCTAGTAGA
TGTATAGTACGCACCATGTATGCCACCATATGTAACCTTAGAGATGTGATGTCTGAATGTGTTA
GCTCGAGACTGTGTACCCTCTCTTCGGTTAAAATCACTCCTTTGATTCACATTCTGAATCTTCC
TCTCAG
						
						Genomic sequence
						
							CAGTGAATTCATGCTTGCCAAGAGATGAATTGCCTTTCCATGCTTCTTCAAAGCCACTTAATTC
ATCTAAAACAAAAAAATTAAAGAAACGATCTTTTAGAATCCTGCCAGTTTTCTTTCAAAGAACT
TTAAGTTATGATAAGGCTGTGTAGATTCTGCAGGACCTTCATCGAGGTTGTGAAGAGTCTGTGT
CGACTCTACACCACCACTTGAGTTGAGCTTCCTAGTAACTGAATGACCCTGAATACACCAAACC
AACAGTCTCAGACTCGAGAGCCAAGACTCATCTAAAACTGAACCTTGGTGGAACCAAGAAGTGA
TGGAGCAAAACATCAACAGTGTAATCAATCACCTTATCATTGATTCCTCTTGAGATCCTATGAG
TGGCTTCACCGGTAGTCTTATCAGCTTCTTTGCTTTCCTCAACCACCATCTAGAAACAGAATCT
GATCAGTATGTGTAAAACTTCTACTAATAGAAAGGATCGGAAAATAGAAACAAACTTACTCCAT
CACCGTCTTTTCTTCTGGTTCTAGTAGATGTATAGTACGCACCATGTATGCCACCATATGTAAC
CTTAGAGATGTGATGTCTGAATGTGTTAGCTCGAGACTGTGTACCCTCTCTTCGGTTAAAATCA
CTCCTTTGATTCACATTCTGAATCTTCCTCTCAG

Sequences
Splice junction sequence	ACCTTAGAGATGTGATGTCTGAATGTGTTAGCTCGAGACTGTGTACCCTCTCTTCGGTTAAAAT CACTCCTTTGATTCACATTCTGAATCTTCCTCTCAGcagtgaattcatgcttgccaagagatga attgcctttccatgcttcttcaaagccacttaattcatctaaaacaaaaaaattaaagaaacga tcttttag
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 390 Transcript exons: 18699814-18699879,18699978-18700053,18700166-18700246,18 700321-18700487 CAGTGAATTCATGCTTGCCAAGAGATGAATTGCCTTTCCATGCTTCTTCAAAGCCACTTAATTC ATCTTCATCGAGGTTGTGAAGAGTCTGTGTCGACTCTACACCACCACTTGAGTTGAGCTTCCTA GTAACTGAATGACCCTTATCATTGATTCCTCTTGAGATCCTATGAGTGGCTTCACCGGTAGTCT TATCAGCTTCTTTGCTTTCCTCAACCACCATTCCATCACCGTCTTTTCTTCTGGTTCTAGTAGA TGTATAGTACGCACCATGTATGCCACCATATGTAACCTTAGAGATGTGATGTCTGAATGTGTTA GCTCGAGACTGTGTACCCTCTCTTCGGTTAAAATCACTCCTTTGATTCACATTCTGAATCTTCC TCTCAG
Genomic sequence	CAGTGAATTCATGCTTGCCAAGAGATGAATTGCCTTTCCATGCTTCTTCAAAGCCACTTAATTC ATCTAAAACAAAAAAATTAAAGAAACGATCTTTTAGAATCCTGCCAGTTTTCTTTCAAAGAACT TTAAGTTATGATAAGGCTGTGTAGATTCTGCAGGACCTTCATCGAGGTTGTGAAGAGTCTGTGT CGACTCTACACCACCACTTGAGTTGAGCTTCCTAGTAACTGAATGACCCTGAATACACCAAACC AACAGTCTCAGACTCGAGAGCCAAGACTCATCTAAAACTGAACCTTGGTGGAACCAAGAAGTGA TGGAGCAAAACATCAACAGTGTAATCAATCACCTTATCATTGATTCCTCTTGAGATCCTATGAG TGGCTTCACCGGTAGTCTTATCAGCTTCTTTGCTTTCCTCAACCACCATCTAGAAACAGAATCT GATCAGTATGTGTAAAACTTCTACTAATAGAAAGGATCGGAAAATAGAAACAAACTTACTCCAT CACCGTCTTTTCTTCTGGTTCTAGTAGATGTATAGTACGCACCATGTATGCCACCATATGTAAC CTTAGAGATGTGATGTCTGAATGTGTTAGCTCGAGACTGTGTACCCTCTCTTCGGTTAAAATCA CTCCTTTGATTCACATTCTGAATCTTCCTCTCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.218945445

Conservation Information
Conserved circRNAs	NA
PMCS	0.218945445

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							18700247-18699816(-)
						
						Potential amino acid sequence
						
							MVVEESKEADKTTGEATHRISRGINDKGHSVTRKLNSSGGVESTQTLHNLDEDELSGFEEAWKG
NSSLGKHEFTAERKIQNVNQRSDFNRREGTQSRANTFRHHISKVTYGGIHGAYYTSTRTRRKDG
DGMVVEESKEADKTTGEATHRISRGINDKGHSVTRKLNSSGGVESTQTLHNLDEDELSGFEEAW
KGNSSLGKHEFTAERKIQNVNQRSDFNRREGTQSRANTFRHHISKVTYGGIHGAYYTSTRTRRK
DGDGMVVEESKEADKTTGEATHRISRGINDKGHSVTRKLNSSGGVESTQTLHNLDEDELSGFEE
AWKGNSSLGKHEFTAERKIQNVNQRSDFNRREGTQSRANTFRHHISKVTYGGIHGAYYTSTRTR
RKDGDGMVVEESKEADKTTGEATHRISRGINDKGHSVTRKLNSSGGVESTQTLHNLDEDELSGF
EEAWKGNSSLGKHEFT(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	18700247-18699816(-)
Potential amino acid sequence	MVVEESKEADKTTGEATHRISRGINDKGHSVTRKLNSSGGVESTQTLHNLDEDELSGFEEAWKG NSSLGKHEFTAERKIQNVNQRSDFNRREGTQSRANTFRHHISKVTYGGIHGAYYTSTRTRRKDG DGMVVEESKEADKTTGEATHRISRGINDKGHSVTRKLNSSGGVESTQTLHNLDEDELSGFEEAW KGNSSLGKHEFTAERKIQNVNQRSDFNRREGTQSRANTFRHHISKVTYGGIHGAYYTSTRTRRK DGDGMVVEESKEADKTTGEATHRISRGINDKGHSVTRKLNSSGGVESTQTLHNLDEDELSGFEE AWKGNSSLGKHEFTAERKIQNVNQRSDFNRREGTQSRANTFRHHISKVTYGGIHGAYYTSTRTR RKDGDGMVVEESKEADKTTGEATHRISRGINDKGHSVTRKLNSSGGVESTQTLHNLDEDELSGF EEAWKGNSSLGKHEFT(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of AT2G45380_circ_g.2

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