Plant Circular RNA Database

Detail information of Zm00001d023262_circ_g.2

			
						 General Information
					
						CircRNA Name
						Zm00001d023262_circ_g.2
					
						ID in PlantcircBase
						zma_circ_010132
					
						Alias
						Zm10circ00002, ZmciR446
					
						Organism
						
							Zea mays						
					
						Position
						
							chr10: 1576506-1577984  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRI2
					
						Parent gene
						
							Zm00001d023262						
					
						Parent gene annotation
						
							 brick3
							
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						NA
					
						Tissues
						leaf, endosperm
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d023262_T014:5
Zm00001d023262_T018:5
Zm00001d023262_T032:5
Zm00001d023262_T003:5
Zm00001d023262_T011:5
Zm00001d023262_T026:5
Zm00001d023262_T027:5
Zm00001d023262_T022:5
Zm00001d023262_T047:5
Zm00001d023262_T058:5
Zm00001d023262_T033:5
Zm00001d023262_T052:5
Zm00001d023262_T012:5
Zm00001d023262_T001:5
Zm00001d023262_T024:5
Zm00001d023262_T039:5
Zm00001d023262_T009:5
Zm00001d023262_T007:5
Zm00001d023262_T068:5
Zm00001d023262_T028:5
Zm00001d023262_T023:3
Zm00001d023262_T038:5
Zm00001d023262_T043:5
Zm00001d023262_T031:1
Zm00001d023262_T016:5
Zm00001d023262_T029:5
Zm00001d023262_T035:5
Zm00001d023262_T017:5
Zm00001d023262_T021:5
Zm00001d023262_T036:5
Zm00001d023262_T020:5
Zm00001d023262_T034:5
Zm00001d023262_T030:5
Zm00001d023262_T010:5
Zm00001d023262_T019:5
Zm00001d023262_T025:5
Zm00001d023262_T006:4
Zm00001d023262_T008:5
Zm00001d023262_T005:4
Zm00001d023262_T015:5
Zm00001d023262_T004:5
Zm00001d023262_T002:5
Zm00001d023262_T013:5

General Information
CircRNA Name	Zm00001d023262_circ_g.2
ID in PlantcircBase	zma_circ_010132
Alias	Zm10circ00002, ZmciR446
Organism	Zea mays
Position	chr10: 1576506-1577984 JBrowse»
Reference genome	AGPv4.38
Type	u-circRNA
Identification method	CIRI2
Parent gene	Zm00001d023262
Parent gene annotation	brick3
Parent gene strand	-
Alternative splicing	NA
Support reads	NA
Tissues	leaf, endosperm
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d023262_T014:5 Zm00001d023262_T018:5 Zm00001d023262_T032:5 Zm00001d023262_T003:5 Zm00001d023262_T011:5 Zm00001d023262_T026:5 Zm00001d023262_T027:5 Zm00001d023262_T022:5 Zm00001d023262_T047:5 Zm00001d023262_T058:5 Zm00001d023262_T033:5 Zm00001d023262_T052:5 Zm00001d023262_T012:5 Zm00001d023262_T001:5 Zm00001d023262_T024:5 Zm00001d023262_T039:5 Zm00001d023262_T009:5 Zm00001d023262_T007:5 Zm00001d023262_T068:5 Zm00001d023262_T028:5 Zm00001d023262_T023:3 Zm00001d023262_T038:5 Zm00001d023262_T043:5 Zm00001d023262_T031:1 Zm00001d023262_T016:5 Zm00001d023262_T029:5 Zm00001d023262_T035:5 Zm00001d023262_T017:5 Zm00001d023262_T021:5 Zm00001d023262_T036:5 Zm00001d023262_T020:5 Zm00001d023262_T034:5 Zm00001d023262_T030:5 Zm00001d023262_T010:5 Zm00001d023262_T019:5 Zm00001d023262_T025:5 Zm00001d023262_T006:4 Zm00001d023262_T008:5 Zm00001d023262_T005:4 Zm00001d023262_T015:5 Zm00001d023262_T004:5 Zm00001d023262_T002:5 Zm00001d023262_T013:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TGTCTGGATAACGTGGATGAAATGGACTGAGGAAACCTTCGTTCCTTAACTTTTTTGTATCAGT
TGAAAGAAATATAATTGGGCCAACAGCTTCCAGGACctgaatattaggtgctaggaggctaggt
tgatcagtgaaaaataaaaccatccttccaatttcctgcttaagaagaattctcctctcacgat
gcatagca

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 555
Transcript exons: 1576506-1576638,1576971-1577113,1577203-1577247,1577625-
1577732,1577859-1577984
CTGAATATTAGGTGCTAGGAGGCTAGGTTGATCAGTGAAAAATAAAACCATCCTTCCAATTTCC
TGCTTAAGAAGAATTCTCCTCTCACGATGCATAGCATCACATGACACCAGTGCTTGCTCATGCA
CTTCCGTTAACATTCTCTCAACCTGCTTTGCAACATTGTACTCCAGATCAGCTTCCTTTTGCTT
AGTTCGGCCAGATTTAGCCATTCTCTTCGATTCTAGAACTTTTGGAAGAACATAAAGTTGATAA
TTTTCATGAAGTAAGATGTACTCATCTCTGAACAGAGGAAGTATTAAGTTCTCTTTCAGAACAA
CCATGGCAACATCTATACTGGTCACCCTAAGTAGTTCATCAGGACAAACAAGATATCCTAGAAG
AACCCACTCACGGTATGATGTGACATTAGCCAAGTCTTGGGCCCTCATTGGATGGGCAGAATTT
GTTAGGATGTCTGGATAACGTGGATGAAATGGACTGAGGAAACCTTCGTTCCTTAACTTTTTTG
TATCAGTTGAAAGAAATATAATTGGGCCAACAGCTTCCAGGAC
						
					

						Genomic sequence
						
							CTGAATATTAGGTGCTAGGAGGCTAGGTTGATCAGTGAAAAATAAAACCATCCTTCCAATTTCC
TGCTTAAGAAGAATTCTCCTCTCACGATGCATAGCATCACATGACACCAGTGCTTGCTCATGCA
CTTCCCTGTAGAGAGTTAACACAAACCATTAACAAACATCTGAAGAATGATGCCAAGTAGATAA
AATAAAAAAGAACAAATTAAAAGTAATGAAACCAGAAGGGTAAAATGCTAGGTAAAATAGTTCC
CGAGTTATGAAGCCAAAAGAACCAACCAAGAATAGGCTATCTGAACTAGTGGCAAATCCCAAAA
GAAGTGAATATGCAGAAAGATTTCAAAAACAATAATCAAAATATAAGGTAAATAACTCAAACTT
GTGGAACAGAACCATCTTCACATTACAAGCTATTCTGGAGCATAGAGAAAAGTATATATACAAT
GACTGTGCAGGACATACGTTAACATTCTCTCAACCTGCTTTGCAACATTGTACTCCAGATCAGC
TTCCTTTTGCTTAGTTCGGCCAGATTTAGCCATTCTCTTCGATTCTAGAACTTTTGGAAGAACA
TAAAGTTGATAATTTTCATGAAGTAAGATGTACTACACAAAAAATGAAAAAAAAAACAAATTAG
AATAGTGAATAGTGACACCAAAGAATTAATCTTGCTGCCACATGTCTTTAATTTTACCTCATCT
CTGAACAGAGGAAGTATTAAGTTCTCTTTCAGAACAACCTGAAAGTGACCAAGAAGAAACAGAT
CAAGGCTAAATCACAGATGACATGCTGGTTGCACTGAGGAATCTAGTTTGGTGAATCTTTACTA
ATTCTAGTACAGTTTCTTGTAACTGATTCATGAAAGATGTCTTCAACCAAATCCATCTAAAAAT
AGAAATAAATGAATGCTTTTAAAAAGCCAACCATAGCACCAAACGGAGGCTTACCAGATACCAA
CACAAGACTTATTATGATAAAATGGAGTGAGAAAACACGATGCATGTGCCACTATTTTGGTTGA
TGTAAGTGCAACACTAAAACCATTATATACACCATAAATTATTAGCTATTCAGGAAAAGAGAGT
AGAAACTGACAAACAAAGACATAGGGCATACCATGGCAACATCTATACTGGTCACCCTAAGTAG
TTCATCAGGACAAACAAGATATCCTAGAAGAACCCACTCACGGTATGATGTGACATTAGCCAAG
TCTTGGGCCCTCTGGGAGGAAACAGAATCAGGTTAAATCTCTAAATTACAATACATTGTTATCA
TGGTATTGGCAGAATAATGGACAGACATTTATGCTCTCATGATTTATCATCTCTAACAGTGAAA
GGTACTAACCATTGGATGGGCAGAATTTGTTAGGATGTCTGGATAACGTGGATGAAATGGACTG
AGGAAACCTTCGTTCCTTAACTTTTTTGTATCAGTTGAAAGAAATATAATTGGGCCAACAGCTT
CCAGGAC
						
					

			
		

Sequences
Splice junction sequence	TGTCTGGATAACGTGGATGAAATGGACTGAGGAAACCTTCGTTCCTTAACTTTTTTGTATCAGT TGAAAGAAATATAATTGGGCCAACAGCTTCCAGGACctgaatattaggtgctaggaggctaggt tgatcagtgaaaaataaaaccatccttccaatttcctgcttaagaagaattctcctctcacgat gcatagca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 555 Transcript exons: 1576506-1576638,1576971-1577113,1577203-1577247,1577625- 1577732,1577859-1577984 CTGAATATTAGGTGCTAGGAGGCTAGGTTGATCAGTGAAAAATAAAACCATCCTTCCAATTTCC TGCTTAAGAAGAATTCTCCTCTCACGATGCATAGCATCACATGACACCAGTGCTTGCTCATGCA CTTCCGTTAACATTCTCTCAACCTGCTTTGCAACATTGTACTCCAGATCAGCTTCCTTTTGCTT AGTTCGGCCAGATTTAGCCATTCTCTTCGATTCTAGAACTTTTGGAAGAACATAAAGTTGATAA TTTTCATGAAGTAAGATGTACTCATCTCTGAACAGAGGAAGTATTAAGTTCTCTTTCAGAACAA CCATGGCAACATCTATACTGGTCACCCTAAGTAGTTCATCAGGACAAACAAGATATCCTAGAAG AACCCACTCACGGTATGATGTGACATTAGCCAAGTCTTGGGCCCTCATTGGATGGGCAGAATTT GTTAGGATGTCTGGATAACGTGGATGAAATGGACTGAGGAAACCTTCGTTCCTTAACTTTTTTG TATCAGTTGAAAGAAATATAATTGGGCCAACAGCTTCCAGGAC
Genomic sequence	CTGAATATTAGGTGCTAGGAGGCTAGGTTGATCAGTGAAAAATAAAACCATCCTTCCAATTTCC TGCTTAAGAAGAATTCTCCTCTCACGATGCATAGCATCACATGACACCAGTGCTTGCTCATGCA CTTCCCTGTAGAGAGTTAACACAAACCATTAACAAACATCTGAAGAATGATGCCAAGTAGATAA AATAAAAAAGAACAAATTAAAAGTAATGAAACCAGAAGGGTAAAATGCTAGGTAAAATAGTTCC CGAGTTATGAAGCCAAAAGAACCAACCAAGAATAGGCTATCTGAACTAGTGGCAAATCCCAAAA GAAGTGAATATGCAGAAAGATTTCAAAAACAATAATCAAAATATAAGGTAAATAACTCAAACTT GTGGAACAGAACCATCTTCACATTACAAGCTATTCTGGAGCATAGAGAAAAGTATATATACAAT GACTGTGCAGGACATACGTTAACATTCTCTCAACCTGCTTTGCAACATTGTACTCCAGATCAGC TTCCTTTTGCTTAGTTCGGCCAGATTTAGCCATTCTCTTCGATTCTAGAACTTTTGGAAGAACA TAAAGTTGATAATTTTCATGAAGTAAGATGTACTACACAAAAAATGAAAAAAAAAACAAATTAG AATAGTGAATAGTGACACCAAAGAATTAATCTTGCTGCCACATGTCTTTAATTTTACCTCATCT CTGAACAGAGGAAGTATTAAGTTCTCTTTCAGAACAACCTGAAAGTGACCAAGAAGAAACAGAT CAAGGCTAAATCACAGATGACATGCTGGTTGCACTGAGGAATCTAGTTTGGTGAATCTTTACTA ATTCTAGTACAGTTTCTTGTAACTGATTCATGAAAGATGTCTTCAACCAAATCCATCTAAAAAT AGAAATAAATGAATGCTTTTAAAAAGCCAACCATAGCACCAAACGGAGGCTTACCAGATACCAA CACAAGACTTATTATGATAAAATGGAGTGAGAAAACACGATGCATGTGCCACTATTTTGGTTGA TGTAAGTGCAACACTAAAACCATTATATACACCATAAATTATTAGCTATTCAGGAAAAGAGAGT AGAAACTGACAAACAAAGACATAGGGCATACCATGGCAACATCTATACTGGTCACCCTAAGTAG TTCATCAGGACAAACAAGATATCCTAGAAGAACCCACTCACGGTATGATGTGACATTAGCCAAG TCTTGGGCCCTCTGGGAGGAAACAGAATCAGGTTAAATCTCTAAATTACAATACATTGTTATCA TGGTATTGGCAGAATAATGGACAGACATTTATGCTCTCATGATTTATCATCTCTAACAGTGAAA GGTACTAACCATTGGATGGGCAGAATTTGTTAGGATGTCTGGATAACGTGGATGAAATGGACTG AGGAAACCTTCGTTCCTTAACTTTTTTGTATCAGTTGAAAGAAATATAATTGGGCCAACAGCTT CCAGGAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.180865589

Conservation Information
Conserved circRNAs	NA
PMCS	0.180865589

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							1577863-1576508(-)
						
						Potential amino acid sequence
						
							MRAQDLANVTSYREWVLLGYLVCPDELLRVTSIDVAMVVLKENLILPLFRDEYILLHENYQLYV
LPKVLESKRMAKSGRTKQKEADLEYNVAKQVERMLTEVHEQALVSCDAMHRERRILLKQEIGRM
VLFFTDQPSLLAPNIQVLEAVGPIIFLSTDTKKLRNEGFLSPFHPRYPDILTNSAHPMRAQDLA
NVTSYREWVLLGYLVCPDELLRVTSIDVAMVVLKENLILPLFRDEYILLHENYQLYVLPKVLES
KRMAKSGRTKQKEADLEYNVAKQVERMLTEVHEQALVSCDAMHRERRILLKQEIGRMVLFFTDQ
PSLLAPNIQVLEAVGPIIFLSTDTKKLRNEGFLSPFHPRYPDILTNSAHPMRAQDLANVTSYRE
WVLLGYLVCPDELLRVTSIDVAMVVLKENLILPLFRDEYILLHENYQLYVLPKVLESKRMAKSG
RTKQKEADLEYNVAKQVERMLTEVHEQALVSCDAMHRERRILLKQEIGRMVLFFTDQPSLLAPN
IQVLEAVGPIIFLSTDTKKLRNEGFLSPFHPRYPDILTNSAHPMRAQDLANVTSYREWVLLGYL
VCPDELLRVTSIDVAMVVLKENLILPLFRDEYILLHENYQLYVLPKVLESKRMAKSGRTKQKEA
DLEYNVAKQVERMLTEVHEQALVSCDAMHRERRILLKQEIGRMVLFFTDQPSLLAPNIQ(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to drought stress

Functional Information
Coding potential	Y
Potential coding position	1577863-1576508(-)
Potential amino acid sequence	MRAQDLANVTSYREWVLLGYLVCPDELLRVTSIDVAMVVLKENLILPLFRDEYILLHENYQLYV LPKVLESKRMAKSGRTKQKEADLEYNVAKQVERMLTEVHEQALVSCDAMHRERRILLKQEIGRM VLFFTDQPSLLAPNIQVLEAVGPIIFLSTDTKKLRNEGFLSPFHPRYPDILTNSAHPMRAQDLA NVTSYREWVLLGYLVCPDELLRVTSIDVAMVVLKENLILPLFRDEYILLHENYQLYVLPKVLES KRMAKSGRTKQKEADLEYNVAKQVERMLTEVHEQALVSCDAMHRERRILLKQEIGRMVLFFTDQ PSLLAPNIQVLEAVGPIIFLSTDTKKLRNEGFLSPFHPRYPDILTNSAHPMRAQDLANVTSYRE WVLLGYLVCPDELLRVTSIDVAMVVLKENLILPLFRDEYILLHENYQLYVLPKVLESKRMAKSG RTKQKEADLEYNVAKQVERMLTEVHEQALVSCDAMHRERRILLKQEIGRMVLFFTDQPSLLAPN IQVLEAVGPIIFLSTDTKKLRNEGFLSPFHPRYPDILTNSAHPMRAQDLANVTSYREWVLLGYL VCPDELLRVTSIDVAMVVLKENLILPLFRDEYILLHENYQLYVLPKVLESKRMAKSGRTKQKEA DLEYNVAKQVERMLTEVHEQALVSCDAMHRERRILLKQEIGRMVLFFTDQPSLLAPNIQ(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to drought stress

			
						 Other Information
					
						References
						Han et al., 2020;Zhang et al., 2019

circRNA

Detail information of Zm00001d023262_circ_g.2

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