Plant Circular RNA Database

Detail information of Csa4G108510_circ_g.5

			
						 General Information
					
						CircRNA Name
						Csa4G108510_circ_g.5
					
						ID in PlantcircBase
						csa_circ_002464
					
						Alias
						Chr4:6967292_6973892
					
						Organism
						
							Cucumis sativus						
					
						Position
						
							chrChr4: 6967292-6973892  JBrowse»						
					
						Reference genome
						
							Cucumis sativus v1.5						
					
						Type  
						
							e-circRNA							
									
						Identification method
						Find_circ
					
						Parent gene
						
							Csa4G108510						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						-
					
						Alternative splicing
						
							Chr4_circ_ig.30 Chr4_circ_ig.31 Chr4_circ_ig.32 Chr4_circ_ig.33 Csa4G108510_circ_g.1 Csa4G108510_circ_g.2 Csa4G108510_circ_g.3 Csa4G108510_circ_g.4 						
					
						Support reads
						NA
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Csa4G108510.1:9
Csa4G108510.2:9

General Information
CircRNA Name	Csa4G108510_circ_g.5
ID in PlantcircBase	csa_circ_002464
Alias	Chr4:6967292_6973892
Organism	Cucumis sativus
Position	chrChr4: 6967292-6973892 JBrowse»
Reference genome	Cucumis sativus v1.5
Type	e-circRNA
Identification method	Find_circ
Parent gene	Csa4G108510
Parent gene annotation	NA
Parent gene strand	-
Alternative splicing	Chr4_circ_ig.30 Chr4_circ_ig.31 Chr4_circ_ig.32 Chr4_circ_ig.33 Csa4G108510_circ_g.1 Csa4G108510_circ_g.2 Csa4G108510_circ_g.3 Csa4G108510_circ_g.4
Support reads	NA
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Csa4G108510.1:9 Csa4G108510.2:9

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CGAAATCTGGGAACAAGGTACAAACCACTTTCTTTTAAACGATTGCGCTTTTCTTCCATCTTCT
TTTCAAAAACCATGAATATGTTGTCTCTTCCAGAATcttcaaactatgaccattaacagctcga
gcagcttccacaattgccttcccaactggatgaactgtgttagattccactgcagcagcaaact
tgagaatc

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	CGAAATCTGGGAACAAGGTACAAACCACTTTCTTTTAAACGATTGCGCTTTTCTTCCATCTTCT TTTCAAAAACCATGAATATGTTGTCTCTTCCAGAATcttcaaactatgaccattaacagctcga gcagcttccacaattgccttcccaactggatgaactgtgttagattccactgcagcagcaaact tgagaatc
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.098988445

Conservation Information
Conserved circRNAs	NA
PMCS	0.098988445

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							6973871-6973829(-)
						
						Potential amino acid sequence
						
							MVFEKKMEEKRNRLKESGRNLVFSWALCAVCLLGHISHFFGAKASWIHTFHTTQFHLSLCLFTL
LGPGRQLIIDGMKSLVKGAPNMNTLVGLGALSSFSVSSLAALMPKLGWKAFFEEPVMLIAFVLL
GRNLEQRAKIRAASDMTGLLSILPSKARLVVDGDTELSSTVEIPCSSLSIGDEVIVLPGDRIPA
DGIVKSGRSIVDESSFTGEPLPVTKLPGSQVAAGTINLNGTLTVKVHRQGGDTAMGDIIRLVEE
AQSREAPVQRLADKVSGHFTYGVMVLSAATFIFWSQFGSRILPAAFYHGSSVSLALQLSCSVLV
VACPCALGLATPTAMLVGTSLGATKGLLLRGGNILEQFSMVDTVVFDKTGTLTVGRPVVTKVFA
TSRYERNVDTQTNSHGNYSENEILKFAAAVESNTVHPVGKAIVEAARAVNGHSLKILEETTYSW
FLKRRWKKSAIV*
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	6973871-6973829(-)
Potential amino acid sequence	MVFEKKMEEKRNRLKESGRNLVFSWALCAVCLLGHISHFFGAKASWIHTFHTTQFHLSLCLFTL LGPGRQLIIDGMKSLVKGAPNMNTLVGLGALSSFSVSSLAALMPKLGWKAFFEEPVMLIAFVLL GRNLEQRAKIRAASDMTGLLSILPSKARLVVDGDTELSSTVEIPCSSLSIGDEVIVLPGDRIPA DGIVKSGRSIVDESSFTGEPLPVTKLPGSQVAAGTINLNGTLTVKVHRQGGDTAMGDIIRLVEE AQSREAPVQRLADKVSGHFTYGVMVLSAATFIFWSQFGSRILPAAFYHGSSVSLALQLSCSVLV VACPCALGLATPTAMLVGTSLGATKGLLLRGGNILEQFSMVDTVVFDKTGTLTVGRPVVTKVFA TSRYERNVDTQTNSHGNYSENEILKFAAAVESNTVHPVGKAIVEAARAVNGHSLKILEETTYSW FLKRRWKKSAIV*
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						He et al., 2020

circRNA

Detail information of Csa4G108510_circ_g.5

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