Plant Circular RNA Database

Detail information of Solyc12g098710.1_circ_g.1

			
						 General Information
					
						CircRNA Name
						Solyc12g098710.1_circ_g.1
					
						ID in PlantcircBase
						sly_circ_003690
					
						Alias
						12:64468838|64469326
					
						Organism
						
							Solanum lycopersicum						
					
						Position
						
							chr12: 66127788-66128276  JBrowse»						
					
						Reference genome
						
							SL2.50.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Solyc12g098710.1						
					
						Parent gene annotation
						
							protein_coding
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						43
					
						Tissues
						fruit
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Solyc12g098710.1.1:2

General Information
CircRNA Name	Solyc12g098710.1_circ_g.1
ID in PlantcircBase	sly_circ_003690
Alias	12:64468838\|64469326
Organism	Solanum lycopersicum
Position	chr12: 66127788-66128276 JBrowse»
Reference genome	SL2.50.38
Type	e-circRNA
Identification method	CIRI
Parent gene	Solyc12g098710.1
Parent gene annotation	protein_coding
Parent gene strand	+
Alternative splicing	NA
Support reads	43
Tissues	fruit
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Solyc12g098710.1.1:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: TATGAGGATTACCAGGCATC
 R: CCATAACTGCACTCCATCGT
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: TATGAGGATTACCAGGCATC R: CCATAACTGCACTCCATCGT PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ATACCCGTCGACATTCAATTTACTAGAGGTAGCGGCTGTTGACAAGCCCAAGATGCATCTTTGG
GAAACTGGGATTATGAGGATTACCAGGCATCCACAGattgtaatgctttcccttaccttgattt
tcgctatagtccacagtggtcttgctagtcttagagacaaaggtgaggaactcattggagagcg
tgcttttc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 357
Transcript exons: 66127788-66127934,66128067-66128276
ATTGTAATGCTTTCCCTTACCTTGATTTTCGCTATAGTCCACAGTGGTCTTGCTAGTCTTAGAG
ACAAAGGTGAGGAACTCATTGGAGAGCGTGCTTTTCGTGTATTGTTTGCTGGGGTATCTCTGCC
ATTGGCAGTCAGCACAATTGTGTATTTCATTAACCACCGATACGATGGAGTGCAGTTATGGCAA
TTAAACAGTGTTGCTGGGATTCACGAACTAGTTTGGATTTCTAACTTTGTTTCCTTCTTCTTCC
TATACCCGTCGACATTCAATTTACTAGAGGTAGCGGCTGTTGACAAGCCCAAGATGCATCTTTG
GGAAACTGGGATTATGAGGATTACCAGGCATCCACAG
						
						Genomic sequence
						
							ATTGTAATGCTTTCCCTTACCTTGATTTTCGCTATAGTCCACAGTGGTCTTGCTAGTCTTAGAG
ACAAAGGTGAGGAACTCATTGGAGAGCGTGCTTTTCGTGTATTGTTTGCTGGGGTATCTCTGCC
ATTGGCAGTCAGCACAATTGTGAGTTTTCTAGTCTGCCATGCAGAAACTAAGAATTTTGTTTAT
TTTTTTGGGTAACTTTTCTGATTTTACGGTGCGATGCTTGATAGATGGAAATTTAACATAACGG
AAGGTTCTTATGATGATTTACAGGTGTATTTCATTAACCACCGATACGATGGAGTGCAGTTATG
GCAATTAAACAGTGTTGCTGGGATTCACGAACTAGTTTGGATTTCTAACTTTGTTTCCTTCTTC
TTCCTATACCCGTCGACATTCAATTTACTAGAGGTAGCGGCTGTTGACAAGCCCAAGATGCATC
TTTGGGAAACTGGGATTATGAGGATTACCAGGCATCCACAG

Sequences
Splice junction sequence	ATACCCGTCGACATTCAATTTACTAGAGGTAGCGGCTGTTGACAAGCCCAAGATGCATCTTTGG GAAACTGGGATTATGAGGATTACCAGGCATCCACAGattgtaatgctttcccttaccttgattt tcgctatagtccacagtggtcttgctagtcttagagacaaaggtgaggaactcattggagagcg tgcttttc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 357 Transcript exons: 66127788-66127934,66128067-66128276 ATTGTAATGCTTTCCCTTACCTTGATTTTCGCTATAGTCCACAGTGGTCTTGCTAGTCTTAGAG ACAAAGGTGAGGAACTCATTGGAGAGCGTGCTTTTCGTGTATTGTTTGCTGGGGTATCTCTGCC ATTGGCAGTCAGCACAATTGTGTATTTCATTAACCACCGATACGATGGAGTGCAGTTATGGCAA TTAAACAGTGTTGCTGGGATTCACGAACTAGTTTGGATTTCTAACTTTGTTTCCTTCTTCTTCC TATACCCGTCGACATTCAATTTACTAGAGGTAGCGGCTGTTGACAAGCCCAAGATGCATCTTTG GGAAACTGGGATTATGAGGATTACCAGGCATCCACAG
Genomic sequence	ATTGTAATGCTTTCCCTTACCTTGATTTTCGCTATAGTCCACAGTGGTCTTGCTAGTCTTAGAG ACAAAGGTGAGGAACTCATTGGAGAGCGTGCTTTTCGTGTATTGTTTGCTGGGGTATCTCTGCC ATTGGCAGTCAGCACAATTGTGAGTTTTCTAGTCTGCCATGCAGAAACTAAGAATTTTGTTTAT TTTTTTGGGTAACTTTTCTGATTTTACGGTGCGATGCTTGATAGATGGAAATTTAACATAACGG AAGGTTCTTATGATGATTTACAGGTGTATTTCATTAACCACCGATACGATGGAGTGCAGTTATG GCAATTAAACAGTGTTGCTGGGATTCACGAACTAGTTTGGATTTCTAACTTTGTTTCCTTCTTC TTCCTATACCCGTCGACATTCAATTTACTAGAGGTAGCGGCTGTTGACAAGCCCAAGATGCATC TTTGGGAAACTGGGATTATGAGGATTACCAGGCATCCACAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.451336282

Conservation Information
Conserved circRNAs	NA
PMCS	0.451336282

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							66127795-66128273(+)
66128079-66128134(-)
						
						Potential amino acid sequence
						
							MLSLTLIFAIVHSGLASLRDKGEELIGERAFRVLFAGVSLPLAVSTIVYFINHRYDGVQLWQLN
SVAGIHELVWISNFVSFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQIVMLSLTLIFA
IVHSGLASLRDKGEELIGERAFRVLFAGVSLPLAVSTIVYFINHRYDGVQLWQLNSVAGIHELV
WISNFVSFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQIVMLSLTLIFAIVHSGLASL
RDKGEELIGERAFRVLFAGVSLPLAVSTIVYFINHRYDGVQLWQLNSVAGIHELVWISNFVSFF
FLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQIVMLSLTLIFAIVHSGLASLRDKGEELIG
ERAFRVLFAGVSLPLAVSTIVYFINHRYDGVQLWQLNSVAGIHELVWISNFVSFFFLYPSTFNL
LEVAAVDKPKMHLWETGIMRITRHPQ(+)
MKYTIVLTANGRDTPANNTRKARSPMSSSPLSLRLARPLWTIAKIKVRESITICGCLVILIIPV
SQRCILGLSTAATSSKLNVDGYRKKKETKLEIQTSS*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						fruit ripening

Functional Information
Coding potential	Y
Potential coding position	66127795-66128273(+) 66128079-66128134(-)
Potential amino acid sequence	MLSLTLIFAIVHSGLASLRDKGEELIGERAFRVLFAGVSLPLAVSTIVYFINHRYDGVQLWQLN SVAGIHELVWISNFVSFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQIVMLSLTLIFA IVHSGLASLRDKGEELIGERAFRVLFAGVSLPLAVSTIVYFINHRYDGVQLWQLNSVAGIHELV WISNFVSFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQIVMLSLTLIFAIVHSGLASL RDKGEELIGERAFRVLFAGVSLPLAVSTIVYFINHRYDGVQLWQLNSVAGIHELVWISNFVSFF FLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQIVMLSLTLIFAIVHSGLASLRDKGEELIG ERAFRVLFAGVSLPLAVSTIVYFINHRYDGVQLWQLNSVAGIHELVWISNFVSFFFLYPSTFNL LEVAAVDKPKMHLWETGIMRITRHPQ(+) MKYTIVLTANGRDTPANNTRKARSPMSSSPLSLRLARPLWTIAKIKVRESITICGCLVILIIPV SQRCILGLSTAATSSKLNVDGYRKKKETKLEIQTSS*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	fruit ripening

			
						 Other Information
					
						References
						Yin et al., 2018

circRNA

Detail information of Solyc12g098710.1_circ_g.1

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