Plant Circular RNA Database

Detail information of Os04g0418000_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os04g0418000_circ_g.2
					
						ID in PlantcircBase
						osa_circ_023801
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr4: 20655255-20656743  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						circseq_cup
					
						Parent gene
						
							Os04g0418000						
					
						Parent gene annotation
						
							Conserved hypothetical protein. (Os04t0418000-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os04g0418000_circ_g.3 Os04g0418000_circ_g.4 Os04g0418000_circ_g.5 						
					
						Support reads
						3
					
						Tissues
						root
					
						Exon boundary  
						
							No-No							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							TC-AT							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os04t0418000-01:2

General Information
CircRNA Name	Os04g0418000_circ_g.2
ID in PlantcircBase	osa_circ_023801
Alias	NA
Organism	Oryza sativa
Position	chr4: 20655255-20656743 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	circseq_cup
Parent gene	Os04g0418000
Parent gene annotation	Conserved hypothetical protein. (Os04t0418000-01)
Parent gene strand	+
Alternative splicing	Os04g0418000_circ_g.3 Os04g0418000_circ_g.4 Os04g0418000_circ_g.5
Support reads	3
Tissues	root
Exon boundary	No-No "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	TC-AT The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os04t0418000-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TTAGAAGGACAAATCTATATCCAACACCTGAAGCTCATGATTTATTTATTTCACTTGCAGATCA
CCCTGATTGGCACCTCAGGCATCTCAGGATCCTATGatctggaatcatttggcccggaggaaaa
ttatctacgtaagaaagtggagaatgagttggggactaaaatgatacacctgaaaatgcggtgc
agtggtgt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 159
Transcript exons: 20655255-20656743
ATCTGGAATCATTTGGCCCGGAGGAAAATTATCTACGTAAGAAAGTGGAGAATGAGTTGGGGAC
TAAAATGATACACCTGAAAATGCGGTGCAGTGGTGTAGGGTCCGAGTGGGGAAAGATCACCCTG
ATTGGCACCTCAGGCATCTCAGGATCCTATG
						
					

						Genomic sequence
						
							ATCTGGAATCATTTGGCCCGGAGGAAAATTATCTACGTAAGAAAGTGGAGAATGAGTTGGGGAC
TAAAATGATACACCTGAAAATGCGGTGCAGTGGTGTAGGGTCCGAGTGGGGAAAGGTACACTCT
TATGTTACCTATCCCTGTTGTATATTTTACCTTTGTGACAGCTTCTGTGGTAGATTTGCATTTG
ACAAGTCCATGGATTGTGGGTCCTGATGTATGCTTTAGTGCGGATTTATGCTATCATTTTTAAT
GGTTTCTTATGATTGTATTCAAGATGTTGTGTAGATGTTATTTTCAGCGTAGAAGCAGAGTTAG
TATGTGCAGTTTCTGCTATCACAAATGTGTTATTTGTGTTTAAGAATATATTCCTTTCTATGGC
TAAGGATCATTTAATATCAGACTGCATCTTGGGTCATCACTTGTCATTGGAGTAGGTTGTACTA
TGTCAGGACAGTCCTTTATTATTATTTTGCTTGATACAAAGGGAGAAATGAGATTCTACATCCA
TCTTTTTACTTGACGATTAGAACATTGTCACAGCCTCCAGGGCTCATGTTTGATCATATCTTTT
CTAATTGAATATGTATATAAAAATCATTAAAACTAAAACTACATTAAAGTGTTTTTCACGACAA
ATCTACTACAGTCATTTTCACATCAAATCTTAATGTTTTTGCAAATATCCGTGATTTAGGATTT
TGAGGTTTGACCACATACATCCTAAAAAATCATGTATTTGAGAACGGAAGGAGTAGCTTAGAGT
GAATATAAACACCTCAACCTCATCCAAAAAAACCAAGTGAATGAACTAGGACCATCATCTTGTC
TATCTATTCATTTGGTTTGAAGAAGGGAAATTGCACAGGTAAGTTGAGATTTGAGAACAATTGC
AGCGCATTCTCATTTTACCAAGTCTTGTGCTAGATATACTTCAAATAAATGTAAATGTAAAATG
GAAGCTAAATCAGTTTATCCTGCACAGCTATACACTGTGTAAGGCTCGGTAATAAAAGGTACAT
GCTTGTTATTTTGTTGTTTGCTTGCACAGAGACCTGTAGTACTTCCAATTGAGCACTGGCATAC
ACCCCTGGTACCATGTAACATGTTAACTTTCAAGAAGGGGGTATGCTTATCTTATTTTATCTGT
AATTAGTCAAAAAAAAAGGAGCTGTTCATTTACCATTGTCACCACTTACTTCAAACCTTATAAG
GGCTTATAGGGGTGTTTGCTTCCAGCCAATTTGGAACATATGATTTAGACCGAGTTTGAATTGT
TTTCAAATTAAAATAGACGAGTTCATGCTTGACGTTGTAACATGTGTGGAATAATATTTTCATG
TCAGTGTATAGCTGATATGCTCGTTCCTCCTTGCTTCGACTGGAGTTAGAAGGACAAATCTATA
TCCAACACCTGAAGCTCATGATTTATTTATTTCACTTGCAGATCACCCTGATTGGCACCTCAGG
CATCTCAGGATCCTATG
						
					

			
		

Sequences
Splice junction sequence	TTAGAAGGACAAATCTATATCCAACACCTGAAGCTCATGATTTATTTATTTCACTTGCAGATCA CCCTGATTGGCACCTCAGGCATCTCAGGATCCTATGatctggaatcatttggcccggaggaaaa ttatctacgtaagaaagtggagaatgagttggggactaaaatgatacacctgaaaatgcggtgc agtggtgt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 159 Transcript exons: 20655255-20656743 ATCTGGAATCATTTGGCCCGGAGGAAAATTATCTACGTAAGAAAGTGGAGAATGAGTTGGGGAC TAAAATGATACACCTGAAAATGCGGTGCAGTGGTGTAGGGTCCGAGTGGGGAAAGATCACCCTG ATTGGCACCTCAGGCATCTCAGGATCCTATG
Genomic sequence	ATCTGGAATCATTTGGCCCGGAGGAAAATTATCTACGTAAGAAAGTGGAGAATGAGTTGGGGAC TAAAATGATACACCTGAAAATGCGGTGCAGTGGTGTAGGGTCCGAGTGGGGAAAGGTACACTCT TATGTTACCTATCCCTGTTGTATATTTTACCTTTGTGACAGCTTCTGTGGTAGATTTGCATTTG ACAAGTCCATGGATTGTGGGTCCTGATGTATGCTTTAGTGCGGATTTATGCTATCATTTTTAAT GGTTTCTTATGATTGTATTCAAGATGTTGTGTAGATGTTATTTTCAGCGTAGAAGCAGAGTTAG TATGTGCAGTTTCTGCTATCACAAATGTGTTATTTGTGTTTAAGAATATATTCCTTTCTATGGC TAAGGATCATTTAATATCAGACTGCATCTTGGGTCATCACTTGTCATTGGAGTAGGTTGTACTA TGTCAGGACAGTCCTTTATTATTATTTTGCTTGATACAAAGGGAGAAATGAGATTCTACATCCA TCTTTTTACTTGACGATTAGAACATTGTCACAGCCTCCAGGGCTCATGTTTGATCATATCTTTT CTAATTGAATATGTATATAAAAATCATTAAAACTAAAACTACATTAAAGTGTTTTTCACGACAA ATCTACTACAGTCATTTTCACATCAAATCTTAATGTTTTTGCAAATATCCGTGATTTAGGATTT TGAGGTTTGACCACATACATCCTAAAAAATCATGTATTTGAGAACGGAAGGAGTAGCTTAGAGT GAATATAAACACCTCAACCTCATCCAAAAAAACCAAGTGAATGAACTAGGACCATCATCTTGTC TATCTATTCATTTGGTTTGAAGAAGGGAAATTGCACAGGTAAGTTGAGATTTGAGAACAATTGC AGCGCATTCTCATTTTACCAAGTCTTGTGCTAGATATACTTCAAATAAATGTAAATGTAAAATG GAAGCTAAATCAGTTTATCCTGCACAGCTATACACTGTGTAAGGCTCGGTAATAAAAGGTACAT GCTTGTTATTTTGTTGTTTGCTTGCACAGAGACCTGTAGTACTTCCAATTGAGCACTGGCATAC ACCCCTGGTACCATGTAACATGTTAACTTTCAAGAAGGGGGTATGCTTATCTTATTTTATCTGT AATTAGTCAAAAAAAAAGGAGCTGTTCATTTACCATTGTCACCACTTACTTCAAACCTTATAAG GGCTTATAGGGGTGTTTGCTTCCAGCCAATTTGGAACATATGATTTAGACCGAGTTTGAATTGT TTTCAAATTAAAATAGACGAGTTCATGCTTGACGTTGTAACATGTGTGGAATAATATTTTCATG TCAGTGTATAGCTGATATGCTCGTTCCTCCTTGCTTCGACTGGAGTTAGAAGGACAAATCTATA TCCAACACCTGAAGCTCATGATTTATTTATTTCACTTGCAGATCACCCTGATTGGCACCTCAGG CATCTCAGGATCCTATG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.099003878

Conservation Information
Conserved circRNAs	NA
PMCS	0.099003878

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							osa-miR1857-5p 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	osa-miR1857-5p
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2016

circRNA

Detail information of Os04g0418000_circ_g.2

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