Plant Circular RNA Database

Detail information of Os04g0592700_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os04g0592700_circ_g.1
					
						ID in PlantcircBase
						osa_circ_025031
					
						Alias
						Os_ciR884
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr4: 29982443-29982821  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, circseq_cup, find_circ
					
						Parent gene
						
							Os04g0592700						
					
						Parent gene annotation
						
							WD40 repeat-like domain containing protein. (Os04t0592700-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os04g0592700_circ_g.2 						
					
						Support reads
						29/2/5
					
						Tissues
						root/root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os04t0592700-01:2

General Information
CircRNA Name	Os04g0592700_circ_g.1
ID in PlantcircBase	osa_circ_025031
Alias	Os_ciR884
Organism	Oryza sativa
Position	chr4: 29982443-29982821 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, circseq_cup, find_circ
Parent gene	Os04g0592700
Parent gene annotation	WD40 repeat-like domain containing protein. (Os04t0592700-01)
Parent gene strand	-
Alternative splicing	Os04g0592700_circ_g.2
Support reads	29/2/5
Tissues	root/root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os04t0592700-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							Yes							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							F: AGGCAAGCTCCTCAATACCC
 R: AGCTTGCCTCCCACTGATAT
PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	Yes Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	F: AGGCAAGCTCCTCAATACCC R: AGCTTGCCTCCCACTGATAT PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TGTGGGGTCTTCTAAGCTCAAATGCAAGTTGTATTGCTTTTGCATAGTCATAATCTGATACTGC
ATTTTCTAATTCCTGACCTCTCAACAGTTCCTCCTCctccaggatatcagtgggaggcaagctc
ctcaatacccgaaaaagcacaaactgtgcaacatgacaaagcttaggttttgtgttccattccc
ggatatac

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 154
Transcript exons: 29982443-29982523,29982749-29982821
CTCCAGGATATCAGTGGGAGGCAAGCTCCTCAATACCCGAAAAAGCACAAACTGTGCAACATGA
CAAAGCTTAGGTTTTGTGTTGTATTGCTTTTGCATAGTCATAATCTGATACTGCATTTTCTAAT
TCCTGACCTCTCAACAGTTCCTCCTC
Transcript length: 306
Transcript exons: 29982443-29982617,29982691-29982821
CTCCAGGATATCAGTGGGAGGCAAGCTCCTCAATACCCGAAAAAGCACAAACTGTGCAACATGA
CAAAGCTTAGGTTTTGTGTTCCATTCCCGGATATACTCAAGAAGTACGCGAAGCCCTTCCTTTG
GAAGACCAATAAGGGCTTTTTCTATTGGGTCCTCCAGATCAGACTCCCTGCAAAGCTGTGTGAA
TAACTCAAGAAGCCTGTGGGGTCTTCTAAGCTCAAATGCAAGTTGTATTGCTTTTGCATAGTCA
TAATCTGATACTGCATTTTCTAATTCCTGACCTCTCAACAGTTCCTCCTC
						
						Genomic sequence
						
							CTCCAGGATATCAGTGGGAGGCAAGCTCCTCAATACCCGAAAAAGCACAAACTGTGCAACATGA
CAAAGCTTAGGTTTTGTGTTCCATTCCCGGATATACTCAAGAAGTACGCGAAGCCCTTCCTTTG
GAAGACCAATAAGGGCTTTTTCTATTGGGTCCTCCAGATCAGACTCCCTGCACACTCATGTGAT
ATATCAATTTGTTATCAAAGAAAAACTAGTGAGCTTCAATAAAGGATTATGAGTACCTGCAAAG
CTGTGTGAATAACTCAAGAAGCCTGTGGGGTCTTCTAAGCTCAAATGCAAGTTGTATTGCTTTT
GCATAGTCATAATCTGATACTGCATTTTCTAATTCCTGACCTCTCAACAGTTCCTCCTC

Sequences
Splice junction sequence	TGTGGGGTCTTCTAAGCTCAAATGCAAGTTGTATTGCTTTTGCATAGTCATAATCTGATACTGC ATTTTCTAATTCCTGACCTCTCAACAGTTCCTCCTCctccaggatatcagtgggaggcaagctc ctcaatacccgaaaaagcacaaactgtgcaacatgacaaagcttaggttttgtgttccattccc ggatatac
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 154 Transcript exons: 29982443-29982523,29982749-29982821 CTCCAGGATATCAGTGGGAGGCAAGCTCCTCAATACCCGAAAAAGCACAAACTGTGCAACATGA CAAAGCTTAGGTTTTGTGTTGTATTGCTTTTGCATAGTCATAATCTGATACTGCATTTTCTAAT TCCTGACCTCTCAACAGTTCCTCCTC Transcript length: 306 Transcript exons: 29982443-29982617,29982691-29982821 CTCCAGGATATCAGTGGGAGGCAAGCTCCTCAATACCCGAAAAAGCACAAACTGTGCAACATGA CAAAGCTTAGGTTTTGTGTTCCATTCCCGGATATACTCAAGAAGTACGCGAAGCCCTTCCTTTG GAAGACCAATAAGGGCTTTTTCTATTGGGTCCTCCAGATCAGACTCCCTGCAAAGCTGTGTGAA TAACTCAAGAAGCCTGTGGGGTCTTCTAAGCTCAAATGCAAGTTGTATTGCTTTTGCATAGTCA TAATCTGATACTGCATTTTCTAATTCCTGACCTCTCAACAGTTCCTCCTC
Genomic sequence	CTCCAGGATATCAGTGGGAGGCAAGCTCCTCAATACCCGAAAAAGCACAAACTGTGCAACATGA CAAAGCTTAGGTTTTGTGTTCCATTCCCGGATATACTCAAGAAGTACGCGAAGCCCTTCCTTTG GAAGACCAATAAGGGCTTTTTCTATTGGGTCCTCCAGATCAGACTCCCTGCACACTCATGTGAT ATATCAATTTGTTATCAAAGAAAAACTAGTGAGCTTCAATAAAGGATTATGAGTACCTGCAAAG CTGTGTGAATAACTCAAGAAGCCTGTGGGGTCTTCTAAGCTCAAATGCAAGTTGTATTGCTTTT GCATAGTCATAATCTGATACTGCATTTTCTAATTCCTGACCTCTCAACAGTTCCTCCTC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_005695* osi_circ_014723 						
					
						PMCS
						0.563239226

Conservation Information
Conserved circRNAs	osi_circ_005695* osi_circ_014723
PMCS	0.563239226

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							29982744-29982513(+)
						
						Potential amino acid sequence
						
							MQVVLLLHSHNLILHFLIPDLSTVPPPPGYQWEASSSIPEKAQTVQHDKA*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	29982744-29982513(+)
Potential amino acid sequence	MQVVLLLHSHNLILHFLIPDLSTVPPPPGYQWEASSSIPEKAQTVQHDKA*(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Ye et al., 2016;Chu et al., 2017

circRNA

Detail information of Os04g0592700_circ_g.1

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