Plant Circular RNA Database

Detail information of Os05g0465100_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os05g0465100_circ_g.2
					
						ID in PlantcircBase
						osa_circ_028180
					
						Alias
						Os_ciR9960
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 22836270-22838652  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Os05g0465100						
					
						Parent gene annotation
						
							RabGAP/TBC domain containing protein. (Os05t0465100-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os05g0465100_circ_g.1 Os05g0465100_circ_g.3 						
					
						Support reads
						2
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0465250-00:7
Os05t0465100-01:7
Os05t0465250-00:7

General Information
CircRNA Name	Os05g0465100_circ_g.2
ID in PlantcircBase	osa_circ_028180
Alias	Os_ciR9960
Organism	Oryza sativa
Position	chr5: 22836270-22838652 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	find_circ
Parent gene	Os05g0465100
Parent gene annotation	RabGAP/TBC domain containing protein. (Os05t0465100-01)
Parent gene strand	-
Alternative splicing	Os05g0465100_circ_g.1 Os05g0465100_circ_g.3
Support reads	2
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0465250-00:7 Os05t0465100-01:7 Os05t0465250-00:7

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GAAGCATTCCCAGTACCATAAAACTCAAGTAGTGCAAGAGCTGTCCGGAATAGCATCACACGAT
TCCCATCAAACAGAAGGACATCCCAGACACGGAGAActtggagaaggcaagggggaaagaagag
ctttctgctggctagtttgatactgcagtgtggcctccaacattgtctctgccattacagccct
gttctcca

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 949
Transcript exons: 22836270-22836406,22836888-22836989,22837066-22837146,22
837470-22837539,22837773-22837834,22838053-22838465,2283
8569-22838652
CTTGGAGAAGGCAAGGGGGAAAGAAGAGCTTTCTGCTGGCTAGTTTGATACTGCAGTGTGGCCT
CCAACATTGTCTCTGCCATTACAGCCCTGTTCTCCATTTTCGCGAGTGAAGCCATAGCTTCCTC
ATACTTTTCCTGCAGCACATTAACAGCGTATCTCTGAGCTGCGGCATCCTGCTCAGCAGAGATG
CGTGCTTCCTCTGCAATTTTCAGTTCTTGTTCCACTCGCAACAGTACCTGACACATTGCTTGCT
CTTGTTCCTGCTTATCCAATAGGGTTTGTCTTAGTTCAGACAATTCTTGTTCCGACTGCTCAAC
CTTTGCACTTAATTGCCGCCTATTATCTTGCTTGACCATTTCCATCAATGCCGTCTCTAGTTCA
TCAGCTCTGAGAACAGCTGACCTTCTCTCCTCTAGCAATCGGCATAATTCAAACTTCAGCCAAG
AGATCTGGTCCTTTGGGTCCGGTAAAGCTTCAATCTCAGAACTGACTGTCAGACCGCCATACAT
ATCATCCATATTTCCAGAATCACTTTGGTTGGCATTCCCATCTCCCACATCACTCATCTGATCT
TGCGAATCACTTATTGACACCAGTGGTTCAGGATCATGTTTGAAATTATAGAGCTTTGATGCAA
GACCGTTGGTGTCCAAGGCACCTAGACCTTTTGCTCTTTCTTCCATTGAAGATAGAACAGATGG
CCGATGCTTATTCCTTAACTCTTGCAATCCTGTTTCATCTACAGACTGATATCCCATGCGAGCT
GTCAAGACAAGCTGGCTGCTGTCAAAAGTGGAGCCAGCTAAAGACTGCAAAAGTGTAACTGCAT
CACCGGCATCTTTTGTGGTCACAAGTGCAGGACCATAAAACTCAAGTAGTGCAAGAGCTGTCCG
GAATAGCATCACACGATTCCCATCAAACAGAAGGACATCCCAGACACGGAGAA
						
					

						Genomic sequence
						
							CTTGGAGAAGGCAAGGGGGAAAGAAGAGCTTTCTGCTGGCTAGTTTGATACTGCAGTGTGGCCT
CCAACATTGTCTCTGCCATTACAGCCCTGTTCTCCATTTTCGCGAGTGAAGCCATAGCTTCCTC
ATACTTTTCCTGCATATGTGTCAGGATCCGATGAGAGCCCAATATTACAAAAATATAGAAAATA
AGTATACAAATATGTTTTAAGGGCACAACATATGTATATGTCACAGTATTTGCATGTGTGCATG
TAAATGTACGTGACTACCGTTTTGGTTAGTTCAGATAAAAATACTCCCTCCAGTTATAGATATT
TGATGCTTAGGACAAGATTTTGATCAAACTTTTAAAATTCCGACCATGAAATTTCTTAAATACT
TAGTTTTAAAACAAAAAAATGATACACGTATATTTGCTTCAAAAAGTACTATCATAATATCATA
AGCTTATTGGATTTTATAAACTTATTCTAATATAAAATTGATTGTTGGAATTTATAAGTTTGAC
TGAACCTTGTCCTGAGTGTCAAATATTTAGGACCGGAAGGAGTAAGTGATTTTAAGAGAGAAAA
TATGATCTCAGAAGGATGGACCGAGCTTGTTGACTGACTAACCTGCAGCACATTAACAGCGTAT
CTCTGAGCTGCGGCATCCTGCTCAGCAGAGATGCGTGCTTCCTCTGCAATTTTCAGTTCTTGTT
CCACTCGCAACAGTACCTTCAATATAATTTCTGGTGAGTGGACATGCCATGGTAGAAAGATACA
TCTATAAAAACTAATGGGTCAACTGTACCTGACACATTGCTTGCTCTTGTTCCTGCTTATCCAA
TAGGGTTTGTCTTAGTTCAGACAATTCTTGTTCCGACTGCTCAACCTAAAAGAGAAGAGGGACA
TTCAAACACACTTATGTTAGAAAATTAGATATCTTTAGCTCATAGGATGAATTATGATATACTG
GTGTTGGTTATGATTTGGATATTTCAAAATAAAGTGCCTCTACTAGATAAATCTTGCACCATTT
ATAATTTGGTAGAATGATTATTTCTAAAACACTGTTATATATACTTGAAATAAAGAACATGGTG
AAATTTATTTCTGTGGCATAGTTGCATTGTAAACGCCATCTCTGAACTTGCAATCTGACTATTG
GCACAAAGGAACACCTCCTTATACAAAAGTTTAGTTATCTGGCTATACCTTTGCACTTAATTGC
CGCCTATTATCTTGCTTGACCATTTCCATCAATGCCGTCTCTAGTTCATCAGCTCTGGATATCA
AAAAGAAGAAAATTGGAATTAATACTTCAGACTGGTAGACAACCAGATCAGGACTCAAGGACTG
ATACGGGTAAATTAGCCATAAATCTATTTAAAAGCAAAGTCGCATAAGATGGTTCCAGCAACCT
ATGACAGAATGCATGTATATTTCTCATTTTGAAATAATGAAAAACGACTTTCCAGCAAATTATG
ACAATATCAAATTATGAAATGTAAAACATACCTGAGAACAGCTGACCTTCTCTCCTCTAGCAAT
CGGCATAATTCAAACTTCAGCCAAGAGATCTAATGTAAGAAGAGTAAATCAGACAAGAAGATCT
GCTGTAGTAAAACTGATTCCCATAGAAGATATGAAGTAATAAAAATAATTCAGATAAAGGATAT
GCAGTAATGAAACTGGTTCGTATCAGAAGATCTGCAGGGATTCAAACTAGAAACTACAGTAGCC
ACTTCAGGTATAGAGAACAATTTACTAAATCTAATTACTATGATGAGATGGCTACCTGGTCCTT
TGGGTCCGGTAAAGCTTCAATCTCAGAACTGACTGTCAGACCGCCATACATATCATCCATATTT
CCAGAATCACTTTGGTTGGCATTCCCATCTCCCACATCACTCATCTGATCTTGCGAATCACTTA
TTGACACCAGTGGTTCAGGATCATGTTTGAAATTATAGAGCTTTGATGCAAGACCGTTGGTGTC
CAAGGCACCTAGACCTTTTGCTCTTTCTTCCATTGAAGATAGAACAGATGGCCGATGCTTATTC
CTTAACTCTTGCAATCCTGTTTCATCTACAGACTGATATCCCATGCGAGCTGTCAAGACAAGCT
GGCTGCTGTCAAAAGTGGAGCCAGCTAAAGACTGCAAAAGTGTAACTGCATCACCGGCATCTTT
TGTGGTCACAAGTGCAGGACCTGCAATCAAGAAAAATATCAGTACCTAGCTAGCCCACGAGATG
AAACTACTTATTGTCGTTTTATTTTAGGAAACTATGAGAATCAGAAGCATTCCCAGTACCATAA
AACTCAAGTAGTGCAAGAGCTGTCCGGAATAGCATCACACGATTCCCATCAAACAGAAGGACAT
CCCAGACACGGAGAA
						
					

			
		

Sequences
Splice junction sequence	GAAGCATTCCCAGTACCATAAAACTCAAGTAGTGCAAGAGCTGTCCGGAATAGCATCACACGAT TCCCATCAAACAGAAGGACATCCCAGACACGGAGAActtggagaaggcaagggggaaagaagag ctttctgctggctagtttgatactgcagtgtggcctccaacattgtctctgccattacagccct gttctcca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 949 Transcript exons: 22836270-22836406,22836888-22836989,22837066-22837146,22 837470-22837539,22837773-22837834,22838053-22838465,2283 8569-22838652 CTTGGAGAAGGCAAGGGGGAAAGAAGAGCTTTCTGCTGGCTAGTTTGATACTGCAGTGTGGCCT CCAACATTGTCTCTGCCATTACAGCCCTGTTCTCCATTTTCGCGAGTGAAGCCATAGCTTCCTC ATACTTTTCCTGCAGCACATTAACAGCGTATCTCTGAGCTGCGGCATCCTGCTCAGCAGAGATG CGTGCTTCCTCTGCAATTTTCAGTTCTTGTTCCACTCGCAACAGTACCTGACACATTGCTTGCT CTTGTTCCTGCTTATCCAATAGGGTTTGTCTTAGTTCAGACAATTCTTGTTCCGACTGCTCAAC CTTTGCACTTAATTGCCGCCTATTATCTTGCTTGACCATTTCCATCAATGCCGTCTCTAGTTCA TCAGCTCTGAGAACAGCTGACCTTCTCTCCTCTAGCAATCGGCATAATTCAAACTTCAGCCAAG AGATCTGGTCCTTTGGGTCCGGTAAAGCTTCAATCTCAGAACTGACTGTCAGACCGCCATACAT ATCATCCATATTTCCAGAATCACTTTGGTTGGCATTCCCATCTCCCACATCACTCATCTGATCT TGCGAATCACTTATTGACACCAGTGGTTCAGGATCATGTTTGAAATTATAGAGCTTTGATGCAA GACCGTTGGTGTCCAAGGCACCTAGACCTTTTGCTCTTTCTTCCATTGAAGATAGAACAGATGG CCGATGCTTATTCCTTAACTCTTGCAATCCTGTTTCATCTACAGACTGATATCCCATGCGAGCT GTCAAGACAAGCTGGCTGCTGTCAAAAGTGGAGCCAGCTAAAGACTGCAAAAGTGTAACTGCAT CACCGGCATCTTTTGTGGTCACAAGTGCAGGACCATAAAACTCAAGTAGTGCAAGAGCTGTCCG GAATAGCATCACACGATTCCCATCAAACAGAAGGACATCCCAGACACGGAGAA
Genomic sequence	CTTGGAGAAGGCAAGGGGGAAAGAAGAGCTTTCTGCTGGCTAGTTTGATACTGCAGTGTGGCCT CCAACATTGTCTCTGCCATTACAGCCCTGTTCTCCATTTTCGCGAGTGAAGCCATAGCTTCCTC ATACTTTTCCTGCATATGTGTCAGGATCCGATGAGAGCCCAATATTACAAAAATATAGAAAATA AGTATACAAATATGTTTTAAGGGCACAACATATGTATATGTCACAGTATTTGCATGTGTGCATG TAAATGTACGTGACTACCGTTTTGGTTAGTTCAGATAAAAATACTCCCTCCAGTTATAGATATT TGATGCTTAGGACAAGATTTTGATCAAACTTTTAAAATTCCGACCATGAAATTTCTTAAATACT TAGTTTTAAAACAAAAAAATGATACACGTATATTTGCTTCAAAAAGTACTATCATAATATCATA AGCTTATTGGATTTTATAAACTTATTCTAATATAAAATTGATTGTTGGAATTTATAAGTTTGAC TGAACCTTGTCCTGAGTGTCAAATATTTAGGACCGGAAGGAGTAAGTGATTTTAAGAGAGAAAA TATGATCTCAGAAGGATGGACCGAGCTTGTTGACTGACTAACCTGCAGCACATTAACAGCGTAT CTCTGAGCTGCGGCATCCTGCTCAGCAGAGATGCGTGCTTCCTCTGCAATTTTCAGTTCTTGTT CCACTCGCAACAGTACCTTCAATATAATTTCTGGTGAGTGGACATGCCATGGTAGAAAGATACA TCTATAAAAACTAATGGGTCAACTGTACCTGACACATTGCTTGCTCTTGTTCCTGCTTATCCAA TAGGGTTTGTCTTAGTTCAGACAATTCTTGTTCCGACTGCTCAACCTAAAAGAGAAGAGGGACA TTCAAACACACTTATGTTAGAAAATTAGATATCTTTAGCTCATAGGATGAATTATGATATACTG GTGTTGGTTATGATTTGGATATTTCAAAATAAAGTGCCTCTACTAGATAAATCTTGCACCATTT ATAATTTGGTAGAATGATTATTTCTAAAACACTGTTATATATACTTGAAATAAAGAACATGGTG AAATTTATTTCTGTGGCATAGTTGCATTGTAAACGCCATCTCTGAACTTGCAATCTGACTATTG GCACAAAGGAACACCTCCTTATACAAAAGTTTAGTTATCTGGCTATACCTTTGCACTTAATTGC CGCCTATTATCTTGCTTGACCATTTCCATCAATGCCGTCTCTAGTTCATCAGCTCTGGATATCA AAAAGAAGAAAATTGGAATTAATACTTCAGACTGGTAGACAACCAGATCAGGACTCAAGGACTG ATACGGGTAAATTAGCCATAAATCTATTTAAAAGCAAAGTCGCATAAGATGGTTCCAGCAACCT ATGACAGAATGCATGTATATTTCTCATTTTGAAATAATGAAAAACGACTTTCCAGCAAATTATG ACAATATCAAATTATGAAATGTAAAACATACCTGAGAACAGCTGACCTTCTCTCCTCTAGCAAT CGGCATAATTCAAACTTCAGCCAAGAGATCTAATGTAAGAAGAGTAAATCAGACAAGAAGATCT GCTGTAGTAAAACTGATTCCCATAGAAGATATGAAGTAATAAAAATAATTCAGATAAAGGATAT GCAGTAATGAAACTGGTTCGTATCAGAAGATCTGCAGGGATTCAAACTAGAAACTACAGTAGCC ACTTCAGGTATAGAGAACAATTTACTAAATCTAATTACTATGATGAGATGGCTACCTGGTCCTT TGGGTCCGGTAAAGCTTCAATCTCAGAACTGACTGTCAGACCGCCATACATATCATCCATATTT CCAGAATCACTTTGGTTGGCATTCCCATCTCCCACATCACTCATCTGATCTTGCGAATCACTTA TTGACACCAGTGGTTCAGGATCATGTTTGAAATTATAGAGCTTTGATGCAAGACCGTTGGTGTC CAAGGCACCTAGACCTTTTGCTCTTTCTTCCATTGAAGATAGAACAGATGGCCGATGCTTATTC CTTAACTCTTGCAATCCTGTTTCATCTACAGACTGATATCCCATGCGAGCTGTCAAGACAAGCT GGCTGCTGTCAAAAGTGGAGCCAGCTAAAGACTGCAAAAGTGTAACTGCATCACCGGCATCTTT TGTGGTCACAAGTGCAGGACCTGCAATCAAGAAAAATATCAGTACCTAGCTAGCCCACGAGATG AAACTACTTATTGTCGTTTTATTTTAGGAAACTATGAGAATCAGAAGCATTCCCAGTACCATAA AACTCAAGTAGTGCAAGAGCTGTCCGGAATAGCATCACACGATTCCCATCAAACAGAAGGACAT CCCAGACACGGAGAA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.233996272

Conservation Information
Conserved circRNAs	NA
PMCS	0.233996272

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							22838610-22838624(-)
						
						Potential amino acid sequence
						
							MLFRTALALLEFYGPALVTTKDAGDAVTLLQSLAGSTFDSSQLVLTARMGYQSVDETGLQELRN
KHRPSVLSSMEERAKGLGALDTNGLASKLYNFKHDPEPLVSISDSQDQMSDVGDGNANQSDSGN
MDDMYGGLTVSSEIEALPDPKDQISWLKFELCRLLEERRSAVLRADELETALMEMVKQDNRRQL
SAKVEQSEQELSELRQTLLDKQEQEQAMCQVLLRVEQELKIAEEARISAEQDAAAQRYAVNVLQ
EKYEEAMASLAKMENRAVMAETMLEATLQYQTSQQKALLSPLPSPSSPCLGCPSV*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	22838610-22838624(-)
Potential amino acid sequence	MLFRTALALLEFYGPALVTTKDAGDAVTLLQSLAGSTFDSSQLVLTARMGYQSVDETGLQELRN KHRPSVLSSMEERAKGLGALDTNGLASKLYNFKHDPEPLVSISDSQDQMSDVGDGNANQSDSGN MDDMYGGLTVSSEIEALPDPKDQISWLKFELCRLLEERRSAVLRADELETALMEMVKQDNRRQL SAKVEQSEQELSELRQTLLDKQEQEQAMCQVLLRVEQELKIAEEARISAEQDAAAQRYAVNVLQ EKYEEAMASLAKMENRAVMAETMLEATLQYQTSQQKALLSPLPSPSSPCLGCPSV*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of Os05g0465100_circ_g.2

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