Plant Circular RNA Database

Detail information of Os03g0401300_circ_g.12

			
						 General Information
					
						CircRNA Name
						Os03g0401300_circ_g.12
					
						ID in PlantcircBase
						osa_circ_020230
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 16303061-16303427  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os03g0401300						
					
						Parent gene annotation
						
							Sucrose synthase 2 (EC 2.4.1.13) (Sucrose-UDP glucosyltransferas
e 2). (Os03t0401300-01);Similar to Sucrose synthase 1. (Os03t040
1300-02);Similar to Sucrose synthase metabolism (Fragment). (Os0
3t0401300-03)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os03g0401300_circ_g.13 Os03g0401300_circ_g.14 Os03g0401300_circ_g.15 Os03g0401300_circ_g.16 Os03g0401300_circ_g.17 Os03g0401300_circ_g.18 Os03g0401300_circ_g.19 Os03g0401300_circ_g.20 Os03g0401300_circ_g.21 Os03g0401300_circ_g.22 Os03g0401300_circ_g.23 Os03g0401300_circ_g.24 Os03g0401300_circ_g.25 Os03g0401300_circ_g.26 Os03g0401300_circ_g.27 						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0401333-00:2
Os03t0401333-00:2
Os03t0401300-01:2

General Information
CircRNA Name	Os03g0401300_circ_g.12
ID in PlantcircBase	osa_circ_020230
Alias	NA
Organism	Oryza sativa
Position	chr3: 16303061-16303427 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os03g0401300
Parent gene annotation	Sucrose synthase 2 (EC 2.4.1.13) (Sucrose-UDP glucosyltransferas e 2). (Os03t0401300-01);Similar to Sucrose synthase 1. (Os03t040 1300-02);Similar to Sucrose synthase metabolism (Fragment). (Os0 3t0401300-03)
Parent gene strand	-
Alternative splicing	Os03g0401300_circ_g.13 Os03g0401300_circ_g.14 Os03g0401300_circ_g.15 Os03g0401300_circ_g.16 Os03g0401300_circ_g.17 Os03g0401300_circ_g.18 Os03g0401300_circ_g.19 Os03g0401300_circ_g.20 Os03g0401300_circ_g.21 Os03g0401300_circ_g.22 Os03g0401300_circ_g.23 Os03g0401300_circ_g.24 Os03g0401300_circ_g.25 Os03g0401300_circ_g.26 Os03g0401300_circ_g.27
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0401333-00:2 Os03t0401333-00:2 Os03t0401300-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TGTGTGCAAGCAAGCATGCAACAAGGTTTCCGTCACTGTAGTTTCCGATGATCAAGTCAGGATT
GGCCTGGAGCTCTCCAGCAATTTCGTGCGCGACATCtttccggcaatctcttggaaggtactgg
tgatgatgaagtcagcgtggttcatcgcaatcaagtcagttgtgaactggcatgagaagtggta
gtgatcct

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 284
Transcript exons: 16303061-16303227,16303311-16303427
TTTCCGGCAATCTCTTGGAAGGTACTGGTGATGATGAAGTCAGCGTGGTTCATCGCAATCAAGT
CAGTTGTGAACTGGCATGAGAAGTGGTAGTGATCCTCAAACTTCTTCCAGTAGAGGTCAGAGTT
GGGGTACTTGGTTTTCTCAAGCGCATGGGCAATGGTACAATGGGTAACACCCATCTTGTGTGCA
AGCAAGCATGCAACAAGGTTTCCGTCACTGTAGTTTCCGATGATCAAGTCAGGATTGGCCTGGA
GCTCTCCAGCAATTTCGTGCGCGACATC
						
						Genomic sequence
						
							TTTCCGGCAATCTCTTGGAAGGTACTGGTGATGATGAAGTCAGCGTGGTTCATCGCAATCAAGT
CAGTTGTGAACTGGCATGAGAAGTGGTAGTGATCCTCAAACTTCTTCCAGTAGAGGTCAGAGTT
GGGGTACTTGGTTTTCTCAAGCGCATGGGCAATGGTACACTGCATATCATGGAACAAGGCAACA
TGAGAATGAGCAAATATGTAGTTAAAACACTTCTGATCAGAGTAGTCAAAATGCGTACATGGGT
AACACCCATCTTGTGTGCAAGCAAGCATGCAACAAGGTTTCCGTCACTGTAGTTTCCGATGATC
AAGTCAGGATTGGCCTGGAGCTCTCCAGCAATTTCGTGCGCGACATC

Sequences
Splice junction sequence	TGTGTGCAAGCAAGCATGCAACAAGGTTTCCGTCACTGTAGTTTCCGATGATCAAGTCAGGATT GGCCTGGAGCTCTCCAGCAATTTCGTGCGCGACATCtttccggcaatctcttggaaggtactgg tgatgatgaagtcagcgtggttcatcgcaatcaagtcagttgtgaactggcatgagaagtggta gtgatcct
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 284 Transcript exons: 16303061-16303227,16303311-16303427 TTTCCGGCAATCTCTTGGAAGGTACTGGTGATGATGAAGTCAGCGTGGTTCATCGCAATCAAGT CAGTTGTGAACTGGCATGAGAAGTGGTAGTGATCCTCAAACTTCTTCCAGTAGAGGTCAGAGTT GGGGTACTTGGTTTTCTCAAGCGCATGGGCAATGGTACAATGGGTAACACCCATCTTGTGTGCA AGCAAGCATGCAACAAGGTTTCCGTCACTGTAGTTTCCGATGATCAAGTCAGGATTGGCCTGGA GCTCTCCAGCAATTTCGTGCGCGACATC
Genomic sequence	TTTCCGGCAATCTCTTGGAAGGTACTGGTGATGATGAAGTCAGCGTGGTTCATCGCAATCAAGT CAGTTGTGAACTGGCATGAGAAGTGGTAGTGATCCTCAAACTTCTTCCAGTAGAGGTCAGAGTT GGGGTACTTGGTTTTCTCAAGCGCATGGGCAATGGTACACTGCATATCATGGAACAAGGCAACA TGAGAATGAGCAAATATGTAGTTAAAACACTTCTGATCAGAGTAGTCAAAATGCGTACATGGGT AACACCCATCTTGTGTGCAAGCAAGCATGCAACAAGGTTTCCGTCACTGTAGTTTCCGATGATC AAGTCAGGATTGGCCTGGAGCTCTCCAGCAATTTCGTGCGCGACATC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.538554905

Conservation Information
Conserved circRNAs	NA
PMCS	0.538554905

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							16303376-16303088(+)
16303214-16303150(+)
16303327-16303381(-)
						
						Potential amino acid sequence
						
							MIKSGLAWSSPAISCATSFRQSLGRYW*(+)
MGNGTMGNTHLVCKQACNKVSVTVVSDDQVRIGLELSSNFVRDIFPAISWKVLVMMKSAWFIAI
KSVVNWHEKW*(+)
MGVTHCTIAHALEKTKYPNSDLYWKKFEDHYHFSCQFTTDLIAMNHADFIITSTFQEIAGKMSR
TKLLESSRPILT*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	16303376-16303088(+) 16303214-16303150(+) 16303327-16303381(-)
Potential amino acid sequence	MIKSGLAWSSPAISCATSFRQSLGRYW(+) MGNGTMGNTHLVCKQACNKVSVTVVSDDQVRIGLELSSNFVRDIFPAISWKVLVMMKSAWFIAI KSVVNWHEKW(+) MGVTHCTIAHALEKTKYPNSDLYWKKFEDHYHFSCQFTTDLIAMNHADFIITSTFQEIAGKMSR TKLLESSRPILT*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os03g0401300_circ_g.12

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