Plant Circular RNA Database

Detail information of Os05g0277300_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os05g0277300_circ_g.4
					
						ID in PlantcircBase
						osa_circ_027222
					
						Alias
						Os05circ06346
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 11438605-11440298  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						SMALT, Segemehl
					
						Parent gene
						
							Os05g0277300						
					
						Parent gene annotation
						
							Similar to Peptide chain release factor 1. (Os05t0277300-01);Pep
tide chain release factor 1 family protein. (Os05t0277300-02);No
n-protein coding transcript. (Os05t0277300-03)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os05g0277300_circ_g.1 Os05g0277300_circ_g.2 Os05g0277300_circ_g.3 Os05g0277300_circ_g.5 Os05g0277300_circ_g.6 						
					
						Support reads
						2
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-No							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0277300-02:3
Os05t0277300-01:3

General Information
CircRNA Name	Os05g0277300_circ_g.4
ID in PlantcircBase	osa_circ_027222
Alias	Os05circ06346
Organism	Oryza sativa
Position	chr5: 11438605-11440298 JBrowse»
Reference genome	IRGSP-1.0.38
Type	u-circRNA
Identification method	SMALT, Segemehl
Parent gene	Os05g0277300
Parent gene annotation	Similar to Peptide chain release factor 1. (Os05t0277300-01);Pep tide chain release factor 1 family protein. (Os05t0277300-02);No n-protein coding transcript. (Os05t0277300-03)
Parent gene strand	-
Alternative splicing	Os05g0277300_circ_g.1 Os05g0277300_circ_g.2 Os05g0277300_circ_g.3 Os05g0277300_circ_g.5 Os05g0277300_circ_g.6
Support reads	2
Tissues	leaf
Exon boundary	Yes-No "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0277300-02:3 Os05t0277300-01:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AAAGGGTAAATAGAAGAGAAACTCAAAAATACCACAACATTCCTAATCTCCTCTTCCTCTCCTC
TATAGCTTGTTGCCTGCCTCTGCCTGCCTTCAGTCCctgcacggacctccaatatgcaatccct
ctcgtcagcttcatcttttggaagtaatgatcgaaacagcttgtgctgcagttgtttctcctcc
tccagagc

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							CTGCACGGACCTCCAATATGCAATCCCTCTCGTCAGCTTCATCTTTTGGAAGTAATGATCGAAA
CAGCTTGTGCTGCAGTTGTTTCTCCTCCTCCAGAGCCTGAAGGAGCTCCTGAGCTGCCATCTCG
CGGAAGTCCTTCTCTTCAACAGAGTTTGTCATCAAGGATTTCAAACCCTCAATTTCCTGCAAAT
ACTCAAATAGTTTGGCCTACTCCCAGTCTACCATACCATGGGATGTGAACATTACACAAATTCA
TGTGATCATTACCTCCTGTTTGGACCGCAGCTCCTCGATCATATCCATAGTACTCTCCAATTTG
TGGAGCTCCTTGTTGGCCCTTGAGTATTCTTCAGGCGAGGCATCTGGCTGTCTTCATTAACGTC
AAACTCAAATCAAGATTATTTTTACTTCCAACCTAAAATTTGAAAGCTCTACATGGCCACATGA
TAACAAGAACTGAACAGTGCTAGAAACAGAGGTTGCCCATCCAAAGATGACCTGGTTAATTTGC
TCCTGGAGGTACGCACTCCTCTGCTCGATCAATTTCATTCTCTGCTCCATGACCCCCACCAGGT
TCGCAGGAAGCTGCTGGTTCATCTCTGATGCGAAAAGAGGGGCAAACAAGGAGCACACTTGAAT
CAAATATTTCTTCTTTGCAGCAGCACAGTCATATTATCATAGATGAACCAGAAAGCAGAGAAAG
AGTGGAGTAAGCAGAGATGGAGCAAGAAGATGTGACAGCAGACATTGGCTCACCATTGGTGGAG
TATAGCCGAGGAGAAGGGCACTTCCATCCAATTGGAGGTGACGTGGATACAGCATGCGGAAAGT
GGCAGAAACATCGGACCATGGTGAAAGCAGCAGCACCACATCGACGTAGGTGATGTTTCATCAT
GCTGGTGGAGTGAGGCAGGCTTGGAAATGGGAATCTATGGCTGGGGTTTGGTGGCCATCTTCTT
CTCGCTTCTGAAAGAAGTTTGTGTAACCCAGTAGGACACCTTCGACGATGCTTATTTGAATGCT
GTCTCAGATCGAGAGCATGTTATCACCACAGATCACGTAATTTGCTTCAGACCTAGCATCCATT
ATTCAACAAAATTCCTGAATTAAATTAAATAATAATGAATAACAGTTGAAGCATAACAACATAA
ACTTTCTGCATTGAATCTATTCAGACATTTCATCCTAAAGGATTTATTTCTTCTGACAGTACAT
ATATTGCCCATAGAAGATACTAACAATACTGAATTAGTGATTCTACTAGCAAAAGAACAAGTTG
ATGATATGGAATAGGAGAGGTGAAGATGGACAGAACCTAAGAGCATCAAGTAGTGGCACAACCA
GAGCCCAAACAGATACCAAGAGCAAATTCTACATGTCTTCATGCAGACAAATACAAACAAGGCT
TAAATTTATCAAAAGTACACTGAAATAATTGGACGACCGAAAAAAAAAATAGGTGTGGCACATG
AACTAGCGTGAGAATTAGAACAGAACAAGAAGGGAAAAAAAAGCAACATTATCCCATTATGTCT
ACATTAAACATAGCAACAAGCACTAGTCATAAGATCACAATTCATCATCCAGTTGCCAAAAGGG
TAAATAGAAGAGAAACTCAAAAATACCACAACATTCCTAATCTCCTCTTCCTCTCCTCTATAGC
TTGTTGCCTGCCTCTGCCTGCCTTCAGTCC
						
					

			
		

Sequences
Splice junction sequence	AAAGGGTAAATAGAAGAGAAACTCAAAAATACCACAACATTCCTAATCTCCTCTTCCTCTCCTC TATAGCTTGTTGCCTGCCTCTGCCTGCCTTCAGTCCctgcacggacctccaatatgcaatccct ctcgtcagcttcatcttttggaagtaatgatcgaaacagcttgtgctgcagttgtttctcctcc tccagagc
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CTGCACGGACCTCCAATATGCAATCCCTCTCGTCAGCTTCATCTTTTGGAAGTAATGATCGAAA CAGCTTGTGCTGCAGTTGTTTCTCCTCCTCCAGAGCCTGAAGGAGCTCCTGAGCTGCCATCTCG CGGAAGTCCTTCTCTTCAACAGAGTTTGTCATCAAGGATTTCAAACCCTCAATTTCCTGCAAAT ACTCAAATAGTTTGGCCTACTCCCAGTCTACCATACCATGGGATGTGAACATTACACAAATTCA TGTGATCATTACCTCCTGTTTGGACCGCAGCTCCTCGATCATATCCATAGTACTCTCCAATTTG TGGAGCTCCTTGTTGGCCCTTGAGTATTCTTCAGGCGAGGCATCTGGCTGTCTTCATTAACGTC AAACTCAAATCAAGATTATTTTTACTTCCAACCTAAAATTTGAAAGCTCTACATGGCCACATGA TAACAAGAACTGAACAGTGCTAGAAACAGAGGTTGCCCATCCAAAGATGACCTGGTTAATTTGC TCCTGGAGGTACGCACTCCTCTGCTCGATCAATTTCATTCTCTGCTCCATGACCCCCACCAGGT TCGCAGGAAGCTGCTGGTTCATCTCTGATGCGAAAAGAGGGGCAAACAAGGAGCACACTTGAAT CAAATATTTCTTCTTTGCAGCAGCACAGTCATATTATCATAGATGAACCAGAAAGCAGAGAAAG AGTGGAGTAAGCAGAGATGGAGCAAGAAGATGTGACAGCAGACATTGGCTCACCATTGGTGGAG TATAGCCGAGGAGAAGGGCACTTCCATCCAATTGGAGGTGACGTGGATACAGCATGCGGAAAGT GGCAGAAACATCGGACCATGGTGAAAGCAGCAGCACCACATCGACGTAGGTGATGTTTCATCAT GCTGGTGGAGTGAGGCAGGCTTGGAAATGGGAATCTATGGCTGGGGTTTGGTGGCCATCTTCTT CTCGCTTCTGAAAGAAGTTTGTGTAACCCAGTAGGACACCTTCGACGATGCTTATTTGAATGCT GTCTCAGATCGAGAGCATGTTATCACCACAGATCACGTAATTTGCTTCAGACCTAGCATCCATT ATTCAACAAAATTCCTGAATTAAATTAAATAATAATGAATAACAGTTGAAGCATAACAACATAA ACTTTCTGCATTGAATCTATTCAGACATTTCATCCTAAAGGATTTATTTCTTCTGACAGTACAT ATATTGCCCATAGAAGATACTAACAATACTGAATTAGTGATTCTACTAGCAAAAGAACAAGTTG ATGATATGGAATAGGAGAGGTGAAGATGGACAGAACCTAAGAGCATCAAGTAGTGGCACAACCA GAGCCCAAACAGATACCAAGAGCAAATTCTACATGTCTTCATGCAGACAAATACAAACAAGGCT TAAATTTATCAAAAGTACACTGAAATAATTGGACGACCGAAAAAAAAAATAGGTGTGGCACATG AACTAGCGTGAGAATTAGAACAGAACAAGAAGGGAAAAAAAAGCAACATTATCCCATTATGTCT ACATTAAACATAGCAACAAGCACTAGTCATAAGATCACAATTCATCATCCAGTTGCCAAAAGGG TAAATAGAAGAGAAACTCAAAAATACCACAACATTCCTAATCTCCTCTTCCTCTCCTCTATAGC TTGTTGCCTGCCTCTGCCTGCCTTCAGTCC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.100716652

Conservation Information
Conserved circRNAs	NA
PMCS	0.100716652

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015

circRNA

Detail information of Os05g0277300_circ_g.4

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