Plant Circular RNA Database

Detail information of AT1G54100_circ_g.3

			
						 General Information
					
						CircRNA Name
						AT1G54100_circ_g.3
					
						ID in PlantcircBase
						ath_circ_007261
					
						Alias
						AT1G54100_C1, CircALDH7B4
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr1: 20195580-20195917  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI2
					
						Parent gene
						
							AT1G54100						
					
						Parent gene annotation
						
							ALDH7B4
							
						Parent gene strand
						-
					
						Alternative splicing
						
							AT1G54100_circ_g.1 AT1G54100_circ_g.2 AT1G54100_circ_g.4 AT1G54100_circ_g.5 AT1G54100_circ_g.6 AT1G54100_circ_g.7 AT1G54100_circ_g.8 AT1G54100_circ_g.9 AT1G54100_circ_g.10 AT1G54100_circ_g.11 AT1G54100_circ_g.12 AT1G54100_circ_g.13 AT1G54100_circ_g.14 AT1G54100_circ_g.15 AT1G54100_circ_g.16 AT1G54100_circ_g.17 AT1G54100_circ_g.18 AT1G54100_circ_g.19 						
					
						Support reads
						NA
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT1G54100.2:2
AT1G54100.1:2

General Information
CircRNA Name	AT1G54100_circ_g.3
ID in PlantcircBase	ath_circ_007261
Alias	AT1G54100_C1, CircALDH7B4
Organism	Arabidpsis thaliana
Position	chr1: 20195580-20195917 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRI2
Parent gene	AT1G54100
Parent gene annotation	ALDH7B4
Parent gene strand	-
Alternative splicing	AT1G54100_circ_g.1 AT1G54100_circ_g.2 AT1G54100_circ_g.4 AT1G54100_circ_g.5 AT1G54100_circ_g.6 AT1G54100_circ_g.7 AT1G54100_circ_g.8 AT1G54100_circ_g.9 AT1G54100_circ_g.10 AT1G54100_circ_g.11 AT1G54100_circ_g.12 AT1G54100_circ_g.13 AT1G54100_circ_g.14 AT1G54100_circ_g.15 AT1G54100_circ_g.16 AT1G54100_circ_g.17 AT1G54100_circ_g.18 AT1G54100_circ_g.19
Support reads	NA
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT1G54100.2:2 AT1G54100.1:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F:GCTTTTGGAGGCGAGAAAGC
 R:GACCTTGAGGAACCGAGTTGT
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F:GCTTTTGGAGGCGAGAAAGC R:GACCTTGAGGAACCGAGTTGT PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ACCCGATCCACCTGAAGATGTTTTCAGGGTTCCGAGTGAATATCGAGCTGCTTAGACCTTGAGG
AACCGAGTTGTTTATCGCAACAGCCTCTCCAAATGAcaagtcgatcgacgcatgtactgtttcc
atgagtcgcttccagcttcacgaccaccaccagtcgctttctcgcctccaaaagctccaccaat
ctcagctc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 248
Transcript exons: 20195580-20195729,20195820-20195917
CAAGTCGATCGACGCATGTACTGTTTCCATGAGTCGCTTCCAGCTTCACGACCACCACCAGTCG
CTTTCTCGCCTCCAAAAGCTCCACCAATCTCAGCTCCATTCGTCGGTATGTTCACATTCACAAT
GCCACAGTCACTTCCCAGTGGTCCGATCCACCTGAAGATGTTTTCAGGGTTCCGAGTGAATATC
GAGCTGCTTAGACCTTGAGGAACCGAGTTGTTTATCGCAACAGCCTCTCCAAATGA
						
						Genomic sequence
						
							CAAGTCGATCGACGCATGTACTGTTTCCATGAGTCGCTTCCAGCTTCACGACCACCACCAGTCG
CTTTCTCGCCTCCAAAAGCTCCACCAATCTCAGCTCCATTCGTCGGTATGTTCACATTCACAAT
GCCACAGTCACTTCCCAGTGGTCTAGTAGTAAAACCATCACATTAAAATTAATTTCAGTAGATT
ATATTGGTCGGAAAGATTGAGTTAAGTTGTTGTTTTTATAGCACTTACCCGATCCACCTGAAGA
TGTTTTCAGGGTTCCGAGTGAATATCGAGCTGCTTAGACCTTGAGGAACCGAGTTGTTTATCGC
AACAGCCTCTCCAAATGA

Sequences
Splice junction sequence	ACCCGATCCACCTGAAGATGTTTTCAGGGTTCCGAGTGAATATCGAGCTGCTTAGACCTTGAGG AACCGAGTTGTTTATCGCAACAGCCTCTCCAAATGAcaagtcgatcgacgcatgtactgtttcc atgagtcgcttccagcttcacgaccaccaccagtcgctttctcgcctccaaaagctccaccaat ctcagctc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 248 Transcript exons: 20195580-20195729,20195820-20195917 CAAGTCGATCGACGCATGTACTGTTTCCATGAGTCGCTTCCAGCTTCACGACCACCACCAGTCG CTTTCTCGCCTCCAAAAGCTCCACCAATCTCAGCTCCATTCGTCGGTATGTTCACATTCACAAT GCCACAGTCACTTCCCAGTGGTCCGATCCACCTGAAGATGTTTTCAGGGTTCCGAGTGAATATC GAGCTGCTTAGACCTTGAGGAACCGAGTTGTTTATCGCAACAGCCTCTCCAAATGA
Genomic sequence	CAAGTCGATCGACGCATGTACTGTTTCCATGAGTCGCTTCCAGCTTCACGACCACCACCAGTCG CTTTCTCGCCTCCAAAAGCTCCACCAATCTCAGCTCCATTCGTCGGTATGTTCACATTCACAAT GCCACAGTCACTTCCCAGTGGTCTAGTAGTAAAACCATCACATTAAAATTAATTTCAGTAGATT ATATTGGTCGGAAAGATTGAGTTAAGTTGTTGTTTTTATAGCACTTACCCGATCCACCTGAAGA TGTTTTCAGGGTTCCGAGTGAATATCGAGCTGCTTAGACCTTGAGGAACCGAGTTGTTTATCGC AACAGCCTCTCCAAATGA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.512373335

Conservation Information
Conserved circRNAs	NA
PMCS	0.512373335

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							20195684-20195843(-)
20195611-20195895(-)
						
						Potential amino acid sequence
						
							MELRLVELLEARKRLVVVVKLEATHGNSTCVDRLVIWRGCCDKQLGSSRSKQLDIHSEP*
METVHASIDLSFGEAVAINNSVPQGLSSSIFTRNPENIFRWIGPLGSDCGIVNVNIPTNGAEIG
GAFGGEKATGGGREAGSDSWKQYMRRSTCHLERLLR*
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						response to drought stress

Functional Information
Coding potential	Y
Potential coding position	20195684-20195843(-) 20195611-20195895(-)
Potential amino acid sequence	MELRLVELLEARKRLVVVVKLEATHGNSTCVDRLVIWRGCCDKQLGSSRSKQLDIHSEP* METVHASIDLSFGEAVAINNSVPQGLSSSIFTRNPENIFRWIGPLGSDCGIVNVNIPTNGAEIG GAFGGEKATGGGREAGSDSWKQYMRRSTCHLERLLR*
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	response to drought stress

			
						 Other Information
					
						References
						Zhang et al., 2019

circRNA

Detail information of AT1G54100_circ_g.3

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