Plant Circular RNA Database

Detail information of Os03g0738400_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os03g0738400_circ_g.3
					
						ID in PlantcircBase
						osa_circ_021771
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 30300468-30301147  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						PcircRNA_finder
					
						Parent gene
						
							Os03g0738400						
					
						Parent gene annotation
						
							Similar to Serine hydroxymethyltransferase, cytosolic (EC 2.1.2.
1) (Serine methylase) (Glycine hydroxymethyltransferase) (SHMT).
 (Os03t0738400-01);Similar to Serine hydroxymethyltransferase. (
Os03t0738400-02);Similar to Serine hydroxymethyltransferase. (Os
03t0738400-03)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os03g0738400_circ_g.4 Os03g0738400_circ_g.5 Os03g0738400_circ_g.6 Os03g0738400_circ_g.7 						
					
						Support reads
						9
					
						Tissues
						seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0738400-02:1
Os03t0738400-01:2
Os03t0738400-03:1

General Information
CircRNA Name	Os03g0738400_circ_g.3
ID in PlantcircBase	osa_circ_021771
Alias	NA
Organism	Oryza sativa
Position	chr3: 30300468-30301147 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	PcircRNA_finder
Parent gene	Os03g0738400
Parent gene annotation	Similar to Serine hydroxymethyltransferase, cytosolic (EC 2.1.2. 1) (Serine methylase) (Glycine hydroxymethyltransferase) (SHMT). (Os03t0738400-01);Similar to Serine hydroxymethyltransferase. ( Os03t0738400-02);Similar to Serine hydroxymethyltransferase. (Os 03t0738400-03)
Parent gene strand	+
Alternative splicing	Os03g0738400_circ_g.4 Os03g0738400_circ_g.5 Os03g0738400_circ_g.6 Os03g0738400_circ_g.7
Support reads	9
Tissues	seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0738400-02:1 Os03t0738400-01:2 Os03t0738400-03:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTTGGAT
CTTCCTCATGGTGGACATCTTTCTCACGGCTACCAGatacattgatatggccgagtcattgtgc
cagaaacgtgctttggaggccttccgcttggacccagcgaaatggggaggtaaatcattttcac
tctttatc

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							ATACATTGATATGGCCGAGTCATTGTGCCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCA
GCGAAATGGGGAGGTAAATCATTTTCACTCTTTATCTTGCCTTGTCATGTTTTTATATGAACTC
CAGCTTTCATGCGCATCAATAGCCTTTAGTCCTCTTTTGTTTCCTGCTTTGGGCGATGTAAATG
AAAAAAAAAAAGGATAGGGGCAAAAAGGTTCCTTTGGATAAGACACAGTTTTTATGGTTCCATA
GTTCACAAAAAAAAAAAAAGGGATTTTGATCAGAAAATTTCTTCTAGGTTCTTAATTTACTTAG
CTTCCTCACTAGTACAATGAAAAGATTACATCGTTTATAAACCACTCGGTTCTTCAGTAACCTG
AATACAAATGGTACACGAGTGGTCCTATTCATTTATATTTTTCGAATTGTTTGCTTTCATTATT
AACCGCTTTGTACTTAAATTTATCTAGAATACTTATTCTCAAAATTTTAGTTTTCTTTCTCCAA
AAAGGTGGCATTCTAACTTGACAACCAATTTCTACCTCTGCAGTGAATGTGCAACCTCTATCAG
GGTCACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTT
GGATCTTCCTCATGGTGGACATCTTTCTCACGGCTACCAG

Sequences
Splice junction sequence	ACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTTGGAT CTTCCTCATGGTGGACATCTTTCTCACGGCTACCAGatacattgatatggccgagtcattgtgc cagaaacgtgctttggaggccttccgcttggacccagcgaaatggggaggtaaatcattttcac tctttatc
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	ATACATTGATATGGCCGAGTCATTGTGCCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCA GCGAAATGGGGAGGTAAATCATTTTCACTCTTTATCTTGCCTTGTCATGTTTTTATATGAACTC CAGCTTTCATGCGCATCAATAGCCTTTAGTCCTCTTTTGTTTCCTGCTTTGGGCGATGTAAATG AAAAAAAAAAAGGATAGGGGCAAAAAGGTTCCTTTGGATAAGACACAGTTTTTATGGTTCCATA GTTCACAAAAAAAAAAAAAGGGATTTTGATCAGAAAATTTCTTCTAGGTTCTTAATTTACTTAG CTTCCTCACTAGTACAATGAAAAGATTACATCGTTTATAAACCACTCGGTTCTTCAGTAACCTG AATACAAATGGTACACGAGTGGTCCTATTCATTTATATTTTTCGAATTGTTTGCTTTCATTATT AACCGCTTTGTACTTAAATTTATCTAGAATACTTATTCTCAAAATTTTAGTTTTCTTTCTCCAA AAAGGTGGCATTCTAACTTGACAACCAATTTCTACCTCTGCAGTGAATGTGCAACCTCTATCAG GGTCACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTT GGATCTTCCTCATGGTGGACATCTTTCTCACGGCTACCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.207720355

Conservation Information
Conserved circRNAs	NA
PMCS	0.207720355

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							30300479-30300473(+)
30301090-30300473(+)
30300475-30301078(-)
						
						Potential amino acid sequence
						
							MAESLCQKRALEAFRLDPAKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSHGYQ
IH*(+)
MRESWLWIFLMVDIFLTATRYIDMAESLCQKRALEAFRLDPAKWGVNVQPLSGSPANFHVYTAL
LKPHERIMALDLPHGGHLSHGYQIH*(+)
MYLVAVRKMSTMRKIQSHDSLMWFQ*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	30300479-30300473(+) 30301090-30300473(+) 30300475-30301078(-)
Potential amino acid sequence	MAESLCQKRALEAFRLDPAKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSHGYQ IH(+) MRESWLWIFLMVDIFLTATRYIDMAESLCQKRALEAFRLDPAKWGVNVQPLSGSPANFHVYTAL LKPHERIMALDLPHGGHLSHGYQIH(+) MYLVAVRKMSTMRKIQSHDSLMWFQ*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os03g0738400_circ_g.3

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